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Establishment of the consumer-oriented breeding system in blueberry based on genomic and cognitive sciences

Research Project

Project/Area Number 19KK0156
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionKyoto University

Principal Investigator

TAO RYUTARO  京都大学, 農学研究科, 教授 (10211997)

Co-Investigator(Kenkyū-buntansha) 澤田 玲子  京都大学, 農学研究科, 研究員 (20713043)
西山 総一郎  京都大学, 農学研究科, 助教 (50827566)
山根 久代  京都大学, 農学研究科, 准教授 (80335306)
Project Period (FY) 2019-10-07 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2023: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2022: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2021: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords果樹 / 園芸 / 嗜好性 / 栽培化 / 倍数体
Outline of Research at the Start

本研究では,USDAの保有する多様なサザンハイブッシュブルーベリーの遺伝資源を活用し,高品質品種の開発を加速化するための基盤整備を行う.認知科学の手法を取り入れ,嗜好・分析型の官能評価試験を実施し,高品質果実生産のための品種育成に活用する.また,ゲノムワイドな解析によりサザンハイブッシュブルーベリーの育種成立過程を解明する.さらに果実の生化学的特性に基づいてゲノム多型と嗜好性要因を紐付けする.ゲノムワイドな解析は,開花期など暖地栽培に関連する農業形質の解明にも適用する.以上より,日本独自の高品質ブルーベリー育成のための育種体系を確立し,消費者を指向する暖地適応型ブルーベリー育種に応用する.

Outline of Final Research Achievements

This study is an international collaborative research project between Japan and the United States on blueberry breeding. Utilizing the diverse genetic resources of blueberries, we elucidated the breeding process based on genome-wide polymorphism information. Additionally, metabolite profiling was conducted to obtain information linking genomic polymorphisms with preference factors based on biochemical characteristics. We performed both hedonic sensory evaluations and analytical sensory evaluations, obtaining basic information for the production of high-quality fruits. Consequently, this study gave significant information for establishing breeding strategies and marker-assisted breeding systems that offer the optimal path for developing Japanese original high-quality blueberries. The information obtained can be applied to consumer-oriented breeding of warm-climate-adapted blueberries.

Academic Significance and Societal Importance of the Research Achievements

日本におけるブルーベリー栽培は歴史が浅く,1951年に米国から導入したのがはじまりであり,本格的な栽培は1980年代の後半からである.現在までブルーベリーの消費は増加し続けており,日本の風土や環境条件,消費者嗜好にあった品種の育成は急務である.本研究では,米国で保存されている多様なブルーベリーの遺伝資源を利用して,ゲノム解析,ならびに食味と外観に関する官能評価を行い,ブルーベリーの環境適応性や進化に関する基礎学術知見に加え,果実の嗜好性をゲノミクス,認知科学,生化学の視点から特徴づけたものであり,得られた知見は暖地適応型ブルーベリーの消費者指向型育種戦略の構築に応用することが可能である.

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (26 results)

All 2023 2022 2021 2020

All Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 10 results,  Open Access: 2 results) Presentation (15 results) (of which Int'l Joint Research: 7 results,  Invited: 1 results)

  • [Journal Article] Efficient Transient Expression for Functional Analysis in Fruit Using the Tsukuba System Vector2023

    • Author(s)
      Omori Masafumi、Fujiwara Yosuke、Yamane Hisayo、Miura Kenji、Tao Ryutaro
    • Journal Title

      The Horticulture Journal

      Volume: 92 Issue: 3 Pages: 261-268

    • DOI

      10.2503/hortj.QH-062

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-Wide Identification of Loci Associated With Phenology-Related Traits and Their Adaptive Variations in a Highbush Blueberry Collection2022

    • Author(s)
      Nagasaka Kyoka、Nishiyama Soichiro、Fujikawa Mao、Yamane Hisayo、Shirasawa Kenta、Babiker Ebrahiem、Tao Ryutaro
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 793679-793679

    • DOI

      10.3389/fpls.2021.793679

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insights into the Physiological and Molecular Mechanisms Underlying Highbush Blueberry Fruit Growth Affected by the Pollen Source2022

    • Author(s)
      Nagasaka Kyoka、Yamane Hisayo、Nishiyama Soichiro、Ebihara Shu、Matsuzaki Ryusuke、Shoji Masakazu、Tao Ryutaro
    • Journal Title

      The Horticulture Journal

      Volume: 91 Issue: 2 Pages: 140-151

    • DOI

      10.2503/hortj.UTD-332

    • NAID

      130008142948

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Off-season flowering and expression of flowering-related genes during floral bud differentiation of rabbiteye blueberry in a subtropical climate2022

    • Author(s)
      Omori Masafumi、Cheng Chieh-Chi、Hsu Fu-Chiun、Chen Shiang-Jiuun、Yamane Hisayo、Tao Ryutaro、Li Kuo-Tan
    • Journal Title

      Scientia Horticulturae

      Volume: 306 Pages: 111458-111458

    • DOI

      10.1016/j.scienta.2022.111458

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Epigenetic modification, a novel strategy to artificially control blueberry fruit ripening.2022

    • Author(s)
      Li, T., J. Zhang, H. Yamane and R. Tao.
    • Journal Title

      Acta Hort.

