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Analysis of the dynamics of plant lactic acid bacteria in nature and elucidation of the basis of disease suppression phenomenon in plants colonized by lactic acid bacteria

Research Project

Project/Area Number 19K22300
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionTohoku University

Principal Investigator

Takahashi Hideki  東北大学, 農学研究科, 教授 (20197164)

Co-Investigator(Kenkyū-buntansha) 北澤 春樹  東北大学, 農学研究科, 教授 (10204885)
Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords植物性乳酸菌 / 環境微生物 / 植物免疫 / 植物病害防除
Outline of Research at the Start

本研究では、緑色蛍光タンパク質遺伝子を形質転換した植物性乳酸菌を作出し、植物の茎葉、動物の糞便、その糞便を混和した培土で植物栽培した植物における乳酸菌の存在を検出するとともに、乳酸菌が定着している植物における病害抵抗性誘導と植物免疫システムの活性化を評価する。乳酸菌が、ヒトや家畜動物の腸管免疫免疫力を向上させることは広く認知されている。しかし植物に対しては、作物栽培における微生物農薬として実用化されているものの、病害抑制のメカニズムの理解は十分ではない。本研究により、乳酸菌が定着した植物における植物免疫システムの活性化の解析は、乳酸菌の病害抑制メカニズムの解明と、病害防除への応用に貢献する。

Outline of Final Research Achievements

It is known that plant lactic acid bacteria (Lactobacillus plantarum) activate the innate immune system of animals, contributing to the health of humans and livestock. In this study, we aimed to clarify the inhibitory effect of plant lactic acid bacteria isolated from the intestines of livestock against plant diseases. When plant lactic acid bacteria isolated from pig intestines and grass silage were sprayed onto the stems and leaves of tomato seedlings, and the roots of the seedlings were then inoculated with the tomato wilt bacteria, the occurrence of bacterial wilt symptoms was significantly suppressed. Since the distribution of plant lactic acid bacteria and the bacterial wilt bacteria in tomato plants is clearly different, it was thought that the plant lactic acid bacteria in the roots may induce disease resistance in plants by activating the plant immune system.

Academic Significance and Societal Importance of the Research Achievements

植物性乳酸菌は免疫システムを活性化させて、ヒトや家畜の健康に寄与することが知られている。また、植物の茎葉には植物性乳酸菌が定着しているが、植物性乳酸菌の植物への役割についての研究は不十分である。本研究では植物性乳酸菌を植物に施用して病害抑制効果を評価することにより、自然界における植物性乳酸菌の動態と植物や動物との関係の包括的理解しようとした。研究の結果、ブタ腸管から単離された植物性乳酸菌は、トマト青枯病の発病抑制効果を持つことが明らかになった。このことは、乳酸菌を生態系の構成因子として捉え、植物・動物との関わりについての包括的理解に繋がる。さらに乳酸菌の植物病害防除資材として利用が期待される。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (26 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (15 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 15 results,  Open Access: 11 results) Presentation (10 results)

  • [Int'l Joint Research] 国立乳酸菌研究所(アルゼンチン)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Isolation of Burkholderia jumbo phages and their utilization as biocontrol agents to suppress rice seedling rot disease2022

    • Author(s)
      Kanaizuka Ayane、Sasaki Ryota、Miyashita Shuhei、Ando Sugihiro、Ito Kumiko、Fukuhara Toshiyuki、Takahashi Hideki
    • Journal Title

      Journal of General Plant Pathology

      Volume: 89 Issue: 1 Pages: 24-34

    • DOI

      10.1007/s10327-022-01107-z

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppression of rice seedling rot caused by Burkholderia glumae in nursery soils using culturable bacterial communities from organic farming systems2022

    • Author(s)
      Ando Sugihiro、Kasahara Masami、Mitomi Naoto、Schermer Tom A.、Sato Erika、Yoshida Shigenobu、Tsushima Seiya、Miyashita Shuhei、Takahashi Hideki
    • Journal Title

      Journal of Plant Pathology

      Volume: - Issue: 2 Pages: 605-618

    • DOI

      10.1007/s42161-022-01066-6

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ligilactobacillus salivarius Strains Isolated From the Porcine Gut Modulate Innate Immune Responses in Epithelial Cells and Improve Protection Against Intestinal Viral-Bacterial Superinfection2021

    • Author(s)
      Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa
    • Journal Title

      Front Immunol

      Volume: 12 Pages: 652923-652923

    • DOI

      10.3389/fimmu.2021.652923

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vivo emergence of beige-like fat in chickens as physiological adaptation to cold environments2021

