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Search for new targets of endometriosis therapy based on gene expression profile by AI

Research Project

Project/Area Number 19K22688
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
Research InstitutionYamaguchi University

Principal Investigator

Sugino Norihiro  山口大学, 大学院医学系研究科, 教授 (10263782)

Co-Investigator(Kenkyū-buntansha) 浅井 義之  山口大学, 大学院医学系研究科, 教授 (00415639)
Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords子宮内膜症 / 卵巣チョコレート嚢胞 / 遺伝子ネットワーク / バイオインフォマティクス / ブーリアンネットワーク / マスター遺伝子 / HOXC8 / TGF-β / マスターレギュレータ遺伝子 / TGF-beta / Smad経路 / Rho-ROCK経路 / 数理モデル / 人工知能
Outline of Research at the Start

近年のシステムバイオロジーの概念から、子宮内膜症の病態をマスター遺伝子の異常による遺伝子ネットワークの異常として捉えることができる。本研究では、公表されている遺伝子相互作用ネットワークと申請者が有する正常子宮内膜と子宮内膜症の遺伝子発現データを組み込んだ遺伝子制御ネットワークの数理モデルを作成することで、正常状態から疾患(子宮内膜症)を引き起こすマスター遺伝子の組み合せを人工知能を用いたシミュレーションにより同定し、また逆に、疾患から正常状態にネットワークを遷移させる遺伝子群をシミュレーションで同定することによって治療標的となるマスター遺伝子群を同定する。

Outline of Final Research Achievements

The study succeeded to identify the upstream regulators (URs) involved in the pathogenesis of ovarian endometrioma.
An analytic method, SMITE was used with transcriptome data from ovarian endometrioma stromal cells (ovESCs) and eutopic endometrium stromal cells (euESCs) in combination with publicly available gene regulatory network data. Furthermore, Boolean network simulation confirmed that the 12 candidate genes chosen by SMITE are URs. One of the URs, HOXC8, was confirmed to be overexpressed in ovESCs. HOXC8 overexpression significantly enhanced cell proliferation, migration, adhesion, and fibrotic activities, and also activated the TGF-β signaling, which are pathological features of ovarian endometrioma. The increased adhesion and fibrosis activities by HOXC8 were significantly inhibited by E-616452, a selective inhibitor of TGF-β receptor.
In conclusions, integrated genomic approaches identified HOXC8 as an UR in ovarian endometrioma.

Academic Significance and Societal Importance of the Research Achievements

子宮内膜症(卵巣チョコレート嚢胞)の発症において鍵となるマスター遺伝子を同定することができ、将来的にそれらを標的とした分子標的治療の開発に役立つ可能性がある。また、バイオインフォマティクスと数理モデルを用いた本研究の研究手法は、疾患における複雑な発現制御ネットワークを明らかにする上で、今後、更に重要となる研究手法である。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (21 results)

All 2021 2020 2019

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 9 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] The essential glucose transporter GLUT1 is epigenetically upregulated by C/EBPβ and WT1 during decidualization of the endometrium2021

    • Author(s)
      Tamura Isao、Fujimura Taishi、Doi-Tanaka Yumiko、Takagi Haruka、Shirafuta Yuichiro、Kajimura Takuya、Mihara Yumiko、Maekawa Ryo、Taketani Toshiaki、Sato Shun、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Journal of Biological Chemistry

      Volume: 297 Issue: 4 Pages: 101150-101150

    • DOI

      10.1016/j.jbc.2021.101150

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-term melatonin treatment attenuates body weight gain with aging in female mice2021

    • Author(s)
      Tamura Isao、Tamura Hiroshi、Kawamoto-Jozaki Mai、Doi-Tanaka Yumiko、Takagi Haruka、Shirafuta Yuichiro、Mihara Yumiko、Maekawa Ryo、Taketani Toshiaki、Sato Shun、Sugino Norihiro
    • Journal Title

