Establishment of PDOX mouse model for biliary tract cancer using super-microsurgery
| Project/Area Number |
19K22656
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 55:Surgery of the organs maintaining homeostasis and related fields
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| Research Institution | Kanazawa University (2020-2022)
Kyoto University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田浦 康二朗 京都大学, 医学研究科, 准教授 (80378629)
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| Project Period (FY) |
2019-06-28 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | PDOX / ヌードマウス / 胆管癌 / 胆道癌 / 患者由来腫瘍同所移植 / 個別化治療 / 患者由来同所性異種移植モデル / スーパーマイクロサージェリー |
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| Outline of Research at the Start |
患者腫瘍をマウスに移植することを患者由来腫瘍移植(PDX)、その中でも同所性移植する ことを患者由来腫瘍同所移植(PDOX)と呼ぶ。PDX(皮下移植)では転移を生じないが、PDOXは転移を生じる。それゆえPDOXモデルは患者の臨床病態を反映し個別化医療に最適な動物モデルである。しかし胆道癌のPDOXマウスモデルは技術的困難性のため未開発であるため、スーパーマイクロサージェリーレベルのマウス肝移植の技術を用いて本研究で胆道癌PDOXモデルの確立を行う。皮下移植モデルも作成し転移様式、転移率、増殖率を比較、PDOXモデルの妥当性を検討する。
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| Outline of Final Research Achievements |
The aim of this study was to establish a mouse model of biliary tract cancer as PDOX. Using microsurgical techniques, patient-derived biliary cancers were transplanted subcutaneously into nude mice. After the tumor grew, the tumor fragments were transplanted into the intrahepatic bile duct (liver) and extrahepatic biliary system (bile ducts and gallbladder) as PDOX by surgical suture fixation. Good engraftment and enlargement were observed within 2 months. In conclusion, the direct transplantation model to the liver (intrahepatic bile duct) was considered to be a simple model with high versatility and reproducibility in terms of technical ease and good engraftment.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究での胆道癌PDOXモデルは、胆道癌全般における理想的動物モデルとして用いることで、臨床で有効性が高い新規治療薬・治療法の開発研究が可能となる。また個々の患者においてはその未来を予測する動物モデルとして用いることが可能であり、治療法を事前に試して効果判定を行った後に実際にその患者に行うという真の個別化医療が可能となる。
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Report
(5 results)
Research Products
(89 results)
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[Journal Article] Antagonist of sphingosine 1-phosphate receptor 3 reduces cold injury of rat donor hearts for transplantation.2023
Author(s)
Eisho Kanemitsu, Xiangdong Zhao, Keiko Iwaisako, Asuka Inoue, Akihide Takeuchi, Shintaro Yagi, Hidetoshi Masumoto, Hiroaki Ohara, Motoyasu Hosokawa, Tomonari Awaya, Junken Aoki, Etsuro Hatano, Shinji Uemoto, Masatoshi Hagiwara
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Journal Title
Translational research
Volume: 255
Pages: 26-36
DOI
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Peer Reviewed / Open Access
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[Journal Article] Dynamic switch of immunity and antitumor effects of metformin in rat spontaneous esophageal carcinogenesis2022
Author(s)
Takei R, Miyashita T, Takada S, Tajima H, Ninomiya I, Takamura H, Fushida S, Harashima A, Munesue S, Yagi S, Inaki N, Ohta T, Yamamoto Y.
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Journal Title
Cancer Immunol Immunother.
Volume: 71
Issue: 4
Pages: 777-789
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Modified Socratic Method (planned and executed by Takada) for medical education: Grade II Acute Cholecystitis of Tokyo Guidelines 2018 as an example case2021
Author(s)
Takada T, Isaji S, Yoshida M, Horiguchi A, Ando H, Miyakawa S, Kiriyama S, Gomi H, Mukai S, Higuchi R, Abe Y, Okamoto K, Suzuki K, Toyota N, Hori S, Homma Y, Kato H, Umezawa A, Hata J, Inoue D, et al.
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Journal Title
J Hepatobiliary Pancreat Sci.
Volume: 2021 Nov 10.
Issue: 5
Pages: 505-520
DOI
Related Report
Peer Reviewed
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[Journal Article] De novo hepatocellular carcinoma developing in the living donor liver grafts: A Japanese multicenter experience2020
Author(s)
Goto R, Kosai-Fujimoto Y, Yagi S, Kobayashi T, Akamatsu N, Shimamura T, Imura S, Ogiso S, Mizuno S, Takatsuki M, Fukuhara T, Kanto T, Eguchi S, Yanaga K, Ogura Y, Fukumoto T, Shimada M, Hasegawa K, Ohdan H, Uemoto S, Soejima Y, Ikegami T, Yoshizumi T, Taketomi A, Maehara Y
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Journal Title
Hepatol Res
Volume: 50
Issue: 12
Pages: 1365-1374
DOI
Related Report
Peer Reviewed
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[Journal Article] Impact of preoperative bone mineral density on the outcomes after resection of pancreatic cancer.2020
Author(s)
Sharshar M, Kaido T, Shirai H, Okumura S, Yao S, Miyachi Y, Iwamura S, Kamo N, Yagi S, Macshut M, Uemoto S
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Journal Title
Surg Today
Volume: in press
Issue: 7
Pages: 757-766
DOI
Related Report
Peer Reviewed
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[Journal Article] Anatomical Pitfall in Right Posterior Sector Graft Procurement in Living Donor Liver Transplantation.2020
Author(s)
Kusakabe J, Yagi S, Okamura Y, Iida T, Ogawa E, Ito T, Hata K, Taura K, Okajima H, Kaido T, Uemoto S.
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Journal Title
Liver Transpl.
Volume: Feb;26(2)
Issue: 2
Pages: 299-303
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 肝門部領域胆管癌手術において加齢が周術期に与える影響2022
Author(s)
中沼 伸一 ,蒲田 亮介 ,高田 智司 ,岡崎 充善 ,大畠 慶直 ,岡本 浩一 ,牧野 勇 , 木下 淳 ,中村 慶史 ,田島 秀浩 ,稲木 紀幸 ,八木 真太郎
Organizer
第122回日本外科学会定期学術集会
Related Report
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