Biosynthetic engineering of isoquinoline alkaloids in microbes
| Project/Area Number |
20200036
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
Living organism molecular science
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
MINAMI Hiromichi Ishikawa Prefectural University, 生物資源環境学部, 助教 (90433200)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Takane 石川県立大学, 生物資源環境学部, 准教授 (70346104)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Fumihiko 京都大学, 大学院・生命科学研究科, 教授 (10127087)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2010: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2009: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2008: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
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| Keywords | イソキノリンアルカロイド / レチクリン / マグノフロリン / 2次代謝産物 / テバイン / salutaridine synthase |
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| Research Abstract |
To improve the productivity of alkaloids in our microbial system, we modified the biosynthetic pathway and established the cytochrome P450 expression system in E. coli cells. Theses results enable the production of large amounts of alkaloids in E. coli cells. For the production of pharmaceutical important alkaloid narcotic, the biosynthetic genes were isolated from opium poppy, and were expressed in E. coli cells. We generated an E. coli strain that produce thebaine, introducing thebaine biosynthetic genes from reticuline in the reticuline producing E. coli cells. LC-MS analysis showed that the intermediates accumulated in the culture, but thebain was not detected. We have examined the production condition of thebaine.
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Report
(4 results)
Research Products
(26 results)