      Journal of Endocrinology

      Volume: 251 Issue: 1 Pages: 15-25

    • DOI

      10.1530/joe-20-0462

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrated Analysis of Transcriptome and Histone Modifications in Granulosa Cells During Ovulation in Female Mice2021

    • Author(s)
      Shirafuta Yuichiro、Tamura Isao、Ohkawa Yasuyuki、Maekawa Ryo、Doi-Tanaka Yumiko、Takagi Haruka、Mihara Yumiko、Shinagawa Masahiro、Taketani Toshiaki、Sato Shun、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Endocrinology

      Volume: 162 Issue: 9 Pages: 1-17

    • DOI

      10.1210/endocr/bqab128

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between follicular size and developmental capacity of oocytes under controlled ovarian hyperstimulation in assisted reproductive technologies2021

    • Author(s)
      Tamura Isao、Kawamoto‐Jozaki Mai、Fujimura Taishi、Doi‐Tanaka Yumiko、Takagi Haruka、Shirafuta Yuichiro、Mihara Yumiko、Taketani Toshiaki、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 20 Issue: 3 Pages: 299-304

    • DOI

      10.1002/rmb2.12382

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcription factor C/EBPβ induces genome-wide H3K27ac and upregulates gene expression during decidualization of human endometrial stromal cells2021

    • Author(s)
      Tamura Isao、Maekawa Ryo、Jozaki Kosuke、Ohkawa Yasuyuki、Takagi Haruka、Doi-Tanaka Yumiko、Shirafuta Yuichiro、Mihara Yumiko、Taketani Toshiaki、Sato Shun、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 520 Pages: 111085-111085

    • DOI

      10.1016/j.mce.2020.111085

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An Integrated Genomic Approach Identifies HOXC8 as an Upstream Regulator in Ovarian Endometrioma2020

    • Author(s)
      Yumiko Mihara, Ryo Maekawa, Shun Sato, Natsuko Shimizu, Yumiko Doi-Tanaka, Haruka Takagi, Yuichiro Shirafuta, Masahiro Shinagawa, Isao Tamura, Toshiaki Taketani, Hiroshi Tamura, Takeshi Abe, Yoshiyuki Asai, Norihiro Sugino
    • Journal Title

      The Journal of Clinical Endocrinology & Metabolism

      Volume: 105 Issue: 12 Pages: 1-12

    • DOI

      10.1210/clinem/dgaa618

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Wilms tumor 1 regulates lipid accumulation in human endometrial stromal cells during decidualization2020

    • Author(s)
      Tamura Isao、Takagi Haruka、Doi-Tanaka Yumiko、Shirafuta Yuichiro、Mihara Yumiko、Shinagawa Masahiro、Maekawa Ryo、Taketani Toshiaki、Sato Shun、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Journal of Biological Chemistry

      Volume: in press Issue: 14 Pages: 4673-4683

    • DOI

      10.1074/jbc.ra120.012841

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Importance of Melatonin in Assisted Reproductive Technology and Ovarian Aging2020

    • Author(s)
      Tamura Hiroshi、Jozaki Mai、Tanabe Manabu、Shirafuta Yuichiro、Mihara Yumiko、Shinagawa Masahiro、Tamura Isao、Maekawa Ryo、Sato Shun、Taketani Toshiaki、Takasaki Akihisa、Reiter Russel J.、Sugino Norihiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 3 Pages: 1135-1135

    • DOI

      10.3390/ijms21031135

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] SATB2 and NGR1: potential upstream regulatory factors in uterine leiomyomas.2020

    • Author(s)
      Shun Sato , Ryo Maekawa , Isao Tamura , Yuichiro Shirafuta , Masahiro Shinagawa , Hiromi Asada , Toshiaki Taketani , Hiroshi Tamura , Norihiro Sugino
    • Journal Title

