Exhaustive search to identify plant peptides promoting synbiotic effects
| Project/Area Number |
19K22313
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
Hanada Kousuke 九州工業大学, 大学院情報工学研究院, 教授 (50462718)
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| Co-Investigator(Kenkyū-buntansha) |
柘植 尚志 中部大学, 応用生物学部, 教授 (30192644)
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| Project Period (FY) |
2019-06-28 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シロイヌナズナ / 共生 / 短い遺伝子 / 共生菌 / Colletotrichum / ペプチド |
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| Outline of Research at the Start |
本研究では、植物のモデル生物であるシロイヌナズナと共生菌であるColletotrichum tofieldiae（Ct菌）を利用して、共生関係を促進させる植物由来のペプチド性遺伝子の同定を目指す。そこで、先行研究で構築している短い遺伝子のシロイヌナズナの過剰発現体にCt菌を感染させ、Ct菌の共生を促進させるペプチド性遺伝子を探索する。
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| Outline of Final Research Achievements |
Since symbiosis requires a balance of growth with each other, it is considered that plants have a molecular mechanism that transmits to symbiotic fungi whether they need symbiotic fungi or not. Here, we found a peptide that artificially constructs a peptide secreted extracellularly and changes the morphology of symbiotic filamentous fungi. In order to clarify whether this indicates symbiotic induction of symbiotic fungus, we constructed a transformant that overexpresses the gene encoding the candidate peptide and a transformant that suppresses the gene expression, and infects Ct bacteria. The growth rates of wild-type and transformants were compared. As a result, it was found that the transformants overexpressing the peptide tended to promote the growth more than the wild type.
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| Academic Significance and Societal Importance of the Research Achievements |
植物自身が、共生菌を増加してほしい状況か、細胞を抑制してほしい状況か、を伝達する分子メカニズムが存在するはずである。分子メカニズムを明らかにできた際には、植物共生菌を人為的にコントロールした農法に繋がる可能性がある。
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Report
(4 results)
Research Products
(6 results)
