An ingenious strategy for plant survival : Degradation of chloroplast proteins via autophagy and RCB vesicles

• Project/Area Number: 20200061
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science; Plant molecular biology/Plant physiology
• Research Institution: Tohoku University
• Principal Investigator: ISHIDA Hiroyuki 東北大学, 大学院・農学研究科, 准教授 (60312625)
• Co-Investigator(Kenkyū-buntansha): YOSIMOTO Kohki 理化学研究所, 植物免疫研究チーム, 基礎科学特別研究員 (40399316)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000); Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2008: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: 細胞死 / 老化 / 植物 / オートファジー / 葉緑体

Research Abstract

We studied mechanisms and physiological functions of a degradation of chloroplast proteins by autophagy as a strategy of plant survival. It was revealed that (i) tye production of RCBs which contain stromal proteins of chloroplasts is dependent on autophagy; (ii) the RCB production is closely related to leaf carbon status; (iii) autophagy negatively regulates leaf senescence by controlling salicylic acid signaling and the production of reactive oxygen species.

Research Products

• [Journal Article] The changes of leaf carbohydrate contents as a regulator of autophagic degradation of chloroplasts via Rubisco-containing bodies during leaf senescence (2011)
  • Author(s): Izumi M., Ishida H.
  • Journal Title: Plant Signal Behav. Volume: 6 (In press)
  • Peer Reviewed
• [Journal Article] The changes of leaf carbohydrate contents as a regulator of autophagic degradation of chloroplasts via Rubisco-containing bodies during leaf senescence (2011)
  • Author(s): Izumi, M., Ishida, H.
  • Journal Title: Plant Signal Behav. Volume: 6(In press)
  • Peer Reviewed
• [Journal Article] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi M., Wada S., Makino A., Ishida H.
  • Journal Title: Plant Physiol. Volume: 154 Pages: 1196-1209
  • Peer Reviewed
• [Journal Article] Plant autophagy puts the brakes on cell death by controlling salicylic acid signaling (2010)
  • Author(s): Yoshimoto K.
  • Journal Title: Autophagy Volume: 6 Pages: 192-193
  • Peer Reviewed
• [Journal Article] Biochemical change associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions (2010)
  • Author(s): Nakano R., Ishida H., Kobayashi M., Makino A., Mae T.
  • Journal Title: Plant Biol. Volume: 12 Pages: 35-45
  • Peer Reviewed
• [Journal Article] The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis (2010)
  • Author(s): Kwon S. I., Cho H. J., Jung J. H., Yoshimoto K., Shirasu K., Park O.
  • Journal Title: Plant J. Volume: 64 Pages: 151-164
  • Peer Reviewed
• [Journal Article] Physiological roles of autophagy in plants: Does plant autophagy have a pro-death fybctuib? (2010)
  • Author(s): Yoshimoto K.
  • Journal Title: Plant Signal Behav. Volume: 5 Pages: 194-196
  • Peer Reviewed
• [Journal Article] Autophagy in plants and phytopathogens (2010)
  • Author(s): Yoshimoto K., Takano Y., Sakai Y.
  • Journal Title: FEBS Let. Volume: 584 Pages: 1350-1358
  • Peer Reviewed
• [Journal Article] Role of chloroplasts and other plastids in ageing and death of plants and animals: A tale of Vishnu and Shiva (2010)
  • Author(s): Van Doom, W. G., Yoshimoto K.
  • Journal Title: Aging Res. Rev. Volume: 9 Pages: 117-130
  • Peer Reviewed
• [Journal Article] 植物におけるオートファジー研究の展開 (2010)
  • Author(s): 吉本光希
  • Journal Title: 植物の生長調節 Volume: 45 Pages: 24-32
  • NAID: 110007641950
• [Journal Article] Physiological roles of autophagy in plants : Does plant autophagy have a pro-death function? (2010)
  • Author(s): Yoshimoto, K.
  • Journal Title: Plant Signaling & Behavior Volume: 5 Pages: 194-496
  • Peer Reviewed
• [Journal Article] Role of chloroplasts and other plastids in ageing and death of plants and animals : A tale of Vishnu and Shiva (2010)
  • Author(s): Van Doom, W.G., Yoshimoto, K.
