Attempt to the establishment of new approach for mouse germ cell induction in vivo.

• Project/Area Number: 20200071
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology; Obstetrics and gynecology
• Research Institution: Kumamoto University
• Principal Investigator: TANAKA Satomi 熊本大学, 発生医学研究所, 助教 (10321944)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥32,630,000 (Direct Cost: ¥25,100,000、Indirect Cost: ¥7,530,000); Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2009: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2008: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: 生殖細胞 / マウス / 応用動物科学 / 再生医療 / WNTシグナル / BMPシグナル / Dullard遺伝子 / 発生生物学 / Ifitm1遺伝子 / Ifitm3遺伝子 / 全胚培養系 / 生殖医学 / 微小環境

Research Abstract

To establish the new approach for germ cell induction in vivo, I examined the essential signals for germ cell formation in the mouse embryo. I found that in addition to Bmp signal, Wnt signal is also essential for germ cell formation, which is strictly regulated by Dullard. Further, I also found that Six1 and Six4 transcription factors act downstream to Bmp signal in mouse germ cell formation.

Research Products

• [Journal Article] Translocon- associated protein subunit Trap-gamma/ Ssr3 is required for vascular network formation in the mouse placenta. (2011)
  • Author(s): Yasuka L. Yamaguchi, *Satomi S. Tanaka, Naoko Oshima, Hiroshi Kiyonari, Makoto Asashima, and *Ryuichi Nishinakamura (*corresponding authors)
  • Journal Title: Dev Dyn Volume: 240 Pages: 394-403
  • Peer Reviewed
• [Journal Article] Impact of WNT signalling on tissue lineage differentiation in the early mouse embryo (2011)
  • Author(s): Satomi S.Tanaka
  • Journal Title: Development Growth Differentiation Volume: 53 Pages: 843-856
  • Peer Reviewed
• [Journal Article] Translocon-associated protein subunit Trap-Y/Ssr3 is required for vascular network formation in the mouse placenta (2011)
  • Author(s): Yasuka L Yamaguchi
  • Journal Title: Developmental Dynamics Volume: 240 Pages: 394-403
  • Peer Reviewed
• [Journal Article] Analyzing gene function in whole mouse embryo and fetal organ in vitro. (2011)
  • Author(s): Satomi S.Tanaka
  • Journal Title: Methods in Molecular Biology (Mark Lewandoski ed.) Volume: (In press)
• [Journal Article] Loss of Lhx1 Activity Impacts on the Localization of Primordial Germ Cells in the Mouse. (2010)
  • Author(s): *Satomi S. Tanaka, Yasuka L. Yamaguchi, Kirsten A. Steiner, Toru Nakano, Ryuichi Nishinakamura, Kin Ming Kwan, Richard R. Behringer, and Patrick P.L. Tam (*corresponding author)
  • Journal Title: Dev Dyn Volume: 239 Pages: 2851-2859
  • Peer Reviewed
• [Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme (2010)
  • Author(s): Yukako Uchiyama, Masagi Sakaguchi, Takeshi Terabayashi, Toshiaki Inenaga, Shuji Inoue, Chiyoko Kobayashi, Naoko Oshima, Hiroshi Kiyonari, Naoko Nakagata, Yuka Sato, Kiyotoshi Sekiguchi, Hiroaki Miki, Eiichi Araki, Sayoko Fujimura, Satomi S. Tanaka, Ryuichi Nishinakamura
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 9240-9245
  • Peer Reviewed
• [Journal Article] Loss of Lhx1 activity impacts on the localization of primordial germ cells in the mouse (2010)
  • Author(s): Satomi S.Tanaka
  • Journal Title: Developmental Dynamics Volume: 239(11) Pages: 2851-2859
  • Peer Reviewed
• [Journal Article] Kit26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme. (2010)
  • Author(s): Yukako Uchiyama
  • Journal Title: Proceedings of Natural Academy of Sciences of the United Sates of America Volume: 107 Pages: 9240-9245
  • Peer Reviewed
• [Journal Article] Draxin, a Repulsive Guidance Protein for Spinal Cord and Forebrain Commissures (2009)
  • Author(s): Shahidul M. Islam, Yohei Shinmyo, Tatsuya Okafuji, Yuhong Su, Iftekhar Bin Naser, Giasuddin Ahmed, Sanbing Zhang, Sandy Chen, Kunimasa Ohta, Hiroshi Kiyonari, Takaya Abe, Satomi Tanaka, Ryuichi Nishinakamura, Toshio Terashima, Toshio Kitamura, Hideaki Tanaka
  • Journal Title: Science Volume: 323 Pages: 388-393
  • Peer Reviewed
• [Journal Article] Draxin, a Repulsive Guidance Protein for Spinal Cord and Forebrain Commissures (2009)
  • Author(s): Shahidul M. Islam
  • Journal Title: Science 323 Pages: 388-393
  • Peer Reviewed
• [Journal Article] Analyzing gene function in whole mouse embryo and fetal organ in vitro
