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Discovery of novel secondary metabolites from extremophiles by genome mining

Research Project

Project/Area Number 19K23671
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0601:Agricultural chemistry and related fields
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Ueoka Reiko  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (30592365)

Project Period (FY) 2019-08-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords天然物化学 / ゲノムマイニング
Outline of Research at the Start

創薬において天然由来の化合物は医薬リ-ド化合物として非常に重要な役割を持つ。本研究では、これまで比較的探索源として活用されて来なかった好極限性菌、特に中高度好塩菌を対象に、ゲノムマイニング法を用いてユニ-クな天然化合物の探索研究を行う。

Outline of Final Research Achievements

In this study, we focused on the halophiles to discover novel secondary metabolites for drug leads. Detailed analysis of the genomes from NCBI database showed the potent halophiles with the orphan biosynthetic gene clusters in their genomes. Selected halophiles were cultured in the different conditions, and the extracts were analyzed to find new natural products. Also, the marine bacteria, especially Pseudoalteromonadaceae, residing in Okinawan waters were studied because of the high number of biosynthetic gene clusters. From those bacteria, the extract from Pseudoalteromonas piscicida showed the presence of a novel metabolite. The mass spectrometry-guided fractionations yielded a new lipopeptide pseudoalteropeptide A, and its bioactivity was evaluated.

Academic Significance and Societal Importance of the Research Achievements

天然資源からの新規化合物の探索が困難になりつつある現在においても、未利用な天然資源を探索源とし、ゲノム情報を活用することで、より効率的な新規化合物の探索ができる可能性を示すことができた。ゲノム情報が広く公開されている微生物においても、生合成遺伝子を詳細に解析することで、新規化合物を生産しうる微生物が種々存在することが分かり、これらから、今後もさらなる新規化合物が発見されることが期待される。

Report

(3 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • Research Products

    (1 results)

All 2021

All Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] Pseudoalteropeptide A, a novel lipopeptide from the marine bacterium Pseudoalteromonas piscicida SWA4_PA4 isolated from marine seaweed2021

    • Author(s)
      Reiko Ueoka, Naoya Shinzato, Noritaka Kagaya, Hikaru Suenaga, Kazuo Shin-ya
    • Journal Title

      The Journal of Antibiotics

      Volume: 74 Issue: 2 Pages: 105-110

    • DOI

      10.1038/s41429-020-00377-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2019-09-03   Modified: 2022-01-27  

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