Dynamics of microenvironmental regulation by extracellular vesicles with carbohydrates and molecular cluster formation

• Project/Area Number: 19K22518
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 48:Biomedical structure and function and related fields
• Research Institution: Chubu University
• Principal Investigator: Furukawa Koichi 中部大学, 生命健康科学部, 特任教授 (80211530)
• Co-Investigator(Kenkyū-buntansha): 大海 雄介 中部大学, 生命健康科学部, 助教 (10584758) ; 古川 圭子 中部大学, 生命健康科学部, 教授 (50260732)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 細胞外分泌小胞 / 微小環境 / エクソソーム / サブセット / クラスター形成 / シグナル / 細胞外小胞 / 糖脂質 / ガングリオシド / クラスター

Outline of Research at the Start

癌細胞、免疫細胞等の制御における細胞外分泌小胞（エクソソーム）の役割が注目されているが、その実体は不明点が多く、複合糖鎖に関する知見は乏しい。スフィンゴ糖脂質がエクソソームで膜分子と複合体を形成して働くことを想定し、1.糖鎖に依拠したエクソソームの分離・分取法開発と機能的サブセットの解析、2.エクソソームの細胞取込みでの糖鎖複合体サブセットの分別的機能や作用機構の解明、3.エクソソーム搭載分子の人為的改変法を開発し、その情報伝達プローブとしての応用と病態制御法の開発、を目指す。

Outline of Final Research Achievements

We investigated regulatory function of extracellular vesicles (EV, exosome) released from various cancer cells for cancer microenvironments with a focus on the expression of cancer-associated acidic glycolipids on EVs. At first, we established methods for the isolation and purification of EVs based on the expressed carbohydrates using Tim4-beads. Then, we compared chemical composition of lipids on EVs and cell membrane, and clarified similarity between them. Furthermore, we demonstrated possible cluster formation of membrane molecules and glycolipids on EVs. We also established optimal culture conditions for the separation and purification of EV samples. Finally, we showed functions of EVs released from cancer-associated sugar-expressing cells to induce various malignant properties and signal activation towards other cells.

Academic Significance and Societal Importance of the Research Achievements

癌細胞から分泌されるEVの機能が多く報告されているが、最も注目されるのは、その転移先の決定機能と転移ニッチの誘導機能である。要するに癌転移におけるEVの重要性が想定される。これまで、癌関連糖脂質が細胞膜上で接着受容体、インテグリン等と複合体を形成して癌形質を増強することを報告してきたが、本研究で同様の共同作用がEV上で発揮されることが分かってきた。
したがって、癌関連糖鎖を搭載するEV上で、糖鎖が膜分子とクラスターを形成して協働することを明らかにすることは、EVの本体を解明する上で学術的な意義は極めて大きい、さらに、その制御技術の開発により癌転移の予防、治療に大きく貢献することが期待される。

Research Products

• [Int'l Joint Research] Cittagong University/Dep Biochem and Mol Biol(バングラデシュ)
• [Int'l Joint Research] Harvard University/Dep Neurol, Boston Children’s Hosp(米国)
• [Int'l Joint Research] Chittagong University(バングラデシュ)
• [Journal Article] Distinguishing Functional Exosomes and Other DNA-containing Extracellular Vesicles Based on the Difference in Strength of Negative Membrane Charge (2022)
  • Author(s): Seo, Naohiro; Nakamura, Junko; Kaneda, Tsuguhiro; Tateno, Hiroaki; Shimoda, Asako; Ichiki, Takanori; Furukawa, Koichi; Hirabayashi, Jun; Kazunari, Akiyoshi; Shiku, Hiroshi
  • Journal Title: J. Extracell. Vesicles Volume: 11 Issue: 3 Pages: 12205-12205
  • DOI: 10.1002/jev2.12205
  • Peer Reviewed / Open Access
• [Journal Article] Signaling domains of cancer-associated glycolipids. Tribute to Professor Sen-itiroh Hakomori (2022)
  • Author(s): Furukawa, K., Ohmi, Y., Hamamura, K., Kondo, Y., Ohkawa, Y., Kaneko, K., Hashimoto, N., Farhana, Y., Bhuiyan, R.H., Tajima, O., Furukawa, K.