    • Author(s)
      Sotome Rina、Hirasawa Akira、Kikusato Motoi、Amo Taku、Furukawa Kyohei、Kuriyagawa Anna、Watanabe Kouichi、Collin Anne、Shirakawa Hitoshi、Hirakawa Ryota、Tanitaka Yuta、Takahashi Hideki、Wu Guoyao、Nochi Tomonori、Shimmura Tsuyoshi、Warden Craig H.、Toyomizu Masaaki
    • Journal Title

      Amino Acids

      Volume: 53 Issue: 3 Pages: 381-393

    • DOI

      10.1007/s00726-021-02953-5

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation and characterization of a novel jumbo phage from leaf litter compost and its suppressive effect on rice seedling rot diseases.2021

    • Author(s)
      Sasaki R, Miyashita S, Ando S, Ito K, Fukuhara T, Takahashi H
    • Journal Title

      Viruses

      Volume: 13 Issue: 4 Pages: 591-591

    • DOI

      10.3390/v13040591

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient Selection of New Immunobiotic Strains With Antiviral Effects in Local and Distal Mucosal Sites by Using Porcine Intestinal Epitheliocytes2020

    • Author(s)
      Leonardo, A., V. Garcia-Castillo, H. Kitazawa et al.
    • Journal Title

      Frontiers in Immunology

      Volume: 11:543 Pages: 1-16

    • DOI

      10.3389/fimmu.2020.00543

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exopolysaccharides from Streptococcus thermophilus ST538 modulate the antiviral innate immune response in porcine intestinal epitheliocytes.2020

    • Author(s)
      Mizuno H, Tomotsune K, Islam MA, Funabashi R, Albarracin L, Ikeda-Ohtsubo W, Aso H, Takahashi H, Kimura K, Villena J, Sasaki Y and Kitazawa H
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 894-894

    • DOI

      10.3389/fmicb.2020.00894

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lipoteichoic acid is involved in the ability of the immunobiotic strain Lactobacillus plantarum CRL1506 to modulate the intestinal antiviral innate immunity triggered by TLR3 activation.2020

    • Author(s)
      Hiroya Mizuno, Lorena Arce, Kae Tomotsune, Leonardo Albarracin, Ryutaro Funabashi, Daniela Vera, Md. Aminul Islam, Maria Guadalupe Vizoso-Pinto, Hideki Takahashi, Yasuko Sasaki, Haruki Kitazawa and Julio Villena
    • Journal Title

      Frontiers in Immunology

      Volume: 11 Pages: 571-571

    • DOI

      10.3389/fimmu.2020.00571

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Exopolysaccharide of Lactobacillus fermentum UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against Helicobacter pylori Infection2020

    • Author(s)
      Garcia-Castillo, V., H. Kitazawa et al.
    • Journal Title

      Microorganisms

      Volume: 8:479 Issue: 4 Pages: 1-20

    • DOI

      10.3390/microorganisms8040479

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of fat accumulation and adipokine production during the long-term adipogenic differentiation of porcine intramuscular preadipocytes and study of the influence of immunobiotics.2020

    • Author(s)
      Tada, A., Kober, A.H., Islam, M.A., Igata, M., Takagi, M., Suzuki, M., Aso, H., Ikeda-Ohtsubo, W., Yoda, K., Miyazawa, K., He, F., Takahashi, H., Villena, J. and Kitazawa, H.
    • Journal Title

      Cells

      Volume: 9 Issue: 7 Pages: 1715-1715

    • DOI

      10.3390/cells9071715

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deciphering the influence of paraimmunobiotic bifidobacteria on the innate antiviral immune response of bovine intestinal epitheliocytes by transcriptomic analysis2019

    • Author(s)
      Albarracin L.、Komatsu R.、Garcia-Castillo V.、Aso H.、Iwabuchi N.、Xiao J.-Z.、Abe F.、Takahashi H.、Villena J.、Kitazawa H.
    • Journal Title

      Beneficial Microbes

      Volume: 10 Issue: 2 Pages: 199-209

    • DOI

      10.3920/bm2018.0024

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation2019

    • Author(s)
      Igata Manami、Islam Md. Aminul、Tada Asuka、Takagi Michihiro、Kober A. K. M. Humayun、Albarracin Leonardo、Aso Hisashi、Ikeda-Ohtsubo Wakako、Miyazawa Kenji、Yoda Kazutoyo、He Fang、Takahashi Hideki、Villena Julio、Kitazawa Haruki
    • Journal Title

