| Outline of Final Research Achievements |
This study aimed to investigate the roles of (liquid-liquid) phase separation (LLPS) in regulation of regulatory elements in the genome and, particularly, super-enhancers toward better understanding of alteration in gene regulation and biomolecular condensates in cancers. By focusing on the RNA exosome, which degrades many unstable RNAs in the nucleus, we have identified the regulatory roles of the RNA exosome in regulation of super-enhancers and transcriptional condensates. We have also studied the roles of intrinsically disordered regions (IDRs), major drivers of LLPS, and extrachromosomal circular DNAs (eccDNAs) in cancer cells. Furthermore, by expanding the expertise in computational modeling in previous studies, additional collaboration project has successfully developed new CRISPR-based genome editing strategy, called “safeguard sgRNA” strategy, which improves the safety and applicability of genome editing.
|
