Characterization and applications of enzymatic cross-linking reactions
| Project/Area Number |
20200072
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
KIYOTAKA Hitomi 名古屋大学, 生命農学研究科, 准教授 (00202276)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMIYA Noriho 九州大学, 大学院・工学系研究科, 教授 (50302766)
MTSUFUJI Senya 東京慈恵会医科大学, 医学部, 教授 (50192753)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥33,800,000 (Direct Cost: ¥26,000,000、Indirect Cost: ¥7,800,000)
Fiscal Year 2010: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2008: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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| Keywords | 酵素 / 基質 / カルシウム / 蛋白質接着 / 基質配列 / トランスグルタミナーゼ / タンパク質架橋 / 翻訳後修飾 / 表皮 |
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| Research Abstract |
Enzymatic cross-linking is essential reaction for several biological events: blood coagulation, skin formation. Transglutaminase, which is responsible for the reaction, is a family of enzyme consisting of 8 isozymes. In this project, I have carried out the identification of highly reactive substrate peptide which is useful tool for resolving of physiological significance. Furthermore, application of the peptide has been attempted.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles2011
Author(s)
Yamane, A., Fukui, M., Sugimura, Y., Itoh, M., Alea, M. P, Said, El Alaoui, Akiyama, M., Hitomi, K.
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Journal Title
FEBS J.
Volume: 277
Pages: 3564-3574
Related Report
Peer Reviewed
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[Journal Article] A Specific Colorimetric assay for measuring of transglutaminase 1 and factor XIII activities2009
Author(s)
Hitomi, K., Kitamura, M., Alea, M. P., Ismail, C., Thomas, V., Alaoui, S El.
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Journal Title
Anal. Biochem.
Volume: 394
Pages: 281-283
Related Report
Peer Reviewed
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[Journal Article] Development of an isozyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking assay2009
Author(s)
Alea, M. P., Kitamura, M., Martin, G., Thomas, V., Hitomi, K., Alaoui, S El.
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Journal Title
Anal. Biochem.
Volume: 389
Pages: 150-156
Related Report
Peer Reviewed
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[Journal Article] Development of an isozyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking assay.2009
Author(s)
Alea, M.P., Kitamura, M., Martin, G., Thomas, V., Hitomi, K., Alaoui, S El.
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Journal Title
Analytical Biochemistry 389
Pages: 150-156
Related Report
Peer Reviewed
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[Presentation]2009
Author(s)
Kiyotaka HITOMI
Organizer
ゴードン国際会議「ポリアミン」
Place of Presentation
アメリカニューハンプシャー州
Related Report
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