Biological Control of Dengue Disease Using Symbiotic Wolbachia Bacteria: Environmentally Safe and Inexpensive Approach

• Project/Area Number: 19KK0107
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 22:Civil engineering and related fields
• Research Institution: Ehime University
• Principal Investigator: Watanabe Kozo 愛媛大学, 沿岸環境科学研究センター, 教授 (80634435)
• Co-Investigator(Kenkyū-buntansha): 糠澤 桂 宮崎大学, 工学部, 准教授 (20725642) ; 八重樫 咲子 山梨大学, 大学院総合研究部, 准教授 (30756648) ; ガンボア・メンデス マリベット・アリアナ 愛媛大学, 理工学研究科(工学系), 助教 (30849477)
• Project Period (FY): 2019-10-07 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2021: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2020: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: デング熱 / ネッタイシマカ / フィリピン / ボルバキア / 蚊媒介感染症 / 金ナノ粒子 / 集団遺伝解析

Outline of Research at the Start

近年，蚊からヒトへのデングウイルス感染を抑制する昆虫共生細菌ボルバキアが注目されている。本研究は，フィリピン・マニラを対象として，当地のネッタイシマカに自然感染したボルバキアを活用した安心・安価なデング熱制御技術を開発する。ボルバキアに自然感染した蚊幼虫を見つけ，施設で繁殖して都市に放虫するシステムである。この構築のため，自然感染した幼虫を金ナノ粒子で迅速・安価に選別する技術を米国・ミシガン州立大と，蚊の飛翔範囲を推定して最適放虫地点を決定する蚊ゲノム解析技術をドイツ・ベルリンゲノムセンターと開発する。蚊採取と繁殖実験はフィリピンで行い，4か国の国際研究拠点を形成する。

Outline of Final Research Achievements

The purpose of this study is to develop a safe and inexpensive technique to control dengue fever epidemics by utilizing the mosquito-symbiotic bacterium Wolbachia strains infected in the dengue vector Aedes aegypti. The effectiveness of the simple Wolbachia detection method using gold nanoparticles proposed by this study was confirmed. Population genetic analysis of the Aedes aegypti in Manila also revealed that the flight of mosquitoes crossing large roads was limited. In addition, real-time PCR quantification of Wolbachia concentrations in 429 adults Aedes aegypti collected in Metro Manila revealed significant differences in Wolbachia concentrations in mosquito bodies among three Wolbachia strain groups among the 267 individuals that tested positive for Wolbachia.

Academic Significance and Societal Importance of the Research Achievements

ネッタイシマカに自然感染した蚊共生細菌ボルバキアを活用してデング熱流行を制御する手法の活用に繋がる様々な発見をした。特に、ネッタイシマカ体内で高濃度になりやすいボルバキア株があったことは、蚊体内において効率的にデングウイルス濃度を制御するために、これらのボルバキア株の活用が有効だることを示唆する有用な発見となった。

