| Budget Amount *help |
¥30,420,000 (Direct Cost: ¥23,400,000、Indirect Cost: ¥7,020,000)
Fiscal Year 2010: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2009: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2008: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Research Abstract |
We succeeded in synthesizing encephalomyocarditis virus in the human cell ext-derived in vitro protein synthesis system and purifying the virus. We developed the translation system reconstituted with human translation factors using the hepatitis C virus RNA, translation elongation factors, releasing factors, ribosomes and aminoacylated tRNAs. By using this system, we attempted to synthesize the capsid proteins of encephalomyocarditis virus.
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