  • Author(s): Satomi S. Tanaka
  • Journal Title: Methods in Molecular Biology (Mark Lewandoski ed.) (In press)
• [Presentation] Dullard controls primordial germ cell formation by regulating canonical WNT signalling activity in the mouse embryo (2011)
  • Author(s): Satomi S.Tanaka
  • Organizer: Cold Spring harbor Asia conference, Developmental control of Sex, Growth and Cellular Fate
  • Place of Presentation: Suzhou, China
  • Year and Date: 2011-10-12
• [Presentation] Dullard controls primordial germ cell formation by regulating canonical WNT signalling in the mouse embryo (2010)
  • Author(s): S.S. Tanaka, et al.
  • Organizer: International Symposium on “Epigenome Network, Development and Reprogramming of Germ Cells”
  • Place of Presentation: Fukuoka, Japan. シーホークホテル
  • Year and Date: 2010-11-22
• [Presentation] Sall4 is a crucial transcriptional regulator for mouse primordial germ cell specification (2010)
  • Author(s): L. Y. Yamaguchi, S.S. Tanaka, et al.
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都, 国際会議場
  • Year and Date: 2010-05-22
• [Presentation] Dullard is required for mouse primordial germ cell formation by coordinating canonical Wnt signalling in the embryo (2010)
  • Author(s): S.S. Tanaka, et al.
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都, 国際会議場
  • Year and Date: 2010-05-21
• [Presentation] Dullard is required for mouse primordial germ cell formation by coordinating canonical Wnt signalling in the embryo (2009)
  • Author(s): S.S. Tanaka, et al.
  • Organizer: 日本発生生物学会
  • Place of Presentation: 新潟, 朱鷺メッセ
  • Year and Date: 2009-05-30
• [Presentation] Dullard is required for mouse primordial germ cell formation by coordinating canonical Wnt signalling in the embryo. (2009)
  • Author(s): Satomi S.Tanaka
  • Organizer: 42^<th> Annual meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 新潟、朱鷺メッセ
  • Year and Date: 2009-05-30
• [Presentation] Six1 and Six4 Homeoproteins are required for Sex Determination in Mice Gonad (2009)
  • Author(s): Y. Fujimoto, S.S. Tanaka, et al.
  • Organizer: 日本発生生物学会
  • Place of Presentation: 新潟, 朱鷺メッセ
  • Year and Date: 2009-05-28
• [Presentation] Six1 and Six4 Homeoproteins are required for Sex Determination in Mice Gonad. (2009)
  • Author(s): Yuka Fujimoto
  • Organizer: 42^<th> Annual meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 新潟、朱鷺メッセ
  • Year and Date: 2009-05-28
• [Presentation] Functional analysis of Dullard in ES cell differentiation, which is essential for germ cell formation in the gastrulating mouse embryo (2008)
  • Author(s): S.S. Tanaka, et al.
  • Organizer: The 23^<rd> Naito Conference
  • Place of Presentation: 神奈川, 湘南国際村
  • Year and Date: 2008-11-12
• [Presentation] Functional analysis of Dullard in ES cell differentiation, which is essential for germ cell formation in the gastrulating mouse embryo (2008)
  • Author(s): Satomi S. Tanaka
  • Organizer: The 23^<rd> Naito Conference
  • Place of Presentation: 神奈川
  • Year and Date: 2008-11-12
• [Presentation] Dullard is essential for primordial germ cell formation and posterior extraembryonic mesoderm differentiation in the gastrulating mouse embryo (2008)
  • Author(s): S.S. Tanaka, et al.
  • Organizer: 日本発生生物学会
  • Place of Presentation: 徳島, 徳島文化会館
  • Year and Date: 2008-05-29
• [Presentation] Dullard is essential for primordial germ cell formation and posterior extr aembryonic mesoderm differentiation in the gastrulating mouse embryo (2008)
  • Author(s): Satomi S. Tanaka
  • Organizer: 日本発生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-29
• [Presentation] Ifitm/mil/fragilis ファミリーによるマウス生殖細胞の移動の制御 (2008)
  • Author(s): 田中聡, 他
  • Organizer: 頭部形成研究会
  • Place of Presentation: 熊本, 阿蘇, ホテルグリーンピア南阿蘇
  • Year and Date: 2008-04-15
• [Book] Analyzing gene function in whole mouse embryo and fetal organ in vitro. In Methods in Molecular Biology (Mark Lewandoski ed.), ISBN: 978-1-60327-290-2 (2012)
  • Author(s): Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam.
  • Publisher: Totowa NJ: Humana press(in press)
• [Book] Methods in Molecular Biology ISBN 978-1-60327-290-2(Mark Lewandoski ed.) (2012)
  • Author(s): Satomi S.Tanaka
  • Publisher: Analyzing gene function in whole mouse embryo and fetal organ in vitro(In press)