  • Journal Title: Glycoconj. J. Volume: 39 Issue: 2 Pages: 145-155
  • DOI: 10.1007/s10719-022-10051-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling (2021)
  • Author(s): Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K
  • Journal Title: Anticancer Res. Volume: 41 Issue: 4 Pages: 1821-1830
  • DOI: 10.21873/anticanres.14948
  • Peer Reviewed / Open Access
• [Journal Article] Lack of GD3 synthase (St8sia1) attenuates malignant properties of gliomas in genetically engineered mouse model. (2021)
  • Author(s): Ohkawa, Y., Zhang, P., Momota, H., Kato, A., Hashimoto, N., Ohmi, Y., Bhuiyan, R.H., Natsume, A., Wakabayashi, T., Furukawa, K, Furukawa K.
  • Journal Title: Cancer Sci. Volume: 112 Issue: 9 Pages: 3756-3768
  • DOI: 10.1111/cas.15032
  • Peer Reviewed / Open Access
• [Journal Article] St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity (2021)
  • Author(s): Zhang, P., Ohkawa, Y., Yamamoto, S., Momota, H., Kato, A., Kaneko, K., Natsume, A., Farhana, Y., Ohmi, Y., Okajima, T., Bhuiyan, R.H., Wakabayashi, T., Furukawa, K., Furukawa, K.
  • Journal Title: Nagoya Journal of Medical Science Volume: 83 Issue: 3 Pages: 535-549
  • DOI: 10.18999/nagjms.83.3.535
  • NAID: 120007147350
  • ISSN: 0027-7622
  • URL: https://nagoya.repo.nii.ac.jp/records/2001444
  • Peer Reviewed / Open Access
• [Journal Article] Contribution of glucosylceramide synthase to the proliferation of mouse osteoblasts (2021)
  • Author(s): Mishima, Y., Hamamura, K., Kato, H., Furukawa, K., Tashima, Y., Okajima, T., Kondo, H., Sato, T., Miyazawa, K., Goto, S., Togari, A.
  • Journal Title: In Vivo Volume: 35 Issue: 6 Pages: 3111-3123
  • DOI: 10.21873/invivo.12606
  • Peer Reviewed / Open Access
• [Journal Article] Ganglioside GD2 Enhances the Malignant Phenotypes of Melanoma Cells by Cooperating with Integrins. (2021)
  • Author(s): Yesmin, F., Bhuiyan, R.H., Ohmi, Y., Yamamoto, S., Kaneko, K., Ohkawa, Y., Zhang, P., Hamamura, K., Cheung, N-K.V., Kotani, N., Honke, K., Okajima, T., Kambe, M., Tajima, O., Furukawa, K., Furukawa, K.
  • Journal Title: Int. J. Mol. Sci. Volume: 23 Issue: 1 Pages: 423-442
  • DOI: 10.3390/ijms23010423
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A nontoxigenic form of Shiga toxin 2 suppresses the production of amyloid b by altering the intracellular transport of amyloid precursor protein through its receptor-binding B-subunit (2021)
  • Author(s): Waka Sato, Miho Watanabe-Takahashi, Takashi Hamabata, Koichi Furukawa, Satoru Funamoto, Kiyotaka Nishikawa
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 557 Pages: 247-253
  • DOI: 10.1016/j.bbrc.2021.04.015
  • Peer Reviewed / Open Access
• [Journal Article] St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity. (2021)
  • Author(s): Zhang P, Ohkawa Y, Yamamoto S, Momota H, Kato A, Kaneko K, Natsume A, Farhana Y, Ohmi Y, Okajima T, Bhuiyan R.H., Wakabayashi T, Furukawa K, Furukawa K
  • Journal Title: Nagoya J. Med. Sci. Volume: in press
  • NAID: 120007147350
  • Peer Reviewed / Open Access
• [Journal Article] Majority of alpha2,6-sialylated glycans in adult mouse brain exist in O-glycans: SALSA-MS analysis for knockout mice of alpha2,6-sialyltransferase genes (2020)
  • Author(s): Ohmi Y, Nishikaze T, Kitaura Y, Ito T, Yamamoto S, Sugiyama F, Matsuyama M, Takahashi Y, Takeda A, Kawahara T, Okajima T, Furukawa K, Furukawa K
  • Journal Title: Glycobiology Volume: 105 Issue: 5 Pages: 1-14
  • DOI: 10.1093/glycob/cwaa105
  • Peer Reviewed / Open Access
• [Journal Article] Furukawa K. (2020)
  • Author(s): Furukawa K.
