Dynamics of Nuclear Architecture and Genome Organization during Neuronal Differentiation

• Project/Area Number: 20200009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general; Nanomaterials/Nanobioscience
• Research Institution: Osaka University
• Principal Investigator: SUGO Noriyuki Osaka University, 大学院・生命機能研究科, 助教 (20372625)
• Co-Investigator(Kenkyū-buntansha): ARAI Yoshiyuki 大阪大学, 大学院・生命機能研究科, 特任助教 (20444515)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥33,020,000 (Direct Cost: ¥25,400,000、Indirect Cost: ¥7,620,000); Fiscal Year 2010: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2008: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
• Keywords: 分子・細胞神経科学 / 1分子生理・生化学 / 転写因子 / 大脳皮質 / クロマチン / DNA修復

Research Abstract

Dynamics between multiple nuclear factors and genomic DNA in the nucleus during neuronal differentiation remain undiscovered. Here, we quantitatively observed dynamics of some nuclear proteins and genomic loci by fluorescent imaging in vitro and in vivo. As a result, we can detect an interaction between a transcription factor and its recognition sequence at the single-molecule level.

Research Products

• [Journal Article] A Molecular Mechanism That Regulates Medially Oriented Axonal Growth of Upper Layer Neurons in the Developing Neocortex. (2011)
  • Author(s): Hong Zhao, Takuro Maruyama, Yuki Hattori, Noriyuki Sugo, Hyota Takamatsu, Atsushi Kumanogoh, Ryuichi Shirasaki, Nobuhiko Yamamoto
  • Journal Title: J Comp Neurol. 519 Pages: 834-848
  • Peer Reviewed
• [Journal Article] A Molecular Mechanism That Regulates Medially Oriented Axonal Growth of Upper Layer Neurons in the Developing Neocortex (2011)
  • Author(s): Hong Zhao, et al.
  • Journal Title: The Journal of Comparative Neurology Volume: 519 Pages: 834-848
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons. (2010)
  • Author(s): Noriyuki Sugo, Hiroaki Oshiro, Mitsuhiro Takemura, Toshiaki Kobayashi, Yusuke Kohno, Naofumi Uesaka, Wen-Jie Song, Nobuhiko Yamamoto
  • Journal Title: Eur J Neurosci. 31 Pages: 1521-1532
  • Peer Reviewed
• [Journal Article] Self-organization of the phosphatidylinositol lipids signaling system for random cell migration. (2010)
  • Author(s): Yoshiyuki Arai, Tatsuo Shibata, Satomi Matsuoka, Masayuki J Sato, Toshio Yanagida, Masahiro Ueda
  • Journal Title: Proc Natl Acad Sci USA 107 Pages: 12399-12404
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons (2010)
  • Author(s): Noriyuki Sugo, et al.
  • Journal Title: European Journal of Neuroscience Volume: 31 Pages: 1521-1532
  • Peer Reviewed
• [Presentation] 発生期大脳皮質の前駆細胞においてDNA修復酵素は転写活性と連動して集積する (2010)
  • Author(s): 菅生紀之,至田充宏,山本亘彦
  • Organizer: 第33回日本神経科学大会,(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] DNA repair enzymes form foci in cortical progenitor cells in association with transcription (2010)
  • Author(s): Noriyuki Sugo, et al.
  • Organizer: Neuro2010 (第33回日本神経科学大会)
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-02
• [Presentation] Self-organization in phosphatidylinositol lipids signalling pathway for random cell migration and polarization. (2009)
  • Author(s): Yoshiyuki Arai, Tatsuo Shibata, Satomi Matsuoka, Masayuki J Sato, Toshio Yanagida, Masahiro Ueda
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Activity-dependent nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth indeveloping cortical neurons. (2009)
  • Author(s): Noriyuki Sugo, Hiroaki Oshiro, Mitsuhiro Takemura, Toshiaki Kobayashi, Yusuke Kohno, Naofumi Uesaka
  • Organizer: Construction and Reconstruction of theBrain
  • Place of Presentation: Awaji Yumebutai
  • Year and Date: 2009-10-09
• [Presentation] Activity-dependent nucleocytoplasmic translocation of HDAC9 regulates gene expression and dendritic growth in developing cortical neurons (2009)
  • Author(s): Noriyuki Sugo, et al.
  • Organizer: Construction and Reconstruction of the Brain
  • Place of Presentation: 淡路夢舞台(兵庫県)
  • Year and Date: 2009-10-09
• [Remarks] ホームページ等
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/