      Volume: 1344 Issue: 1344 Pages: 1-4

    • DOI

      10.17660/actahortic.2022.1344.1

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Preharvest long-term exposure to UV-B radiation promotes fruit ripening and modifies stage-specific anthocyanin metabolism in highbush blueberry2021

    • Author(s)
      Li, T., H. Yamane and R. Tao
    • Journal Title

      Horticulture Research

      Volume: 8 Issue: 1 Pages: 67-67

    • DOI

      10.1038/s41438-021-00503-4

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of preharvest UV-B irradiation on anthocyanin biosynthesis in blueberry2021

    • Author(s)
      Li, T., H. Yamane and R. Tao
    • Journal Title

      Acta Horticulturae

      Volume: 1312 Issue: 1312 Pages: 139-144

    • DOI

      10.17660/actahortic.2021.1312.21

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the effects of pollination on fruit size and quality of highbush blueberry2021

    • Author(s)
      Nagasaka, K., H. Yamane and R. Tao
    • Journal Title

      Acta Hortic.

      Volume: 1312 Issue: 1312 Pages: 25-30

    • DOI

      10.17660/actahortic.2021.1312.4

    • Related Report
      2021 Research-status Report
  • [Journal Article] Genomic insight into the developmental history of southern highbush blueberry populations2020

    • Author(s)
      Nishiyama, S., M. Fujikawa, H. Yamane, K. Shirasawa, E. Babiker and R. Tao
    • Journal Title

      Heredity

      Volume: 126 Issue: 1 Pages: 194-205

    • DOI

      10.1038/s41437-020-00362-0

    • NAID

      120006958094

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Expressional analysis of FT and CEN genes in a continuously flowering highbush blueberry 'Blue Muffin'2020

    • Author(s)
      Omori, M., H. Yamane, K-T. Li, R. Matsuzaki, S. Ebihara, T-S. Li and R. Tao.
    • Journal Title

      Acta Hort.

      Volume: 1280 Issue: 1280 Pages: 197-201

    • DOI

      10.17660/actahortic.2020.1280.27

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Trageted mutagenesis of CENTRORADIALIS using CRISPR/Cas9 system through the improvement of genetic transformation efficiency of tetraploid highbush blueberry2020

    • Author(s)
      Omori, M., H. Yamane, K. Osakabe, Y. Osakabe, and R. Tao
    • Journal Title

      J. Hortic. Sci. Biotec.

      Volume: 96 Issue: 2 Pages: 153-161

    • DOI

      10.1080/14620316.2020.1822760

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] Characterization of ethylene and ABA biosynthesis and response in rabbiteye blueberry ‘Tifblue’2023

    • Author(s)
      Bao, Y., H. Yamane, R. Nakano, M. Gao-Takai and R. Tao
    • Organizer
      Asian Horticulture Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゲノム編集によるブルーベリー早期開花系統の作出2023

    • Author(s)
      大森真史*・山根久代・刑部祐里子・刑部敬史・田尾龍太郎
    • Organizer
      園芸学会令和5年度春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Epigenetic modification, a novel strategy to artificially control blueberry fruit ripening2022

    • Author(s)
      Li, T., H. Yamane, and R. Tao
    • Organizer
      ISHS XIV International Symposium on Plant Bioregulators in Fruit Production
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Polyploid GWAS identifies loci associated with phenology-related traits in southern highbush blueberry2022

    • Author(s)
      Nagasaka, K., S. Nishiyama, M. Fujikawa, H. Yamane, K. Shirasawa, E. Babiker, R. Tao
    • Organizer
      31st International Horticultural Congress
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Spatiotemporal genome-wide DNA methylation changes during blueberry ripening and DNA demethylation-induced cluster ripening2022

    • Author(s)
      Li, T., H. Yamane and R. Tao
    • Organizer
      31st International Horticultural Congress
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 内在性プロモーターを用いたブルーベリーにおけるゲノム編集効率の向上2022

    • Author(s)
      大森真史・山根久代・刑部祐里子・刑部敬史・田尾龍太郎
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ブルーベリーの味および食感特性が果実の嗜好性に与える影響2022

    • Author(s)
      平岩尚樹・山根久代・西山総一郎・田尾龍太郎
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ブルーベリー再分化メカニズムに関するトランスクリプトーム解析2022

    • Author(s)
      大森真史・山根久代・田尾龍太郎
    • Organizer
      園芸学会令和4年度秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 倍数体モデルに基づくサザンハイブッシュブルーベリーのゲノムワイドアソシエーション解析2021

    • Author(s)
      長坂京香,西山総一郎,藤川真央,山根久代,白澤健太,E. Babiker,田尾龍太郎
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] CRISPR/Cas9によるハイブッシュブルーベリーのゲノム編集2021

    • Author(s)
      大森真史,山根久代,刑部祐里子,刑部敬史,田尾龍太郎
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Effects of pre-harvest UV-B irradiation on anthocyanin biosynthesis in blueberry2020

    • Author(s)
      Li, T., H. Yamane and R. Tao
    • Organizer
      3rd Asian Horticulture Congress
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Evaluation of the effects of pollination on fruit size and quality of highbush blueberry2020

    • Author(s)
      Yamane, H., K. Nagasaka, S. Ebihara, T. Li, R. Matsuzaki, M. Shoji and R. Tao
    • Organizer
      3rd Asian Horticulture Congress
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] サザンハイブッシュブルーベリーにおける重要形質のゲノムワイドアソシエーション解析2020

    • Author(s)
      西山総一郎,藤川真央,山根久代,白澤健太,E. Babiker,田尾龍太郎
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] サザンハイブッシュブルーベリーにおける重要形質のゲノムワイドアソシエーション解析2020

    • Author(s)
      西山総一郎*・藤川真央・山根久代・白澤健太・E. Babiker・田尾龍太郎
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Towards understanding of genome evolution of southern highbush blueberry by ddRAD-Seq2020

    • Author(s)
      Nishiyama*, S., M. Fujikawa, E. Babiker, H. Yamane, K. Shirasawa and R. Tao
    • Organizer
      Plant and Animal Genome Conference XXVII
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-10-10   Modified: 2025-01-30  

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