Kidney regeneration using autologous stem cells

• Project/Area Number: 20200018
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine; Biomedical engineering/Biological material science
• Research Institution: Jikei University School of Medicine
• Principal Investigator: YOKOO Takashi Jikei University School of Medicine, 医学部, 講師 (70301538)
• Co-Investigator(Kenkyū-buntansha): UECHI Masami 日本大学, 生物資源科学部, 准教授 (90296426) ; OONISHI Akira 独立行政法人農業生物資源研究所, 遺伝子組換え家畜センター, 副研究主幹 (30414890)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥32,890,000 (Direct Cost: ¥25,300,000、Indirect Cost: ¥7,590,000); Fiscal Year 2010: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2009: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2008: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
• Keywords: 間葉系幹細胞 / 腎臓再生 / 異種胎仔 / 異種移植 / 胎仔

Research Abstract

Significant advances have been made in stem cell research over the past decade. A number of non-hematopoietic sources of stem cells (or progenitor cells) have been identified including endothelial stem cells and neural stem cells. These discoveries have been a major step towards the potential regeneration of organs for clinical applications using stem cells. The worldwide shortage of donor kidneys means that this approach has garnered significant attention in the field of nephrology. We challenged to use autologous stem cells to build kidney using developmental program of xeno-embryo.

Research Products

• [Journal Article] A thermoreversible polymer mediates controlled release of GDNF to enhance kidney regeneration. (2010)
  • Author(s): Gheisari Y, Yokoo T, Matsumoto K, Fukui A, Sugimoto N, Ohashi T, Kawamura T, Hosoya T, Kobayashi E.
  • Journal Title: Artif Organs 34 Pages: 317-331
• [Journal Article] A thermoreversible polymer mediates controlled release of GDNF to enhance kidney regeneration (2010)
  • Author(s): Gheisari Y, Yokoo T, et al
  • Journal Title: Artif Organs Volume: 34 Pages: 317-331
  • Peer Reviewed
• [Journal Article] Xenobiotic kidney organogenesis -A new avenue for renal transplantation- (2009)
  • Author(s): Yokoo T, Kawamura T
  • Journal Title: J.Nephrology 22 Pages: 312-317
• [Journal Article] Integration of human mesenchymal stem cells into the Wolffian duct in chicken embryos. (2009)
  • Author(s): Fukui A, Yokoo T, Matsumoto K, Kawamura T, Hosoya T, Okabe M
  • Journal Title: Biochem Biophys Res Commun 385 Pages: 330-335
• [Journal Article] Integration of human mesenchymal stem cells into the Wolffian duct in chicken embryos. (2009)
  • Author(s): Fukui A, Yokoo T, et al.
  • Journal Title: Biochem Biophys Res Commun 385 Pages: 330-335
  • Peer Reviewed
• [Journal Article] Stem cells for kidney repair: Useful tool for acute renal failure? (2008)
  • Author(s): Yokoo T, Kawamura T, Kobayashi E.
  • Journal Title: Kidney Int 74 Pages: 847-849
• [Journal Article] Generation of transplantable erythropoietin-producer derived from human mesenchymal stem cells. (2008)
  • Author(s): Yokoo T, Fukui A, Matsumoto K, Ohashi T, Sado Y, Kawamura T, Okabe M, Hosoya T, Kobayashi E.
  • Journal Title: Transplantation 85 Pages: 1654-1658
• [Journal Article] Stem cells and kidney organogenesis. (2008)
  • Author(s): Yokoo T, Fukui A, Okabe M, Kobayashi E
  • Journal Title: Frontiers in Bioscience 13 Pages: 2814-2832
• [Journal Article] Generation of transplantable erythropoietin-producer derived from human mesenchymal stem cells (2008)
  • Author(s): Yokoo T, et.al.
  • Journal Title: Transplantation 85 Pages: 1654-1658
  • Peer Reviewed
• [Journal Article] Potential use of stem cells for kidney regeneration.
  • Author(s): Yokoo T, Matsumoto K, Yokote S
  • Journal Title: International Journal of Nephrology (in press)
• [Journal Article] Functional development of a transplanted embryonic kidney : Effect of transplantation site.
  • Author(s): Matsumoto K, Yokoo T, Yokote S, Kawamura T, Utsunomiya Y, Ohashi T, Hosoya T.
