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Eco-epidemiological Survey of Canine Filariasis in Thailand Toward the Creation of Filaria-free Mosquitoes

Research Project

Project/Area Number 19KK0175
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 42:Veterinary medical science, animal science, and related fields
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

Fukumoto Shinya  帯広畜産大学, 原虫病研究センター, 准教授 (50376422)

Co-Investigator(Kenkyū-buntansha) 茅野 光範  帯広畜産大学, 畜産学部, 准教授 (20590095)
曽賀 晃  帯広畜産大学, 原虫病研究センター, 共同研究員 (20838734)
白水 貴大  藤田医科大学, 病態モデル先端医学研究センター, 助教 (80804608)
Project Period (FY) 2019-10-07 – 2025-03-31
Project Status Completed (Fiscal Year 2024)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2023: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2022: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords犬糸状虫 / フィラリア / ヤブカ / イヌ糸状虫 / ベクター
Outline of Research at the Start

蚊は病原体の媒介者として、人類に最も脅威を与えている生物(Top Deadliest Animal)である。殺虫剤耐性、生態系への影響などへの問題から、殺虫剤による蚊の撲滅は困難である。そこで近年、病原体を媒介しない蚊へと置換することで、感染症を制圧できないかとの概念が浮上してきた。近年のゲノム編集技術の進歩により、病原体を媒介しない蚊の実現が技術的に可能になってきた。そこで本研究では、犬糸状虫を媒介しない蚊の作出実現にむけた基礎的知見を得るために、タイ王国で犬と蚊における犬糸状虫症疫学調査を行い、どのような遺伝子応答が蚊による犬糸状虫の媒介に重要なのかフィールドレベルで解析を行う。

Outline of Final Research Achievements

This project aims to develop a mosquito that does not transmit filaria. We have succeeded in colonizing the Aedes mosquito, a canine filarial vector, collected in Thailand, and have used genetic methods to generate a mosquito that does not transmit canine filariasis from this colony. We also estimated the factors related to filarial resistance by genome analysis. This research has laid the foundation for controlling filaria by pathogen-free mosquitoes and is expected to contribute to the future elimination of filaria.

Academic Significance and Societal Importance of the Research Achievements

地球上で最も人類に脅威を与えている生物は蚊である。種々の病原体を媒介することで医学・獣医学上の重要な懸念となっている。近年、病原体を媒介しない蚊を用いた感染症対策に注目が集まっている。本課題では、タイ王国でのフィールド研究を通じて、フィールドの蚊から、フィラリアを媒介しない蚊を遺伝学的に作出することに成功した。すなわち、本研究により病原体を媒介しない蚊によるフィラリア制御の基盤構築に成功した。

Report

(7 results)
  • 2024 Annual Research Report   Final Research Report ( PDF )
  • 2023 Research-status Report
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (38 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (5 results) Journal Article (13 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 13 results,  Open Access: 8 results) Presentation (20 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] チェンマイ大学(タイ)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] チェンマイ大学/プリンスオブソンクラー大学(タイ)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] モンゴル生命科学大学(モンゴル)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] チェンマイ大学/プリンスオブソンクラー大学(タイ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] チェンマイ大学/プリンスオブソンクラー大学(タイ)

    • Related Report
      2019 Research-status Report
  • [Journal Article] High pyrethroid resistance is associated with high frequencies of 1014F and 1014S kdr mutations in <i>Anopheles arabiensis</i> (Diptera: Culicidae) from Ouagadougou, Burkina Faso2024

    • Author(s)
      Somda Zephirin、Zanre Nicolas、Wangrawa Dimitri W、Toe Hyacinthe K、Sombie Aboubacar、Saiki Erisha、Fukumoto Shinya、Sakurai Tatsuya、Sanon Antoine、McCall Philip J、Kanuka Hirotaka、Weetman David、Badolo Athanase
    • Journal Title

      Journal of Medical Entomology

      Volume: 62 Issue: 2 Pages: 381-388

    • DOI

      10.1093/jme/tjae135

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Wolbachia infection in Aedes aegypti does not affect its vectorial capacity for Dirofilaria immitis2024

    • Author(s)
      Shirozu Takahiro、Regilme Maria Angenica F.、Ote Manabu、Sasaki Mizuki、Soga Akira、Bochimoto Hiroki、Kawabata Hidenobu、Umemiya-Shirafuji Rika、Kanuka Hirotaka、Fukumoto Shinya
    • Journal Title

