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血流循環動態に基づく血管内皮前駆細胞機能評価法の確立

Research Project

Project/Area Number 20034054
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionAichi Gakuin University

Principal Investigator

成瀬 桂子  Aichi Gakuin University, 歯学部, 准教授 (30387576)

Co-Investigator(Kenkyū-buntansha) 松原 達昭  , 歯学部, 教授 (30209598)
小林 泰子  , 歯学部, 講師 (40418926)
中村 二郎  名古屋大学, 医学系研究科, 准教授 (40283444)
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2009: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2008: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords血管内皮前駆細胞 / 細胞運動 / 管腔形成 / 細胞機能
Research Abstract

血管内皮前駆細胞(EPCs)による血管修復作用のin vitroにおける再現を目指し,wound scrapeモデルを用いて,EPCsの細胞運動をタイムラプス付蛍光顕微鏡下で全過程を可視化した.その結果,EPCsはwoundに対し,ラメリポディアを形成しながら遊走していくものの,一定の場所においてwoundに平行にラインを形成することが明らかとなった.今のところ,何がこのラインの位置を規定しているかは明らかでないが,こうしたライン形成能が血管発生、血管修復に大きく寄与している可能性が考えられた.EPCsと成熟血管内皮細胞(ECs)をマトリジェル上でそれぞれ培養すると,EPCs単独で管腔形成能はほとんどなく,一方で細胞運動能は非常に活発でマトリジェル上で動き続けているが,ECsはすぐに全体に管腔を形成する.EPCsとECsの共培養系では,EPCsとECsが共に管腔を形成することが確認された.マイクロ流路を用いたEPCsの循環動態観察では,壁面をEPCsがローリングしながら流れていく様子が観察され,生体内での微小循環におけるEPCs動態を生体外で再構築できると考えている。さらに,これまでの研究において我々は,アディポネクチンがPI3K/Rac1/Cdc42の経路を介してEPCsの遊走能を有意に増加させることを明らかにしたが,アディポネクチンを添加するとEPCsにおけるラメリポディア形成が有意に増加することも明らかとなった.これらの結果は,すべてEPCsの血管発生,血管修復の一部の過程を観察していると考えられ,今後これらを統合したEPCsの働きを観察するシステムを開発していきたい.

Report

(2 results)
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (30 results)

All 2009 2008 2005

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (14 results)

  • [Journal Article] Polymorphism in resistin promoter region at -420 determines the serum resistin levels and may be a risk marker of stroke in Japanese type 2 diabetic patients2009

    • Author(s)
      Takayoshi Tsukahara
    • Journal Title

      Diabetes Research and Clinical Practice 84

      Pages: 179-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac12009

    • Author(s)
      Nobuhisa Nakamura
    • Journal Title

      FEBS letters 583

      Pages: 2457-2463

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma resistin concentration determined by common variants in the resistin gene and associated with metabolic traits in an aged Japanese population2009

    • Author(s)
      Hiroyuki Asano
    • Journal Title

      Diabetologia 53

      Pages: 234-246

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of long-term statin treatment on coronary plaque composition at angiograp hically severe lesions : a nonrandomized study of the history of long-term statin treatment before coronary angioplasty2009

    • Author(s)
      Motohiro Miyagi
    • Journal Title

      Clinical Therapeutics 31

      Pages: 64-73

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracoronary electrocardiogram recording with a bare-wire system : perioperative ST-segment elevation in the intracoronary electrocardiogram is associated with myocardial injury after elective coronary stent implantation2009

    • Author(s)
      Tadayuki Uetani
    • Journal Title

      JACC : Cardiovascular Interventions 2

      Pages: 127-135

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic values of C-reactive protein levels on clinical outcome after implantation of sirolimus-eluting stents in patients on hemodialysis2009

    • Author(s)
      Hideki Ishii
    • Journal Title

      Circulation : Cardiobascular Interventions 2

      Pages: 513-518

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of Bone Marrow-derived Mesenchymal Stem Cells Improves Diabetic Polvneuropathy in Rats2008

    • Author(s)
      Taiga Shibata
    • Journal Title

      Diabetes 57

      Pages: 3099-3107

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High glucose-induced upregulation of Rho / Rho-kinase via platelet- derived growth factor receptor-beta increases migration of aortic smooth muscle cells2008

    • Author(s)
      Noboru Akiyama
    • Journal Title

      Journal of Molecular and Cellular Cardiology 45

      Pages: 326-332

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced NGF secretion by Schwann cells under the high glucose condition decreases neurite outgrowth of DRG neurons2008

