Regulation of transformation and cell growth by molecules controlling cell death

• Project/Area Number: 20013020
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: YONEHARA Shin Kyoto University, 大学院・生命科学研究科, 教授 (00124503)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥16,000,000 (Direct Cost: ¥16,000,000); Fiscal Year 2009: ¥8,000,000 (Direct Cost: ¥8,000,000); Fiscal Year 2008: ¥8,000,000 (Direct Cost: ¥8,000,000)
• Keywords: 細胞死 / 遺伝子 / 癌 / 細胞・組織 / シグナル伝達

Research Abstract

We show that tetraploid cells induced by impaired mitotic chromosomal condensation are eliminated by a novel type of cell death different from caspase-dependent apoptosis. The cell death was executed by the downregulation of eukaryotic translation elongation factor 1 alpha 1 (eEF1A1), a typical housekeeping gene products. The downregulation of eEF1A1 expression was shown to be induced by both destabilization of its mRNA and its translational inhibition.

Research Products

• [Journal Article] Novel cell death by downregulation of eEF1A1 expression in tetraploids. (2009)
  • Author(s): Kobayashi Y, Yonehara S
  • Journal Title: Cell Death Differ 16 Pages: 139-150
• [Journal Article] Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase. (2009)
  • Author(s): Kiriyama M, Kobayashi Y, Saito M, Ishikawa F, Yonehara S
  • Journal Title: Mol Cell Biol 29 Pages: 4742-4756
• [Journal Article] RPA-like Mammalian Ctc1-Stn1-Ten1 Complex Binds to Single-Stranded DNA and Protects Telomeres Independently of the Pot1 Pathway. (2009)
  • Author(s): Miyake Y, Nakamura M, Nabetani A, Shimamura S, Tamura M, Yonehara S, Saito M, Isikawa F
  • Journal Title: Mol Cell 36 Pages: 193-206
• [Journal Article] Novel cell death by downregulation of eEF1A1 expression in tetraploids. (2009)
  • Author(s): Kobayashi Y, Yonehara S
  • Journal Title: Cell Death and Differentiation 16 Pages: 139-150
  • Peer Reviewed
• [Journal Article] RPA-like Mammalian Ctcl-Stnl-Tenl Complex Binds to Single-Stranded DNA and Protects Telomeres Independently of the Potl Pathway (2009)
  • Author(s): Miyake Y, Nakamura M, Nabetani A, Shimamura S, Tamura M, Yonehara S, Saito M, Isikawa F
  • Journal Title: Mol Cell 36 Pages: 193-206
  • Peer Reviewed
• [Journal Article] Novel cell death by downregulation of eEF1A1 expression in tetraploids (2009)
  • Author(s): Kobayashi Y, and Yonehara S
  • Journal Title: Cell Death and Differentiation 16 Pages: 139-150
  • Peer Reviewed
• [Journal Article] Mechanism of NKT cell activation by intranasal coadministration of α-galactosylceramide, which can induce cross-protection against influenza viruses (2008)
  • Author(s): Kamijuku H, Nagata Y, Jiang X, Ichinohe T, Tashiro T, Mori K, Taniguchi M, Hase K, Ohno H, Shimaoka T, Yonehara S, Odagiri T, Tashiro M, Sata T, Hasegawa H, Seino K
  • Journal Title: Mucosal Immunology 1 Pages: 208-218
  • Peer Reviewed
• [Presentation] Novel cell death by downregulation of eEFlA1 expression in tetraploids (2009)
  • Author(s): Shin Yonehara
  • Organizer: The 5th NTU-Kyoto U Mini-Symposium on Molecular and Cell Biology
  • Place of Presentation: Kyoto
  • Year and Date: 2009-05-23
• [Presentation] Novel cell death by downregulation of eEF1A1 expression in tetraploids (2009)
  • Author(s): Shin Yonehara
  • Organizer: The 5th NTU-Kyoto U Mini-Symposium on Molecular and Cell Biology
  • Place of Presentation: Kyoto
  • Year and Date: 2009-03-23
• [Presentation] Death Receptor Fasと自己免疫疾患・炎症 (2008)
  • Author(s): 米原伸
  • Organizer: 第29回日本炎症・再生医学会シンポジウム「アポトーシスは炎症の終息と再生の始まりである」
  • Place of Presentation: 東京都
  • Year and Date: 2008-07-09
• [Presentation] Physiological roles of Fas (2008)
  • Author(s): Shin Yonehara
  • Organizer: Independent Symposium: Future Prospective New Drug, MEDICAL-EXPO 2008 in APLAR's World: The 13th Congress of the Asia Pacific League of Associations for Rheumatology
  • Place of Presentation: Yokohama
• [Presentation] Novel types of cell death identified in 1989 and 2008 (2008)
  • Author(s): Shin Yonehara
  • Organizer: Argenes Sponsored Symposium:Apoptotic Therapy in RA, MEDICAL-EXPO 2008 in APLAR's World: The 13th Congress of the Asia Pacific League of Associations for Rheumatology
  • Place of Presentation: Yokohama
• [Presentation] Novel caspase-independent cell death by downregulation of eEF1A1/EF-1αexpression in tetraploids with chromosomal aberrations (2008)
  • Author(s): Shin Yonehara
  • Organizer: The 15th East Asia Joint Conference on Biomedical Research
  • Place of Presentation: Seoul, Korea
• [Patent(Industrial Property Rights)] 細胞増殖抑制剤及びそのスクリーニング法 (2009)
  • Inventor(s): 京都大学大学院生命科学研究科教授米原伸, 他3名
  • Industrial Property Rights Holder: 京都大学大学院生命科学研究科教授米原伸, 他3名
  • Industrial Property Number: 2009-052628
  • Filing Date: 2009-03-05