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Development of a new mass fragmentation analysis for understanding the diversity of metabolite

Research Project

Project/Area Number 20200062
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Applied biochemistry
Research InstitutionKazusa DNA Research Institute

Principal Investigator

SUZUKI Hideyuki  Kazusa DNA Research Institute, 産業基盤開発研究部, 主任研究員 (80276162)

Co-Investigator(Kenkyū-buntansha) AOKI Koh  財団法人かずさDNA研究所, 室長 (30344021)
SHIMADA Norimoto  財団法人かずさDNA研究所, プロジェクト研究員 (30409081)
櫻井 望  財団法人かずさDNA研究所, 産業基盤開発研究部, 研究員 (30392286)
飯島 陽子  財団法人かずさDNA研究所, 産業基盤開発研究部, 特任研究員 (90415456)
Co-Investigator(Renkei-kenkyūsha) OGATA Yoshiyuki  (独)理化学研究所, 植物科学研究センター, 研究員 (90446542)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥31,850,000 (Direct Cost: ¥24,500,000、Indirect Cost: ¥7,350,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2009: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2008: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords代謝物多様性 / 酸化反応 / メタボロミクス / 生物種間比較 / 進化 / 網羅的解析
Research Abstract

In order to understand the diversity of metabolites produced by organisms, we have investigated three topical studies focused on oxidative reaction of metabolite. 1) We attempted to isolate a cDNA of flavonoid 8-hydroxylase from Lotus japonicus. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. 2) We established a system to analyze metabolites found in Lotus japonicus that grows under oxygen-18 isotope and light conditions (16-h light/8-h dark cycles) at 22℃ for four weeks. 3) We developed a comprehensive metabolite network system based on MS/MS fragmentation analysis to classify the structural group of metabolites.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (4 results)

All 2011 2010 Other

All Presentation (2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] 同位体酸素環境下で生育した光合成生物の全代謝物の網羅的解析2011

    • Author(s)
      鈴木秀幸、嶋田典基、荒武、櫻井望、柴田大輔、青木考
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] ミヤコグサ由来フラボノイド8-水酸化酵素遺伝子の単離と機能解析2011

    • Author(s)
      嶋田典基、明石智義、青木俊夫、金森千奈、太田大策、青木考、柴田大輔、鈴木秀幸
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      仙台
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Remarks] ホームページ等なし

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 公開前につき非開示2010

    • Inventor(s)
      鈴木秀幸
    • Industrial Property Rights Holder
      サントリー株式会社
    • Industrial Property Number
      2010-183875
    • Filing Date
      2010-08-19
    • Related Report
      2010 Annual Research Report 2010 Final Research Report

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Published: 2008-04-01   Modified: 2018-03-28  

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