  • Journal Title: Int J Mol Sci. Volume: 21 Issue: 18 Pages: 6552-6552
  • DOI: 10.3390/ijms21186552
  • Peer Reviewed / Open Access
• [Journal Article] Aminoglycosides are efficient reagents to induce readthrough of premature termination codon in mutant B4GALNT1 genes found in families of hereditary spastic paraplegia (2020)
  • Author(s): Yesmin F, Bhuiyan RH, Ohmi Y, Ohkawa Y, Tajima O, Okajima T, Furukawa K, Furukawa K.
  • Journal Title: J Biochem. Volume: 168 Issue: 2 Pages: 103-112
  • DOI: 10.1093/jb/mvaa041
  • NAID: 40022308727
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SSEA-3 and 4 are not essential for the induction or properties of mouse iPS cells (2020)
  • Author(s): Hamamura Kazunori、Hotta Hiroshi、Murakumo Yoshiki、Shibuya Hidenobu、Kondo Yuji、Furukawa Koichi
  • Journal Title: Journal of Oral Science Volume: 62 Issue: 4 Pages: 393-396
  • DOI: 10.2334/josnusd.19-0513
  • NAID: 130007908552
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed / Open Access
• [Journal Article] Novel molecular mechanisms for roles of gangliosides in the nervous system elucidated by genetic engineering. (2020)
  • Author(s): Furukawa, K., Ohmi, Y., Yesmin, F., Tajima, O., Kondo, Y., Pu Zhang, P., Hashimoto, N., Ohkawa, Y., Bhuiyan, R.H., Furukawa, K.
  • Journal Title: Int. J. Mol. Sci. Volume: 21 Issue: 6 Pages: 1906-1906
  • DOI: 10.3390/ijms21061906
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Elucidation of Pathological Mechanisms for Involvement of Gangliosides in Intractable Diseases. (2020)
  • Author(s): Ohmi Y, Furukawa K, Furukawa K
  • Journal Title: Trends Glycosci. Glycotechnol. Volume: 32
  • NAID: 130007846168
  • Peer Reviewed / Open Access
• [Journal Article] The ceramide moiety of disialoganglioside (GD3) is essential for GD3 recognition by the sialic acid-binding lectin SIGLEC7 on the cell surface. (2019)
  • Author(s): Hashimoto N, Ito S, Tsuchida A, Bhuiyan RH, Okajima T, Yamamoto A, Furukawa K, Ohmi Y, Furukawa K.
  • Journal Title: J Biol Chem. Volume: 294 Issue: 28 Pages: 10833-10845
  • DOI: 10.1074/jbc.ra118.007083
  • NAID: 120007168785
  • Peer Reviewed / Open Access
• [Journal Article] Cholera toxin B induces interleukin-1β production from resident peritoneal macrophages through the pyrin inflammasome as well as the NLRP3 inflammasome (2019)
  • Author(s): Orimo T, Sasaki I, Hemmi H, Ozasa T, Fukuda-Ohta Y, Ohta T, Morinaka M, Kitauchi M, Yamaguchi T, Sato Y, Tanaka T, Hoshino K, Katayama KI, Fukuda S, Miyake K, Yamamoto M, Satoh T, Furukawa K, Kuroda E, Ishii KJ, Takeda K, Kaisho T
  • Journal Title: International Immunology Volume: 印刷中 Issue: 10 Pages: 657-668
  • DOI: 10.1093/intimm/dxz004
  • Peer Reviewed / Open Access
• [Journal Article] Deficiency of GD3 Synthase in Mice Resulting in the Attenuation of Bone Loss with Aging (2019)
  • Author(s): Yo S, Hamamura K, Mishima Y, Hamajima K, Mori H, Furukawa K, Kondo H, Tanaka K, Sato T, Miyazawa K, Goto S, Togari A
  • Journal Title: Int. J. Mol. Sci. Volume: 10 Issue: 11 Pages: 2825-2825
  • DOI: 10.3390/ijms20112825
  • Peer Reviewed / Open Access
• [Journal Article] TNFα-signal and cAMP-mediated signals oppositely regulate melanoma- associated ganglioside GD3 synthase gene in human melanocytes. (2019)
  • Author(s): Takeuchi R, Kambe M, Miyata M, Jeyadevan U, Tajima O, Furukawa K, Furukawa K.
