| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2022: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2021: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2020: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We focused on the cloche zebrafish mutant. We generated npas4l gene-deficient medaka to elucidate the mechanism conserved across species. The npas4l-deficient medaka exhibited a marked decrease in hematopoietic and vascular endothelial cells. Single-cell RNA-seq analysis identified a cell population expressing both hematopoietic and vascular endothelial cell markers, and gene expression analysis suggested that hemangioblasts were localized around the early embryo. In addition, dysplasia of some bones was observed, suggesting the involvement of blood vessels in hard tissue formation. The enhancers that function during fin regeneration in zebrafish were analyzed in medaka, and an enhancer that is strongly expressed during fin regeneration in medaka was identified. It is expected to elucidate the mechanism of hard tissue regeneration that is conserved across species.
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