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Continuous generation of single-cell-laden microgels using micro pillar arrays

Research Project

Project/Area Number 19K23585
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0402:Nano/micro science, applied condensed matter physics, applied physics and engineering, and related fields
Research InstitutionKyushu University (2020)
Tokyo Institute of Technology (2019)

Principal Investigator

Tottori Naotomo  九州大学, 工学研究院, 助教 (00840646)

Project Period (FY) 2019-08-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsマイクロナノデバイス / ゲル微粒子 / 粒子分離 / 微粒子分離 / 流体工学 / 粒子処理 / 単一細胞
Outline of Research at the Start

マイクロ流路を用いた単一細胞包埋ゲル微粒子の作製手法は,従来困難であった単一細胞レベルでの培養,解析,および組織構築を可能とするため,医学・生化学などの基礎研究,再生医療用途等,幅広い分野での応用が期待されている.本研究では,支柱配列を用いた粒子軌道制御法を応用し,単一細胞包埋ゲル微粒子を迅速,簡便かつ高効率に作製可能な新規プロセス技術を確立する.また,培養試験により作製プロセスが細胞に与える影響を評価する.

Outline of Final Research Achievements

In this study, we demonstrated separation of single-cell-encapsulated droplets, and continuous generation of cell-laden microgels using micropillar arrays. Water-in-oil droplets containing cells were produced in a microchannel and subsequently introduced into the micorpillar arrays to separate cell-encapsulated droplets from empty droplets. In addition, spherical cell-laden microgel particles dispersed in aqueous phase without satellite droplets were obtained by transporting precusor main droplets across the streams of the reaction solution and washing aqueous solution using micropillar arrays.

Academic Significance and Societal Importance of the Research Achievements

従来のマイクロ流路を用いた単一細胞包埋ゲル微粒子の作製手法では,単一細胞の包埋効率は低く,また,ゲル微粒子の作製工程において,煩雑なバッチ式作業による,粒子洗浄・溶液置換が必要であるといった課題がある.対して本研究は,単一細胞包埋ゲル微粒子を迅速,簡便かつ高効率に作製可能な手法の確立を目指す,前例のない研究であり,提案するプロセス技術は,医学・生化学などの基礎研究,再生医療への応用など,新しい分野の開拓・発展を支える基盤技術になり得ると考えられる.

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2020 2019

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 2 results) Book (1 results)

  • [Journal Article] Nanoparticle Separation through Deterministic Lateral Displacement Arrays in Poly(dimethylsiloxane)2020

    • Author(s)
      Naotomo Tottori , Yasuhiko Muramoto, Hiraku Sakai, and Takasi Nisisako
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 53 Issue: 8 Pages: 414-421

    • DOI

      10.1252/jcej.19we160

    • NAID

      130007889022

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Particle/cell separation using sheath-free deterministic lateral displacement arrays with inertially focused single straight input2020

    • Author(s)
      Naotomo Tottori, and Takasi Nisisako
    • Journal Title

      Lab on a Chip

      Volume: 20 Issue: 11 Pages: 1999-2008

    • DOI

      10.1039/d0lc00354a

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Editorial for the Special Issue on Particles Separation in Microfluidic Devices2020

    • Author(s)
      Naotomo Tottori, and Takasi Nisisako
    • Journal Title

      Micromachines

      Volume: 11 Issue: 6 Pages: 602-602

    • DOI

      10.3390/mi11060602

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Presentation] Lactic acid bacteria of different shpaes flowing through deterministic lateral displacement microarrays2020

    • Author(s)
      Guangchong Ji, Naotomo Tottori, and Takasi Nisisako
    • Organizer
      The 18th International Conference on Precision Engineering (ICPE2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Continuous generation of cell-laden microgels through deterministic lateral displacement arrays2020

    • Author(s)
      Naotomo Tottori, and Takasi Nisisako
    • Organizer
      The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Deterministic lateral displacement separation of lactic acid bacteria from yogurt sample after vortexing2020

    • Author(s)
      Guangchong Ji, Naotomo Tottori, and Takasi Nisisako
    • Organizer
      2020年度精密工学会春季大会学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] マイクロピラーアレイを用いたアルギン酸カルシウムゲル粒子の連続生成2019

    • Author(s)
      鳥取直友,西迫貴志
    • Organizer
      化学とマイクロ・ナノシステム学会第40回研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Isolation of lactic acid bacteria from yogurt using a deterministic lateral displacement array2019

    • Author(s)
      Guangchong Ji, Naotomo Tottori, and Takasi Nisisako
    • Organizer
      化学とマイクロ・ナノシステム学会第40回研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Separation of lactic acid bacteria from yogurt through deterministic lateral displacement micropillar arrays2019

    • Author(s)
      Guangchong Ji, Naotomo Tottori, and Takasi Nisisako
    • Organizer
      2019年度精密工学会秋季大会学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] DLDマイクロピラーアレイを用いたハイドロゲル微粒子の生成と溶液置換2019

    • Author(s)
      鳥取直友,西迫貴志
    • Organizer
      2019年度精密工学会秋季大会学術講演会
    • Related Report
      2019 Research-status Report
  • [Book] フロー合成、連続生産のプロセス設計、条件設定と応用事例2020

    • Author(s)
      鳥取直友,西迫貴志(分担執筆)
    • Total Pages
      599
    • Publisher
      技術情報協会
    • ISBN
      9784861048203
    • Related Report
      2020 Annual Research Report

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Published: 2019-09-03   Modified: 2022-01-27  

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