Studies on the genomic basis for establishment of multicellular systems using the social amoebae

• Project/Area Number: 20017004
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: URUSHIHARA Hideko University of Tsukuba, 大学院・生命環境科学研究科, 教授 (00150087)
• Co-Investigator(Kenkyū-buntansha): KUWAYAMA Hidekazu 筑波大学, 大学院・生命環境科学研究科, 講師 (40397659)
• Co-Investigator(Renkei-kenkyūsha): KAWATA Takefumi 東邦大学, 理学部, 准教授 (30221899)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥16,000,000 (Direct Cost: ¥16,000,000); Fiscal Year 2009: ¥8,000,000 (Direct Cost: ¥8,000,000); Fiscal Year 2008: ¥8,000,000 (Direct Cost: ¥8,000,000)
• Keywords: 微生物ゲノム / ゲノム進化 / 比較ゲノム / 発生分化 / 細胞性粘菌 / 多細胞体制 / 細胞分化 / ゲノム解析 / データベース / cDNA解析 / 進化 / 遺伝子モデル

Research Abstract

The genome and cDNA analyses were performed in Acytostelium subglobosum, a cellular slime mold species without stalk-cell differentiation, and approximately 13,000 genes were identified. They included orthologues for most of the cell-differentiation related genes in Dictyostelium discoideum, the most widely used species with cell-differentiation abilities. However, closer examination of multi-gene families revealed that the family compositions were dissimilar between the two species.