  • Journal Title: Sci Rep. Volume: 9 Issue: 1 Pages: 14740-14740
  • DOI: 10.1038/s41598-019-51333-3
  • Peer Reviewed / Open Access
• [Journal Article] Loss of enzyme activity of mutated B4GALNT1 gene products found in patients with hereditary spastic paraplegia induces relatively mild neurological disorders: Similarity with phenotypes of B4galnt1 knockout mice. (2018)
  • Author(s): Robiul H. Bhuiyan, Yuhsuke Ohmi, Yuki Ohkawa, Pu Zhang, Maiko Takano, Noboru Hashimoto, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Journal Title: Neuroscience Volume: 397 Pages: 94-106
  • DOI: 10.1016/j.neuroscience.2018.11.034
  • Peer Reviewed
• [Presentation] Analysis of control mechanisms of cancer microenvironments by sugar chains in colorectal cancer cells (2021)
  • Author(s): Momoka Horiuchi, Yusuke Omi, Satoko Yamamoto, Hiroaki Kitazawa, Farhana Yesmin, Robiul Bhuiyan, Orie Tajima, Keiko Furukawa, Noboru Hashimoto, Koichi Furukawa
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Changes of integrins in glycolipid-modified melanoma-derived exosomes (2021)
  • Author(s): Kei Kaneko, Yuhsuke Ohmi, Mariko Kambe, Qi Li, Yesmin Farhana, Satoko Yamamoto, Yoko Kitaura, Takako Ito, Robiul H. Bhuiyan, Orie Tajima, Koichi Furukawa, Keiko Furukawa
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2021)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Momoka Horiuchi, Tetsuya Okajima, Satoko Yamamoto, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Unique effects of expression of asialo-series ganglioside GD1alpha in human cancer cell lines (2021)
  • Author(s): Bhuiyan Robiul H., Farhana Yesmin, Yuji Kondo, Yuki Ohkawa, Yuhsuke Ohmi, Pu Zhang, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Development of novel and fully-murinized assessment system for CAR-T therapy (2021)
  • Author(s): 根岸航平、藤原 弘、赤堀 泰、三輪啓志、王 立楠、古川鋼一、珠玖 洋
  • Organizer: 第80回日本癌学会学術総会
• [Presentation] Ganglioside GD2 cooperate with integrin in the enhancement of malignant properties of melanoma cells (2021)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Momoka Horiuchi, Tetsuya Okajima, Satoko Yamamoto, Kei Kaneko, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第94回日本生化学会大会
• [Presentation] スフィンゴ糖脂質による肝脂肪蓄積制御メカニズムの検討 (2021)
  • Author(s): 田島織絵、古川圭子、古川鋼一
  • Organizer: 第94回日本生化学会大会
• [Presentation] ヒトグリオーマ細胞由来エクソソームに発現するガングリオシドの機能解析 (2021)
  • Author(s): 大海雄介、Li Qi、山本聡子、北浦洋子、伊藤多佳子、佐藤詩歩、金子 慶、神戸眞理子、イエスミン ファーハナ、田島織絵、モハンマド A ハスナット、河原敏男、古川圭子、古川鋼一
  • Organizer: 第94回日本生化学会大会
• [Presentation] 初代培養アストロサイト上に発現するガングリオシドの役割 (2021)
  • Author(s): 佐藤詩歩、北浦洋子、伊藤多佳子、田島織絵、古川圭子、河原敏男、古川鋼一、大海雄介
