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Development of genetically engineered animals by use of non-B DNA structure for highly and stably expression of transgene

Research Project

Project/Area Number 20200032
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Applied genomics
Research InstitutionKinki University

Principal Investigator

MITANI Tasuku  Kinki University, 先端技術総合研究所, 准教授 (10322265)

Co-Investigator(Kenkyū-buntansha) ANZAI Masayuki  近畿大学, 先端技術総合研究所, 准教授 (30454630)
Co-Investigator(Renkei-kenkyūsha) OHYAMA Takashi  早稲田大学, 教育・総合科学学術院, 教授 (60268513)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥33,540,000 (Direct Cost: ¥25,800,000、Indirect Cost: ¥7,740,000)
Fiscal Year 2010: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2008: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywords応用動物 / クロマチン / 発現制御 / 発生工学 / ES細胞 / 分化誘導 / RNAi
Research Abstract

This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows : T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (58 results)

All 2011 2010 2009 2008 Other

All Journal Article (19 results) (of which Peer Reviewed: 16 results) Presentation (35 results) Remarks (4 results)

  • [Journal Article] Competence of an artificial bent DNA as a transcriptional activator in the mouse ES cells.2011

    • Author(s)
      Tanase J, Mitani T, Udagawa K, Nishikawa J, Takashi Ohyama T.
    • Journal Title

      Mol.Biol.Reports 38

      Pages: 37-47

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo application of an RNAi strategy for the selective suppression of a mutant allele.2011

    • Author(s)
      Kubodera T, Yamada H, Anzai M, Ohira S, Yokota S, Hirai Y, Mochizuki H, Shimada T, Mitani T, Mizusawa H, Yokota T.
    • Journal Title

      Human Gene Therapy 22

      Pages: 27-34

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Competence of an artificial bent DNA as a transcriptional activator in the mouse ES cells.2011

    • Author(s)
      Tanase J
    • Journal Title

      Mol.Biol.Reports

      Volume: 38 Pages: 37-47

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo application of an RNAi strategy for the selective suppression of a mutant allele.2011

    • Author(s)
      Kubodera T
    • Journal Title

      Human Gene Therapy

      Volume: 22 Pages: 27-34

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice.2010

    • Author(s)
      Onodera Y, Teramura T, Ozawa M, Takehara T, Mitani T, Anzai M, Sagawa N, Hamanishi C, Hosoi Y, Fukuda K.
    • Journal Title

      Theriogenology 74

      Pages: 135-145

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deposition of acetylated histones by RNAP II promoter clearance may occur at onset of zygotic gene activation in preimplantation mouse embryos.2010

    • Author(s)
      Tokoro M
    • Journal Title

      J.Reprod.Dev.

      Volume: 56 Pages: 607-615

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of the ubiquitin-proteasome system leads to delay of the onset of ZGA gene expression.2010

    • Author(s)
      Shin SW
    • Journal Title

      J.Reprod.Dev.

      Volume: 56 Pages: 655-663

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice.2010

    • Author(s)
      Onodera Y
    • Journal Title

      Theriogenology

      Volume: 74 Pages: 135-145

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C57BL/6未成熟卵子を用いた成熟後におけるレーザー穿孔処理・体外受精方法の検討2010

    • Author(s)
      西村愛美
    • Journal Title

      近畿大学先端技術総合研究所紀要

      Volume: 15 Pages: 27-36

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Competence of an artificial bent DNA as a transcriptional activator in mouse ES cells2010

    • Author(s)
      Tanase J
    • Journal Title

      Mol.Biol.Rep. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silencing efficency differs among tissues and endogenous micro RNA pathway in preserved in short hairpin RNA transgenic mice.2009

    • Author(s)
      Sasaguri H, Mitani T, Anzai M, Kubodera T, Saito Y, Yamada H, Mizusawa H, Yokota T.
    • Journal Title

      FEBS Letters 583

      Pages: 213-218

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] マウス体細胞核移植由来再構築卵子における核内構造制御タンパク質の発現の解析2009

    • Author(s)
      西山有依, 森田真裕, 安齋政幸, 加藤博己, 細井美彦, 三谷匡, 入谷明
    • Journal Title

      Mem.Inst.Adv.Technol., Kinki Univ. 14

      Pages: 21-30

    • NAID

      120005736390

    • Related Report
      2010 Final Research Report
  • [Journal Article] Mouse androgenetic embryonic stem cells differentiated to multiple cell lineages in three embryonic germ layers in vitro2009

    • Author(s)
      Teramura T
    • Journal Title

      J.Reprod Dev. 55

      Pages: 283-292

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abnormal DNA methylation of the Oct-4 enhancer region in cloned mouse embryos2009