  • Journal Title: Aging Res.Rev. Volume: 9 Pages: 117-130
  • Peer Reviewed
• [Journal Article] Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions (2010)
  • Author(s): Nakano, R., Ishida, H., Kobayashi, M., Makino, A., Mae, T.
  • Journal Title: Plant Biol. Volume: 12 Pages: 35-45
  • Peer Reviewed
• [Journal Article] Plant autophagy puts the brakes on cell death by controlling salicylic acid signaling. (2010)
  • Author(s): Yoshimoto, K.
  • Journal Title: Autophagy Volume: 6 Pages: 192-193
  • Peer Reviewed
• [Journal Article] Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves (2009)
  • Author(s): Wada S., Ishida H., Izumi M., Yoshimoto K., et al
  • Journal Title: Plant Physiol. Volume: 149 Pages: 885-893
  • Peer Reviewed
• [Journal Article] Autophagy of whole and partial chloroplasts in individually darkened leaves: a unique system in plants? (2009)
  • Author(s): Ishida H., Wada S.
  • Journal Title: Autophagy Volume: 5 Pages: 736-737
  • Peer Reviewed
• [Journal Article] Chloroplasts autophagy during senescence of individually darkened leaves (2009)
  • Author(s): Wada S., Ishida H.
  • Journal Title: Plant Signal Behav. Volume: 4 Pages: 565-567
  • Peer Reviewed
• [Journal Article] Autophagy negatively regulates cell death by controlling NPR1-dependent salicylic acid signaling during senescence and innate immune response in Aravidopsis (2009)
  • Author(s): Yoshimoto K., Jikumaru Y., Kamiya Y., Kusano M., Consonni C., Panstruga R., Ohsumi Y., Shirasu K.
  • Journal Title: Plant Cell Volume: 21 Pages: 2914-2927
  • Peer Reviewed
• [Journal Article] Autophagy of whole and partial chloroplasts in individually darkened leaves : a unique system in plants? (2009)
  • Author(s): Ishida, H., Wada, S.
  • Journal Title: Autophagy Volume: 5 Pages: 736-737
  • Peer Reviewed
• [Journal Article] Autophagy negatively regulates cell death by controlling NPR1-dependent salicylic acid signaling during senescence and innate immune response in Arabidopsis. (2009)
  • Author(s): Yoshimoto, K., Jikumaru, Y., Kamiya, Y., Kusano, M., Consonni, C., Panstruga, R., Ohsumi, Y., Shirasu, K.
  • Journal Title: Plant Cell Volume: 21 Pages: 2914-2927
  • Peer Reviewed
• [Journal Article] Autophagy plays a role in chloroplast degradation during senescence in individually darkened leaves (2009)
  • Author(s): Wada, S., Ishida, H., Izumi, M., Yoshimoto, K, et.al.
  • Journal Title: Plant Physiology 149 Pages: 885-893
  • Peer Reviewed
• [Journal Article] Mobilization of Rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG gene-dependent autophagic process (2008)
  • Author(s): Ishida H., Yoshimoto K., et al.
  • Journal Title: Plant Physiol. Volume: 148 Pages: 142-155
  • Peer Reviewed
• [Journal Article] Chloroplasts are partially mobilized to the vacuole by autophagy (2008)
  • Author(s): Ishida H., Yoshimoto K.
  • Journal Title: Autophagy Volume: 4 Pages: 961-962
  • Peer Reviewed
• [Journal Article] Rubisco and Photosynthesis in cereal crops (2008)
  • Author(s): Makino A., Ishida H.
  • Journal Title: Tohoku Journal of Agricultural Research Volume: 58 Pages: 127-135
• [Journal Article] 葉緑体タンパク質の分解とオートファジー (2008)
  • Author(s): 石田宏幸, 和田慎也
  • Journal Title: 光合成研究 Volume: 53 Pages: 89-94
• [Journal Article] Mobilization of Rubisco and stroma-localized fluorescent proteins of chloroplasts to the vacuole by an ATG genede-pendent autophagic process (2008)
  • Author(s): Ishida, H., Yoshimoto, K, et.al.