  • Organizer: 第94回日本生化学会大会
• [Presentation] Ganglioside GD3 enhances the expression of integrins on extracellular vesicles (EVs) to exert its functions (2021)
  • Author(s): Kei Kaneko, Yuhsuke Ohmi, Mariko Kambe, Farhana Yesmin, Satoko Yamamoto, Yoko Kitaura, Takako Ito, Orie Tajima, Koichi Furukawa, Keiko Furukawa
  • Organizer: 第40回日本糖質学会年会
• [Presentation] アストロサイトに発現するガングリオシドの機能解析と近傍分子の同定 (2021)
  • Author(s): 佐藤詩歩、北浦洋子、伊藤多佳子、田島織絵、小谷典弘、本家孝一、古川圭子、河原敏男、古川鋼一、大海雄介
  • Organizer: 第40回日本糖質学会年会
• [Presentation] がん由来エクソソーム上に発現するガングリオシドのがん微小環境への影響 (2021)
  • Author(s): 大海雄介、Li Qi、山本聡子、北浦洋子、伊藤多佳子、佐藤詩歩、金子 慶、神戸眞理子、イエスミン ファーハナ、田島織絵、モハンマド A ハスナット、古川圭子、古川鋼一
  • Organizer: 第40回日本糖質学会年会
• [Presentation] ヒト大腸癌細胞による糖脂質発現と機能の解析 (2021)
  • Author(s): 堀内萌々花、橋本登、大海雄介、山本聡子、北沢裕昭、Yesmin Farhana、田島織絵、古川圭子、古川鋼一
  • Organizer: 第40回日本糖質学会年会
• [Presentation] Elucidation of unique function of ganglioside GD2 and its action mechanisms in human melanoma (2021)
  • Author(s): Yesmin F, Robiul H. Bhuiyan、Yuhsuke Ohmi、 Momoka Horiuchi、Tetsuya Okajima、Satoko Yamamoto、Kei Kaneko、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第40回日本糖質学会年会
• [Presentation] Different expression of integrins in ganglioside-remodeling melanoma EVs (2021)
  • Author(s): Kei Kaneko, Yuhsuke Ohmi, Mariko Kambe, Yesmin Farhana, Momoka Horiuchi, Yoko Kitaura, Takako Ito, Orie Tajima, Koichi Furukawa and Keiko Furukawa
  • Organizer: 10th国際EV学会
  • Int'l Joint Research
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2021)
  • Author(s): Farhana Yesmin, Robiul H. Bhuiyan, Yuhsuke Ohmi, Momoka Horiuchi, Tetsuya Okajima, , Kei Kaneko, Keiko Furukawa, Koichi Furukawa
  • Organizer: 10th国際EV学会
  • Int'l Joint Research
• [Presentation] GD3/GD2-expressing glioma-derived EVs enhance malignant properties of gliomas, and regulate tumor microenvironments (2021)
  • Author(s): Koichi Furukawa, Yuhsuke Ohmi, Qi Li, Hiroaki Kitasawa, Yesmin Farhana, Satoko Yamamoto, Yoko Kitaura, Takako Ito, Kei Kaneko, Mariko Kambe, Keiko Furukawa
  • Organizer: 10th国際EV学会
  • Int'l Joint Research
• [Presentation] ヒトグリオーマ細胞由来エクソソームの機能解析 (2021)
  • Author(s): 大海雄介、Li Qi、山本聡子、北浦洋子、伊藤多佳子、金子 慶、神戸眞理子、イエスミン ファーハナ、田島織絵、モハンマド A ハスナット、古川圭子、古川鋼一
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] 糖脂質リモデリング腫瘍細胞由来細胞外小胞の機能解析 (2021)
  • Author(s): LIU QINYUE, 澤田晋一, 佐々木善浩, 古川鋼一, 秋吉一成
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] ガングリオシド改変メラノーマ細胞由来EVsにおけるintegrin の発現パターンとがん形質機能の解析 (2021)
  • Author(s): 金子 慶、大海雄介、神戸真理子、ファーハナ イエスミン、山本聡子、北浦洋子、伊藤多佳子、田島織絵、古川鋼一、古川圭子
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin (2021)