  • Journal Title: J.Nephrol. (in press)
• [Presentation] ブタをscaffoldとする腎臓再生IVエリスロポエチン産生組織の体内発生法の開発 (2011)
  • Author(s): 横尾隆、松成ひとみ、岩井聡美、松本啓、辻収彦、岡野James洋尚、岡野栄之、長嶋比呂志、小林英司
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] ブタをscaffoldとする腎臓再生III,ネコにおける新規エリスロポエチン補充療法の開発 (2011)
  • Author(s): 岩井聡美、横尾隆、松成ひとみ、田中友加、寺岡善布史、大段秀樹、長嶋比呂志、小林英司
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] ブタをscaffoldとする腎臓再生III. Suicidegeneを発現する遺伝子改変ブタの作出 (2011)
  • Author(s): 松成ひとみ、横尾隆、岩井聡美、渡邊將人、梅山一大、中野和明、前原美樹、Medin JA、長嶋比呂志、小林英司
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] ブタをscaffoldとする腎臓再生I.クローンブタを利用した腎臓原基の発達能の検証 (2011)
  • Author(s): 長嶋比呂志、松成ひとみ、横尾隆、岩井聡美、小林英司
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-01
• [Presentation] Transplantation of metanephros suppresses the vascular calcification in rats with adenine-induced renal failure. (2010)
  • Author(s): Yokote S, Yokoo T, Matsumoto K, Ohkido I, Kawamura T, Hosoya T.
  • Organizer: American Society of Nephrology Renal Week
  • Place of Presentation: Denver CO, USA
  • Year and Date: 2010-11-19
• [Presentation] Use of the E2F1 transgenic suicide-inducible mice permit regeneration of completely human kidneys. (2010)
  • Author(s): Matsumoto K, Yokoo T, Yokote S, Kawamura T, Ohashi T, Hosoya T, Tsuji O, Okano JH, Okano H, Kobayashi E.
  • Organizer: American Society of Nephrology Renal Week
  • Place of Presentation: Denver CO, USA
  • Year and Date: 2010-11-18
• [Presentation] 教育講演2 腎臓再生による抜本的腎不全治療法の開発 (2010)
  • Author(s): 横尾隆
  • Organizer: 第62回日本泌尿器科学会西日本総会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-11-06
• [Presentation] 特別講演 再生医療と移植医療の融合による抜本的腎不全治療法の開発 (2010)
  • Author(s): 横尾隆
  • Organizer: 第14回北海道移植フォーラム
  • Place of Presentation: 札幌 北海道
  • Year and Date: 2010-07-10
• [Presentation] シンポジウムII 異種組織の適応現象を利用した腎臓再生法の開発 (2010)
  • Author(s): 横尾隆
  • Organizer: 第14回日本適応医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-03
• [Presentation] 特別講演II 透析回避を目的とした機能腎臓再生法の開発 (2010)
  • Author(s): 横尾隆
  • Organizer: 第22回小児腎臓病漢方研究会
  • Place of Presentation: 大阪
  • Year and Date: 2010-07-01
• [Presentation] シンポジウム2臨床応用に向けた腎臓再生。In vivoでの腎臓再生 (2010)
  • Author(s): 横尾隆
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-16
• [Presentation] MSC and their utility for renal failure (2010)
  • Author(s): Takashi Yokoo
  • Organizer: The 12^<th> Asian Pacific Congress of Nephrology
  • Place of Presentation: Soul Korea
  • Year and Date: 2010-06-07
• [Presentation] 特別講演「移植可能な実践的臓器再生法の開発」 (2010)
  • Author(s): 横尾隆
  • Organizer: 第22回神奈川移植医学会
  • Place of Presentation: 横浜 神奈川
  • Year and Date: 2010-05-15
• [Presentation] 医学と工学の融合による自己幹細胞由来腎臓再生法の実現化。シンポジウム1 臨床に繋がる再生医療の現状と近未来 (2010)
  • Author(s): 横尾隆
  • Organizer: 日本医工学治療学会 第26回学術大会
  • Year and Date: 2010-04-03
• [Presentation] 異種胎仔組織をもしいた再生腎臓誘導法の開発 (2010)
  • Author(s): 横尾隆、松成ひとみ、岩井聡美、松本啓、長嶋比呂志、小林英司
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場 広島
  • Year and Date: 2010-03-19
• [Presentation] 急性腎不全に陥った腎臓への胎仔由来組織の移植効果-バイオイメージング・ラットを用いた検討 (2010)
  • Author(s): 岩井聡美、横尾隆、杉本直美、松成ひとみ、長嶋比呂志、小林英司
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場 広島
  • Year and Date: 2010-03-19
• [Presentation] 胎仔腎臓原基の器官培養を利用した幹細胞スクリーニング-手技の仔細と蛍光画像パターンによる分類 (2010)
  • Author(s): 杉本直美、横尾隆、増田茂夫、花園豊、竹内賢吾、松成ひとみ、長嶋比呂志、土居雅子、小林英司
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場 広島
  • Year and Date: 2010-03-19
• [Presentation] iPS細胞迅速スクリーニング系としてのラット胎仔メタネフロン器官培養の可能性 (2010)
  • Author(s): 増田茂夫、杉本直美、竹内賢吾、横尾隆、小林英司、花園豊
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場 広島
  • Year and Date: 2010-03-18
• [Presentation] de novo organogenesis using mesenchymal stem cells. (2010)
  • Author(s): Yokoo T.