Research Products

• [Int'l Joint Research] デラサール大学/フィリピン大学マニラ校(フィリピン)
• [Int'l Joint Research] ライプニッツ淡水生態学・内水 漁業研究所(ドイツ)
• [Int'l Joint Research] ミシガン州立大学(米国)
• [Int'l Joint Research] デラサール大学/フィリピン大学マニラ校/フィリピン大学ロスバニョス校(フィリピン)
• [Int'l Joint Research] ライプニッツ淡水生態学・内水漁業研究所(ドイツ)
• [Int'l Joint Research] ミシガン州立大学(米国)
• [Journal Article] Detection and phylogeny of Wolbachia in field-collected Aedes albopictus and Aedes aegypti from Manila City, Philippines (2022)
  • Author(s): Regilme, M. A. F., T. Inukai and K. Watanabe
  • Journal Title: European Journal of Molecular & Clinical Medicine Volume: accepted
  • Peer Reviewed / Open Access
• [Journal Article] Temporal Correlation between Urban Microclimate, Vector Mosquito Abundance, and Dengue Cases (2022)
  • Author(s): Faridah, L, N. Fauziah, D. Agustian, I. G. N. M. Jaya, R. E. Putra, S. Ekawardhani, N. Hidayath, I. D. Djati, T. M. Carvajal, R. Agoes and K. Watanabe
  • Journal Title: Journal of Medical Entomology Volume: in press Issue: 3 Pages: 1008-1018
  • DOI: 10.1093/jme/tjac005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative population genetic structure of two ixodid tick species (Acari:Ixodidae) (Ixodes ovatus and Haemaphysalis flava) in Niigata Prefecture, Japan (2021)
  • Author(s): Regilme, M. A. F., M. Sato, T. Tamura, R. Arai, M. O. Sato, S. Ikeda, M. Doi, K. Tanaka, M. Gamboa, M. T. Monaghan, and K. Watanabe
  • Journal Title: Infection, Genetics and Evolution Volume: 94 Pages: 104999-104999
  • DOI: 10.1016/j.meegid.2021.104999
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dengue Disease Dynamics are Modulated by the Combined Influence of Precipitation and Landscapes: A Machine Learning-based Approach (2021)
  • Author(s): Francisco, M. E., T. M. Carvajal, M. Ryo, K. Nukazawa, D. M. Amalin, and K. Watanabe
  • Journal Title: Science of the Total Environment Volume: 792 (20) Pages: 148406-148406
  • DOI: 10.1016/j.scitotenv.2021.148406
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Carotenoid Coloration and Coloration-Linked Gene Expression in Red Nile Tilapia (Oreochromis niloticus) Tissues (2021)
  • Author(s): Cruz, K. G. J., E. P. Landingin, M. B. Gajeton, S. I. D. Fernando, and K. Watanabe
  • Journal Title: BMC Veterinary Research Volume: 17 Issue: 1 Pages: 314-314
  • DOI: 10.1186/s12917-021-03006-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Next Generation of AMR Network (2021)
  • Author(s): Payumo, J. G., E. Alocilja, C. Boodoo, K. Luchini-Colbry, P. Ruegg, E. Mclamore, D. V. Gamboa, R. K. Briceno, A. Castaneda-Sabogal, K. Watanabe, M. J. Gordoncillo, D. Amalin, L. Fernando, and N. Bhusal
  • Journal Title: Encyclopedia Volume: 1 (3) Issue: 3 Pages: 871-892
  • DOI: 10.3390/encyclopedia1030067
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Intracellular Interactions between Arboviruses and Wolbachia in Aedes aegypti (2021)
  • Author(s): Reyes, J. I. L., Y. Suzuki, T. M. Carvajal, M. N. M. Munoz, and K. Watanabe
  • Journal Title: Frontiers in Cellular and Infection Microbiology Volume: 11 Pages: 690087-690087
  • DOI: 10.3389/fcimb.2021.690087
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acute Oral Toxicity Assessment of Ethanolic Extracts of Antidesma bunius (L.) Spreng Fruits in Mice in vivo (2021)
  • Author(s): Mu?oz, M. N. M., U. A. Tejada, J. I. L. Reyes, and K. Watanabe
  • Journal Title: Toxicology Reports Volume: 8 Pages: 1289-1299
  • DOI: 10.1016/j.toxrep.2021.06.010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Demographic and Spatial Pattern Analysis of Hospitalized Dengue Patients in Bandung City in the Year 2014-2016 (2021)
  • Author(s): Faridah, L., I. G. N. Mindra, R. E. Putra, N. Fauziah, D. Agustian, Y. A. Natalia, and K. Watanabe
  • Journal Title: Tropical Medicine and Health Volume: 49 Issue: 1 Pages: 44-44
  • DOI: 10.1186/s41182-021-00329-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biofilm Inhibition in Candida albicans by Biologically-synthesized Gold Nanoparticles (2021)
  • Author(s): Cruz, K. G. J., E. D. Alfonso, S. I. D. Fernando, and K. Watanabe
  • Journal Title: Frontiers in Microbiology Volume: 12 Pages: 665113-665113
  • DOI: 10.3389/fmicb.2021.665113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ticks Prevalence in Niigata Prefecture, Japan (2016 ? 2018), the Species Shifting from the 1950s (2021)
