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Studies on development of control strategy for Asian zoonotic schistosomiasis with microsatellite marker system

Research Project

Project/Area Number 19KK0173
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 42:Veterinary medical science, animal science, and related fields
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

KAWAZU Shin-ichiro  帯広畜産大学, 原虫病研究センター, 教授 (60312295)

Co-Investigator(Kenkyū-buntansha) 尾針 由真  酪農学園大学, 獣医学群, 助教 (00847056)
桐木 雅史  獨協医科大学, 医学部, 講師 (50265302)
Project Period (FY) 2019-10-07 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2022: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords日本住血吸虫症 / 顧みられない熱帯病 / 人獣共通感染症 / スイギュウ / マイクロサテライトマーカー
Outline of Research at the Start

日本住血吸虫症はアジアの農村や漁村で流行し、家畜動物から中間宿主貝を介してヒトへの感染も成立することから、保健衛生および家畜衛生と密接に関連した顧みられない人獣共通感染症となっている。日本住血吸虫症の排除(elimination)を達成するには、寄生虫のライフサイクルを俯瞰的に把握する必要がある。本研究では、島嶼国フィリピンの多様な寄生虫ライフサイクル全体を対象としたマイクロサテライト(STR)マーカーによる多座位の遺伝子型(MLG)解析解析から、各宿主を嗜好して適応した寄生虫集団の存在を証明してその遺伝的特性(マーカー型)を明らかにする。同時にマーカーと患者での病態との関係も明らかにする。

Outline of Final Research Achievements

To prepare DNA from single parasite egg for multi-locus genotyping using microsatellite markers (STR/MLG), the current protocol of Miracidium Hatching Test (MHT) was modified. Two surveys were conducted in 2022 and 2023 in near the city of Tacloban, Leyte province, where the parasite eggs were collected from patients and water buffaloes. STR/MLG with the single-egg DNA samples showed gene flow among parasites distributed in these villages, suggesting zoonotic lifecycle of the parasite and human and livestock migration in the area. Serodiagnostic means needed in surveillance for schistosomiasis mekongi, which is endemic in Mekong Delta (Lao PDR and Cambodia), were examined. An ELISA using a recombinant protein (rSmekTPx-1) as antigen was validated and showed satisfied sensitivity and specificity, although cross-reactivity with some sera of opisthorchiasis patients was observed.

Academic Significance and Societal Importance of the Research Achievements

日本住血吸虫の集団構造解析に使用する単一寄生虫卵由来のDNAサンプルを調整するため、現行のミラシジウム孵化法(MTA)に改良を加えたプロトコールを確立した。MHTの改良法を日本住血吸虫症の対策に導入することで、流行地での寄生虫の集団構造解析が可能になる。また、メコン住血吸虫症の調査研究において必要になる、血清診断法(酵素抗体法:ELISA)のプロトコールを検討した。組換体抗原を用いるELISAは、抗原の大量供給と品質管理が容易なことから、大規模調査への応用が期待できる。これらの手法の導入により、アジア型住血吸虫症の監視と排除に向けた寄生虫病対策の更なる進展が期待できる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (44 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (14 results) Journal Article (10 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 10 results,  Open Access: 4 results) Presentation (16 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results) Remarks (4 results)

  • [Int'l Joint Research] フィリピン大学マニラ校/フィリピンカラバオセンター(フィリピン)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] カンボジア保健省国立マラリアセンター(カンボジア)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ラオス熱帯医学公衆衛生研究所(ラオス)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] WHO東南アジア地域事務局 (SEARO)(インド)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] フィリピン大学マニラ校/フィリピンカラバオセンター/WHO西太平洋地域事務局(WPRO)(フィリピン)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] カンボジア保健省国立マラリア センター(カンボジア)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] ラオス熱帯医学公衆衛生研究所(ラオス)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] WHO東南アジア地域事務局(SEARO)(インド)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] リリピン大学マニラ校/フィリピンカラバオセンター/WHO西太平洋事務局(WPRO)(フィリピン)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] カンボジア保健省国立マラリアセンター(カンボジア)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] ラオス熱帯医学公衆衛生研究所(ラオス)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] フィリピン大学マニラ校公衆衛 生学部/カビテ州立大学獣医科学部/フィリピンカラバオセンター(フィリピン)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] NIMPE, Ho Chi Minh City(ベトナム)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] フィリピン大学マニラ校公衆衛生学部/カビテ州立大学獣医科学部/フィリピンカラバオセンター(フィリピン)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Expression profile analysis of the transient receptor potential (TRPM) channel, a possible target of praziquantel in Schistosoma japonicum.2023

    • Author(s)
      Shinozaki, K., Kirinoki, M., Atcharaphan, W., Watanabe, KI., Ohari, Y., Suguta, S., Ona, KAL., Ushio, N., Macalanda, AMC., Suganuma, K., Inoue, N., and Kawazu, SI.
    • Journal Title

      Parasitol. Int.