  • Organizer: Japan-Israel Workshop on Stem cells
  • Place of Presentation: Haifa Israel
  • Year and Date: 2010-02-23
• [Presentation] 「再生」シンポジウム1:代替治療の問題点と未来治療 (2009)
  • Author(s): 横尾隆
  • Organizer: 第10回腎不全病態治療研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-12-12
• [Presentation] ブタ胎仔発生系を用いた分化による臓器(腎・肝・膵)形成プロジェクトの提案 (2009)
  • Author(s): 横尾隆
  • Organizer: JSTr安全なiPS細胞を用いた創薬・診断・再生医療の実現に向けて」ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2009-05-27
• [Presentation] Physiological renin production from xeno-metanephros transplanted in the paraaortic area. (2009)
  • Author(s): Matsumoto K, Yokoo T, Fukui A, Tawamura T, Kobayashi E, Ohashi T, Hosoya T.
  • Organizer: World Congress of Nephrology
  • Place of Presentation: Milan Italy.
  • Year and Date: 2009-05-23
• [Presentation] Human mesenchymal stem cells can differentiate into the wolffian duct cells in chicken embryos. (2009)
  • Author(s): Fukui A, Yokoo T, Matsumoto K, Kawamura T, Hosoya T, Okabe M.
  • Organizer: World Congress of Nephrology
  • Place of Presentation: Milan Italy.
  • Year and Date: 2009-05-23
• [Presentation] ブタ後腎組織を足場とした自己骨髄由来腎臓再生法の開発 (2009)
  • Author(s): 横尾隆ら
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-16
• [Presentation] ブタ胎児を用いたヒト腎臓再生法の開発 特別講演II (2009)
  • Author(s): 横尾隆
  • Organizer: 第12回日本異種移植研究会
  • Place of Presentation: 鹿児島
  • Year and Date: 2009-03-07
• [Presentation] 腎臓の再生。シンポジウム13「移植医療と再生医療(1)」 (2009)
  • Author(s): 横尾隆、小林英司
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-06
• [Presentation] 腎臓再生医療の現状と期待 (2009)
  • Author(s): 横尾隆
  • Organizer: 第17回日本逆流性腎症フォーラム
  • Place of Presentation: 枚方市大阪
  • Year and Date: 2009-01-31
• [Presentation] ここまできた腎臓再生医療 (2009)
  • Author(s): 横尾隆
  • Organizer: 第30回動物臨床医学会記念年次大会 腎泌尿器分科会セミナー
  • Place of Presentation: 大阪国際会議場
• [Presentation] Creation of EPO producing tissue using Xeno-metanephros as a biocompetent scaffold in a bigger animal model for the clinical application. (2009)
  • Author(s): Yokoo T, Matsumoto K, Nagashima H, Matsunari H, Iwai S, Hosoya T, Kobayashi E.
  • Organizer: American Society of Nephrology Renal Week
  • Place of Presentation: San Diego CA USA
• [Presentation] Xeno-metanephros as a biocompetent scaffold for kidney regeneration. (2009)
  • Author(s): Yokoo T, Nagashima H, Matsunari H, Iwai S, Hosoya T, Kobayashi E
  • Organizer: IPTA-IXA
  • Place of Presentation: Venice Italy
• [Presentation] Kidney regeneration from mesenchymal stem cells using xeno-metanephros as a biocompetent scaffold. (2009)
  • Author(s): Yokoo T, Matsunari H, Nagashima H, Iwai S, Matsumoto K, Fukui A, Kawamura T, Hosoya T, Kobayashi E.
  • Organizer: World Congress of Nephrology
  • Place of Presentation: Milan Italy.
• [Presentation] Kidney regeneration from mesenchymal stem cells using xeno-metanephros as a biocompetent scaffold (2009)
  • Author(s): Yokoo T, et al
  • Organizer: World Congress of Nephrology 2009
  • Place of Presentation: Milan Italy.
