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Analysis of the extracellular vesicle secretion mechanism of a plant pathogenic fungus using chemical biology

Research Project

Project/Area Number 19KK0397
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))

Allocation TypeMulti-year Fund
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kumakura Naoyoshi  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (50815438)

Project Period (FY) 2021 – 2023
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords炭疽病菌 / ゲノム編集 / 付着器 / マーカーリサイクリング / 病原性因子 / CRISPR-Cas9 / アブラナ科炭疽病菌 / エフェクター / EV / 植物病原糸状菌 / 分泌 / 阻害剤 / Extracellular vesicle
Outline of Research at the Start

植物病原糸状菌はエフェクターと呼ばれる小分子分泌性タンパク 質を駆使することで感染を成立させる。近年、これらエフェクターがExtracellular vesicle(EV)を介して 分泌される可能性が示唆された。EVは細胞が分泌する膜小胞であり、シグナル分子 を運搬することで細胞間コミュニケーションを担うが、EV分泌に寄与するメカニズムは不明な点が多い。申請者はこれまでに、エフェクター分泌を阻害する複数の化合物を同定している。この化合物の中から、EV分泌を阻害するものを探索し、ケミカルバイオロジー的手法を用いて、植物病原糸状菌のEV分泌メカニズムを解明する。

Outline of Final Research Achievements

Through collaborative research with Dr. Richard O'Connell, three significant outcomes were achieved. Firstly, it was suggested that the target of compounds identified by the applicant in their basic project, which possess effector secretion inhibition effects, may not be the EV secretion mechanism. Secondly, new technology was established in the Brassicaceae anthracnose fungus, a plant pathogenic filamentous fungus, leading to publication in an international journal as a co-authored paper. Thirdly, using the aforementioned technology, it became possible to elucidate the functions of key factors contributing to the pathogenicity of the anthracnose fungus. These results are scheduled for submission as an international co-authored paper by 2024, with some of the findings planned for patent application. Thus, this support has borne fruit in the form of multiple original papers, patent applications, and ongoing collaborative research efforts.

Academic Significance and Societal Importance of the Research Achievements

植物病原糸状菌は、多大な作物被害を引き起こすため、その病原性メカニズムを理解することは食糧の安全保障上、重要である。この研究では、植物病原糸状菌の病原性メカニズムを解明するためのゲノム編集技術と解析技術の開発が実現した。これらの成果は、炭疽病菌の病原性メカニズムの理解に貢献し、実際に申請者の三報の筆頭著者論文として結実し、2024年度中にさらに一報を報告する予定である。これらの結果は、病原性メカニズムの理解とそれを利用した植物病原糸状菌による作物被害の技術開発への寄与が期待できる

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (22 results)

All 2024 2023 2022 2021

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 8 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results)

  • [Int'l Joint Research] INRAE(フランス)2021

    • Year and Date
      2021-06-15
    • Related Report
      2023 Annual Research Report
  • [Journal Article] Guanosine‐specific single‐stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses2024

    • Author(s)
      Kumakura Naoyoshi、Singkaravanit‐Ogawa Suthitar、Gan Pamela、Tsushima Ayako、Ishihama Nobuaki、Watanabe Shunsuke、Seo Mitsunori、Iwasaki Shintaro、Narusaka Mari、Narusaka Yoshihiro、Takano Yoshitaka、Shirasu Ken
    • Journal Title

      New Phytologist

      Volume: 242 Issue: 1 Pages: 170-191

    • DOI

      10.1111/nph.19582

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3‐based marker recycling2023

    • Author(s)
      Yonehara Katsuma、Kumakura Naoyoshi、Motoyama Takayuki、Ishihama Nobuaki、Dallery Jean‐F?lix、O'Connell Richard、Shirasu Ken
    • Journal Title

      Molecular Plant Pathology

      Volume: 24 Issue: 11 Pages: 1451-1464

    • DOI

      10.1111/mpp.13378

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient multiple gene knock-out in Colletotrichum higginsianum via CRISPR-Cas9 ribonucleoprotein and URA3-based marker recycling2023