  • Author(s): Sato, M., S. Ikeda, R. Arai, M. Kato, J. Aoki, A. Nishida, K. Watanabe, C. Hirokawa, K. Watanabe, M. A. F. Regilme, M. Sato, M. O. Sato, T. Tamura
  • Journal Title: Ticks and Tick-borne Diseases Volume: 12 (3) Issue: 3 Pages: 101683-101683
  • DOI: 10.1016/j.ttbdis.2021.101683
  • Peer Reviewed / Open Access
• [Journal Article] Intracellular Interactions between Arboviruses and Wolbachia in Aedes aegypti (2021)
  • Author(s): Reyes, J. I. L., Y. Suzuki, , T. M. Carvajal, M. N. M. Munoz, and K. Watanabe
  • Journal Title: prerprints Volume: n.a.
  • DOI: 10.20944/preprints202104.0151.v1
  • Open Access / Int'l Joint Research
• [Journal Article] Early Detection of Dengue Fever Outbreaks Using a Surveillance App (Mozzify): Cross-sectional Mixed Methods Usability Study (2021)
  • Author(s): Herbuela, V. R. D. M., T. Karita, T. M. Carvajal, H. T. Ho, J. D. Lorena, R. A. Regalado, G. D. Sobrepe?a, and K. Watanabe
  • Journal Title: JMIR Public Health and Surveillance Volume: 7 (3) Issue: 3 Pages: e19034-e19034
  • DOI: 10.2196/19034
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The influence of roads on the fine-scale population genetic structure of the dengue vector Aedes aegypti (Linnaeus) (2021)
  • Author(s): Regilme, M. A. F., T. M. Carvajal, A. C. Honnen, D. M. Amalin, and K. Watanabe
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 15(2) Issue: 2 Pages: e0009139-e0009139
  • DOI: 10.1371/journal.pntd.0009139
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Underdiagnosis does not account for the decrease in dengue cases in Bandung, Indonesia (2021)
  • Author(s): Faridah, L., S. Ekawardhani, H. L. Wiraswati, N. Fauziah, F. R. Rinawan, W. Mayasarid, and K. Watanabe
  • Journal Title: Systematic Reviews in Pharmacy Volume: 12(1) Pages: 1340-1342
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spotted fever group Rickettsiae (SFGR) found in a follow-up investigation of in ticks after the occurrence of Japanese spotted fever human case in Niigata Prefecture, Japan (2021)
  • Author(s): Arai, R., M. Hayashi, M. Kato, J. Aoki, A. Nishida, K. Watanabe, C. Hirokawa, S. Ikeda, T. Tamura, K Watanabe, M. A. F. Regilme, M. O. Sato, and M. Sato
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 2595-2595
  • DOI: 10.1038/s41598-021-81587-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Wing Geometry and Genetic Analyses Reveal Contrasting Spatial Structures between Male and Female Aedes aegypti Populations in Metropolitan Manila, Philippines (2021)
  • Author(s): Carvajal, T. M., D. M. Amalin, and K. Watanabe
  • Journal Title: Infection, Genetics and Evolution Volume: 87 Pages: 104676-104676
  • DOI: 10.1016/j.meegid.2020.104676
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Surveillance of Dengue Virus in Individual Aedes aegypti Mosquitoes Collected Concurrently with Suspected Human Cases in Tarlac City, Philippines (2020)
  • Author(s): Balingit,J. C., T. M. Carvajal, M. Saito-Obata, M. Gamboa, A. D. Nicolasora, A.K. Sy, H. Oshitani, and K. Watanabe
  • Journal Title: Parasites & Vectors Volume: 13 Issue: 1 Pages: 594-594
  • DOI: 10.1186/s13071-020-04470-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fine-Scale Population Genetic Structure of Dengue Mosquito Vector, Aedes aegypti in Metropolitan Manila, Philippines (2020)
  • Author(s): Carvajal, T. M., K. Ogishi, S. Yaegashi, L. F. T. Hernandez, K. M. Viacrusis, H. T. Ho, D. Amalin, and K. Watanabe
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 4 Issue: 5 Pages: e0008279-e0008279
  • DOI: 10.1371/journal.pntd.0008279
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative population genetic structure of two Ixodidae ticks (Ixodes ovatus and Haemaphysalis flava) in Niigata Prefecture, Japan (2020)
  • Author(s): Regilme, M. A. F., M. Sato, T. Tamura, R. Arai, M. O. Sato, S. Ikeda, M. Doi, K. Tanaka, M. Gamboa, M. T. Monaghan, and K. Watanabe
  • Journal Title: bioRxiv Volume: n.a.
  • DOI: 10.1101/862904
  • Open Access
• [Journal Article] Understanding Hurdles in the Implementation of Health Information System (HIS) Through an Institutional Framework: A Case of Bandung, West Java Province, Indonesia (2020)
