Does the membrane at focal adhesions differ from other membrane and regulate cellular function?
| Project/Area Number |
19K22279
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2019-06-28 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 接着斑 / 細胞膜 / コラーゲン / リン脂質 / 細胞外マトリックス / 脂質 |
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| Outline of Research at the Start |
コラーゲンなどの細胞外基質の種類や濃度、硬さが、炎症や脂肪細胞分化、がん細胞遊走を調節する。これまでに、細胞と細胞外基質との接着装置である接着斑に局在するタンパク質の重要性が示され、その機能が解明されてきた。本研究では、接着斑細胞膜が他の細胞膜に比べて特殊であり、その組成が変化することが重要であるという可能性を検証する。そのために、接着斑細胞膜の脂質組成を解析できる実験系と接着斑の細胞膜脂質組成を変化させる実験系の構築を目指す。
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| Outline of Final Research Achievements |
Focal adhesions are important for sensing the extracellular environment, including type, concentration, and stiffness of extracellular matrix, and thereby regulating cellular function. Although the functions of focal adhesion proteins have been well characterized, the plasma membrane within the focal adhesions is not well characterized. In this study, we investigated the lipids within the plasma membrane of focal adhesions. We established an experimental system for extracting lipids from isolated focal adhesion fractions and examined their lipid composition, suggesting that the plasma membrane of focal adhesions has a different composition from that of the entire plasma membrane. We also tried to establish an experimental system to modify the lipid composition of focal adhesion membrane.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞外環境は接着斑を介して、間葉系幹細胞の分化の方向性やがんの悪性化に影響を与えています。本研究により、接着斑領域にある細胞膜は他の細胞膜とは組成が異なることが示唆されました。このことは接着斑の働きに細胞膜が関与してる可能性を示しており、さらに今後接着斑細胞膜の重要性を示すことで、再生医療のための新しい仕組みの開発につながることが期待されます。
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Report
(5 results)
Research Products
(69 results)
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[Journal Article] Changes in the asymmetric distribution of cholesterol in the plasma membrane influence streptolysin O pore formation2019
Author(s)
Ogasawara, F., Kano, F., Murata, M., Kimura, Y., Kioka, N., Ueda, K
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Journal Title
Sci. Rep.
Volume: 9
Issue: 1
Pages: 4548-4548
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Roles of the Mesenchymal Stromal/Stem Cell Marker Meflin in Cardiac Tissue Repair and the Development of Diastolic Dysfunction.2019
Author(s)
Hara A, Kobayashi H, Asai N, Saito S, Higuchi T, Kato K, Okumura T, Bando YK, Takefuji M, Mizutani Y, Miyai Y, Saito S, Maruyama S, Maeda K, Ouchi N, Nagasaka A, Miyata T, Mii S, Kioka N, Worthley DL, Murohara T, Takahashi M, Enomoto A.
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Journal Title
Circ. Res.
Volume: 125
Issue: 4
Pages: 414-430
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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