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Development of A Super-Sensitive Immunolabeling Method Utilizing DNA Origami Molecular Machines

Research Project

Project/Area Number 19K22261
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 37:Biomolecular chemistry and related fields
Research InstitutionKansai University

Principal Investigator

Kuzuya Akinori  関西大学, 化学生命工学部, 教授 (00456154)

Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsDNAオリガミ / 人工抗体 / 分子機械
Outline of Research at the Start

本研究は、医療診断分野で広く用いられている抗体分子に関して、その製造コストの問題を解決するために、「抗体がもつ特長を全て備えつつ、さらにこれを凌駕した機能をもつ『人工抗体』を、タンパク質以外をつかって構築する」ことを目的とする。そのために、研究代表者が世界に先駆けて独自に開発した「ターゲット分子を挟むように結合することでX字型の開いた構造から=字型の閉じた構造に変形するDNAオリガミ分子機械(Nature Commun. 2011, 2, 449. DNA PliersとDNA Chopsticks)」を本体に使用することで、DNAでできた「人工抗体」を構築することをめざす。

Outline of Final Research Achievements

A "substrate recognition site" to recognize practical targets used in immunostaining and ELISA and a "signal transmission site" to detect structural changes as fluorescence and enzymatic activity were incorporated to Project Leader's original "DNA Origami molecular machine", to develop an "allosteric artificial antibody" that is unprecedented in the world. By employing novel operating mechanism of the DNA Origami molecular machine, selective on/off control of signal transmission was successfully accomplished.

Academic Significance and Societal Importance of the Research Achievements

今日までにがんの治療薬として「抗体医薬」の有用性が広く認知されてきたが、タンパク質である抗体分子の製造には大きなコストがかかるため、これらの普及は同時に、我が国の今後の医療費の高騰を引き起こす可能性があることが指摘されてきた。本研究が目的とする核酸ベースの人工抗体を実用化することができれば、抗体医薬の抗原認識部位だけを取り出して活用することで、非常に安価な後発医薬品を広範に提供できるプラットフォームを確立できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (13 results)

All 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] スイス連邦工科大学チューリッヒ校(スイス)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Single-Molecule AFM Study of DNA Damage by 1O2 Generated from Photoexcited C602020

    • Author(s)
      Ray Ankita、Liosi Korinne、Ramakrishna Shivaprakash N.、Spencer Nicholas D.、Kuzuya Akinori、Yamakoshi Yoko
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 11 Issue: 18 Pages: 7819-7826

    • DOI

      10.1021/acs.jpclett.0c02257

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Application of DNA Quadruplex Hydrogels Prepared from Polyethylene Glycol-Oligodeoxynucleotide Conjugates to Cell Culture Media2019

    • Author(s)
      Shizuma Tanaka, Shinsuke Yukami, Yuhei Hachiro, Yuichi Ohya, Akinori Kuzuya
    • Journal Title

      Polymers

      Volume: 11 Issue: 10 Pages: 1607-1607

    • DOI

      10.3390/polym11101607

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] DNA OrigamiのAFM観察を活用した単分子抗原検査法の開発2021

    • Author(s)
      南出悠貴、西山健太郎、仁木智哉、秋葉宏樹、永田諭志、鎌田春彦、大野浩章、葛谷明紀
    • Organizer
      第 5 回分子ロボティクス年次大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Visualization of Antigen Binding on DNA Origami Using Atomic Force Microscopy2021

    • Author(s)
      Yuki Minamide, Kentaro Nishiyama, Tomoya Niki, Hiroki Akiba, Satoshi Nagata, Haruhiko Kamada, Hiroaki Ohno, Akinori Kuzuya
    • Organizer
      CBI学会2021年大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Real-Time AFM Observation of DNA-Capped CyD Rotaxane Attached to a DNA Origami Pegboard2020

    • Author(s)
      Akinori Kuzuya
    • Organizer
      BIONANO2020 Workshop
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Real-Time Single-Molecular AFM Observation of DNA Origami Nanomachines2019

    • Author(s)
      Akinori Kuzuya
    • Organizer
      The 14th International Symposium in Science and Technology at Chulalongkorn University
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] DNA Quadruplex Hydrogels Prepared by Liquid-Phase Large Scale DNA Synthesis2019

    • Author(s)
      Akinori Kuzuya
    • Organizer
      Commemorative International Symposium of Nucleic Acid Chemistry (CISNAC 2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Remarks] 関西大学 化学生命工学部 知能分子学研究室

    • URL

      https://wps.itc.kansai-u.ac.jp/mol-mach/

    • Related Report
      2021 Annual Research Report 2020 Research-status Report 2019 Research-status Report
  • [Remarks] 関西大学 学術情報システム

    • URL

      https://gakujo.kansai-u.ac.jp/profile/ja/e43atXUd5P3aff3deIc240Ib6.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 関西大学 学術情報システム

    • URL

      http://gakujo.kansai-u.ac.jp/profile/ja/e43atXUd5P3aff3deIc240Ib6.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 関西大学 学術情報システム

    • URL

      http://gakujo.kansai-u.ac.jp/profile/ja/e43atXUd5P3aff3deIc240Ib6.html

    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] 核酸構造体2020

    • Inventor(s)
      葛谷明紀、高橋望、岡本祐太、河野幸子、加藤千尋、齋藤敬太
    • Industrial Property Rights Holder
      セルスペクト株式会社、関西大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-132829
    • Filing Date
      2020
    • Related Report
      2020 Research-status Report

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Published: 2019-07-04   Modified: 2023-01-30  

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