  • Author(s): Farhana Yesmin、Robiul H. Bhuiyan、Yuhsuke Ohmi、Momoka Horiuchi、Tetsuya Okajima、Satoko Yamamoto、Kei Kaneko、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] がんの進行阻害作用を持つCTLエクソソームと その作用を持たない CTL EVの生理学的性質の検討 (2021)
  • Author(s): 瀬尾尚宏、中村純子、金田次弘、舘野浩章、下田麻子、一木隆範、古川鋼一、平林淳、秋吉一成、珠玖洋
  • Organizer: 第8回日本細胞外小胞学会学術集会
• [Presentation] コレラ毒素による免疫アジュバント活性における小胞体ストレスセンサーIRE1αの機能的意義 (2021)
  • Author(s): 佐々木泉、中井千尋、大田有里、西山奈央子、森田修平、奥崎大介、三並桃佳、林茉里、張江伊水、田中日向子、加藤喬、折茂貴是、古川鋼一、改正恒康
  • Organizer: 第25回日本がん免疫学会総会
• [Presentation] Extracellular vesicles secreted from melanoma cell lines contain high levels of integrins and similar profiles of ganglioside species with parent cells. (2020)
  • Author(s): Koichi Furukawa, Yuya Senoo, Mariko Kambe, Kazutaka Ikeda, Yuhsuke Ohmi, Satoko Yamamoto, Iori Kobayashi, Yuki Ohkawa, Keiko Furukawa
  • Organizer: ISEV (国際細胞外小胞学会) 2020
  • Int'l Joint Research
• [Presentation] Ganglioside GD2 enhances malignant properties of melanoma by co-operating with integrin. (2020)
  • Author(s): Yesmin Farhana, Robiul H. Bhuiyan, Yuhsuke Ohmi, Tetsuya Okajima, Satoko Yamamoto, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第79回日本癌学会総会
• [Presentation] Roles of asialo-series ganglioside GD1alpha in human cancer cell lines. (2020)
  • Author(s): Robiul Hasan Bhuiyan, Farhana Yesmin, Yuji Kondo, Yuki Ohkawa, Yuhsuke Ohmi, Pu Zhang, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第79回日本癌学会総会
• [Presentation] スフィンゴ糖脂質による肝インスリンシグナル制御を介した脂肪肝抑制機構の検討 (2020)
  • Author(s): 田島織絵、古川圭子、古川鋼一
  • Organizer: 第93回日本生化学会
• [Presentation] 初代培養アストロサイト上に発現するガングリオシドの機能解析 (2020)
  • Author(s): 佐藤詩歩、北浦洋子、伊藤多佳子、古川圭子、河原敏男、古川鋼一、大海雄介
  • Organizer: 第39回日本糖質学会年会
• [Presentation] 大腸癌細胞の糖鎖発現解析と糖鎖改変の試み：癌微小環境の制御機構の解析をめざして (2020)
  • Author(s): 堀内萌々花、大海雄介、山本聡子、北沢裕昭、Yesmin Farhana, 田島織絵、古川圭子、古川鋼一
  • Organizer: 第39回日本糖質学会年会
• [Presentation] The roles of unfold protein responses in cholera toxin B-induced interleukine-1b production. (2020)
  • Author(s): Izumi Sasaki, Takashi Orimo, Isui Harie, Yuko Takisawa, Koichi Furukawa, Tsuneyasu Kaisho
  • Organizer: 第27回日本免疫毒性学会学術年会
• [Presentation] The role of IRE1α in cholera toxin B-induced interleukine-1β production from resident peritoneal macrophages. (2020)
  • Author(s): Izumi Sasaki, Chihiro Nakai, Shuhei Morita, Daisuke Okuzaki, Yuri Fukuda-Ohta, Takashi Orimo, Hiroaki Hemmi, Koichi Furukawa, Tsuneyasu Kaisho
  • Organizer: マクロファージ分子細胞生物学研究会 (MMCB) 第27回マクロファージ分子細胞生物学国際シンポジウム
• [Presentation] Novel cellular immunotherapy using GD2-specific CAR gene-modified allogeneic γ/δ-T cells against solid cancers. (2020)
