Colloidal Platinum Nanoparticles and Phospphorylated Pullulan improve the pulp capping capability of Mineral trioxide Aggregate

• Project/Area Number: 19K24110
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Hokkaido University
• Principal Investigator: CHOWDHURY Abu Faem Mohammad Almas 北海道大学, 歯学研究院, 博士研究員 (00844440)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Pulp capping / MTA / Inflammation / Mineralization / CPN / Pulpal response / Pullulan (PL) / Biocompatibility

Outline of Research at the Start

To evaluate:
rats’ pulpal response to capping with a new MTA containing PL and CPN,
adhesive ability of both PL and CPN by microtensile bond strength test employing extracted human molars,
sealing capability of PL and CPN by SEM and TEM,
biocompatibility of CPN by using human immortalized pulp cells.

Outline of Final Research Achievements

Study 1 demonstrated that Colloidal Platinum Nanoparticles (CPN) showed favorable healing responses in Wister rat's dental pulp. CPN's healing responses were comparable to the current "gold standard" pulp capping material - Mineral trioxide aggregate (MTA). In Study 2, the confirmation of the presence of CD34 endothelial cells revealed vasculogenesis, while the presence of DMP1 revealed mineralized tissue induction - the hallmarks of healing. Our findings indicated the suitability of the MTA and Phosphorylated Pullulan (PPL) combination in pulpal healing after exposure.

Academic Significance and Societal Importance of the Research Achievements

今回の研究では、ウィスター系ラットを用いて確立された実験モデルを適用しました。これを用いて、新規材料に対する歯髄の反応について詳細に検討しました。今回の研究結果によると、PLとPtの双方が良好な歯髄反応をもたらしました。PLとPtをMTAの合材に用いると、覆髄材料の接着能力が向上し、殺菌特性が追加され、更なる細菌の侵入が防止される可能性があります。覆髄材にこの組み合わせを採用することで、より多くの虫歯や外傷で傷ついた歯を救うことができると考えられます。

Research Products

• [Presentation] Effects of Colloidal Platinum Nanoparticles on Dental Pulp (2020)
  • Author(s): R. Islam, Y. Toida, C. Fei, S. Hoshika, T. Ikeda, C. Watanabe, T. Tanaka, M. Matsumoto, M. Yamauti, A. A. Chowdhury, H. Sano
  • Organizer: The 98th General Session of the IADR
  • Int'l Joint Research
• [Presentation] Effects of Colloidal Platinum Nanoparticles (2019)
  • Author(s): Abu Faem Mohammad Almas Chowdhury
  • Organizer: 98th General session of the IADR
  • Int'l Joint Research
• [Presentation] From the land of royal bengal tigers to the land of rising sun: a reseracher's odyssey (2019)
  • Author(s): Abu Faem Mohammad Almas Chowdhury
  • Organizer: Lecture, University of Granada, Spain
  • Invited