  • Author(s): Faridah, L, F. Rinawan, N. Fauziah, W. Mayasari, A. Dwiartama, K. Watanabe
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 17(5) Issue: 5 Pages: 1795-1795
  • DOI: 10.3390/ijerph17051795
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An Integrated mHealth App for Dengue Reporting and Mapping, Health Communication, and Behavior Modification: Development and Assessment of Mozzify (2020)
  • Author(s): Herbuela, V. R. D. M., T. Karita, M. Francisco, and K. Watanabe
  • Journal Title: JMIR Formative Research Volume: 4(1)
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Depressive and Anxiety Symptoms among Pediatric In-patients with Dengue Fever: A Case-control Study (2019)
  • Author(s): Herbuela, V.R.D.M., F.S.d. Guzman, G. Sobrepe?a, A.B.F. Claudio, A.C.V. Tomas, C.M.A-d. Reyes, M.M. Teodoro, and K. Watanabe
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 17 (1) Issue: 1 Pages: 99-99
  • DOI: 10.3390/ijerph17010099
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Knowledge, Attitude and Practices on Dengue Fever among Paediatric and Adult In-patients in Metro Manila, Philippines (2019)
  • Author(s): Herbuela, V. R. D. M., F. S. d Guzman, G. D. Sobrepena, A. B. F. Claudio, A. C. V. Tomas, C. M. A-d Reyes, R. A. Regalado, M. M. Teodoro, and K. Watanabe
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 16 (23) Issue: 23 Pages: 4705-4705
  • DOI: 10.3390/ijerph16234705
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of Wolbachia Infection in Field-collected Mosquito Vector, Aedes aegypti in Metropolitan Manila, Philippines (2019)
  • Author(s): Carvajal,T. M., K. Hashimoto, R. K. Harnandika, D. M. Amalin and K. Watanabe
  • Journal Title: Parasites & Vectors Volume: 12 (1) Issue: 1 Pages: 361-361
  • DOI: 10.1186/s13071-019-3629-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Is Rapid Dengue Test Necessary at Primary Health Centre? A Study In Bandung City (2019)
  • Author(s): Faridah, L., N. Fauziah, S Ekawardhani, Riyadi, and K. Watanabe
  • Journal Title: Journal of Clinical and Diagnostic Research Volume: 13 (7)
  • DOI: 10.7860/jcdr/2019/41059.13004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Eco-epidemiology of Dengue Mosquitoes in Metropolitan Manila, Philippines (2022)
  • Author(s): Kozo Watanabe
  • Organizer: Trinity University of Asia's Research Congress (TRIICON)
  • Int'l Joint Research / Invited
• [Presentation] Spatio-temporal and Numerical Data Resolution Affect the Performance of Machine Learning in Dengue Forecast. A Case Study in Metropolitan Manila, Philippines. (2022)
  • Author(s): Francisco ME, Carvajal TM, Watanabe K
  • Organizer: International Seminar on Conducting Scientific Research ? Ecological Approaches to Mosquito-borne Disease Control JSPS Core-to-Core Program B. Asia ? Africa Science Platforms
  • Int'l Joint Research
• [Presentation] Viral DNA forms of cell fusing agent virus (CFAV) are produced in Aedes aegypti mosquito cell lines (2021)
  • Author(s): Mohammad Mosleh Uddin, Yasutsugu Sujuki, Kozo Watanabe
  • Organizer: The 44th Annual Meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research
• [Presentation] Inference of Spatial and Temporal Dataset Granularity Effect to the Performance of Machine Learning in Dengue Forecast (2021)
  • Author(s): Micanaldo Ernesto Francisco, Thaddeus M. Carvajal, and Kozo Watanabea
  • Organizer: 2nd JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research / Invited
• [Presentation] The influence of roads on the fine-scale population genetic structure of the dengue vector Aedes aegypti (Linnaeus) (2021)
  • Author(s): Maria Angenica Fulo Regilme, Thaddeus Carvajal , Ann?Christin Honnen, Divina Amalin, Kozo Watanabe
  • Organizer: 2nd JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research / Invited
• [Presentation] The Influence of Water Supply Conditions and Water Storage Containers toward Vector Prevalence and Abundance in Makassar City, Indonesia (2021)
  • Author(s): La Ane, R., I. Wahid, A. Susilawaty, H. Ishak, E. Ibrahim, M-C Chiu, V. R. D. M. Herbuela, and K. Watanabe
  • Organizer: 2nd JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research / Invited
• [Presentation] The Time Varying Effect of Weather Variables and its Interaction on Dengue Occurrence During 2009-2015, in Bandung City, Indonesia (2021)