  • Author(s): Hiroshi Fujiwara, Yasushi Akahori, Hiroshi Miwa, Linan Wang, Chisaki Hyuga, Yusuke Ohmi, Yoshimasa Tanaka, Keiko Furukawa, Koichi Furukawa, Yoshihiro Miyahara, Hiroshi Shiku
  • Organizer: 第82回日本血液学会学術集会
• [Presentation] 新規GD2特異的CAR遺伝子導入アロγ/δ-T 細胞の開発 (2020)
  • Author(s): 藤原 弘, 赤堀泰, 三輪啓志, 王立楠,日向千咲, 大海雄介, 宮原慶裕, 田中義正, 古川圭子, 古川鋼一, 珠玖 洋
  • Organizer: 第24回日本癌免疫学会
• [Presentation] Regulation of cell signals by glycosphingolipids via cluster formation on the cell surface (2019)
  • Author(s): Koichi Furukawa, Yuki Ohkawa, Kei Kaneko, Noboru Hashimoto, Zhang Pu, Robiul H. Bhuiyan, Yuhsuke Ohmi, Keiko Furukawa
  • Organizer: International Symposium on Bio-CHAINs from Single Molecules to Highly Organized Systems. co-organized by G-CHAIN and CREST exosome project
  • Int'l Joint Research / Invited
• [Presentation] 糖鎖とシグナル伝達 (Sugar chains and signal transduction) (2019)
  • Author(s): 古川鋼一
  • Organizer: 第17回糖鎖科学コンソーシアムシンポジウム(JCGG) Symposium ~糖鎖研究と他領域との統合~
  • Invited
• [Presentation] Hydroxylation of ceramide modifies sensitivity of GD3-positive cancer cells to the killing of natural killer cells (2019)
  • Author(s): Koichi Furukawa, Noboru Hashimoto, Akihito Yamamoto, Paul Crocker, Yusuke Ohmi, Robiul H. Bhuiyan, Keiko Furukawa
  • Organizer: International Symposium on Glycoconjugates (IGO GLYCO25)
  • Int'l Joint Research / Invited
• [Presentation] シアル酸結合レクチンSiglec7のガングリオシドGD3認識に対するセラミド構造の影響 (2019)
  • Author(s): 橋本 登、伊藤静香、土田明子、Roubiul H. Bhuiyan、岡島徹也、山本朗仁、古川圭子、大海雄介、古川鋼一
  • Organizer: 第38回日本糖質学会
• [Presentation] 大腸癌におけるGb3の機能解析 (2019)
  • Author(s): 北沢裕昭、田中 祥、藤井 碧、大海雄介、大川祐樹、喬 善楼、田島織絵、古川圭子、古川鋼一
  • Organizer: 第38回日本糖質学会
• [Presentation] Unique function of ganglioside GD2 in malignant melanomas (2019)
  • Author(s): Farhana Yesmin、Robiul H. Bhuiyan、Yuhsuke Ohmi、Tetsuya Okajima、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第38回日本糖質学会
• [Presentation] Roles of ganglioside GD3 in the regulation of microenvironment of Gliomas (2019)
  • Author(s): Pu Zhang、Yuki Ohkawa、Robiul H. Bhuiyan、Hiroyuki Momota、Toshihiko Wakabayashi、Akira Kato、Yuhsuke Ohmi、Keiko Furukawa、 Tetsuya Okajima、Koichi Furukawa
  • Organizer: 第38回日本糖質学会
• [Presentation] Roles of ganglioside GD3 in the regulation of microenvironment of gliomas (2019)
  • Author(s): Pu Zhang、Okiru Komine、Yuki Ohkawa、Yuhsuke Ohmi、Robiul H. Bhuiyan、Akira Kato、Keiko Furukawa、Tetsuya Okajima、Toshihiko Wakabayashi、Koichi Furukawa
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] Targeting GD2 on cancer cells by an anti-GD2 antibody is promising in a variety of cancers: Challenge by CAR T (2019)