    • Author(s)
      Kawasumi M
    • Journal Title

      Mol.Reprod.Dev. 76

      Pages: 342-350

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silencing efficency differs among tissues and endogenous micro RNA pathway in preserved in short hairpin RNA transgenic mice2009

    • Author(s)
      Sasaguri H.
    • Journal Title

      FEBS Letters 583

      Pages: 213-218

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abnormal DNA methylation of the Oct-4 enhancer region in cloned mouse embryos2009

    • Author(s)
      Kawasumi M.
    • Journal Title

      Mol. Reprod. Dev 76

      Pages: 342-350

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マウス体細胞核移植由来再構築卵子における核内構造制御タンパク質の発現の解析2009

    • Author(s)
      西山有依
    • Journal Title

      近畿大学先端技術総合研究所紀要 14(印刷中)

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Isolation and culture of rabbit primordial germ cells2008

    • Author(s)
      Kakegawa R.
    • Journal Title

      J. Reprod. Dev 54

      Pages: 352-357

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone Hloo, Nucleoplasmin 2, and Zygote Arrest 1) are required for oocyte-specific gene expression in the mouse2008

    • Author(s)
      Tsunemoto K.
    • Journal Title

      Mol. Reprod. Dev 75

      Pages: 1104-1108

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] C57BL/6未成熟卵子を用いた同系凍結精子との体外受精および産子発生能に関する検討2011

    • Author(s)
      石束祐太
    • Organizer
      日本実験技術者協会 関東支部第36回懇話会
    • Place of Presentation
      府中グリーンプラザ(東京)
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス胚性幹細胞の分化誘導過程における細胞核染色体テリトリーと遺伝子座の動態2010

    • Author(s)
      西山有依, 川口翔, 平野大起, 森木甲子郎, 藤本佑希, 細井美彦, 田辺秀之, 三谷匡
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス胚性幹細胞の分化過程における細胞核染色体テリトリーと遺伝子座の動態2010

    • Author(s)
      西山有依, 森木甲子郎, 藤本佑希, 安齋政幸, 加藤博己, 松本和也, 佐伯和弘, 入谷明, 細井美彦, 田辺秀之, 三谷匡
    • Organizer
      第28回日本受精着床学会総会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス胚性幹細胞の分化誘導過程における細胞核染色体テリトリーと遺伝子座の動態2010

    • Author(s)
      西山有依
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス胚性幹細胞におけるABCトランスポーター・Bcrp1 mRNAアイソフォームAの転写調節領域の解析2010

    • Author(s)
      平野大起
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス初期胚における発生制御タンパク質の解析2010

    • Author(s)
      野老美紀子
    • Organizer
      第103回日本繁殖生物学会大会
    • Place of Presentation
      北里大学(十和田市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] C57BL/6未成熟卵子を用いた成熟後における同系凍結精子との体外受精成績2010

    • Author(s)
      西村愛美
    • Organizer
      第44回日本実験技術者協会総会
    • Place of Presentation
      旭川市民文化会館(旭川市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス胚性幹細胞の分化過程における細胞核染色体テリトリーと遺伝子座の動態2010

    • Author(s)
      西山有依
    • Organizer
      第28回日本受精着床学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Reconstitution of seminiferous tubules by xenoectopic transplantation of bovine testicular cells into the subcutis of immunodeficient mice.2010

    • Author(s)
      Mitani T
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2010 Annual Research Report
  • [Presentation] C57BL/6体外成熟卵子を用いた透明帯レーザー穿孔処理後における媒精濃度が体外受精成績にあたえる影響2010

    • Author(s)
      西村愛美
    • Organizer
      第51回日本哺乳動物卵子学会学術集会・総会
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 顕微授精により作製した2細胞期胚の低温輸送の検討2010

    • Author(s)
      西村愛美
    • Organizer
      第57回日本実験動物学会総会
    • Place of Presentation
      京都テルサ(京都市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス体細胞核移植由来卵子におけるクロマチンリモデリング複合体、SWR1複合体の構成因子の発現2009

    • Author(s)
      西山有依, 森田真裕, 安齋政幸, 加藤博己, 細井美彦, 原田昌彦, 三谷匡, 入谷明
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      奈良
    • Year and Date
      2009-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Expression of the components of chromatin remodeling factor SWR1 complex in mouse somatic nuclear transfer eggs.2009

    • Author(s)
      西山有依, 森木甲子郎, 森田真裕, 安齋政幸, 加藤博己, 細井美彦, 入谷明, 原田昌彦, 三谷匡
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamics of Arp family proteins and components of chromatin remodeling complexes in in vitro differentiation of mouse embryonic stem cells.2009

    • Author(s)
      森木甲子郎, 西山有依, 川村紘子, 安齋政幸, 加藤博己, 細井美彦, 原田昌彦, 入谷明, 三谷匡
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Stable expression of transgenes realized by chromatin engineering.2009

