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Identification of neurons by fluorescence spectrogram through a patch electrode, and the application to auditory neural circuits in vivo

Research Project

Project/Area Number 20220008
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKyoto University

Principal Investigator

OHMORI Harunori  京都大学, 医学研究科, 教授 (30126015)

Project Period (FY) 2008 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥165,230,000 (Direct Cost: ¥127,100,000、Indirect Cost: ¥38,130,000)
Fiscal Year 2012: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2011: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2010: ¥38,740,000 (Direct Cost: ¥29,800,000、Indirect Cost: ¥8,940,000)
Fiscal Year 2009: ¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2008: ¥37,830,000 (Direct Cost: ¥29,100,000、Indirect Cost: ¥8,730,000)
Keywordsパッチ電極 / 神経細胞 / 蛍光蛋白 / 神経回路 / 聴覚 / 蛍光トレーサー / 蛍光電極 / パッチクランプ / 分光器 / 聴覚神経回路 / オレゴングリーンBAPTA / Field-L / FRET / 蛍光分光 / 蛍光タンパク
Research Abstract

This study aimed at recording optical signal simultaneously with neuronal electrical activity in the deep brain tissue. For that purpose, we developed a photometric patch electrode (PME) recording system by using a patch electrode as a light guide. By filling the neuron in the auditory nuclei of a chick with Oregon green BAPTA-1-AM (OGB-1), intracellular Ca2+signals were recorded simultaneously with electrical signal in response to acoustic stimulus in the Field-L, the avian auditory cortex, and in the inferior colliculus. A slow field current was enhanced after the block of GABA receptor in the Field-L, which enhanced the Ca2+wave that was generated in synchrony with the electrical slow wave. In the inferior colliculus, Ca2+responses were similarly recorded and were enhanced after the block of GABA receptor. Therefore, we succeeded in development of PME recording system to monitor neuronal electrical activity simultaneously with optical signal through the patch electrode in the deep brain tissue.

Assessment Rating
Verification Result (Rating)

A

Report

(6 results)
  • 2013 Research Progress Assessment (Verification Result) ( PDF )
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • Research Products

    (46 results)

All 2013 2012 2011 2010 2009 2008 Other

All Journal Article (26 results) (of which Peer Reviewed: 25 results) Presentation (12 results) (of which Invited: 1 results) Book (2 results) Remarks (6 results)

  • [Journal Article] Activation of metabotropic glutamate receptors improves the accuracy of coincidence detection by presynaptic mechanisms in the nucleus laminaris of the chick.2013

    • Author(s)
      Okuda H, Yamada R, Kuba H & Ohmori H
    • Journal Title

      Journal of Physiology

      Volume: 591.1 Issue: 1 Pages: 365-378

    • DOI

      10.1113/jphysiol.2012.244350

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hyperpolarisationactivated cyclic nucleotide-gated channels regulate the spontaneous firing rate of olfactory receptor neurons and affect glomerular formation in mice.2013

    • Author(s)
      Nakashima N, Ishii TM, Bessho Y, Kageyama R & Ohmori H
    • Journal Title

      J Physiology

      Volume: 591 Issue: 7 Pages: 1749-1769

    • DOI

      10.1113/jphysiol.2012.247361

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The cooperation of sustained and phasic inhibitions increases the contrast of ITD-tuning in low-frequency neurons of the chick nucleus laminaris.2013

    • Author(s)
      Yamada R, Okuda H, Kuba H, Nishino E, Ishii TM & Ohmori H
    • Journal Title

      J Neuroscience

      Volume: 33(9) Issue: 9 Pages: 3927-38

    • DOI

      10.1523/jneurosci.2377-12.2013

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinct neural firing mechanisms to tonal stimuli offset in the inferior colliculus of mice in vivo.2012

    • Author(s)
      Kasai M, Ono M, Ohmori H.
    • Journal Title

      Neurosci Res.

      Volume: 73(3) Issue: 3 Pages: 224-37

    • DOI

      10.1016/j.neures.2012.04.009

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of presynaptic Na+/K+ ATPase reduces readily releasable pool size at the avian end-bulb of Held synapse.2012

    • Author(s)
      Taruno A
    • Journal Title

      Neuroscience Research

      Volume: 72 Issue: 2 Pages: 117-128

    • DOI

      10.1016/j.neures.2011.11.003

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of presynaptic Na+/K+ ATPase reduces readily releasable pool size at the avian end-bulb of Held synapse2012

    • Author(s)
      Taruno, A, Ohmori H, Kuba H
    • Journal Title

      Neuroscience Research

      Volume: 29 Pages: 117-128

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition in the balance: binaurally coupled inhibitory feedback in sound localization circuitry.2011

    • Author(s)
      Burger, R.M., Fukui, I., Ohmori, H., and Rubel, E.W.
    • Journal Title

      Journal of Neurophysiology

      Volume: 106 Issue: 1 Pages: 4-14

    • DOI

      10.1152/jn.00205.2011

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition in the balance : binauraliy coupled inhibitory feedback in sound localization circuitry2011

    • Author(s)
      Burger, R.M., Fukui, I., Ohmori, H., Rubel, E.W.
    • Journal Title