  • Author(s): 古川鋼一、赤堀 泰、大海雄介、ファーハナ イエスミン、浦野 健、古川圭子、珠玖 洋
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] TNFa-and cAMP-signals oppositely regulate melanoma associated ganglioside GD3 synthase gene in human melanocytes (2019)
  • Author(s): 竹内理香、神戸真理子、宮田麻衣子、ウプル ジャワデワン、田島織絵、古川鋼一、古川圭子
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] Development of new CAR that suppress GD2 expressing tumors (2019)
  • Author(s): 赤堀 泰、古川鋼一、藤原 弘、美輪啓志、奥村悟司、王立楠、加藤琢磨、宮原慶裕、瀬尾尚宏、 田中義正、珠玖 洋
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] Unique function of ganglioside GD2 in malignant melanomas (2019)
  • Author(s): Farhana Yesmin、Robiul H. Bhuiyan、Yuhsuke Ohmi、Tetsuya Okajima、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] Roles of asialo-series ganglioside GD1alpha in human cancer cell lines (2019)
  • Author(s): Robiul H. Bhuiyan、Farhana Yesmin、Yuji Kodo、Yuki Ohkawa、Yuhsuke Ohmi、Pu Zhang、Tetsuya Okajima、Keiko Furukawa、Koichi Furukawa
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] マウスアストロサイト上に発現するガングリオシドの機能解析 (2019)
  • Author(s): 大海雄介, 北浦洋子, 伊藤多佳子, 伴崚太, 古川圭子, 武田明, 河原敏男, 古川鋼一
  • Organizer: 第92回日本生化学会大会
• [Presentation] Increased readthrough of premature termination codon of B4GALNT1 gene depending on cell cycle (2019)
  • Author(s): Robiul H. Bhuiyan, Farhana Yesmin, Yuhsuke Ohmi, Yuki Ohkawa, Tetsuya Okajima, Keiko Furukawa, Koichi Furukawa
  • Organizer: 第92回日本生化学会大会
• [Presentation] Extracellular vesicles secreted from ganglioside GD3-expressing cancer cell lines contain high levels of integrins: Roles of lipid rafts (2019)
  • Author(s): Koichi Furukawa, Iori Kobayashi, Yoshiki Kodama, Yuhsuke Ohmi, Satoko Yamamoto, Yuki Ohkawa, Mariko Kambe, Keiko Furukawa
  • Organizer: ISEV (International Society for Extracellular Vesicles)
• [Book] 糖鎖生物学、生命現象と糖鎖情報 神経と糖鎖(II) 神経機能 (2020)
  • Author(s): 古川鋼一、大海雄介、田島織絵、古川圭子
  • Publisher: 名古屋大学出版会
• [Book] 糖鎖生物学、生命現象と糖鎖情報 免疫と糖鎖(I) 免疫調節 (2020)
  • Author(s): 大海雄介、古川鋼一
  • Publisher: 名古屋大学出版会
  • ISBN: 9784815809812
• [Remarks] Chubu Univ Coll Life Health Sci, Dep Glycobiol
  • URL: http://koichichubu.starfree.jp
• [Remarks] 中部大学 生命健康科学部 糖鎖生物学講座
  • URL: http://koichichubu.starfree.jp
• [Remarks] 癌関連糖鎖を標的にした新規癌治療法の開発
  • URL: https://www.chubu.ac.jp/about/faculty/profile/835a9495737bccf6f4128d87c4aabc80b41827bc.pdf
• [Patent(Industrial Property Rights)] GD2結合性分子 (2019)
  • Inventor(s): 珠玖洋、赤堀泰、三輪啓志、藤原弘、古川鋼一、古川圭子、大海雄介
  • Industrial Property Rights Holder: 三重大学、中部大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-142358
  • Filing Date: 2019