Elucidation of mechanisms for determination of retinal ganglion cell subtype and regular mosaic formation

• Project/Area Number: 20200010
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general; Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: SAKUTA Hiraki National Institute for Basic Biology, 統合神経生物学研究部門, 助教 (40343743)
• Co-Investigator(Renkei-kenkyūsha): YONEHARA Keisuke 基礎生物学研究所, 統合神経生物学研究部門, 特別協力研究員 (80510619)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥29,900,000 (Direct Cost: ¥23,000,000、Indirect Cost: ¥6,900,000); Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2008: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: 網膜神経節細胞 / サブタイプ / モザイク / マイクロアレイ / マウス

Research Abstract

For the first time, we succeeded in visualization of two distinct subtypes of retinal ganglion cell. For the purpose of elucidation of mechanisms for determination of retinal ganglion cell subtype and regular mosaic formation, we tried isolation of the subtype-specific genes using single-cell microarray method but failed. However, we succeeded in isolation of a gene which is commonly expressed in these two retinal ganglion cell subtypes. We revealed the electrophysiological property of the two subtypes. Furthermore, we found that these the two subtypes projected to the medial terminal nucleus via different pathways.

Research Products

• [Journal Article] Functional mode of FoxD1/CBF2 for the establishment of temporal retinal specificity in the developing chick retina. (2009)
  • Author(s): Takahashi, H., Sakuta, H., Shintani, T., Noda, M.
  • Journal Title: Dev Biol 331 Pages: 300-310
  • Peer Reviewed
• [Journal Article] Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion. (2009)
  • Author(s): Yonehara, K., Ishikane, H., Sakuta, H., Shintani, T., Nakamura-Yonehara, K., Kamiji, N.L., Usui, S., Noda, M.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Functional mode of FoxD1/CBF2 for the establishment of temporal retinal specificity in the developing chick retina (2009)
  • Author(s): Takahashi, H.et al.
  • Journal Title: Dev.Biol. 331 Pages: 300-310
  • Peer Reviewed
• [Journal Article] APC2 plays an essential role in axonal projections through the regulation of microtubule stability (2009)
  • Author(s): Shintani, T.et al.
  • Journal Title: J.Neurosci. 29 Pages: 11628-11640
  • Peer Reviewed
• [Journal Article] Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion (2009)
  • Author(s): Yonehara, K.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation. (2008)
  • Author(s): Sakuta, H., Suzuki, R., Noda, M.
  • Journal Title: Dev Growth Differ 50 Pages: 453-457
• [Journal Article] Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation (2008)
  • Author(s): Sakuta, H.
  • Journal Title: Dev. Growth Differ. 50 Pages: 453-457
• [Presentation] SPIG1の発現により明らかになったON中心型方向選択性神経節細胞サブタイプの発達様式 (2008)
  • Author(s): 米原圭祐、新谷隆史、鈴木亮子、作田拓、竹内靖、中村-米原佳世、石金浩史、Nilton L.Kamiji、臼井支朗、野田昌晴
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Book] In Electroporation and Sonoporation in Developmental Biology (H.Nakamura, ed.)(Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation.) (2009)
  • Author(s): Sakuta, H., Suzuki, R., Noda, M.
  • Publisher: Springer, Springer-Verlag
• [Book] In Encyclopedia of Neuroscience 4th ed.(L.M.Squire et al., eds.)(Neural Patterning : Eye fields.) (2009)
  • Author(s): Noda, M., Takahashi, H., Sakuta, H.
  • Publisher: Elsevier B.V., Amsterdam
• [Book] Electroporation and Sonoporation in Developmental Biology (2009)
  • Author(s): Sakuta, H.
  • Publisher: Springer
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