Strigolactone biosynthesis and its regulation mechanism in plant

• Project/Area Number: 19KK0395
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 38010:Plant nutrition and soil science-related
• Research Institution: Saitama University (2023); Ehime University (2019-2022)
• Principal Investigator: Yoneyama Kaori 埼玉大学, 研究機構, 准教授 (20769997)
• Project Period (FY): 2020 – 2023
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
• Keywords: ストリゴラクトン / シロイヌナズナ / 生合成経路 / ジオキシゲナーゼ / 地上部枝分かれ抑制 / CRISPR/Cas9

Outline of Research at the Start

ストリゴラクトンは、植物の生長・分化を制御する、農業生産においても注目度が高い、極めて重要な二次代謝産物である。本研究では、多様なストリゴラクトンの機能のうち、地上部枝分かれ抑制活性に着目して、シロイヌナズナのストリゴラクトン生合成経路の全貌及び活性本体、更にその調節機構の解明を目指す。そのために、ストリゴラクトン生合成遺伝子LBO (LATERAL BRANCHING OXIDOREDUCTASE）のクレードに近接する4つの遺伝子にコードされているジオキシゲナーゼの酵素機能を明らかにする。

Outline of Final Research Achievements

Strigolactone (SL) is one of important secondary metabolites which regulate plant growth and development. We have previously demonstrated that enzymatic function of Arabidopsis LBO (LATERAL BRANCHING OXIDOREDUCTASE）acting downstream of SL biosynthetic pathway. In the present project, we generated four knockout lines of dioxygenase encoded by genes closed to the LBO clade and examined their shoot branching phenotypes.

Academic Significance and Societal Importance of the Research Achievements

地上部枝分かれ制御は作物の質的良的制限に直結する農業生産上重要な形質に一つであるが、その制御メカニズムはほとんど明らかにされていない。本課題成果は、地上部枝分かれ抑制活性本体の単離構造決定につながるものであり、SL生合成経路の全貌およびその調節メカニズムの解明という学術的意義だけでなく、SL生産制御を介した画期的な農業技術の確立という社会的意義を与えるものである。