• [Presentation] Advancement in Regenerative Medicine and Stem cell Applications : Kidney Regeneration. (2008)
  • Author(s): Yokoo T
  • Organizer: 6th Asia Pacific Cord Blood Bank Consortium.
  • Place of Presentation: Tokyo Japan
  • Year and Date: 2008-11-14
• [Presentation] Advancement in Regenerative Medicine and Stem cell Applications : Kidney Regeneration (2008)
  • Author(s): Yokoo T
  • Organizer: 6^<th> Asia Pacific Cord Blood Bank Conso rtium
  • Place of Presentation: Tokyo
  • Year and Date: 2008-11-14
• [Presentation] Closing remarks at Basic and Clinical Science Symposium "Bone marrow-derived cells and kidney diseases. (2008)
  • Author(s): Yokoo T
  • Organizer: American Society of Nephrology 2008 Renal Week
  • Place of Presentation: Philadelphia USA
  • Year and Date: 2008-11-05
• [Presentation] 腎臓をつくろうシンポジウム12挑戦!-移植可能な臓器を作る (2008)
  • Author(s): 横尾隆
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-21
• [Presentation] Kidney regeneration using organogenesis of xeno-embryo. (2008)
  • Author(s): Yokoo T.
  • Organizer: Satellite Symposium in Toronto University.
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-06-03
• [Presentation] Kidney regeneration by xeno-embryonic nephrogenesis. (2008)
  • Author(s): Yokoo T
  • Organizer: KIDSTEM International Conference
  • Place of Presentation: Liverpool UK
• [Presentation] Renal organogenesis across the mouse to rat xenogenic barrier under the tacrolimus monotherapy. (2008)
  • Author(s): Kotaro Kai, Takashi Yokoo, Takashi Murakami, Satoshi Teraoka, Eiji Kobayashi.
  • Organizer: 22nd International Congress of the Transplantation Society
  • Place of Presentation: Sydney Australia
• [Presentation] Application of non-viral GDNF diffusion for development of chimeric rat kidney with human components. (2008)
  • Author(s): Yokoo T, Fukui A, Matsumoto K, Hosoya T, Kobayashi E.
  • Organizer: The American Transplant Congress
  • Place of Presentation: Toronto, Canada
• [Presentation] Xeno-metanephros can be used as acaffold of producing host animal's erythropoietin.
  • Author(s): Matsumoto K, Yokoo T, Nagashima H, Matsunari H, Iwai S, Yokote S, Hosoya T, Ohashi T, Kobayashi E
  • Organizer: The 12th Asian Pacific Congress of Nephrology.
  • Place of Presentation: COEX Korea
• [Presentation] MSC and their utility for renal failure. 招待講演 Symposium 08 Acute Kidney Injury : Basic.
  • Author(s): Yokoo T
  • Organizer: The 12th Asian Pacific Congress of Nephrology.
  • Place of Presentation: COEX. Korea.
• [Presentation] ブタ後腎組織を足場とした自己骨髄由来腎臓再生法の開発
  • Author(s): 横尾隆、岩井聡美、長嶋比呂志、松成ひとみ、遠藤薫、上地正美、小林英司
  • Organizer: 第8回日本再生医療学会総会
• [Presentation] 透析回避に向けた腎臓再生医療の最前線
  • Author(s): 横尾隆
  • Organizer: 第38回近畿小児腎臓病研究会
  • Place of Presentation: 大阪
• [Book] Stem cell therapy against oxidative stress and hypoxia.(in Oxidative Stress in Basic Research and Clinical Practice)(edited by Miyata T, Echardt K-U, Nangaku M) (2011)
  • Author(s): Yokoo T, Yanagita M
  • Publisher: Springer New York, USA
• [Book] Oxidative Stress in Basic Research and Clinical Practice (2011)
  • Author(s): Yokoo T, Yanagita M
  • Publisher: Stem cell therapy against oxidative stress and hypoxia.
• [Book] Kidney development and regeneration in Stem cell : organogenesis and cancer (2010)
  • Author(s): Yokoo T, Fukui A, Kawamura T, Kobayashi E
  • Publisher: Research Signpost Press(In press)
• [Book] Chapter 4 : Kidney development and regeneration in Stem cell : organogenesis and cancer (edited by Shree Ram Singh)
  • Author(s): Yokoo T, Fukui A, Kawamura T, Kobayashi E.
  • Publisher: Research Signpost Press, Karala, India
• [Book] Renal stem cells and kidney regeneration in Regulatory Networks in Stem cells (edited by Rajasekhar VK and Vemuri M)
  • Author(s): Yokoo T, Fukui A, Matsumoto K, Kawamura T
  • Publisher: Humana Press INC., NJ USA
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