    • Author(s)
      Yonehara Katsuma、Kumakura Naoyoshi、Motoyama Takayuki、Ishihama Nobuaki、Dallery Jean-Felix、O’Connell Richard、Shirasu Ken
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2023.04.20.537420

    • Related Report
      2022 Research-status Report
    • Open Access / Int'l Joint Research
  • [Journal Article] A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants2023

    • Author(s)
      Chen Mingming、Kumakura Naoyoshi、Saito Hironori、Muller Ryan、Nishimoto Madoka、Mito Mari、Gan Pamela、Ingolia Nicholas T、Shirasu Ken、Ito Takuhiro、Shichino Yuichi、Iwasaki Shintaro
    • Journal Title

      eLife

      Volume: 12

    • DOI

      10.7554/elife.81302

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Conserved Colletotrichum spp. Effector Candidate CEC3 Induces Nuclear Expansion and Cell Death in Plants2021

    • Author(s)
      Tsushima Ayako、Narusaka Mari、Gan Pamela、Kumakura Naoyoshi、Hiroyama Ryoko、Kato Naoki、Takahashi Shunji、Takano Yoshitaka、Narusaka Yoshihiro、Shirasu Ken
    • Journal Title

      Frontiers in Microbiology

      Volume: 12

    • DOI

      10.3389/fmicb.2021.682155

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses2021

    • Author(s)
      Kumakura Naoyoshi、Singkaravanit-Ogawa Suthitar、Gan Pamela、Tsushima Ayako、Ishihama Nobuaki、Watanabe Shunsuke、Seo Mitsunori、Iwasaki Shintaro、Narusaka Mari、Narusaka Yoshihiro、Takano Yoshitaka、Shirasu Ken
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2021.10.13.464185

    • Related Report
      2021 Research-status Report
    • Open Access
  • [Journal Article] Telomeres and a repeat‐rich chromosome encode effector gene clusters in plant pathogenicColletotrichumfungi2021

    • Author(s)
      Gan Pamela、Hiroyama Ryoko、Tsushima Ayako、Masuda Sachiko、Shibata Arisa、Ueno Akiko、Kumakura Naoyoshi、Narusaka Mari、Hoat Trinh Xuan、Narusaka Yoshihiro、Takano Yoshitaka、Shirasu Ken
    • Journal Title

      Environmental Microbiology

      Volume: - Issue: 10 Pages: 6004-6018

    • DOI

      10.1111/1462-2920.15490

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative transient expression analyses on two conserved effectors of Colletotrichum orbiculare reveal their distinct cell death‐inducing activities between Nicotiana benthamiana and melon2021

    • Author(s)
      Chen Jinlian、Inoue Yoshihiro、Kumakura Naoyoshi、Mise Kazuyuki、Shirasu Ken、Takano Yoshitaka
    • Journal Title

      Molecular Plant Pathology

      Volume: - Issue: 8 Pages: 1006-1013

    • DOI

      10.1111/mpp.13078

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Toward identification of host cell death inducing genes in an NLR dependent manner in Colletotrichum higginsianum2024

    • Author(s)
      Yonehara Katsuma, Naoyoshi Kumakura, Shirasu Ken
    • Organizer
      32nd Fungal Genetics Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ウリ類炭疽病菌の分泌性リボヌクレアーゼは宿主植物の免疫反応を増強する2024

    • Author(s)
      Naoyoshi Kumakura, Suthitar Singkaravanit-Ogawa, Pamela Gan, Ayako Tsushima, Nobuaki Ishihama, Shunsuke Watanabe, Mitsunori Seo, Shintaro Iwasaki, Mari Narusaka, Yoshihiro Narusaka, Yoshitaka Takano, Ken Shirasu
    • Organizer
      令和六年度日本植物病理学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Toward identification of host cell death-inducing genes of Colletotrichum higginsianum via transient gene expression in Arabidopsis thaliana leaves2023

