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Exhaustive search to identify plant peptides promoting synbiotic effects

Research Project

Project/Area Number 19K22313
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionKyushu Institute of Technology

Principal Investigator

Hanada Kousuke  九州工業大学, 大学院情報工学研究院, 教授 (50462718)

Co-Investigator(Kenkyū-buntansha) 柘植 尚志  中部大学, 応用生物学部, 教授 (30192644)
Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsシロイヌナズナ / 共生 / 短い遺伝子 / 共生菌 / Colletotrichum / ペプチド
Outline of Research at the Start

本研究では、植物のモデル生物であるシロイヌナズナと共生菌であるColletotrichum tofieldiae(Ct菌)を利用して、共生関係を促進させる植物由来のペプチド性遺伝子の同定を目指す。そこで、先行研究で構築している短い遺伝子のシロイヌナズナの過剰発現体にCt菌を感染させ、Ct菌の共生を促進させるペプチド性遺伝子を探索する。

Outline of Final Research Achievements

Since symbiosis requires a balance of growth with each other, it is considered that plants have a molecular mechanism that transmits to symbiotic fungi whether they need symbiotic fungi or not. Here, we found a peptide that artificially constructs a peptide secreted extracellularly and changes the morphology of symbiotic filamentous fungi. In order to clarify whether this indicates symbiotic induction of symbiotic fungus, we constructed a transformant that overexpresses the gene encoding the candidate peptide and a transformant that suppresses the gene expression, and infects Ct bacteria. The growth rates of wild-type and transformants were compared. As a result, it was found that the transformants overexpressing the peptide tended to promote the growth more than the wild type.

Academic Significance and Societal Importance of the Research Achievements

植物自身が、共生菌を増加してほしい状況か、細胞を抑制してほしい状況か、を伝達する分子メカニズムが存在するはずである。分子メカニズムを明らかにできた際には、植物共生菌を人為的にコントロールした農法に繋がる可能性がある。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (6 results)

All 2021 2020 2019

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results)

  • [Journal Article] Positive selective sweeps of epigenetic mutations regulating specialized metabolites in plants2021

    • Author(s)
      Shirai Kazumasa、Sato Mitsuhiko P.、Nishi Ranko、Seki Masahide、Suzuki Yutaka、Hanada Kousuke
    • Journal Title

      Genome Research

      Volume: 31 Issue: 6 Pages: 1060-1068

    • DOI

      10.1101/gr.271726.120

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Identification of endogenous small peptides involved in rice immunity through transcriptomics‐ and proteomics‐based screening2020

    • Author(s)
      Wang Pingyu、Yao Shaolun、Kosami Ken‐ichi、Guo Ting、Li Jing、Zhang Yuanyuan、Fukao Yoichiro、Kaneko‐Kawano Takako、Zhang Heng、She Yi‐Min、Wang Pengcheng、Xing Weiman、Hanada Kousuke、Liu Renyi、Kawano Yoji
    • Journal Title

      Plant Biotechnology Journal

      Volume: 18 Issue: 2 Pages: 415-428

    • DOI

      10.1111/pbi.13208

    • NAID

      120006812131

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Degree of Functional Divergence in Duplicates Is Associated with Distinct Roles in Plant Evolution2020

    • Author(s)
      Ezoe Akihiro、Shirai Kazumasa、Hanada Kousuke
    • Journal Title

      Molecular Biology and Evolution

      Volume: 38 Issue: 4 Pages: 1447-1459

    • DOI

      10.1093/molbev/msaa302

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of small coding genes on the circadian rhythms under elevated CO2 conditions in plants2020

    • Author(s)
      Higuchi-Takeuchi Mieko、Kondo Takayuki、Shimizu Minami、Kim You-Wang、Shinozaki Kazuo、Hanada Kousuke
    • Journal Title

      Plant Molecular Biology

      Volume: 104 Issue: 1-2 Pages: 55-65

    • DOI

      10.1007/s11103-020-01023-w

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hormone-like peptides and small coding genes in plant stress signaling and development2019

    • Author(s)
      FuminoriTakahashi, KousukeHanada, TakayukiKondo, KazuoShinozaki
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 51 Pages: 88-95

    • DOI

      10.1016/j.pbi.2019.05.011

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Contribution of Functional Divergence through Copy Number Variations to the Inter-species and Intra-species Diversity in Specialized Metabolites2019

    • Author(s)
      Kazumasa Shirai, Kousuke Hanada
    • Journal Title

      Frontiers in Plant Science

      Volume: 10 Pages: 1567-1567

    • DOI

      10.3389/fpls.2019.01567

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2019-07-04   Modified: 2023-01-30  

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