| Project/Area Number |
19K23750
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0702:Biology at cellular to organismal levels, and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
QIAN PINGPING 大阪大学, 理学研究科, 助教 (20840466)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | DOF transcription factor / CLE peptide / LRR-RLK receptor / BAM / CIK / vasculature / phloem / cell patterning / transcription factor / peptide / vasculature development / root / Arabidopsis |
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| Outline of Research at the Start |
As an integral transport system, plant vasculature plays a crucial role in their development and growth. We focus on a number of DOF transcription factors that regulate periclinal cell division of phloem development. DOFs directly target to some CLE genes and activate their expression, and these peptides may be perceived by BAM receptors to repress DOFs, forming a negative feedback loop. CLEs repress DOFs in a post-translational event, perhaps involving protein phosphorylation and degradation.I will prove this negative feedback loop of CLEs-BAMs-Dofs signaling cascade in vascular development.
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| Outline of Final Research Achievements |
The regulatory mechanisms of phloem development are being uncovered. We find that a series of phloem-enriched DOF transcription factors (P-Dofs) not only regulate the number of procambium cell files, but also are necessary and sufficient for phloem differentiation. Overexpression of phloem-Dofs induced cells that expressed either sieve element or companion cell marker genes, which are mutually exclusive. Conversely, disruption of phloem-Dofs caused loss of phloem. Phloem-Dofs induce CLE25/26/45 peptides, which in turn inhibit expression of phloem-Dofs and phloem formation, forming a negative feedback loop. Disruption of multiple genes for either phloem-expressed CLEs, BAM-class receptors, or their coreceptors, CIKs, caused excess formation of phloem cell files. We further show that P-Dofs are under positive self and mutual regulation. These positive and negative feedback loops create the proper phloem pattern. There are more redundant DOFs, CLEs, and BAMs/CIKs involved in this pathway.
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| Academic Significance and Societal Importance of the Research Achievements |
Our results close a major gap in our understanding of phloem development in root primary growth.It is a good start for further studying the molecular mechanism of whole process of root vascular development and patterning.It also has strong theoretical guiding significance for future crop breeding.
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