Development of DNA Sequencing Technologies Using Gating Nanopores

• Project/Area Number: 20200025
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience; Analytical chemistry
• Research Institution: Osaka University
• Principal Investigator: TANIGUCHI Masateru Osaka University, 産業科学研究所, 准教授 (40362628)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥31,330,000 (Direct Cost: ¥24,100,000、Indirect Cost: ¥7,230,000); Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000); Fiscal Year 2008: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: ナノボア / ゲーティングナノボア / DNAシーケンサー / 微細加工 / 単一分子計測 / トンネル電流 / イオン電流 / ナノポア / ゲーティングナノポア / メチル化DNA / 固体ナノポア / DNAシーケンシング / 1分子科学 / 生体分析 / ナノ加工 / 固体ナノポ / ゲーティング固体ナノポア / 金ナノ粒子 / 微小電流計測 / マイクロ流路

Research Abstract

Gating nanopores are the key devices for third generation DNA sequencing technologies. These nanodevices will make sequencing kilobase length single-stranded genomic DNA or RNA or identifying individual small molecules using only electric currents and without fluorescent labels at low cost and unheard speeds. We have developed vertical and parallel gating nanopores with embedded nanogap-electrodes in a solid-state nanopore. The vertical type consists of a single nanogap electrode with the nanopore perpendicular to the surface of the silicon substrate. The parallel type consists of a single nanogap electrode with the nanopore parallel to the surface of the substrate. We synthesized vertical gating nanopores with a diameter of 30 nm using an 11-step nanofabrication process. Vertical gating nanopores can indetify a single Au nanoparticle (~ = 28 nm) passing through them by changes in the electric current flowing between the nano-electrodes. Single base molecules of DNA can be identified by changes in tunneling current between nano-electrodes using parallel gating nanopores, incorporating a microfluidic channel in to nano-fabricated mechanically controllable break junction. We found that single-molecule electrical conductance order thymine < cytosine < adenine < guanine corresponds to the highest occupied molecular orbital (HOMO) energy order.

Research Products

• [Journal Article] Single Molecule Identification via Electric Current Noise (2011)
  • Author(s): Makusu Tsutsui, Masateru Taniguchi, Tomoji Kawai
  • Journal Title: Nature Communications 1 Pages: 138-142
  • Peer Reviewed
• [Journal Article] dentifying Single Nucleotides by Tunneling Current (2010)
  • Author(s): Makusu Tsutsui, Masateru Taniguchi, Kazumichi Yokota, Tomoji Kawai
  • Journal Title: Nature Nanotechnology 5 Pages: 286-290
  • Peer Reviewed
• [Journal Article] Single Molecule Identification via Electric Current Noise (2010)
  • Author(s): M.Tsutsui
  • Journal Title: Nature Communications Volume: 1 Pages: 138-142
  • Peer Reviewed
• [Journal Article] Identifying Single Nucleotides by Tunnelling Current (2010)
  • Author(s): M.Tsutsui
  • Journal Title: Nature Nanotechnology 5 Pages: 286-290
  • Peer Reviewed
• [Journal Article] Fabrication of the Gating Nanopore, Appl. (2009)
  • Author(s): Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Journal Title: Phys.Lett. 95 Pages: 123701-123703
  • Peer Reviewed
• [Journal Article] Atomistic Mechanics and Formation Mechanism of Metal-Molecule-Metal Junctions (2009)
  • Author(s): Makusu Tsutsui, Masateru Taniguchi, Tomoji Kawai
  • Journal Title: Nano Left. 9 Pages: 2433-2439
  • Peer Reviewed
• [Journal Article] Fabrication of the Gating Nanopore (2009)
  • Author(s): M.Taniguchi
  • Journal Title: Appl.Phys.Lett. 95 Pages: 123701-123703
  • Peer Reviewed
• [Journal Article] Transverse field effects on DNA-sized particle dynamics (2009)
  • Author(s): M. Tsutsui
  • Journal Title: Nano Letters (in press)
  • Peer Reviewed
• [Presentation] 核酸塩基分子の単分子識別-次々世代DNAシーケンサーにむけて- (2011)
  • Author(s): 谷口正輝
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-03-28
• [Presentation] 核酸塩基分子の単分子識別-次々世代DNAシーケンサーにむけて- (2011)
  • Author(s): 谷口正輝
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] 1分子解析技術による次々世代DNAシーケンサーの開発 (2011)
  • Author(s): 谷口正輝
  • Organizer: 日本表面科学会第69回表面科学研究会
  • Place of Presentation: 東京工業大学(東京都)
  • Year and Date: 2011-03-09
• [Presentation] 1分子解析技術による次々世代DNAシーケンサーの開発 (2011)
  • Author(s): 谷口正輝
  • Organizer: 日本表面科学会 第69回表面科学研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-09
• [Presentation] Development of Gating Nanopores for Single-Molecule Electrical Sequencing (2011)
  • Author(s): Masateru Taniguchi
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-01-24
• [Presentation] Development of Gating Nanopores for Single-Molecule Electrical Sequencing (2011)
  • Author(s): M.Taniguchi
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules
  • Place of Presentation: 京都
  • Year and Date: 2011-01-24
• [Presentation] Development of 3 D Generation DNA Sequencer Using Gating Nanopore Devices (2010)
  • Author(s): Masateru Taniguchi
  • Organizer: 2nd Japanese-Russian Young Scientists Conference on Nano-materials and Nano-technology
  • Place of Presentation: 東京工業大学(東京都)
  • Year and Date: 2010-09-22
• [Presentation] Development of 3 D Generation DNA Sequencer Using Gating Nanopore Devices (2010)
  • Author(s): M.Taniguchi
  • Organizer: 2nd Japanese-Russian Young Scientists Conference on Nano-materials and Nano-technology
  • Place of Presentation: 東京
  • Year and Date: 2010-09-22
• [Presentation] ゲーティングナノボアを用いた1分子検出 (2010)
  • Author(s): 谷口正輝
  • Organizer: バイオ・マイクロシステム研究会、電気学会研究会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2010-01-29
• [Presentation] Identification of Single Nucleotides Using Gating Nanopores (2010)
  • Author(s): Masateru Taniguchi
  • Organizer: 13th SANKEN International Symposium 2010
  • Place of Presentation: 関西空港ANAホテル(大阪府)
  • Year and Date: 2010-01-19
• [Presentation] Identification of Single Nucleotides Using Gating Nanopores (2010)
  • Author(s): M.Taniguchi
  • Organizer: 13th SANKEN International Symposium 2010
  • Place of Presentation: 関西空港(大阪府)
  • Year and Date: 2010-01-19
• [Presentation] ゲーティング固体ナノボアを用いた電気計測 (2009)
  • Author(s): 谷口正輝
  • Organizer: 第56回応用物理学関係連合講演会(招待講演)
  • Place of Presentation: 筑波大学(茨城県)
  • Year and Date: 2009-03-31
• [Presentation] ゲーティング固体ナノポアを用いた電気計測 (2009)
  • Author(s): 谷口正輝
  • Organizer: 第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学
  • Year and Date: 2009-03-31
• [Remarks] ホームページ
  • URL: http://www-kawai.sanken.osaka-u.ac.jp/