• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Utilization of gas mixture with low hydrogen content for microbial carbon dioxide fixation

Research Project

Project/Area Number 19K22923
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 64:Environmental conservation measure and related fields
Research InstitutionTokyo Institute of Technology

Principal Investigator

Tsuge Takeharu  東京工業大学, 物質理工学院, 准教授 (70332260)

Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywordsポリヒドロキシアルカン酸 / 生分解性プラスチック / 化学合成独立栄養細菌 / ポリエステル合成
Outline of Research at the Start

本研究では、二酸化炭素を効率的に資源化することを目指し、高付加価値物質に直接変換することができる生物固定化に着目した。なかでも、高い炭酸固定能、高い増殖速度、そして、その細胞内に80%以上の含有率で生分解性プラスチック(ポリヒドロキシアルカン酸、PHA)を合成する化学合成独立栄養細菌に着目し、低濃度水素を有効に活用することで安全に炭酸固定が可能な手法の開発を行う。

Outline of Final Research Achievements

This study aimed to use gas mixture with low hydrogen content for autotrophic culture of hydrogen-oxidizing bacteria. The lower explosive limit of hydrogen is 4%, at which hydrogen can be safely handled during cultivation. However, the ability of carbon dioxide fixation by the hydrogen-oxidizing bacteria at low hydrogen concentration has not been investigated. This study validated the use of low-concentration hydrogen gas mixture in terms of the bacterial cell growth and biopolyester production.

Academic Significance and Societal Importance of the Research Achievements

低濃度水素を用いた独立栄養培養法は、水素の爆発を回避し、安全な培養技術としてのプラットフォームになり得るものである。水素の爆発下限界での効率的な培養法が確立できれば、水素細菌による物質生産系は非常に大きな発展性を有している。また、太陽光などの再生可能なエネルギーから水の電気分解によって生産されたグリーン水素を利用すれば、究極の持続可能な物質生産系として環境保全に大きく貢献する。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (6 results)

All 2021 2020

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] The gene dosage effect of carbonic anhydrase on the biosynthesis of poly(3-hydroxybutyrate) under autotrophic and mixotrophic culture conditions2021

    • Author(s)
      Romeo Thorbecke, Masahiro Yamamoto, Yuki Miyahara, Mino Oota, Shoji Mizuno, Takeharu Tsuge.
    • Journal Title

      Polymer Journal

      Volume: 53 Issue: 1 Pages: 209-213

    • DOI

      10.1038/s41428-020-00409-3

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Autotrophic biosynthesis of polyhydroxyalkanoate by Ralstonia eutropha from non-combustible gas mixture with low hydrogen content2020

    • Author(s)
      Yuki Miyahara, Masahiro Yamamoto, Romeo Thorbecke, Shoji Mizuno, Takeharu Tsuge
    • Journal Title

      Biotechnology Letters

      Volume: - Issue: 9 Pages: 1655-1662

    • DOI

      10.1007/s10529-020-02876-3

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] Autotrophic biosynthesis of biodegradable polyester by Ralstonia eutropha from a non-explosive gas mixture2021

    • Author(s)
      Yuki Miyahara, Masahiro Yamamoto, Thorbecke Romeo, Shoji Mizuno, Takeharu Tsuge
    • Organizer
      Pacifichem 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非爆発性混合ガスを用いた二酸化炭素からの生分解性ポリエステル生合成2021

    • Author(s)
      宮原 佑宜、山本 真大、Thorbecke Romeo、水野 匠詞、柘植 丈治
    • Organizer
      第10回JACI/GSCシンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Autotrophic biosynthesis of PHA with low hydrogen supply2020

    • Author(s)
      Romeo Thorbecke、山本真大、宮原佑宜、水野匠詞、柘植丈治
    • Organizer
      エコマテリアルシンポジウム
    • Related Report
      2019 Research-status Report
  • [Book] バイオリアクターのスケールアップと物質生産事例集2021

    • Author(s)
      宮原佑宜, 柘植丈治ほか80名
    • Total Pages
      515
    • Publisher
      技術情報協会
    • ISBN
      9784861048302
    • Related Report
      2021 Annual Research Report

URL: 

Published: 2019-07-04   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi