Development of genetically engineered animals by use of non-B DNA structure for highly and stably expression of transgene
| Project/Area Number |
20200032
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
Applied genomics
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| Research Institution | Kinki University |
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Principal Investigator |
MITANI Tasuku Kinki University, 先端技術総合研究所, 准教授 (10322265)
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| Co-Investigator(Kenkyū-buntansha) |
ANZAI Masayuki 近畿大学, 先端技術総合研究所, 准教授 (30454630)
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| Co-Investigator(Renkei-kenkyūsha) |
OHYAMA Takashi 早稲田大学, 教育・総合科学学術院, 教授 (60268513)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥33,540,000 (Direct Cost: ¥25,800,000、Indirect Cost: ¥7,740,000)
Fiscal Year 2010: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2008: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Keywords | 応用動物 / クロマチン / 発現制御 / 発生工学 / ES細胞 / 分化誘導 / RNAi |
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| Research Abstract |
This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows : T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] In vivo application of an RNAi strategy for the selective suppression of a mutant allele.2011
Author(s)
Kubodera T, Yamada H, Anzai M, Ohira S, Yokota S, Hirai Y, Mochizuki H, Shimada T, Mitani T, Mizusawa H, Yokota T.
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Journal Title
Human Gene Therapy 22
Pages: 27-34
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Peer Reviewed
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[Presentation] マウス胚性幹細胞の分化過程における細胞核染色体テリトリーと遺伝子座の動態2010
Author(s)
西山有依, 森木甲子郎, 藤本佑希, 安齋政幸, 加藤博己, 松本和也, 佐伯和弘, 入谷明, 細井美彦, 田辺秀之, 三谷匡
Organizer
第28回日本受精着床学会総会
Place of Presentation
横浜
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