    • Author(s)
      Yonehara Katsuma, Naoyoshi Kumakura, Shirasu Ken
    • Organizer
      The 33rd International Conference on Arabidopsis Research (ICAR2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アブラナ科炭疽病菌におけるCRISPR-Cas9システムを用いたマーカーリサイクリング法による多重遺伝子破壊法の開発2023

    • Author(s)
      Yonehara, Katsuma; Kumakura, Naoyoshi;Motoyama, Takayuki;Dallery, Jean-Felix;O'Connell, Richard;Shirasu, Ken
    • Organizer
      令和5年度日本植物病理学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Towards identification of virulence factors contributing to the necrotrophic phase of Colletotrichum orbiculare2023

    • Author(s)
      Yonehara, Katsuma;Kumakura, Naoyoshi;Gan, Pamela Hui Peng;Shirasu, Ken
    • Organizer
      16th European Conference on Fungal Genetics
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses2023

    • Author(s)
      Kumakura, Naoyoshi;Singkaravanit-Ogawa, Suthitar;Gan, Pamela;Tsushima, Ayako;Ishihama, Nobuaki;Watanabe, Shunsuke;Seo, Mitsunori;Iwasaki, Shintaro;Narusaka, Mari;Narusaka, Yoshihiro;Takano, Yoshitaka;Shirasu, Ken
    • Organizer
      16th European Conference on Fungal Genetics
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 共働き夫婦の海外留学・家事育児分担の一事例2023

    • Author(s)
      熊倉直祐
    • Organizer
      日本農芸化学会 2023年度大会 シンポジウム ライフイベントとキャリアパス 結婚・出産・育児は選択の連続、その中でどのような選択をしてきたかのリアルな体験談
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 植物病原糸状菌研究の壁 ~炭疽病菌の事例~2022

    • Author(s)
      熊倉直祐
    • Organizer
      糸状菌分子生物学研究会若手の会 第10回ワークショップ
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 植物二次代謝物を介した植物-糸状菌間の軍拡競争2022

    • Author(s)
      熊倉直祐
    • Organizer
      第21回糸状菌分子生物学コンファレンス
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Identification of secondary metabolite synthesis genes involved in virulence of Colletotrichum fungi using a multiplex gene disruption system2021

    • Author(s)
      Naoyoshi Kumakura
    • Organizer
      Seminar at BIOGER in INRAE
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Guanosine-specific single-stranded ribonuclease effectors of a phytopathogenic fungus potentiate host immune responses2021

    • Author(s)
      Naoyoshi Kumakura, Suthitar Singkaravanit-Ogawa, Pamela Gan,Ayako Tsushima, Nobuaki Ishihama, Shunsuke Watanabe, Mitsunori Seo, Shintaro Iwasaki, Mari Narusaka, Yoshihiro Narusaka, Yoshitaka Takano, Ken Shirasu
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Rocaglateを生合成するアグライア属植物に寄生する糸状菌は変異型eIF4Aを利用して生存する2021

    • Author(s)
      熊倉直祐、Mingming Chen、Ryan Muller、七野悠一、西本まどか、水戸麻理、Pamela Gan、Nicholas T. Ingolia、白須賢、伊藤拓宏、岩崎信太郎
    • Organizer
      熊倉直祐、Mingming Chen、Ryan Muller、七野悠一、西本まどか、水戸麻理、Pamela Gan、Nicholas T. Ingolia、白須賢、伊藤拓宏、岩崎信太郎
    • Related Report
      2021 Research-status Report
  • [Presentation] ウリ類炭疽病菌感染における殺生段階成立に必要な病原性因子の探索2021

    • Author(s)
      米原克磨、熊倉直祐、Pamela Gan、白須賢
    • Organizer
      令和4年度日本植物病理学会大会
    • Related Report
      2021 Research-status Report

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Published: 2020-02-06   Modified: 2025-01-30  

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