      Frontiers in Immunology

      Volume: 10 Pages: 1-15

    • DOI

      10.3389/fimmu.2019.01180

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exogenous application of L‐histidine suppresses bacterial diseases and enhances ethylene production in rice seedlings2019

    • Author(s)
      Yariyama S.、Ando S.、Seo S.、Nakaho K.、Miyashita S.、Kanayama Y.、Takahashi H.
    • Journal Title

      Plant Pathology

      Volume: 68 Issue: 6 Pages: 1072-1078

    • DOI

      10.1111/ppa.13037

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of the Immunomodulatory Activities of the Probiotic Strain Lactobacillus fermentum UCO-979C2019

    • Author(s)
      Garcia-Castillo Valeria、Komatsu Ryoya、Clua Patricia、Indo Yuhki、Takagi Michihiro、Salva Susana、Islam Md. Aminul、Alvarez Susana、Takahashi Hideki、Garcia-Cancino Apolinaria、Kitazawa Haruki、Villena Julio
    • Journal Title

      Frontiers in Immunology

      Volume: 10 Pages: 1376-1376

    • DOI

      10.3389/fimmu.2019.01376

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plant defense systems against cucumber mosaic virus: lessons learned from CMV-Arabidopsis interactions2019

    • Author(s)
      Sugihiro Ando ・ Shuhei Miyashita ・ Hideki Takahashi
    • Journal Title

      Journal of General Plant Pathology

      Volume: 85 Issue: 3 Pages: 174-181

    • DOI

      10.1007/s10327-019-00845-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] イネ苗細菌病抑制活性を持つ落葉堆肥から単離されたバクテリオファージの性状解析とその農業利用に向けた検討2024

    • Author(s)
      金井塚文音, 佐々木稜太, 宮下脩平, 高橋英樹
    • Organizer
      第76回北日本病害虫研究会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 青枯病菌に感染するジャンボファージの病害抑制効果の検討2023

    • Author(s)
      阿部香奈, 佐々木稜太, 金井塚文音, 宮下脩平, 安藤杉尋, 高橋英樹
    • Organizer
      令和5年度日本植物病理学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Ralstonia属細菌に感染するジャンボファージ属幅条件最適化の数理モデルを利用した検討2023

    • Author(s)
      宮下脩平, 高橋英樹
    • Organizer
      令和5年度日本植物病理学会東北部会
    • Related Report
      2023 Annual Research Report
  • [Presentation] イネもみ枯細菌病菌に感染する新奇 Siphoviridae科ファージの性状解析2022

    • Author(s)
      阿部香奈, 佐々木稜太, 金井塚綾音, 宮下脩平, 安藤杉尋, 高橋英樹
    • Organizer
      令和4年度日本植物病理学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Ralstonia属細菌に感染するファージ群の落葉堆肥からの検出とその解析2022

    • Author(s)
      佐々木稜太, 宮下脩平, 安藤杉尋, 高橋英樹
    • Organizer
      令和4年度日本植物病理学会東北部会
    • Related Report
      2022 Research-status Report
  • [Presentation] ナス科植物青枯病菌の保有するプロファージの誘発条件の検討と溶菌化プロファージの性状解析2022

    • Author(s)
      阿部香奈, 佐々木稜太, 宮下修平, 高橋英樹
    • Organizer
      令和4年度日本植物病理学会東北部会
    • Related Report
      2022 Research-status Report
  • [Presentation] Burkholderia 属細菌に感染する新奇ファージの遺伝子構造解析と病害防除利用への検討2021

    • Author(s)
      金井塚文音・佐々木稜太・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      令和3年度日本植物病理学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] イネもみ枯細菌病菌に感染するバクテリオファージの病害抑制効果および保存法の検討2021

    • Author(s)
      金井塚文音・佐々木稜太・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      令和3年度日本植物病理学会東北部会
    • Related Report
      2021 Research-status Report
  • [Presentation] 有機栽培育苗土からのイネもみ枯細菌病菌に感染するファージの探索と特性解析2019

    • Author(s)
      佐々木稜太・三富直人・宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      令和元年度日本植物病理学会東北部会
    • Related Report
      2019 Research-status Report
  • [Presentation] 有機栽培育苗土から作出したイネもみ枯細菌病抑制効果を持つ細菌集団の施用によるイネ内生菌叢への影響2019

    • Author(s)
      三富直人・宮下脩平・高橋英樹・安藤杉尋
    • Organizer
      令和元年度日本植物病理学会東北部会
    • Related Report
      2019 Research-status Report

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Published: 2019-07-04   Modified: 2025-01-30  

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