      Volume: 99 Pages: 102833-102833

    • DOI

      10.1016/j.parint.2023.102833

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cloning, expression and evaluation of thioredoxin peroxidase-1 antigen for the serological diagnosis of Schistosoma mekongi human infection.2023

    • Author(s)
      Wanlop, A., Angeles, JMM., Macalanda, AMC., Kirinoki, M., Ohari, Y., Yajima, A., Yamagishi, J., Ona, KAL., and Kawazu, S.
    • Journal Title

      Diagnostics (Basel)

      Volume: 12(12) Issue: 12 Pages: 3077-3077

    • DOI

      10.3390/diagnostics12123077

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of crude and recombinant antigens of Schistosoma japonicum for the detection of Schistosoma mekongi human infection.2023

    • Author(s)
      Angeles, JMM., Wanlop, A., Dang-Trinh MA., Kirinoki, M., Kawazu, S., and Yajima, A.
    • Journal Title

      Diagnostics (Basel)

      Volume: 13(2) Issue: 2 Pages: 184-184

    • DOI

      10.3390/diagnostics13020184

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Serological evaluation of the schistosome’s secretory enzyme phytochelatin synthase and phosphoglycerate mutase for the detection of human Schistosoma japonicum infection.2022

    • Author(s)
      Angeles, JMM., Goto, Y., Dang-Trinh MA., Rivera, PT., Villacorte, EA., and Kawazu, S.
    • Journal Title

      Parasitol. Res.

      Volume: 121(8) Issue: 8 Pages: 2445-2448

    • DOI

      10.1007/s00436-022-07568-7

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A simple and efficient miracidium hatching technique for preparing a single-genome DNA sample of <i>Schistosoma japonicum</i>2022

    • Author(s)
      Wanlop, A., Dang-Trinh, MA., Kirinoki, M., Suguta, S., Shinozaki, K., and Kawazu, S.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 84 Issue: 8 Pages: 1108-1110

    • DOI

      10.1292/jvms.21-0536

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Field evaluation of recombinant antigen ELISA in detecting zoonotic schistosome infection among water buffaloes in endemic municipalities in the Philippines.2020

    • Author(s)
      Angeles, JMM., Goto, Y., Kirinoki, M., Villacorte, EA., Moendeg, KJ., Rivera, PT., Chigusa, Y., and Kawazu, S.
    • Journal Title

      Fronters in Veterinary Sciences

      Volume: 7 Pages: 592783-592783

    • DOI

      10.3389/fvets.2020.592783

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analyses of the expression, immunohistochemical properties and serodiagnostic potential of Schistosoma japonicum peroxiredoxin-4.2020

    • Author(s)
      Dang-Trinh MA., Angeles, JMM., Moendeg, KJ., Macalanda, AMC., Nguyen, TT., Higuchi, L., Nakagun, S., Kirinoki, M., Chigusa, Y., Goto, Y., and Kawazu, S.
    • Journal Title

      Parasites & Vectors

      Volume: 13 Issue: 1 Pages: 436-436

    • DOI

      10.1186/s13071-020-04313-w

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of canine <i>Schistosoma japonicum</i> infection using recombinant thioredoxin peroxidase-1 and tandem repeat proteins2019

    • Author(s)
      Angeles, JMM., Goto, Y., Kirinoki, M., Leonardo, LR., Moendeg, KJ., Ybanez AP., Rivera, PT., Villacorte, EA., Inoue, N., Chigusa, Y., and Kawazu, S.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 10 Pages: 1413-1418

    • DOI

      10.1292/jvms.19-0126

    • NAID

      130007730600

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Schistosoma japonicum cathepsin B as potential diagnostic antigen for Asian zoonotic schistosomiasis.2019