  • Journal Title: Plant Physiology 148 Pages: 142-155
  • Peer Reviewed
• [Journal Article] Chloroplasts are partially mobilized to the vacuole by autophagy (2008)
  • Author(s): Ishida, H. and Yoshimoto, K
  • Journal Title: Autophagy 4 Pages: 961-962
  • Peer Reviewed
• [Journal Article] Rubisco and photosynthesis in cereal crops (2008)
  • Author(s): Makino, A. and Ishida, H
  • Journal Title: Tohoku Journal of Agricultural Research 58 Pages: 127-135
• [Journal Article] 葉緑体タンパク質の分解とオートファジー (2008)
  • Author(s): 石田宏幸、和田慎也
  • Journal Title: 光合成研究 53 Pages: 89-94
• [Presentation] オートファジーによる葉緑体タンパク質の分解 (2011)
  • Author(s): 石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナにおけるRBCS1Aおよび3Bの変異が葉のRubisco量に与える影響の解析 (2011)
  • Author(s): 泉正範, 角田穂奈美, 鈴木雄二, 牧野周, 石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] 植物オートファジーによる細胞死の制御 (2011)
  • Author(s): 吉本光希, 他
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] オートファジーによる葉緑体タンパク質の分解 (2011)
  • Author(s): 石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナにおけるRBCS1Aおよび3Bの変異が葉のRubisco量に与える影響の解析 (2011)
  • Author(s): 泉正範, 角田穂奈美, 鈴木雄二, 牧野周, 石田宏幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2011-03-22
• [Presentation] 植物オートファジーによる細胞死の制御 (2011)
  • Author(s): 吉本光希
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-03-22
• [Presentation] 老化・病原菌感染時における植物オートファジーの役割 (2010)
  • Author(s): 吉本光希, 他
  • Organizer: 日本植物学会
  • Place of Presentation: 春日井市
  • Year and Date: 2010-09-10
• [Presentation] Plant autophagy negatively regulates cell death by controlling salicylic acid signaling during senescence and the innate immune response (2010)
  • Author(s): Yoshimoto K., et al.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-07
• [Presentation] A key factor for the autophagy of chloroplasts; the production of Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi M., Makino A., Ishida H.
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-07
• [Presentation] A key factor for the autophagy of chloroplasts ; the production of Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi M., Makino A., Ishida H.
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-07
• [Presentation] Plant autophagy negatively regulates cell death by controlling salicylic acid signaling during senescence and the innate immune response (2010)
  • Author(s): 吉本光希
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-07
• [Presentation] オートファジー能欠損シロイヌナズナにおけるプログラム細胞死の原因は過剰なサリチル酸シグナリングである (2010)
  • Author(s): 吉本光希, 他
  • Organizer: 日本植物病理学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-20
• [Presentation] 植物オートファジーはサリチル酸シグナリングを抑制し細胞死に歯止めをかける (2010)
  • Author(s): 吉本光希, 軸丸裕介, 神谷勇治, 草野都, 大隅良典, 白須賢
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] シロイヌナズナにおけるデンプン代謝の変異がRCBの形成に及ぼす影響の解析 (2010)
  • Author(s): 泉正範, 石田宏幸, 牧野周
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナにおけるRubisco-containing body (RCB)形成の栄養要因に対する応答の解析 (2010)
  • Author(s): 泉正範, 牧野周, 石田宏幸
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 北海道
• [Presentation] A possible role for plastid stromules in the autophagic transfer of stromal proteins to the vacuole (2010)
  • Author(s): Ishida H., Izumi M., Yoshimoto K., Hanson M. R., Makino A.
  • Organizer: Gordon Research conference 'Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ, イタリア
• [Presentation] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi M., Makino A., Ishida H.
  • Organizer: Gordon Research conference 'Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ, イタリア
• [Presentation] The impact of autophagic degradation in chloroplasts and other organelles during leaf senescence of Arabidopsis (2010)
  • Author(s): Wada S., Ishida H., Yoshimoto K., Ohsumi Y., Makino A.