      Scientific Reports

      Volume: 14 Issue: 1 Pages: 22528-22528

    • DOI

      10.1038/s41598-024-73421-9

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a novel rodent model for dog heartworm microfilaremia using the severe-combined immunodeficiency mouse2024

    • Author(s)
      Mizuseki Mihoko、Ikeda Nao、Shirozu Takahiro、Yamagishi Maki、Oshiro Sugao、Fukumoto Shinya
    • Journal Title

      Scientific Reports

      Volume: 14 Issue: 1 Pages: 13741-13741

    • DOI

      10.1038/s41598-024-63165-x

    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Searching for new molecules involved in <i>Anopheles</i> mosquitoes’ response to <i>Plasmodium</i> infection2024

    • Author(s)
      HARAGUCHI Asako、TAKANO Makoto、FUJIWARA Kanta、HAKOZAKI Jun、NAKAYAMA Kazuhiko、NAKAMURA Sakure、YOSHIKAWA Yasunaga、FUKUMOTO Shinya、KUSAKISAKO Kodai、IKADAI Hiromi
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 86 Issue: 5 Pages: 485-492

    • DOI

      10.1292/jvms.24-0008

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2024 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serological survey of Toxoplasma gondii infection in endangered Iriomote cat (Prionailurus bengalensis iriomotensis) and simulation of the effect on population dynamics2023

    • Author(s)
      Shirozu Takahiro、Kayano Mitsunori、Hirose Fuyuko、Oshiro Sugao、Nagamine Takashi、Endo Yasuyuki、Izawa Masako、Xuan Xuenan、Fukumoto Shinya
    • Journal Title

      European Journal of Wildlife Research

      Volume: 69 Issue: 4 Pages: 70-70

    • DOI

      10.1007/s10344-023-01702-1

    • Related Report
      2023 Research-status Report
    • Peer Reviewed
  • [Journal Article] Challenges for Precise Subtyping and Sequencing of a H5N1 Clade 2.3.4.4b Highly Pathogenic Avian Influenza Virus Isolated in Japan in the 2022?2023 Season Using Classical Serological and Molecular Methods2023

    • Author(s)
      Komu James G.、Nguyen Hiep Dinh、Takeda Yohei、Fukumoto Shinya、Imai Kunitoshi、Takemae Hitoshi、Mizutani Tetsuya、Ogawa Haruko
    • Journal Title

      Viruses

      Volume: 15 Issue: 11 Pages: 2274-2274

    • DOI

      10.3390/v15112274

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Seasonal Variation and Factors Affecting Trypanosoma theileri Infection in Wild Sika Deer (Ezo Sika Deer Cervus nippon yesoensis) in Eastern Hokkaido2023

    • Author(s)
      Hong Yujon、Suganuma Keisuke、Ohari Yuma、Kayano Mitsunori、Nakazaki Kenji、Fukumoto Shinya、Kawazu Shin-ichiro、Inoue Noboru
    • Journal Title

      Animals

      Volume: 13 Issue: 10 Pages: 1707-1707

    • DOI

      10.3390/ani13101707

    • Related Report
      2023 Research-status Report
    • Peer Reviewed
  • [Journal Article] Formation of free oocysts in <i>Anopheles</i> mosquitoes injected with <i>Plasmodium</i> ookinetes2023

    • Author(s)
      HARAGUCHI Asako、TAKANO Makoto、HAKOZAKI Jun、NAKAYAMA Kazuhiko、NAKAMURA Sakure、YOSHIKAWA Yasunaga、FUKUMOTO Shinya、KUSAKISAKO Kodai、IKADAI Hiromi
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 85 Issue: 9 Pages: 921-928

    • DOI

      10.1292/jvms.23-0099

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Generation of Transgenic Mosquitoes Harboring a Replication-Restricted Virus2022

    • Author(s)
      Shinzawa Naoaki、Kashima Chisako、Aonuma Hiroka、Takahashi Kei、Shimojima Masayuki、Fukumoto Shinya、Saiki Erisha、Yamamoto Daisuke S.、Yoshida Shigeto、Matsuoka Hiroyuki、Kawaoka Yoshihiro、Kanuka Hirotaka
    • Journal Title