Research Products

• [Journal Article] The cellular slime mold: eukaryotic model microorganism. (2009)
  • Author(s): Urushihara, H.
  • Journal Title: Exp. Anim. 58 Pages: 977-104
  • NAID: 10024838149
  • Peer Reviewed
• [Journal Article] Switching direction in electric signal-induced cell migration by cGMP and phosphatydylinositol signaling. (2009)
  • Author(s): Sato, MJ. Kuwayama, H., van Egmond, WN., Takayama, ALK, Takagi, H., van Haastert, PJM., Yanagida, T., Ueda, M.
  • Journal Title: Proc. Natl. Acad. Sci. 106 Pages: 6667-6672
  • Peer Reviewed
• [Journal Article] Differentiation-Inducing Factor-1 and -2 function also as Modulators for Dictyostelium Chemotaxis. (2009)
  • Author(s): Kuwayama, H., Kubohara, Y.
  • Journal Title: PLoS One 4
  • NAID: 120007131231
  • Peer Reviewed
• [Journal Article] The cellular slime mold : eukaryotic model microorganism (2009)
  • Author(s): Urushihara, H.
  • Journal Title: Exp.Anim. 58 Pages: 977-104
  • NAID: 10024838149
  • Peer Reviewed
• [Journal Article] Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis (2009)
  • Author(s): Kuwayama, H.
  • Journal Title: PLoS One 4
  • NAID: 120007131231
  • Peer Reviewed
• [Journal Article] Developmental biology of the social amoeba: history, current knowledge and prospects. (2008)
  • Author(s): Urushihara, H.
  • Journal Title: Dev Growth Differ. 50Suppl1
  • NAID: 10024400567
  • Peer Reviewed
• [Journal Article] Evolutionary linkage between cytokinesis and chloroplast division by dynamin proteins. (2008)
  • Author(s): Miyagishima, H., Kuwayama, H., Urushihara, H., Nakanishi, H.
  • Journal Title: Proc. Natl. Acad. Sci. 105 Pages: 15202-15207
  • Peer Reviewed
• [Journal Article] DNA oligonucleotide-assisted genetic manipulation increases transformation and homologous recombination efficiencies ; evidence from gene targeting of Dictyoste-lium discoideum (2008)
  • Author(s): Kuwayama, H
  • Journal Title: J. Bacteriol 133 Pages: 418-423
  • Peer Reviewed
• [Journal Article] Regulation of ammonia homeostasis by the ammonium transporter AmtA in Dictyostelium discoideum (2008)
  • Author(s): Yoshino, R
  • Journal Title: Eukaryot Cell 6 Pages: 2419-2428
  • Peer Reviewed
• [Journal Article] Developmental biology of the social amoeba : History, current know-ledge and prospects (2008)
  • Author(s): Urushihara, H
  • Journal Title: Develop. Growth Differ 50
  • NAID: 10024400567
  • Peer Reviewed
• [Journal Article] Evolutionary linkage between cytokinesis and chloroplast division by dynamin proteins (2008)
  • Author(s): Miyagishima, H
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 15202-15207
  • Peer Reviewed
• [Presentation] 非分化性の社会性アメーバ、Acytosteliumの遺伝子解析 (2010)
  • Author(s): 漆原秀子、桑山秀一、鹿児島浩、新井理、大石加寿子、伊藤武彦、谷口丈晃、豊田敦、野口英樹、内今日子、黒木陽子、畑敬士、磯辺拓海、小原雄治、藤山秋佐夫
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-03-09
• [Presentation] 細胞性粘菌Dictyostelium discoideumにおいて生活環周期を調節する新規rabGAPの機能解析 (2010)
  • Author(s): 桑山秀一、上田昌宏、漆原秀子
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-03-09
• [Presentation] 非分化性の社会性アメーバ、Acytostelium の遺伝子解析 (2010)
  • Author(s): 漆原秀子
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-03-09
• [Presentation] 細胞性粘菌 Dictyostelium discoideum において生活環周期を調節する新規rabGAPの機能解析 (2010)
  • Author(s): 桑山秀一
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-03-09
• [Presentation] Gene repertoir of Acytostelium subglobosum, a group2 species without stalk-cell differentiation (2009)
  • Author(s): 漆原秀子
  • Organizer: International Dictyostelium Conference 2009
  • Place of Presentation: Denver, USA
  • Year and Date: 2009-08-25
• [Presentation] Gene repertoire of Acytostelium subglobosum, a group 2 species without stalk-cell differentiation (2009)
  • Author(s): Urushihara, H., Kuwayama, H., Kagoshima, H., Shin-I, T., Itoh, T., Taniguchi, T., Uchi, K., Kuroki, Y., Kohara, Y., Fujiyama, A.
  • Organizer: International Dictyostelium Conference 2009
  • Place of Presentation: Denver, USA
  • Year and Date: 2009-08-24
• [Presentation] Cell-differentiation related genes in a non-differentiating cellular slime mould revealed by its genome analysis (2009)
  • Author(s): Kuwayama, H., Uchi K., Kagoshima, H., Shin-I, T., Ohishi, K., Itoh, T, Taniguchi, T., Kuroki, Y., Kohara, Y., Fujiyama, A., Urushihara, H.
  • Organizer: 第42回日本発生生物学会年会
  • Place of Presentation: 新潟トキメッセ
  • Year and Date: 2009-05-30
• [Presentation] Analysis of a cell-differentiation related gene in a non-differentiating cellular slime mould (2009)
  • Author(s): Tohyama T., Kuwayama, H., Urushihara, H.
  • Organizer: 第42回日本発生生物学会年会
  • Place of Presentation: 新潟トキメッセ
  • Year and Date: 2009-05-29
• [Presentation] Analysis of a cell-differentiation related gene in a non-differentiating cellular slime mould (2009)
  • Author(s): Tohyama, T.
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟県トキメッセ
  • Year and Date: 2009-05-29
• [Presentation] Cell-differentiation related genes in a non-differentiating cellular slime mould revealed by its genome analysis (2009)
  • Author(s): 桑山秀一
  • Organizer: 第42回日本発生生物学会大会
  • Place of Presentation: 新潟県トキメッセ
  • Year and Date: 2009-05-29
• [Presentation] Acytostelium属細胞性粘菌のゲノム解析 (2009)
  • Author(s): 漆原秀子、桑山秀一、鹿児島浩、新井理、大石加寿子、伊藤武彦、谷口丈晃、内今日子、黒木陽子、小原雄治、藤山秋佐夫
  • Organizer: 第3回日本ゲノム微生物学会年会
  • Place of Presentation: 中央大学後楽園キャンパス
  • Year and Date: 2009-03-05
• [Presentation] Acytostelium属細胞性粘菌のゲノム・cDNA解析 (2009)
  • Author(s): 漆原 秀子
  • Organizer: 第3回日本ゲノム微生物学会年会
  • Place of Presentation: 中央大学
  • Year and Date: 2009-03-05
• [Presentation] Two resource sites from Japan. (2008)
  • Author(s): Urushihara, H.
  • Organizer: InternationalDictyostelium Conference 2008 (Workshop)
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-20
• [Presentation] Acytostelium subglobosum genome analysis (2008)
  • Author(s): Urushihara, H
  • Organizer: International Dictyostelium Conference 2008
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-20
• [Presentation] Developmental genes in Acytostelium subglobosum, a group 2 species without cell- type differentiation (2008)
  • Author(s): Urushihara, H., Kuwayama, H., Uchi, K., Kohara, Y., Kagoshima, H., Shin-I, T., Ohishi, K., Itoh, T., Taniguchi, T., Fujiyama, A.
  • Organizer: International Dictyostelium Conference 2008
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-18
• [Presentation] Developmental inhibitor secreted by Acytostelium subglobosum, a primitive social amoeba without cell differentiation (2008)
  • Author(s): Uchi, K., Kuwayama, H., Yoshino, R., Urushihara, H.
  • Organizer: International Dictyostelium Conference 2008
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-18
• [Presentation] Functional analysis of rasX group genes (2008)
  • Author(s): Furuya, Y., Kunitani, M., Muramoto, T., Kuwayama, H., Urushihara, H.
  • Organizer: International Dictyostelium Conference 2008
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-18
• [Presentation] Differential gene expression between sexually mature and immature cells of Dictyostelium discoideum (2008)
  • Author(s): Saeki, K., Satoh, T., Muramoto, T., Skeleton, J., Kay, RR., Kuwayama, H., Urushihara, H.
  • Organizer: International Dictyostelium Conference 2008
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-18
• [Presentation] Developmental inhibitor of Acytostelium subglobosum that forms fruiting bodies without cell differentiation (2008)
  • Author(s): Uchi, K
  • Organizer: International Dictyostelium Conference 2008
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-16
• [Presentation] Comparative genomic analysis of the cellular slime moulds to reveal how cell differentiation was established during evolution (2008)
  • Author(s): Kuwayama, H
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-28
• [Presentation] Genome-wide search for cell-fusion related genes based on DNA mcroarray analysis in Dictyostelium (2008)
  • Author(s): Saeki, K
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-27
• [Presentation] Functional analysis of newly identified Dictyostelium Ras genes (2008)
  • Author(s): Kunitani, M
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-27
• [Presentation] Developmental inhibitor secreted by Acytostelium subglobosum, aprimitive social amoeba without cell differentiation (2008)
  • Author(s): Uchi, K
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2008-05-27
• [Book] 「dictyBase-社会性アメーバ『細胞性粘菌』の総合データベース-」細胞工学別冊「バイオリソース&データベース活用術」 (2009)
  • Author(s): 漆原秀子
  • Publisher: 秀潤社
• [Book] 「研究をささえるモデル生物-実験室いきものガイド」第10章「単細胞と多細胞の架け橋-細胞性粘菌がわかる10. 1概論」 (2009)
  • Author(s): 漆原秀子
  • Publisher: 化学同人
• [Book] 「研究をささえるモデル生物-実験室いきものガイド」第10章「単細胞と多細胞の架け橋-細胞性粘菌がわかる」10. 2最新研究「細胞性粘菌の多細胞化のしくみにゲノム科学で迫る」 (2009)
  • Author(s): 漆原秀子
  • Publisher: 化学同人
• [Book] 研究をささえるモデル生物(第10章) (2009)
  • Author(s): 漆原秀子
  • Total Pages: 262
  • Publisher: 化学同人
• [Remarks] Acytostelium gene database (AcytoDB)・公開版
  • URL: http://acyto.sequence.info/
• [Remarks] ・開発版:(近日公開)
  • URL: http://gbas201002.www2.sequence.info/
• [Remarks] 研究室ホームページ
  • URL: http://nenkin.gene.tsukuba.ac.jp/
• [Remarks] Acytostelium gene database
  • URL: http://acyto.sequence.info/
• [Remarks]
  • URL: http://acyto.sequence.info/