    • Author(s)
      Macalanda, AMC., Angeles, JMM., Moendeg, KJ., Dang-Trinh, MA., Higuchi, L., Kirinoki, M., Chigusa, Y., Leonardo, LR., Villacorte, EA., Rivera, PT., Goto, Y., and Kawazu, S.
    • Journal Title

      Parasitology Research

      Volume: 118 Issue: 9 Pages: 2601-2608

    • DOI

      10.1007/s00436-019-06410-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Infection rate of Schistosoma japonicum in the snail Oncomelania hupensis quadrasi in endemic villages in the Philippines: Need for snail surveillance technique.2019

    • Author(s)
      Fornillos, RJC., Fontanilla, IKC., Chigusa, Y., Kikuchi, M., Kirinoki, M., Kato-Hayashi, N., Kawazu, S., Angeles, JM., Tabios, IK., Moendeg, K., Goto, Y., Tamayo, PG., Gampoy, EF., Pates, I., Chua, JC., and Leonardo, LR.
    • Journal Title

      Tropical Biomedicine

      Volume: 36 Pages: 402-411

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] プラジカンテル標的候補分子TRPチャネルの日本住血吸虫における発現プロファイル解析2024

    • Author(s)
      篠崎夏歩、桐木雅史、Wanlop Atcharaphan、渡邉謙一、尾針由真、Kevin Ona、潮奈々子、Adrian Macaranda、菅沼啓輔、井上昇、河津信一郎
    • Organizer
      第93回日本寄生虫学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] マンソン住血吸虫sulfite oxidaseの酵素学的特徴の解明2023

    • Author(s)
      直田紗希、Acharjee Rajib、林下瑞希、佐倉孝哉、濱野真二郎、北潔、ダニエル健稲岡、菅沼啓輔、井上昇、河津信一郎
    • Organizer
      第166回日本獣医学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Schistosoma mekongi TPx-1 as ELISA antigen for diagnosis of Asian schistosomiasis.2023

    • Author(s)
      Wanlop A., Angeles JMA., Kirinoki M., Dang TMA., Ohari, Y., Yamagishi J., Yajima A., and Kawazu S.
    • Organizer
      2023年日米医学合同EID会議 寄生虫疾患部会パネル会議 Manila, Philippines(シンポジウム 口頭発表
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Diagnostic performance of recombinant antigen thioredoxin peroxidase-1 (TPx-1) in detection of Schistosoma mekongi infection.2023

    • Author(s)
      Wanlop A., Angeles JMA., Kirinoki M., Dang, TMA., Ohari Y., Yagagishi J., Yajima A., and Kawazu S.
    • Organizer
      第92回日本寄生虫学会大会 金沢市(口演発表)
    • Related Report
      2022 Research-status Report
  • [Presentation] Diagnostic potential of Schistosoma japonicum recombinant antigens with ELISA in detecting S. mekongi human infection in Laos.2022

    • Author(s)
      Wanlop A., Angeles JMA., Kirinoki M., Dang, TMA., Yajima A., and Kawazu S.
    • Organizer
      第91回日本寄生虫学会大会 帯広市(ポスター発表)
    • Related Report
      2022 Research-status Report
  • [Presentation] Expression and evaluation of recombinant antigen thioredoxin peroxidase-1 (TPx-1) from Schistosoma mekongi for schistosomiasis diagnosis.2022

    • Author(s)
      Wanlop A., Angeles JMA., Kirinoki M., Dang, TMA., Yajima A., and Kawazu S.
    • Organizer
      2022国際熱帯医学・マラリア会議(ICTM2022)Bangkok, Thailand(口頭発表)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] The expression profile analysis of transient receptor potential (TRP) channel, the possible target of praziquantel in Schistosoma japonicum.2022

    • Author(s)
      Shinozaki K., Kirinoki M., Wanlop A., Watanabe, K., Ohari, Y., Suguta, S., Suganuma, K., Inoue, N., and Kawazu S.
    • Organizer
      2022米国熱帯医学会年会 Seattle, Washington USA(ポスター発表)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of Schistosoma japonicum peroxiredoxin-4 and evaluation of its serodiagnostic potential as an antigen against human schistosomiasis.2021

    • Author(s)
      Dang TMA., Angeles JMA., Moendeg KJ., Macalanda AMC., Ohari Y., Wanlop A., Kirinoki M., Chigusa Y., Goto Y., and Kawazu S.
    • Organizer
      第90回日本寄生虫学会大会(ハイブリッド集会)口頭発表 2021年4月17日 奈良市
    • Related Report
      2021 Research-status Report
  • [Presentation] Field evaluation of recombinant antigen ELISA for the detection of Schistosoma japonicum infection among water buffaloes in endemic municipalities in the Philippines.2021

