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In vivo induction of brown ad ipocyte differentiation by noninvasive delivery of miRNAs into stem cell

Research Project

Project/Area Number 20200064
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Developmental biology
Research InstitutionHokkaido University

Principal Investigator

KAJIMOTO Kazuaki  Hokkaido University, 大学院・薬学研究院, 特任准教授 (10416216)

Co-Investigator(Renkei-kenkyūsha) KATAOKA Masatoshi  独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (20224438)
TABUCHI Yoshiaki  富山大学, 生命科学先端研究センター, 准教授 (20322109)
SHINOHARA Yasuo  徳島大学, 疾患ゲノム研究センター, 教授 (60226157)
KOGURE Kentaro  京都薬科大学, 薬学部, 教授 (70262540)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥31,330,000 (Direct Cost: ¥24,100,000、Indirect Cost: ¥7,230,000)
Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2009: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2008: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywords褐色脂肪細胞 / miRNA / 発生・分化 / マイクロアレイ / 白色脂肪細胞 / モノクローナル抗体
Research Abstract

It was found that a lot of miRNAs were up-regulated when the subcutaneous adipose-derived stem cells (ASC) differentiated into brown-like adipocytes. Although these miRNAs may be involved in modulating ASC function, up-modulations of these miRNAs did not appear to affect ASC differentiation. In addition, we succeeded to obtain the novel monoclonal antibodies specifically responsible to ASC. Furthermore, we also applied the iontophoresis to noninvasive transdermal delivery of functional nucleic acids, and succeeded the silencing of the endogenous gene expression and activation of immune response in vivo. According to this study, it was suggested that ASC differentiation into brown-like adipocyte might be regulated by not only miRNAs but also unknown factors. Thus, the ASC specific antibodies and the delivery technology established by this study were helpful for the further investigation to clarify the molecular mechanisms of ASC function.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (8 results)

  • [Journal Article] Noninvasive and efficient transdermal delivery of CpG-oligodeoxynucleotide for cancer immunotherapy.2011

    • Author(s)
      Kigasawa K, Kajimoto K, Nakamura T, Hama S, Kanamura K, Harashima H, Kogure K.
    • Journal Title

      J Control Release. 150

      Pages: 256-265

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] イオントフォレシスによる高分子医薬の皮内送達2011

    • Author(s)
      小暮健太朗, 氣賀澤郁, 濱進, 梶本和昭
    • Journal Title

      薬剤学 71(2)

      Pages: 94-98

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis2011

    • Author(s)
      梶本和昭, 他6名
    • Journal Title

      International Journal of Pharmaceutics

      Volume: 403 Pages: 57-65

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Noninvasive and efficient transdermal delivery of CpG-oligodeoxynucleotide for cancer immunotherapy2011

    • Author(s)
      氣賀澤郁、梶本和昭, 他5名
    • Journal Title

      Journal of Controlled Release

      Volume: 150 Pages: 256-265

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis2010

    • Author(s)
      Kajimoto K, Yamamoto M, Watanabe M, Kigasawa K, Kanamura K, Harashima H, Kogure K.
    • Journal Title

      Int J Pharm. 403

      Pages: 57-65

    • NAID

      120002834704

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] イオントフォレシスによる高分子の皮膚透過2010

    • Author(s)
      小暮健太朗, 濱進, 氣賀澤郁, 梶本和昭
    • Journal Title

      生物物理 50(4)

      Pages: 188-189

    • NAID

      10026569471

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Noninvasive delivery of siRNA into the epidermis by iontophoresis using an atopic dermatitis-like model rat.2010

    • Author(s)
      Kigasawa K, Kajimoto K, Hama S, Saito A, Kanamura K, Kogure K.
    • Journal Title

      Int J Pharm. 383

      Pages: 157-160

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] イオントフォレシスによる高分子の皮膚透過2010

    • Author(s)
      小暮健太朗, 他2名、梶本和昭
    • Journal Title

      生物物理

      Volume: 50 Pages: 188-189

    • NAID

      10026569471

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Suppression of Tumor Growth by Transdermal Delivery of Oligonucleotides via Iontophoresis.2010

    • Author(s)
      Kogure K, Kigasawa K, Hama S, Kanamura K, Kajimoto K.
    • Organizer
      The 37th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Portland, USA
    • Year and Date
      2010-07-12
    • Related Report
      2010 Final Research Report
  • [Presentation] 網羅的遺伝子発現解析から分かる褐色脂肪細胞と白色脂肪細胞の分化と起源2010

    • Author(s)
      梶本和昭
    • Organizer
      日本薬学会北海道支部第134回例会 総説講演
    • Place of Presentation
      札幌
    • Year and Date
      2010-05-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 網羅的遺伝子発現解析に基づく脂肪細胞の多様性の解析2010

    • Author(s)
      梶本和昭, 白澤北斗, 小暮健太朗, 片岡正俊, 篠原康雄
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] 網羅的遺伝子発現解析に基づく脂肪細胞の多様性の解析2010

    • Author(s)
      梶本和昭
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      桃太郎アリーナ(岡山市)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] Noninvasive and effective transdermal delivery of siRNA by iontophoresis.2009

    • Author(s)
      Kogure K, Kigasawa K, Kanamura K, Kajimoto K.
    • Organizer
      Joint Symposium of the 5th Annual Meeting of the Oligonucleotide Therapeutics Society and the 19th Antisense Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2009-11-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Efficient and noninvasive transdermal delivery of siRNA by iontophoresis.2009

    • Author(s)
      Kogure K, Kigasawa K, Kanamura K, Kajimoto K.
    • Organizer
      The 36th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2009-07-22
    • Related Report
      2010 Final Research Report
  • [Presentation] イオントフォレシスによるナノ粒子化siRNA皮内送達システムの構築2009

    • Author(s)
      氣賀澤郁, 梶本和昭, 斎藤顕宜, 金村聖志, 小暮健太朗
    • Organizer
      日本薬学会129年会
    • Place of Presentation
      京都
    • Year and Date
      2009-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] ペプチドで肥満予防~経皮デリバリーの新たな可能性~2009

    • Author(s)
      梶本和昭
    • Organizer
      TIMS/MANA Joint Seminor
    • Place of Presentation
      筑波
    • Year and Date
      2009-01-29
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2018-03-28  

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