| Budget Amount *help |
¥33,540,000 (Direct Cost: ¥25,800,000、Indirect Cost: ¥7,740,000)
Fiscal Year 2010: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2008: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Research Abstract |
This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows : T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.
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