Research Products

• [Int'l Joint Research] University of California, Riverside(米国) (2022)
  • Year and Date: 2022-02-15
• [Journal Article] Whispers in the dark: Signals regulating underground plant-plant interactions (2024)
  • Author(s): Yoneyama K, Bennett T
  • Journal Title: Curr Opin Plant Biol Volume: 77 Pages: 1-7
  • DOI: 10.1016/j.pbi.2023.102456
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Stereoselective Strigolactone Biosynthesis Catalyzed by a 2-Oxoglutarate-Dependent Dioxygenase in Sorghum (2023)
  • Author(s): Yoda Akiyoshi、Xie Xiaonan、Yoneyama Kaori、Miura Kenji、McErlean Christopher S P、Nomura Takahito
  • Journal Title: Plant and Cell Physiology Volume: 64 Issue: 9 Pages: 1034-1045
  • DOI: 10.1093/pcp/pcad060
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 植物間コミュニケーションにおけるストリゴラクトンの新しい機能 (2023)
  • Author(s): 米山香織
  • Journal Title: 植物の生長調節 Volume: 58 Pages: 100-104
• [Journal Article] Ancestral sequence reconstruction of the CYP711 family reveals functional diverfence in strigolactone biosynthetic enzymes associated with gene duplication events in monocot grasses (2022)
  • Author(s): Vinde MH, Cao D, Chesterfield RJ, Yoneyama K*, Gumulya Y, Thomson RES, Matila T, Ebert BE, Beveridge CA, Vickers CE, Gillam EMJ
  • Journal Title: New Phytol Volume: 235 Issue: 5 Pages: 1900-1912
  • DOI: 10.1111/nph.18285
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An ancestral function of strigolactones as symbiotic rhizosphere signals. (2022)
  • Author(s): Kodama K, Rich k, Yoda A, Shimazaki S, Xie X, Akiyama K.,Mizuno Y, Komatsu A., Luo Y, Suzuki H, Kameoka H, Libourel C, Keller J, Sakakibara K, Nishiyama T, Nakagawa T, Mashiguchi K, Uchida K, Yoneyama K, Tanaka Y, Yamaguchi S, Shimamura M , Delaux P, Nomu
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 3974-3974
  • DOI: 10.1038/s41467-022-31708-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Environmental strigolactone drives early growth responses to neighbouring plants and soil volume in pea (2022)
  • Author(s): Wheeldon CD, Hamon-Josse M, Lund H, Yoneyama K*, Bennett T
  • Journal Title: Curr. Biol Volume: 32 Issue: 16 Pages: 3593-3600
  • DOI: 10.1016/j.cub.2022.06.063
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Supra-organismal regulation of strigolactone exudation and plant development in rice in response to rhizospheric cues (2022)
  • Author(s): Yoneyama K*, Xie X, Nomura T, Yoneyama K, Bennett T
  • Journal Title: Curr. Biol Volume: 32 Issue: 16 Pages: 3601-3608
  • DOI: 10.1016/j.cub.2022.06.047
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extraction and Quantification of Plant Hormones and RNA from Pea Axillary Buds (2022)
  • Author(s): Cao D, Barbier F, Yoneyama K *, Beveridge CA
  • Journal Title: Bio-protocol Volume: 12 Pages: 1-8
  • DOI: 10.3389/fpls.2020.605069
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Germination stimulant activity of isothiocyanates on Phelipanche spp (2022)
  • Author(s): Hinako Miura 、Ryota Ochi、Hisashi Nishiwaki、Satoshi Yamauchi、Xiaonan Xie、Hidemitsu Nakamura、Koichi Yoneyama、Kaori Yoneyama
  • Journal Title: Plants Volume: 24 Issue: 5 Pages: 606-606
  • DOI: 10.3390/plants11050606
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ストリゴラクトン生合成経路と地上部枝分かれ抑制活性本体の解明—地上部枝分かれ抑制活性本体を追え— (2021)
  • Author(s): 米山 香織
  • Journal Title: Japanese Journal of Pesticide Science Volume: 46 Issue: 2 Pages: 177-178
  • DOI: 10.1584/jpestics.W21-16
  • NAID: 130008162143
  • ISSN: 2187-0365, 2187-8692
  • Year and Date: 2021-08-20
• [Journal Article] Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum (2021)
  • Author(s): Yoda Akiyoshi、Mori Narumi、Akiyama Kohki、Kikuchi Mayu、Xie Xiaonan、Miura Kenji、Yoneyama Kaori、Sato‐Izawa Kanna、Yamaguchi Shinjiro、Yoneyama Koichi、Nelson David C.、Nomura Takahito
  • Journal Title: New Phytologist Volume: 232 Issue: 5 Pages: 1999-2010
  • DOI: 10.1111/nph.17737
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Strigolactones, how are they synthesized to regulate plant growth and development? (2021)
  • Author(s): Yoneyama Kaori、Brewer Philip B
  • Journal Title: Current Opinion in Plant Biology Volume: 63 Pages: 102072-102072
  • DOI: 10.1016/j.pbi.2021.102072
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure?activity relationship of the aromatic moiety of 6-substituted 5,6-dihydro-2<i>H</i>-pyran-2-one to find the novel compound showing higher plant growth inhibitory activity (2021)
  • Author(s): Ochi Ryota、Yoneyama Kaori、Nishiwaki Hisashi、Yamauchi Satoshi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 86 Issue: 2 Pages: 165-169
  • DOI: 10.1093/bbb/zbab185
  • Peer Reviewed
• [Journal Article] Do phosphate and cytokinin interact to regulate strigolactone biosynthesis or act independently? (2020)
  • Author(s): Kaori Yoneyama et al
  • Journal Title: frontiers in Plant Science Volume: in press Pages: 1-9
  • DOI: 10.3389/fpls.2020.00438
  • Peer Reviewed / Open Access
• [Journal Article] Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis (2020)
  • Author(s): Yoneyama Kaori、Akiyama Kohki、Brewer Philip B.、Mori Narumi、Kawano‐Kawada Miyuki、Haruta Shinsuke、Nishiwaki Hisashi、Yamauchi Satoshi、Xie Xiaonan、Umehara Mikihisa、Beveridge Christine A.、Yoneyama Koichi、Nomura Takahito
  • Journal Title: Plant Direct Volume: 4 Issue: 5 Pages: 1-14
  • DOI: 10.1002/pld3.219
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 植物-植物コミュニケーションを利用した作物の耐性強化を目指して (2023)
  • Author(s): 米山香織
  • Organizer: 令和5年度日本学術会議公開シンポジウム
  • Invited
• [Presentation] ストリゴラクトン生合成に関与するオオムギLBOの機能解析 (2023)
  • Author(s): 井上舞子、Philip Brewer、謝肖男、黒岩風、戸澤譲、米山香織
  • Organizer: 植物化学調節学会第58回大会
• [Presentation] 養分条件がシロイヌナズナのストリゴラクトン生合成に与える影響 (2023)
  • Author(s): 崎岡莉子、黒瀬凌、米山香織
  • Organizer: 植物化学調節学会第58回大会
• [Presentation] ストリゴラクトンを介したポット容量認識 (2023)
  • Author(s): 下田萌乃、Tom Bennett、米山香織
  • Organizer: 植物化学調節学会第58回大会
• [Presentation] ストリゴラクトンを介した植物間の根圏コミュニケーション (2023)
  • Author(s): 米山香織
  • Organizer: 日本植物学会第87回大会
  • Invited
• [Presentation] Regulation of strigolactone biosynthesis/exudation (2023)
  • Author(s): Kaori Yoneyama
  • Organizer: The 24th International Conference on Plant Growth Substances
  • Int'l Joint Research / Invited
• [Presentation] ストリゴラクトン生産・分泌の環境応答 (2021)
  • Author(s): 米山香織
  • Organizer: 日本植物生理学会
  • Invited
• [Presentation] 根圏に分泌されるストリゴラクトンの新たな機能とその分泌制御メカニズム (2021)
  • Author(s): 米山香織
  • Organizer: 日本植物学会第85回大会
  • Invited
• [Presentation] The Possibility of Strigolactones for Plant-Plant Communication (2021)
  • Author(s): Kaori Yoneyama
  • Organizer: 3rd International Congress on Strigolactones
  • Int'l Joint Research / Invited
• [Presentation] Strigolactones, how are they synthesized to regulate plant growth and development? (2021)
  • Author(s): Kaori Yoneyama
  • Organizer: IRN France-Japan Frontiers in Plant Biology on “Photosynthesis and Metabolism”
  • Int'l Joint Research / Invited
• [Presentation] イソチオシアネートによる根寄生雑草の発芽刺激活性 (2021)
  • Author(s): 三浦妃奈子、米山香織
  • Organizer: 日本農芸化学会西日本・中四国・関西支部 2021年度合同大会