      Frontiers in Tropical Diseases

      Volume: 3 Pages: 850111-850111

    • DOI

      10.3389/fitd.2022.850111

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia2021

    • Author(s)
      Bilegjargal Janchivsengee、Rzad Izabella、Fukumoto Shinya、Chinchuluun Boldbaatar、Lkhagvatseren Sukhbaatar、Gantuya Sambuu、Azjargal Gansukh、Batsukh Zayat、Munkhjargal Tserendorj
    • Journal Title

      Parasitology International

      Volume: 84 Pages: 102404-102404

    • DOI

      10.1016/j.parint.2021.102404

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Surveillance of canine filarial infection in Ulaanbaatar city2021

    • Author(s)
      Jambaldorj Ankhbayar、Fukumoto Shinya、Tserendorj Munkhjargal
    • Journal Title

      Mongolian Journal of Agricultural Sciences

      Volume: 32 Issue: 1 Pages: 1-4

    • DOI

      10.5564/mjas.v32i1.1593

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification and validation of a commercial cryopreservation medium for the practical preservation of Dirofilaria immitis microfilaria2020

    • Author(s)
      Shirozu Takahiro、Soga Akira、Fukumoto Shinya
    • Journal Title

      Parasites & Vectors

      Volume: 13 Issue: 1 Pages: 383-383

    • DOI

      10.1186/s13071-020-04257-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Geographic Spatial Distribution Patterns of Dirofilaria immitis and Brugia pahangi Infection in Community Dogs in Chiang Mai, Thailand2020

    • Author(s)
      Kaikuntod Manusvee、Arjkumpa Orapun、Kladkempetch Doolyawat、Fukumoto Shinya、Thongkorn Kriangkrai、Boonyapakorn Chavalit、Punyapornwithaya Veerasak、Tiwananthagorn Saruda
    • Journal Title

      Animals

      Volume: 11 Issue: 1 Pages: 33-33

    • DOI

      10.3390/ani11010033

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ネッタイシマカ Liverpool 系統および野生由来近交系を用いた犬糸状虫媒介能に関する QTL 解析2024

    • Author(s)
      白水貴大、佐々木瑞希、Tiwananthagorn, Saruda、福本晋也
    • Organizer
      第76回日本衛生動物学会大会
    • Related Report
      2024 Annual Research Report
  • [Presentation] ネッタイシマカにおけるHT115大腸菌dsRNA発現系を用いたRNAi法の検討2024

    • Author(s)
      片山 菜月、白水 貴大、福本 晋也
    • Organizer
      第167回日本獣医学会学術集会
    • Related Report
      2024 Annual Research Report
  • [Presentation] 北海道に生息するゼニガタアザラシのフィラリア感染疫学調査2024

    • Author(s)
      小島朱吏、末永羅綺、片山菜月、佐々木瑞希、小林万里、福本晋也
    • Organizer
      第70回日本寄生虫学会衛生動物学会合同北日本支部会
    • Related Report
      2024 Annual Research Report
  • [Presentation] Genetic variability analysis of dog heartworm infection phenotypes in Aedes aegypti mosquitoes collected in Chiang Mai2024

    • Author(s)
      Shinya Fukumoto, Takahiro Shirozu, Mizuki Sasaki, Saruda Tiwananthagorn
    • Organizer
      ISAH International Conference 2024
    • Related Report
      2024 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ネッタイシマカのイヌ糸状虫感染表現型に関する遺伝学的個体差の解析2024

    • Author(s)
      白水 貴大、佐々木 瑞希、Tiwananthagorn Saruda、福本 晋也
    • Organizer
      日本昆虫学会第84回大会・第68回日本応用動物昆虫学会大会
    • Related Report
      2023 Research-status Report
  • [Presentation] Development of Epitope-Lacking Transgenic Rodent Malaria Parasite as an Approch for Assessing Vaccine Mechanism of Protection2024

    • Author(s)
      Rasyid Sani Hadiyan、村井 駿介、奥山 竣介、吉田 涼翔、水野 哲志、山本 祐太朗、伊從 光洋、福本 晋也、水上 浩明、志田 壽利、吉田 栄人
    • Organizer
      第93回日本寄生虫学会大会
    • Related Report
      2023 Research-status Report
  • [Presentation] ボルバキア-デング熱対策におけるイヌ糸状虫感染干渉の解析2024

    • Author(s)
      白水 貴大、佐々木 瑞希、大手 学、暮地本 宙己、白藤(梅宮)梨可、嘉糠 洋陸、福本 晋也
    • Organizer
      第93回日本寄生虫学会大会
    • Related Report
      2023 Research-status Report
  • [Presentation] 沖縄県名護市における蚊の捕獲調査および犬糸状虫感染感受性の解析2023