    • Author(s)
      Angeles JMA., Goto Y., Kirinoki M., Villacorte, E., Rivera, P., Chigusa, Y., and Kawazu S.
    • Organizer
      第90回日本寄生虫学会大会(ハイブリッド集会)口頭発表 2021年4月17日 奈良市
    • Related Report
      2021 Research-status Report
  • [Presentation] Evaluation of recombinant Schistosoma japonicum antigens for the detection of S. mekongi human infection.2021

    • Author(s)
      Wanlop A., Angeles JMA., Kirinoki M., Dang TMA., Yajima, A., and Kawazu, S.
    • Organizer
      第164回日本獣医学会学術集会(オンライン集会)口頭発表 2021年9月8日 江別市
    • Related Report
      2021 Research-status Report
  • [Presentation] Application of multi-epitope recombinant proteins for serodiagnosis of Asian zoonotic schistosomiasis.2020

    • Author(s)
      Dang TMA., Angeles JMA., Moendeg KJ., Macalanda AMC., Ohari Y., Wanlop A., Kirinoki M., Chigusa Y., Goto Y., and Kawazu S.
    • Organizer
      第89回日本寄生虫学会大会 ポスター発表(集会中止・誌上発表)
    • Related Report
      2020 Research-status Report
  • [Presentation] ラオス国チャンパサック県における血清診断によるメコン住血吸虫症の流行状況と、組換えタンパク質を用いたELISA法の検討2020

    • Author(s)
      熊谷 貴、須賀愛希、Sengdeuane K、Phonepadith K、Lavy L、Pheovaly S、Bouasy H、河津信一郎、岩永史朗、Paul TB、狩野繁之、石上盛敏
    • Organizer
      第89回日本寄生虫学会大会 ポスター発表(集会中止・誌上発表)
    • Related Report
      2020 Research-status Report
  • [Presentation] Japan-Philippines Academe Partnership for Schistosomiasis Control.2020

    • Author(s)
      Angeles JMA., Kirinoki M., Goto Y., and Kawazu S.
    • Organizer
      SCH Stakeholder's Forum. (Zoom meeting) シンポジウム(招待講演)
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Expression, immunohistochemical analysis and evaluation of serodiagnostic potential of Schistosoma japonicum Peroxiredoxin-4.2019

    • Author(s)
      Dang TMA., Angeles JMA., Moendeg KJ., Macalanda AMC., Nguyen TT., Higuchi L., Nakagun S., Kirinoki M., Chigusa Y., Goto Y., and Kawazu S.
    • Organizer
      第60回日本熱帯医学会大会 ワークショップ
    • Related Report
      2019 Research-status Report
  • [Presentation] 住血吸虫症対策におけるReservoir Hostの検査法の開発‐フィリピンにおける住血吸虫症対策での経験から2019

    • Author(s)
      河津信一郎
    • Organizer
      NTD’s研究集会 シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Evaluation of Schistosoma japonicum Peroxiredoxin-4 for its serodiagnostic potential.2019

    • Author(s)
      Dang TMA., Angeles JMA., Kirinoki M., Chigusa Y., Goto Y., and Kawazu S.
    • Organizer
      日米医学寄生虫疾患部会合同会議 第50回日米合同会議
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 帯広畜産大学 原虫病研究センター (日本語版)

    • URL

      https://www.obihiro.ac.jp/facility/protozoa/

    • Related Report
      2023 Annual Research Report 2022 Research-status Report 2021 Research-status Report 2020 Research-status Report
  • [Remarks] 帯広畜産大学 原虫病研究センター (英語版)

    • URL

      https://www.obihiro.ac.jp/facility/protozoa/en

    • Related Report
      2023 Annual Research Report 2022 Research-status Report 2021 Research-status Report 2020 Research-status Report
  • [Remarks] 帯広畜産大学 原虫病研究センター(日本語版)

    • URL

      https://www.obihiro.ac.jp/facility/protozoa/

    • Related Report
      2019 Research-status Report
  • [Remarks] 帯広畜産大学 原虫病研究センター(英語版)

    • Related Report
      2019 Research-status Report

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Published: 2019-10-10   Modified: 2025-01-30  

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