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Evolutionary mechnisms for thermal niche shift in Anolis lizards

Research Project

Project/Area Number 19KK0184
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionTohoku University

Principal Investigator

Kawata Masakado  東北大学, 生命科学研究科, 教授 (90204734)

Co-Investigator(Kenkyū-buntansha) 赤司 寛志  東京理科大学, 先進工学部生命システム工学科, 研究員 (00808644)
牧野 能士  東北大学, 生命科学研究科, 教授 (20443442)
Project Period (FY) 2019-10-07 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2022: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords温度適応 / アノールトカゲ / ニッチシフト / ゲノム
Outline of Research at the Start

アノールトカゲを対象に、温度ニッチシフトの進化機構の解明をめざす。 (1)キューバにおいて開放高温環境に生息するAnolis sagreiが森林内の低温環境に侵入している集団を調査対象として、異なる温度生息地への適応進化に関連している遺伝的変異を検出する[L. Diaz博士との共同]。(2) D. Menke博士との共同で、遺伝子編集による候補遺伝子の機能解析技術を確立する。(3) Tulane大学で、重要な候補遺伝子について遺伝子編集による効果を確認する。 [A. Gunderson博士との共同]。これらの研究を通じて、温度ニッチシフトを可能にした進化機構を解明する。

Outline of Final Research Achievements

The present study aimed to elucidate the genetic basis associated with niche shifts to different temperature environments using Anolis lizards. The genomes of six species inhabiting hot-open and cool-forest habitats were sequenced to detect genes involved in temperature adaptation through genome analysis comparisons. The results of the genome analysis showed that species inhabiting hot-open habitat have higher rates of gene duplication. In addition, conserved and accelerated evolutionary genomic regions were detected in species with hot-open and cool-forest habitats. The results show that these genes predicted to be involved in circadian rhythms, visual development and genes related to lipid metabolism. The gene expression analysis indicated that high TRPV1 expression levels are maintained in lower temperature-preferring species compared to other species.

Academic Significance and Societal Importance of the Research Achievements

温度ニッチシフト機構の解明は、温度環境への適応放散による種多様化機構と温度環境変化への生物の進化的反応の可能性を考える上で大きな意義を持つ。特に、近年温暖化の影響で、外温性脊椎動物である爬虫類の絶滅リスクの増大が危惧されている。本研究の成果は、温度適応に関わる遺伝的基盤を解明することで、どのような遺伝的基盤をもつ種が温度上昇に対して脆弱なのかを特定するための情報を提供する。それにより爬虫類の保全に寄与することが期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (15 results)

All 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] キューバ自然史博物館/ハバナ大学(キューバ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Tulane University(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] キューバ自然史博物館(キューバ)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] University of Georgia/Tulane University(米国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Draft genome of six Cuban Anolis lizards and insights into genetic changes during their diversification2022

    • Author(s)
      Shunsuke Kanamori, Luis M. Diaz, Antonio Cadiz, Katsushi Yamaguchi, Shuji Shigenobu Masakado Kawata
    • Journal Title

      BMC Ecology and Evolution

      Volume: 22 Issue: 1 Pages: 129-129

    • DOI

      10.1186/s12862-022-02086-7

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chronology of embryonic and gonadal development in the Reeves’ turtle, Mauremys reevesii2022

    • Author(s)
      Akashi Hiroshi、Kubota Manami、Yamamoto Hibiki、Miyaoku Kaori、Yamagishi Genki、Miyagawa Shinichi
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-022-15515-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermal Sensitivity of Heat Sensor TRPA1 Correlates With Temperatures Inducing Heat Avoidance Behavior in Terrestrial Ectotherms2021

    • Author(s)
      Akashi Hiroshi
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 9 Pages: 1-9

    • DOI

      10.3389/fevo.2021.583837

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of genes positively selected in Cuban Anolis lizards that naturally inhabit hot and open areas and currently thrive in urban areas2021

    • Author(s)
      Shunsuke Kanamori Antonio Cadiz Luis M. Diaz Yuu Ishii Takuro Nakayama Masakado Kawata
    • Journal Title

      Ecology and Evolution

      Volume: 11 Issue: 4 Pages: 1719-1728

    • DOI

      10.1002/ece3.7161

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] キューバ産およびプエルトリコ産アノールトカゲにおける温度適応に伴う遺伝子発現量進化2022

    • Author(s)
      赤司寛志, 宮川信一, 河田雅圭, Alex Gunderson.
    • Organizer
      日本動物学会第93回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] アノールトカゲの遺伝子発現の温度順化の進化と 転移因子による HSE の拡大との関連2022

    • Author(s)
      金森駿介・河田雅圭
    • Organizer
      第23回日本進化学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] アノールトカゲの温度適応進化に関わるゲノム内保存・加速領域の検出2022

    • Author(s)
      坂本芙久・金森駿介・ Luis M. Diaz・Antonio Cadiz・ 石井悠・ 山口勝司・重信秀治・河田雅圭
    • Organizer
      第23回日本進化学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] etection and comparison of codon bias in eight Anolis species2021

    • Author(s)
      Fuku Sakamoto, Shunsuke Kanamori, Luis M. Diaz, Antonio Cadiz, Yuu Ishii, Takuro Nakayama, Katsushi Yamaguchi, Shuji Shigenobu, and Masakado Kawata
    • Organizer
      The 2nd AsiaEvo Conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] アノールトカゲの温度適応に関わるゲノム内加速領域の検出2021

    • Author(s)
      坂本 芙久・金森 駿介・Luis M. Diaz・Antonio Cadiz・石井 悠・山口 勝司・重信 秀治・河田 雅圭
    • Organizer
      日本生態学会第68回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Chromiumシステムを用いたキューバアノールトカゲ6種の新規ドラフトゲノムの構築と適応進化過程で起きた遺伝的変異の推定2020

    • Author(s)
      金森駿介、Luis M. Diaz、Antonio Cadiz、山口勝司、重信秀治、河田雅圭
    • Organizer
      日本進化学会第22回会
    • Related Report
      2020 Research-status Report
  • [Presentation] 侵略的外来種グリーンアノールとその近縁3種の全ゲノム比較2020

    • Author(s)
      坂本芙久・金森駿介・Luis M Diaz・Antonio Cadiz・石井悠・山口勝司・重信秀治・河田雅圭
    • Organizer
      日本生態学会第67回大会
    • Related Report
      2019 Research-status Report

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Published: 2019-10-10   Modified: 2024-01-30  

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