The in vitro/in vivo study of CCN2 on dentin regeneration
| Project/Area Number |
19K24127
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
QIU YOUJING 北海道医療大学, 歯学部, 研究員 (90845421)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | dental materials / dentin regeneration / odontoblasts / direct pulp capping / adhesives / bioactive materials / CCN2 / odontoblast |
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| Outline of Research at the Start |
In this research, we will use cell biology and animal experiment technology to investigate the in vitro and in vivo effect of CCN2 on odontoblast cells and pulp cells regarding proliferation, differentiation, and mineralization. We will also explore the effect of CCN2 on pulpitis in vitro model.
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| Outline of Final Research Achievements |
I investigated the in vitro/in vivo effect of novel bioactive materials on MDPC-23 cells, human dental pulp stem cells (hDPSCs), and wistar rats as planned. The investigation of a novel adhesive monomer CMET on MDPC-23 cells has revealed that CMET stimulated MDPC-23 cells proliferation, differentiation and mineralization. The results have been published on on International Endodontic Journal (Int Endod J. 2020 Oct; 53(10):1413-1429). The investigation on hDPSCs has showed that CMET has low cytotoxicity and exhibits high activity on stimulation of odontogenic differentiation and matrix mineralization. The in vivo study on wistar rats showed that direct capping with CMET can effectively promote dentin bridge
formation.The results of the effect of CMET on dentin regeneration is under submission to SCI journal and patent application.
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| Academic Significance and Societal Importance of the Research Achievements |
The successful completion of these studies will provide valuable results for the investigation of the development of novel dental materials. Besides, the result of these studies may change the current situation of direct pulp capping, and also provide new ideas for clinical treatments.
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Report
(3 results)
Research Products
(11 results)