  • Author(s): Agustian D, Jaya IGM, Permana YS, Faridah L, Agoes R, Watanabe K
  • Organizer: 5th International Conference on Climate Change 2021 (ICCC 2021)
  • Int'l Joint Research
• [Presentation] Comparative population genetic structure of Ixodes ovatus and Haempahysalis flava in Niigata Prefecture (2020)
  • Author(s): Maria Angenica Fulo Regilme , Megumi Sato , Tsutomu Tamura , Reiko Arai , Marcello Otake Sato , Sumire Ikeda , Masaya Doi, Kohki Tanaka , Maribet Gamboa , Michael T. Monaghan, Kozo Watanabe
  • Organizer: The 43rd Annual Meeting of the Molecular Biology Society of Japan (MBSJ online)
  • Int'l Joint Research
• [Presentation] Molecular Genetic Approaches to the Assessment of Dam Impact on Stream Macroinvertebrates and Dengue Virus Surveillance of Vector Mosquitoes (2020)
  • Author(s): Kozo Watanabe
  • Organizer: 47th Philippine Society of Biochemistry and Molecular Biology (PSBMB) Annual Convention
  • Int'l Joint Research / Invited
• [Presentation] Wing Geometry and Genetic Analyses Reveal Contrasting Spatial Structures between Male and Female Aedes aegypti (L.) (Diptera: Culicidae) Populations in Metropolitan Manila, Philippines (2020)
  • Author(s): Carvajal, Thaddeus; Amalin, Divina and Kozo Watanabe
  • Organizer: The Pan-African Mosquito Control Association (PAMCA) - Vectorbase Students and Young Investigators Vector Meeting
  • Int'l Joint Research
• [Presentation] The effect of roads on the population genetic structure of the dengue vector, Aedes aegypti (2020)
  • Author(s): Maria Angenica Fulo Regilme, Thaddeus Carvajal , Ann?Christin Honnen, Divina Amalin, Kozo Watanabe
  • Organizer: 2020 Annual Virtual Meeting of the American Society of Tropical Medicine and Hygiene
  • Int'l Joint Research
• [Presentation] Dengue Disease Dynamics are Modulated by Combinatory Influences of Precipitation and Landscapes: A Machine Learning ? based Approach (2020)
  • Author(s): Micanaldo Ernesto Francisco, Thaddeus M. Carvajal, Masahiro Ryo, Kei Nukazawa, Divina M. Amalin and Kozo Watanabea
  • Organizer: Water and Environment Technology Conference 2020 Online (WET2020-Online)
  • Int'l Joint Research
• [Presentation] The Status of Biological Control Approaches Against Aedes-borne Diseases: How Far Are We in Effectively Controlling this Mosquito Vector? (2020)
  • Author(s): Thaddeus Carvajal, Jayson Capistrano, Phoebe Diez, Paolo Pacheco, Jerica Isabel Reyes, Howell Ho, Divina Amalin and Kozo Watanabe
  • Organizer: Rundown International Webinar: Environmental Health Emerging Diseases
  • Int'l Joint Research / Invited
• [Presentation] "Development and assessment of Mozzify app: an integrated mhealth for real-time Dengue reporting and mapping, health communication and behavior modification reporting and mapping, health communication and behavior modification" (2019)
  • Author(s): Herbuela, Von Ralph Dane M., Karita Tomonori, Francisco, Micanaldo, Watanabe Kozo
  • Organizer: 1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research
• [Presentation] Designing and Developing a Nanobiosensor for the detection of important microbials in Ae. aegypti (2019)
  • Author(s): Carvajal Thadeus, Alocilja Evangeline, Divina Amalin and Watanabe Kozo
  • Organizer: 1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research / Invited
• [Presentation] Detection of Wolbachia in Field-collected Dengue Vector Mosquito, Aedes aegypti in the Philippines (2019)
  • Author(s): Kozo Watanabe
  • Organizer: 1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research / Invited
• [Presentation] A Bioclimate-Based Maximum Entropy Model for Comperiella calauanica Barrion, Almarinez & Amalin (Hymenoptera: Encyrtidae) and Its Utility in Predicting the Occurrence of Aspidiotus rigidus Reyne (Hemiptera: Diaspididae) in the Philippines (2019)
  • Author(s): Almarinez, BLM., Fadri, MJA., Lasina, R., Carvajal, TM., Watanabe, K., Legaspi, JC., and DM Amalin
  • Organizer: 1st JSPS Core-to-Core Symposium: Center of Excellence in Health Risk Assessment for Adaptation to Climate Change
  • Int'l Joint Research
• [Funded Workshop] International Symposium (Webinar) of jointly organized by Ehime University and De La Salle University - Philippines "Usable Science Resulting in Impact" (2020)