  • Organizer: Gordon Research conference 'Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ, イタリア
• [Presentation] A possible role for plastid stromules in the autophagic transfer of stromal proteins to the vacuole (2010)
  • Author(s): Ishida, H., Izumi, M., Yoshimoto, K., Hanson, M.R., Makino, A.
  • Organizer: Gordon Research conference 'Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ、イタリア
• [Presentation] The autophagic degradation of chloroplasts via Rubisco-containing bodies is specifically linked to leaf carbon status but not nitrogen status in Arabidopsis (2010)
  • Author(s): Izumi, M., Makino, A., Ishida, H.
  • Organizer: Gordon Research conference 'Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ、イタリア
• [Presentation] The impact of autophagic degradation in chloroplasts and other organelles during leaf senescence of Arabidopsis (2010)
  • Author(s): Wada, S., Ishida, H., Yoshimoto, K., Ohsumi, Y., Makino, A.
  • Organizer: Gordon Research conference 'Mitochondria and Chloroplasts'
  • Place of Presentation: バルガ、イタリア
• [Presentation] シロイヌナズナ切離葉における栄養要因がRCBの形成に及ぼす影響の解析 (2009)
  • Author(s): 泉正範, 石田宏幸, 牧野周
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-09-15
• [Presentation] シロイヌナズナ切離葉におけるRCBの形成に影響を及ぼす要因の解析 (2009)
  • Author(s): 泉正範, 石田宏幸, 牧野周
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] シロイヌナズナ個別暗処理葉におけるオートファジーに依存した葉緑体の分解 (2009)
  • Author(s): 和田慎也, 石田宏幸, 吉本光希, 他
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] シロイヌナズナの切離葉におけるRCBの形成に影響を及ぼす要因の解析 (2009)
  • Author(s): 泉正範、石田宏幸、牧野周
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] The role of autophagy in nutrient starvation and aging (2009)
  • Author(s): Yoshimoto K., Ishida H., et al.
  • Organizer: International Symposium on Autophagy
  • Place of Presentation: 大津プリンスホテル
• [Presentation] The analysis of factors affecting the creation of Rubisco-containing bodies (RCBs), a specific autophagic process for chloroplast degradation in plants (2009)
  • Author(s): Izumi M., Ishida H., Makino A.
  • Organizer: International Symposium on Autophagy
  • Place of Presentation: 大津プリンスホテル
• [Presentation] The chloroplast degradation in two different pathways in individually darkened leaves of Arabidopsis by autophagy (2009)
  • Author(s): Wada S., Ishida H., Yoshimoto K., et al.
  • Organizer: International Symposium on Autophagy
  • Place of Presentation: 大津プリンスホテル
• [Presentation] he chloroplast degradation in two different pathways in individually darkened leaves of Arabidopsis by autophagy (2009)
  • Author(s): Wada, S., Ishida, H., et al
  • Organizer: International Symposium on Autophagy
  • Place of Presentation: 大津プリンスホテル
• [Presentation] 膜小胞RCBを介した液胞におけるRubiscoの分解 (2008)
  • Author(s): 泉正範, 石田宏幸, 牧野周
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] シロイヌナズナの個葉・固体暗処理による老化誘導とオートファジーによる葉緑体分解 (2008)
  • Author(s): 和田慎也, 石田宏幸, 吉本光希, 他
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Presentation] シロイヌナズナの個葉・個体暗処理による老化誘導とオートファジーによる葉緑体分解 (2008)
  • Author(s): 和田慎也、石田宏幸、吉本光希, 他
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋市立大学
  • Year and Date: 2008-09-09
• [Book] Rubisco turnover and nitrogen in a leaf. Nitorgen assimilation in plants, Ohyama, T. and Sueyoshi, K. Eds., Chapter 19 (2010)
  • Author(s): Ishida H., Suzuki Y., Makino A.
  • Publisher: Research Sinpost, Kerala, India
• [Book] Protein turnover in grass leaves. Advances in Botanical Research, Vol. 54, Kader, J.C. and Delseny, M. Eds. (2010)
  • Author(s): Irving L. J., Suzuki Y., Ishida H., Makino A.
  • Publisher: Elsevier, Amsterdam, Netherlands