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Elucidation of mechanisms for determination of retinal ganglion cell subtype and regular mosaic formation

Research Project

Project/Area Number 20200010
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Neuroscience in general
Developmental biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

SAKUTA Hiraki  National Institute for Basic Biology, 統合神経生物学研究部門, 助教 (40343743)

Co-Investigator(Renkei-kenkyūsha) YONEHARA Keisuke  基礎生物学研究所, 統合神経生物学研究部門, 特別協力研究員 (80510619)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥29,900,000 (Direct Cost: ¥23,000,000、Indirect Cost: ¥6,900,000)
Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2008: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords網膜神経節細胞 / サブタイプ / モザイク / マイクロアレイ / マウス
Research Abstract

For the first time, we succeeded in visualization of two distinct subtypes of retinal ganglion cell. For the purpose of elucidation of mechanisms for determination of retinal ganglion cell subtype and regular mosaic formation, we tried isolation of the subtype-specific genes using single-cell microarray method but failed. However, we succeeded in isolation of a gene which is commonly expressed in these two retinal ganglion cell subtypes. We revealed the electrophysiological property of the two subtypes. Furthermore, we found that these the two subtypes projected to the medial terminal nucleus via different pathways.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (1 results) Book (3 results) Remarks (5 results)

  • [Journal Article] Functional mode of FoxD1/CBF2 for the establishment of temporal retinal specificity in the developing chick retina.2009

    • Author(s)
      Takahashi, H., Sakuta, H., Shintani, T., Noda, M.
    • Journal Title

      Dev Biol 331

      Pages: 300-310

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion.2009

    • Author(s)
      Yonehara, K., Ishikane, H., Sakuta, H., Shintani, T., Nakamura-Yonehara, K., Kamiji, N.L., Usui, S., Noda, M.
    • Journal Title

      PLoS ONE 4

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional mode of FoxD1/CBF2 for the establishment of temporal retinal specificity in the developing chick retina2009

    • Author(s)
      Takahashi, H.et al.
    • Journal Title

      Dev.Biol. 331

      Pages: 300-310

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] APC2 plays an essential role in axonal projections through the regulation of microtubule stability2009

    • Author(s)
      Shintani, T.et al.
    • Journal Title

      J.Neurosci. 29

      Pages: 11628-11640

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion2009

    • Author(s)
      Yonehara, K.
    • Journal Title

      PLoS ONE 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation.2008

    • Author(s)
      Sakuta, H., Suzuki, R., Noda, M.
    • Journal Title

      Dev Growth Differ 50

      Pages: 453-457

    • Related Report
      2010 Final Research Report
  • [Journal Article] Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation2008

    • Author(s)
      Sakuta, H.
    • Journal Title

      Dev. Growth Differ. 50

      Pages: 453-457

    • Related Report
      2008 Annual Research Report
  • [Presentation] SPIG1の発現により明らかになったON中心型方向選択性神経節細胞サブタイプの発達様式2008

    • Author(s)
      米原圭祐、新谷隆史、鈴木亮子、作田拓、竹内靖、中村-米原佳世、石金浩史、Nilton L.Kamiji、臼井支朗、野田昌晴
    • Organizer
      第31回日本神経科学大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Book] In Electroporation and Sonoporation in Developmental Biology (H.Nakamura, ed.)(Retroviral vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation.)2009

    • Author(s)
      Sakuta, H., Suzuki, R., Noda, M.
    • Publisher
      Springer, Springer-Verlag
    • Related Report
      2010 Final Research Report
  • [Book] In Encyclopedia of Neuroscience 4th ed.(L.M.Squire et al., eds.)(Neural Patterning : Eye fields.)2009

    • Author(s)
      Noda, M., Takahashi, H., Sakuta, H.
    • Publisher
      Elsevier B.V., Amsterdam
    • Related Report
      2010 Final Research Report
  • [Book] Electroporation and Sonoporation in Developmental Biology2009

    • Author(s)
      Sakuta, H.
    • Publisher
      Springer
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.nibb.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks] 所属機関ホームページ

    • URL

      http://niwww3.nibb.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://niwww3.nibb.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://niwww3.nibb.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://niwww3.nibb.ac.jp/

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2018-03-28  

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