    • Author(s)
      片山 菜月、池田 奈央、水関 実法子、白水 貴大、 福本 晋也
    • Organizer
      第 69 回 日本寄生虫学会・日本衛生動物学会北日本支部合同大会
    • Related Report
      2023 Research-status Report
  • [Presentation] 北海道における鳥類住血胞子虫感染状況の調査 十勝のエゾライチョウから検出されたLeucocytozoon spp.はスウェーデンで検出された系統と一致した2023

    • Author(s)
      橋爪 宏、福本 晋也、纐纈 摩美、佐々木 瑞希
    • Organizer
      第166回日本獣医学会学術集会
    • Related Report
      2023 Research-status Report
  • [Presentation] ハマダラカのマラリア原虫感染に対する新たな応答分子の探索2023

    • Author(s)
      原口麻子、高野真、箱崎純、中山和彦、中村咲蓮、吉川泰永、福本晋也、草木迫 浩大、筏井宏実
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Research-status Report
  • [Presentation] Protective efficacy of viral-vectored P. falciparum vaccine against mutated parasites in a murine model2023

    • Author(s)
      Shunsuke Murai, Yutaro Yamamoto, Shunsuke Okuyama, Wakaba Kanayama, Tetushi Mizuno, Akihiko Sakamoto, Mitsuhiro Iyori, Shinya Fukumoto, Shigeto Yoshida
    • Organizer
      EMBL Conference, BioMalPar XVIII: biology and pathology of the malaria parasite
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research
  • [Presentation] Humoral immune responses to the central repeat region of PfCSP induced by a vial-vectored plasmodium falciparum vaccine play critical roles in protection in a murine model2023

    • Author(s)
      Shunsuke Okuyama, Yutaro Yamamoto, Shunsuke Murai, Mitsuhiro Iyori, Tetsushi Mizuno, Akihiko Sakamoto, Shinya Fukumoto, Hiroaki Mizukami, Hisatoshi Shida, Shigeto Yoshida
    • Organizer
      72nd American Society of Tropical Medicine and Hygiene (ASTMH) Annual Meeting
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research
  • [Presentation] SCID マウスによる犬糸状虫ミクロフィラリア感染モデルの検証2022

    • Author(s)
      水関 実法子,池田 奈央,白水 貴大,福本 晋也
    • Organizer
      第91回日本寄生虫学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 次世代シーケンサーによる犬糸状虫発育ステージ別の転写比較解析2022

    • Author(s)
      池田 奈央、福本晋也
    • Organizer
      第165回日本獣医学会学術集会
    • Related Report
      2022 Research-status Report
  • [Presentation] タイ北部チェンマイ由来ネッタイシマカからの犬糸状虫感受性および抵抗性系統の作出2021

    • Author(s)
      白水貴大、Manusvee Kaiduntod、Doolyawat Kladkempetch, Saruda Tiwananthagorn, 福本晋也
    • Organizer
      第73回日本衛生動物学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 病原体を媒介しない蚊による犬糸状虫症制御2021

    • Author(s)
      福本晋也、白水貴大
    • Organizer
      第73回日本衛生動物学会大会 病害動物の生理分子生物談話会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] フィールド由来ネッタイシマカからの犬糸状虫感受性・抵抗性系統の遺伝学的分離法の確立2021

    • Author(s)
      白水 貴大、Manusvee Kaikuntod、Doolyawat Kladkempetch、Saruda Tiwananthagorn、福本 晋也
    • Organizer
      第166回日本獣医学会学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] Dirofilaria immitis 高媒介および非媒介Aedes aegypti株の交雑後代における感染表現型解析2020

    • Author(s)
      白水貴大、曽賀晃、纐纈摩美、福本晋也
    • Organizer
      第72回日本衛生動物学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 市販細胞凍結保存培地による犬糸状虫ミクロフィラリア凍結保存2020

    • Author(s)
      白水 貴大、福本 晋也
    • Organizer
      第163回日本獣医学会学術集会
    • Related Report
      2020 Research-status Report
  • [Presentation] ネッタイシマカにおける遺伝的性決定随伴したDirofilaria immitis感染抵抗性表現型2019

    • Author(s)
      白水貴大、関 信彰、曽賀 晃、森下雄貴、纐纈摩美、福本晋也
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report

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Published: 2019-10-10   Modified: 2026-01-16  

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