      Journal of assisted reproduction and genetics

      Volume: 36 Issue: 11 Pages: 2385-2397

    • DOI

      10.1007/s10815-019-01582-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] The clinical outcome of Dienogest treatment followed by in vitro fertilization and embryo transfer in infertile women with endometriosis2019

    • Author(s)
      Tamura H, Yoshida H, Kikuchi H, Josaki M, Mihara Y, Shirafuta Y, Shinagawa M, Tamura I, Taketani T, Takasaki A, Sugino N
    • Journal Title

      Journal of Ovarian Research

      Volume: 12 Issue: 1

    • DOI

      10.1186/s13048-019-0597-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome-wide DNA methylation analysis revealed stable DNA methylation status during decidualization in human endometrial stromal cells2019

    • Author(s)
      Maekawa Ryo、Tamura Isao、Shinagawa Masahiro、Mihara Yumiko、Sato Shun、Okada Maki、Taketani Toshiaki、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      BMC Genomics

      Volume: 20 Issue: 1 Pages: 324-324

    • DOI

      10.1186/s12864-019-5695-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glucose regulates the histone acetylation of gene promoters in decidualizing stromal cells2019

    • Author(s)
      Jozaki Kosuke、Tamura Isao、Takagi Haruka、Shirafuta Yuichiro、Mihara Yumiko、Shinagawa Masahiro、Maekawa Ryo、Taketani Toshiaki、Asada Hiromi、Sato Shun、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Reproduction

      Volume: 157 Issue: 5 Pages: 457-464

    • DOI

      10.1530/rep-18-0393

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aberrant DNA methylation suppresses expression of estrogen receptor 1 (ESR1) in ovarian endometrioma2019

    • Author(s)
      Maekawa Ryo、Mihara Yumiko、Sato Shun、Okada Maki、Tamura Isao、Shinagawa Masahiro、Shirafuta Yuichiro、Takagi Haruka、Taketani Toshiaki、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Journal of Ovarian Research

      Volume: 12 Issue: 1

    • DOI

      10.1186/s13048-019-0489-1

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] C/EBPβ regulates Vegf gene expression in granulosa cells undergoing luteinization during ovulation in female rats2019

    • Author(s)
      Shinagawa Masahiro、Tamura Isao、Maekawa Ryo、Sato Shun、Shirafuta Yuichiro、Mihara Yumiko、Okada - Matsumoto Maki、Taketani Toshiaki、Asada Hiromi、Tamura Hiroshi、Sugino Norihiro
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-018-36566-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Anti-aging effects on melatonin on follicle development.2021

    • Author(s)
      Sugino N
    • Organizer
      The 10th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 子宮内膜間質細胞の脱落膜化に伴う遺伝子発現調節機構2021

    • Author(s)
      杉野法広
    • Organizer
      第39回日本受精着床学会学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 子宮内膜脱落膜化とエピジェネティクス(特別講演)2020

    • Author(s)
      杉野法広
    • Organizer
      第4回 ART JAPAN 生殖医療研究会
    • Related Report
      2020 Research-status Report
  • [Presentation] 子宮内膜の発育とその異常(特別講演)2020

    • Author(s)
      杉野法広
    • Organizer
      令和2年度岡山産科婦人科学会
    • Related Report
      2020 Research-status Report
  • [Presentation] DNA methylation and uterine fibroids.2019

    • Author(s)
      Sugino N
    • Organizer
      The 8th Asian Conference on Endometriosis
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 標準産科婦人科学 第5版 綾部琢哉、板倉敦夫 編集「ホルモン療法」2021

    • Author(s)
      杉野法広
    • Total Pages
      697
    • Publisher
      医学書院
    • Related Report
      2021 Annual Research Report
  • [Book] 生殖と周産期のリエゾン 編集:池田智明、苛原稔、吉村泰典2020

    • Author(s)
      前川 亮 杉野法広
    • Total Pages
      8
    • Publisher
      診断と治療社
    • Related Report
      2020 Research-status Report

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Published: 2019-07-04   Modified: 2023-01-30  

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