    • Author(s)
      Takahiro Tosaki
    • Journal Title

      Experimental Neurology 213

      Pages: 381-387

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Na^<+> - independent Mg^<2+> transport sensitive to 2-aminoethoxydiphenyl borate (2-APB) in vascular smooth muscle cells : involvement of TRPM-lika channels2008

    • Author(s)
      Yukihisa Hamaguchi
    • Journal Title

      Journal of Cellular and Molecular Medicine 12

      Pages: 962-974

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stratified analyses for selecting appropriate target patients with diabetic peripheral neuropathy for long-term treatment with an aldose reductase inhibitor, epalrestat2008

    • Author(s)
      Nigishi Hotta
    • Journal Title

      Diabetic medicine 25

      Pages: 818-825

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association between future events of brain infarction and soluble levels of intercellular adhesion molecule-1 and C-reactive protein in patients with type 2 diabetes mellitus2008

    • Author(s)
      Akiko Kanai
    • Journal Title

      Diabetes research and clinical practice 25

      Pages: 157-164

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cilostazol improves long-term patency after percutaneous transluminal angioplasty in hemodialysis patients with peripheral artery disease2008

    • Author(s)
      Hideki Ishii
    • Journal Title

      Clinical Journal of American Society of Nephrology 3

      Pages: 1034-1040

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of reduced coronary flow reserve on left ventricular function in type 2 diabetes2008

    • Author(s)
      Osamu Yonaha
    • Journal Title

      Diabetes research and clinical practice 82

      Pages: 98-103

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antiglycation Effect of Gliclazide on In Vitro AGE Formation from Glueose and Methylglyoxal2005

    • Author(s)
      Weiguo Li
    • Journal Title

      Experimental Biology and Medicine 233

      Pages: 176-179

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacological intervention for prevention of left ventricular remodeling and imnroving in mvocardial infarction2005

    • Author(s)
      Hideki Ishii
    • Journal Title

      Circulation 118

      Pages: 2710-2718

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 糖尿病性神経障害の重要性~病態・治療と今後の展望~2009

    • Author(s)
      成瀬桂子
    • Organizer
      Nagoya Ophthalmic week 2009
    • Place of Presentation
      名古屋
    • Year and Date
      2009-12-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 糖尿病性神経障害に対する前駆細胞/幹細胞を用いた細胞治療2009

    • Author(s)
      成瀬桂子
    • Organizer
      第24回日本糖尿病合併症学会
    • Place of Presentation
      岡山
    • Year and Date
      2009-10-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] The functions of bone marrow-derived mononuclear cells and their therapeutic efficacy on diabetic polyneuropathy are affected by their age2009

    • Author(s)
      近藤正樹
    • Organizer
      45th European Association for the Study of Diabetes
    • Place of Presentation
      ウィーン
    • Year and Date
      2009-09-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 2型糖尿病患者において酸化ストレスは頸動脈肥厚と関連する2009

    • Author(s)
      小林泰子
    • Organizer
      第52回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 2型糖尿病患者における末梢血単核球のアルドース還元酵素遺伝子発現量は頸動脈病変と相関する2009

    • Author(s)
      成瀬桂子
    • Organizer
      第52回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] レジスチンによる単球接着能の亢進とそのメカニズムに関する研究2009

    • Author(s)
      松木孝
    • Organizer
      第52回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 糖尿病性末梢神経障害に対する骨髄単核球移植治療の効果-ドナーラットの性状相違による治療効果への影響2009

    • Author(s)
      近藤正樹
    • Organizer
      第52回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 糖尿病における血管内皮前駆細胞障害は、動脈硬化と関連する2008

    • Author(s)
      成瀬桂子
    • Organizer
      第23回日本糖尿病合併症学会
    • Place of Presentation
      東京
    • Year and Date
      2008-10-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Circulating Endothelial Progenitor Cells Are Associated with Vascular Disorder in Healthy and Type 2 Diabetic Subiects2008

    • Author(s)
      Keiko Naruse
    • Organizer
      第28回アメリカ糖尿病学会
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2008-06-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] Basic Fibroblast Growth Factor Reverses Established Diabetic Polyneuropathy in Rats2008

    • Author(s)
      Hideli Kamiya
    • Organizer
      第28回アメリカ糖尿病学会
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2008-06-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 糖尿病神経障害に対する幹/前駆細胞を用いた細胞治療2008

    • Author(s)
      成瀬桂子
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生体肝移植手術による肝硬変合併糖尿病患者の耐糖能改善効果と機序の検討2008

    • Author(s)
      中島英太郎
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] レジスチンによる単球の接着能に及ぼす影響および作用機序の検討2008

    • Author(s)
      松木孝
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Adiponectinは血管内皮前駆細胞の遊走能を促進する2008

    • Author(s)
      中村信久
    • Organizer
      第51回日本糖尿病学会年次学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-22
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2018-03-28  

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