    • Author(s)
      棚瀬潤一, 宇田紘司, 西川純一, 三谷匡, 大山隆
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] マウスES細胞の未分化維持機構においてABCトランスポーターBcrp1が与える影響2009

    • Author(s)
      川村紘子, 川合智子, 田口善智, 安齋政幸, 加藤博己, 細井美彦, 三谷匡, 入谷明
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      奈良
    • Related Report
      2010 Final Research Report
  • [Presentation] Effects of ABC transporter, Bcrp1, on in vitro differentiation of mouse embryonic stem cells2009

    • Author(s)
      川村紘子
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dynamics of Arp family proteins and components of chromatin remodeling complexes in in vitro differentiation of mouse embryonic stem cells2009

    • Author(s)
      Moriki K
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Stable expression of transgenes realized by chromatin engineering2009

    • Author(s)
      Tanase J
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Expression of the components of chromatin remodeling factor SWR1 complex in mouse somatic nuclear transfer eggs2009

    • Author(s)
      西山有依
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウスES細胞の未分化維持機構においてABCトランスポーターBcrp1が与える影響2009

    • Author(s)
      川村紘子
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部(奈良市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス体細胞核移植由来卵子におけるクロマチンリモデリング複合体、SWR1複合体の構成因子の発現2009

    • Author(s)
      西山有依
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部(奈良市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 若齢ウシ精巣細胞を用いたヌードマウス皮下への異種異所移植による精子形成誘導の試み2009

    • Author(s)
      森木甲子郎
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部(奈良市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] プロテオミクスを用いたマウス初期胚における発生関連タンパク質の解析2009

    • Author(s)
      野老美紀子
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      近畿大学農学部(奈良市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウスES細胞の未分化維持機構においてABCトランスポーターBcrp1が与える影響2009

    • Author(s)
      川村紘子
    • Organizer
      第27回日本受精着床学会総会・学術講演会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 若齢ウシ精巣細胞を用いたヌードマウス皮下への異種異所移植による精子誘導の試み2009

    • Author(s)
      森木甲子郎
    • Organizer
      第27回日本受精着床学会総会・学術講演会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Reconstitution of seminiferous tubules in xenoectopic transplantation with bovine testicular cells2009

    • Author(s)
      Kita S
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      TOKI MESSE(Niigata)
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウスES細胞分化誘導過程におけるにABCトランスポーターBcrp1アイソフォームの発現様式およびRNAiによるアイソフォームAノックダウンES細胞樹立の試み2008

    • Author(s)
      川村紘子, 網本直記, 田口善智, 安齋政幸, 加藤博己, 細井美彦, 入谷明, 三谷匡
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] マウスES細胞の分化誘導過程におけるにABCトランスポーターBcrp1アイソフォームの発現様式2008

    • Author(s)
      川村紘子, 網本直記, 田口善智, 安齋政幸, 加藤博己, 細井美彦, 入谷明, 三谷匡
    • Organizer
      第26回日本受精着床学会総会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] Differentiation of mouse ES cells to hepatocytes and its application.2008

    • Author(s)
      Mitani T.
    • Organizer
      Recent Advance of Embryonic and Somatic Stem Cells in Biomedical Science Workshop.
    • Place of Presentation
      Bangkok, Thailand (Invited Speaker)
    • Related Report
      2010 Final Research Report
  • [Presentation] Expression of Bcrp1 mRNA isoforms during in vitro differentiation of mouse embryonic stem cells.2008

    • Author(s)
      Kawamura H, Amimoto N, Moriki K, Morita M, Anzai M, Kato H, Hosoi Y, Iritani A, Mitani T.
    • Organizer
      41st Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Tokushima, Japan.
    • Related Report
      2010 Final Research Report
  • [Presentation] マウスES細胞分化誘導過程におけるABCトランスポーターBcrp1アイソフォームの発現様式およびRNAiによるアイソフォームAノックダウンES細胞樹立の試み2008

    • Author(s)
      川村紘子
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] トリコスタチンA処理によるマウス体細胞核移植胚の着床初期における胚発生に関わる遺伝子発現の解析2008

    • Author(s)
      森田真裕
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス着床前期胚における大規模プロテオーム解析2008

    • Author(s)
      野老美紀子
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] トランスジェニックマウスを用いた母性遺伝子プロモーター解析2008

    • Author(s)
      畑中勇輝
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://rais.itp.kindai.ac.jp/researchdb/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://rais.itp.kindai.ac.jp/researchdb/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://rais.itp.kindai.ac.jp/researchdb/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://rais.itp.kindai.ac.jp/researchdb/

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2018-03-28  

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