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Host adaptation and niche expansion of pathogenic bacteria: a new approach of microbial ecology based on molecular evolution of colonization factors

Research Project

Project/Area Number 19K22459
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionOsaka City University (2020-2021)
Nagasaki University (2019)

Principal Investigator

WADA Takayuki  大阪市立大学, 大学院生活科学研究科, 教授 (70332450)

Co-Investigator(Kenkyū-buntansha) 西川 禎一  帝塚山学院大学, 人間科学部, 特任教授 (60183539)
Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords腸内細菌 / 宿主適応 / 定着因子 / 分子進化 / 細菌ゲノム
Outline of Research at the Start

定着因子は細菌表面に局在するタンパク質であり,宿主細胞と特異的に接着する機能を持つ.中でも,F4因子は様々な宿主から広く見出されている点で特徴的である.本課題では,F4因子の多様性と宿主適応の関連性に着目し,様々な生物種から同因子を保有する菌を網羅的に探索する.それらのDNA配列から分子進化学的検証を行い,宿主の壁を越えて感染を拡げる病原体のしたたかな生存戦略を解明する.病原細菌学的には,人獣共通感染症としての潜在的リスクを提唱し,医学・獣医学へのフィードバックの足がかりとする.こうした研究展開を微生物生態学と病原細菌学の双方にインパクトを与えうる「病原細菌の適応進化学」として確立する.

Outline of Final Research Achievements

In bacterial infection, colonization factors (CFs) on the surface of the bacteria need to recognize and adhere to host cells. Thus, the evolution of CFs can be regarded as a survival strategy for bacteria that spread infection over different hosts. In this project, we focused on a CF (κ-class CU) that is considered to have a high potential to switch the host range. We analyzed the sequence data of the genes of Esherichia coli strains isolated from various resources, established a detection system for the CF homologues, and successfully identified novel CFs possessing human cell adhesion properties.

Academic Significance and Societal Importance of the Research Achievements

微生物が宿主に感染する時,第1段階としてその宿主細胞に接着する必要がある.本研究では宿主細胞に接着するための菌体成分(定着因子)に着目し,ヒトや家畜,その他動物から分離された大腸菌株の定着遺伝子を比較・検証し,その相関や接着性能,新しいヒト接着因子の発見に結びつけた.こうした結果は,未知の食中毒・感染症の原因究明に役立てられる基礎的データとなるとともに,様々な動物を宿主として広がる感染性微生物の生態を知る手がかりとして活用していくことができる.

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (6 results)

  • [Int'l Joint Research] 国立屏東科技大学(台湾)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] 国立屏東科技大学(台湾)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli2022

    • Author(s)
      1.Tanimoto Y, Inoue M, Komatsu K, Odani A, Wada T, Kage-Nakadai E, Nishikawa Y.
    • Journal Title

      Epidemiology and Infection

      Volume: 150 Pages: 1-5

    • DOI

      10.1017/s0950268821002697

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonization factor detected in human enterotoxigenic Escherichia coli2021

    • Author(s)
      Yoshihiko Tanimoto, Miyoko Inoue, Kana Komatsu, Atsuyuki Odani, Takayuki Wada, Eriko Kage-Nakadai, Yoshikazu Nishikawa
    • Journal Title

      bioRxiv

      Volume: 2021.05.01 Pages: 442292-442292

    • DOI

      10.1101/2021.05.01.442292

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Journal Article] Prevalence of Virulence Genes of Diarrheagenic <i>Escherichia coli</i> in Fecal Samples Obtained from Cattle, Poultry and Diarrheic Patients in Bangladesh2020

    • Author(s)
      Parvej MS, Alam MA, Shono M, Zahan MN, Masuma Parvez MM, Ansari WK, Jowel MS, Uddin MS, Kage-Nakadai E, Rahman MT, Nishikawa Y
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 73 Issue: 1 Pages: 76-82

    • DOI

      10.7883/yoken.JJID.2019.016

    • NAID

      130007789154

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Host range-associated clustering based on multi-locus variable-number tandem-repeat analysis, phylotypes, and virulence genes of atypical enteropathogenic Escherichia coli strains2019

    • Author(s)
      Parvej MS, Nakamura H, Alam MA, Wang L, Zhang S, Emura K, Kage-Nakadai E, Wada T, Hara-Kudo Y, Nishikawa Y.
    • Journal Title

      Appl Environ Microbiol.

      Volume: 85 Issue: 6 Pages: e02796-18

    • DOI

      10.1128/aem.02796-18

    • NAID

      120006727806

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019617

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Distribution of Carnivore protoparvovirus 1 in free-living leopard cats (Prionailurus bengalensis chinensis) and its association with domestic carnivores in Taiwan2019

    • Author(s)
      Chen CC, Chang AM, Wada T, Chen MT, Tu YS
    • Journal Title

      PLoS ONE

      Volume: 14(9) Issue: 9 Pages: e0221990-e0221990

    • DOI

      10.1371/journal.pone.0221990

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of loop-mediated isothermal amplification for rapid and convenient detection of Mycobacterium marinum complex2019

    • Author(s)
      Tsai MA, Wang PC, Yoshida S, Aono A, Mitarai S, Wada T, Chen SC
    • Journal Title

      Journal of Microbiological Methods

      Volume: 164 Pages: 105671-105671

    • DOI

      10.1016/j.mimet.2019.105671

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 定型接着因子不明な分散接着性大腸菌の全ゲノム解析2022

    • Author(s)
      山﨑康祐,谷本佳彦,陳彦霖,西川禎一,中台(鹿毛)枝里子,和田崇之
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Annual Research Report
  • [Presentation] astA保有大腸菌におけるF4相同性接着因子の検出2022

    • Author(s)
      鈴木優希,谷本佳彦,陳彦霖,西川禎一,和田崇之
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 定型接着因子不明な分散接着性大腸菌の全ゲノム解析2021

    • Author(s)
      山﨑康祐,谷本佳彦,陳彦霖,西川禎一,中台(鹿毛)枝里子,和田崇之
    • Organizer
      第42回日本食品微生物学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] astA保有大腸菌におけるF4相同性接着因子の検出2021

    • Author(s)
      鈴木優希,谷本佳彦,陳彦霖,西川禎一,和田崇之
    • Organizer
      第42回日本食品微生物学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ブタおよびウシにおけるヒト腸管毒素原性大腸菌O169:H41の新規付着因子に対する抗体保有状況2020

    • Author(s)
      井上三代子,小松加奈,鄭冬明,大森裕子,山口良弘,宮田真人,和田崇之,中台(鹿毛)枝里子,西川禎一
    • Organizer
      第93回日本細菌学会総会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒト腸管毒素原性大腸菌O169:H41の新規定着因子に対する抗体のブタにおける保有状況2019

    • Author(s)
      井上三代子,小松加奈,鄭冬明,大森裕子,山口良弘,宮田真人,和田崇之,中台(鹿毛)枝里子,西川禎一
    • Organizer
      第72回日本細菌学会関西支部総会
    • Related Report
      2019 Research-status Report

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Published: 2019-07-04   Modified: 2023-01-30  

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