| Project/Area Number |
20200039
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
Living organism molecular science
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| Research Institution | Tottori University |
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Principal Investigator |
KURIMASA Akihiro Tottori University, 大学院・医学系研究科, 准教授 (80343276)
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| Co-Investigator(Kenkyū-buntansha) |
NIWA Ohtaura 放射線医学総合研究所, 重粒子医科学センター, 副センター長 (80093293)
OKAYASU Ryuichi 放射線医学総合研究所, 重粒子医科学センター, グループリーダー (50356135)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥31,590,000 (Direct Cost: ¥24,300,000、Indirect Cost: ¥7,290,000)
Fiscal Year 2010: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2009: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2008: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
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| Keywords | 放射線治療生物学 / 分子標的創薬 / DNA修復 / RNA工学 / 生理活性物質 / RNAアプタマー / タンパク質リン酸化 / 癌治療 |
|---|
| Research Abstract |
It is well known that blocking protein kinases related to DNA repair machinery such as DNA-PKcs, ATM, or ATR enhance sensitivity to ionizing radiation (IR). From this point of view, we expect that tumor cell sensitivity against radiotherapy is enhanced by exploiting this mechanism. Protein kinases related to DNA repair machinery are activated by being phosphorylated. Therefore we attempted making the RNA engineering technology, and will develop new radiation sensitization medicine as a molecular target drug for cancer. In this experiment, we are making RNA aptamers against phosphorylated site of DNA-PKcs by SELEX. In this research, we produced RNA aptamers specific to DNA-PKcs phosphorylation site. Furtheremore their specific template sequences were analyzed by next-generation sequencing technology, and some that may recognize DNA-PKcs specifically were focused. U2OS cells are transfected with the RNA molecules, we tested whether can increase sensitivity of radiation. In conclusion, we found some RNA molecules that enhance sensitivity of radiation for U2OS cells.
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