      Journal of Neurophysiology

      Volume: 106 Pages: 4-14

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 音源定位の神経回路機構2011

    • Author(s)
      福井巌、大森治紀
    • Journal Title

      Clinical Neuroscience

      Volume: 29(12) Pages: 1388-1391

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Interaural phase difference modulates the neural activity in the nucleus angularis and improves the processing of level difference cue in the lateral lemniscal nucleus in the chicken2010

    • Author(s)
      Sato T, Fukui I and Ohmori H.
    • Journal Title

      NeuroscienceResearch

      Volume: 66 Issue: 2 Pages: 198-212

    • DOI

      10.1016/j.neures.2009.11.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] GABAergic inhibition sharpens the frequency tuning and enhances phase locking in chicken nucleus magnocellularis neurons.2010

    • Author(s)
      Iwao Fukui, R. Michael Burger, Harunori Ohmori, and Edwin Rubel.
    • Journal Title

      J Neuroscience

      Volume: 30(36) Issue: 36 Pages: 12075-12083

    • DOI

      10.1523/jneurosci.1484-10.2010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Presynpatic activity regulates Na+ channel distribution at the axon intial segment.2010

    • Author(s)
      Kuba H, Oichi Y & Ohmori H.
    • Journal Title

      Nature

      Volume: 465 Issue: 7301 Pages: 1075-1078

    • DOI

      10.1038/nature09087

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaural phase difference modulates the neural activity in the nucleus angularis and improves the processing of level difference cue in the lateral lemniscal nucleus in the chicken.2010

    • Author(s)
      Sato T, Fukui I, Ohmori H.
    • Journal Title

      Neuroscience Research 66

      Pages: 198-212

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] GABAergic inhibition sharpens the frequency tuning and enhances phase locking in chicken nucleus magnocellularis neurons.2010

    • Author(s)
      Iwao Fukui, R. Michael Burger, Harunori Ohmori, Edwin Rubel.
    • Journal Title

      J Neuroscience 30(36)

      Pages: 12075-12083

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Presynpatic activity regulates Na+ channel distribution at the axon intial segment.2010

    • Author(s)
      Kuba H, Oichi Y, Ohmori H.
    • Journal Title

      Nature 465

      Pages: 1075-1078

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Interaural phase difference modulates the neural activity in the nucleus angularis and improves the processing of level difference cue in the lateral lemniscal nucleus in the chicken2010

    • Author(s)
      Sato T, Fukui I, Ohmori H
    • Journal Title

      Neuroscience Research

      Volume: 66 Pages: 198-212

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Presynpatic activity regulates Na+ channel distribution at the axon intial segment2010

    • Author(s)
      Kuba H, Oichi Y, Ohmori H
    • Journal Title

      Nature

      Volume: 465 Pages: 1075-1078

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GABAergic inhibition sharpens the frequency tuning and enhances phase locking in chicken nucleus magnocellularis neurons2010

    • Author(s)
      Iwao Fukui, R.Michael Burger, Harunori Ohmori, Edwin Rubel
    • Journal Title

      J Neuroscience

      Volume: 30 Pages: 12075-12083

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of axonal sodium channels in precise auditory time coding at nucleus magnocellularis of the chick.2009

    • Author(s)
      Kuba,H. and Ohmori,H.
    • Journal Title

      Journal of Physiology

      Volume: 587(Pt1) Issue: 1 Pages: 87-100

    • DOI

      10.1113/jphysiol.2008.162651

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The modulation by intensity of the processing of interaural timing cues for localizing sounds.2009

    • Author(s)
      Eri Nishino and H Ohmori.
    • Journal Title

      Molecular Neurobiology

      Volume: 40(2) Issue: 2 Pages: 157-65

    • DOI

      10.1007/s12035-009-8078-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of axonal sodium channels in precise auditory time coding at nucleus magnocellularis of the chick.2009

    • Author(s)
      Kuba, H., Ohmori,H.
    • Journal Title

      Journal of Physiology 2009 587(Pt1)

      Pages: 87-100

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The modulation by intensity of the processing of interaural timing cues for localizing sounds.2009

    • Author(s)
      Eri Nishino, H Ohmori.
    • Journal Title

      Molecular Neurobiology 40(2)

      Pages: 157-165

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Presynaptic GABAB receptors modulatesynaptic facilitation and depression atdistinct synapses in fusiform cells ofmouse dorsal cochlear nucleus.2008

    • Author(s)
      Tomohiko Irie and Harunori Ohmori
    • Journal Title

      BBRC

      Volume: 367 Issue: 2 Pages: 503-508

    • DOI

      10.1016/j.bbrc.2008.01.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sound-Intensity-Dependent Compensation for the Small Interaural Time Difference Cue for Sound Source Localization.2008

    • Author(s)
      Eri Nishino, R.Yamada, H.Kuba, H.Hioki, T.Furuta, T.Kaneko, and H.Ohmori.
    • Journal Title

      Journal of Neuroscience

      Volume: 28(28) Issue: 28 Pages: 7053-7164

    • DOI

      10.1523/jneurosci.4398-07.2008

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Presynaptic GABAB receptors modulate synaptic facilitation and depression at distinct synapses in fusiform cells of mouse dorsal cochlear nucleus.2008

    • Author(s)
      Tomohiko Irie, Harunori Ohmori.
    • Journal Title

      BBRC 3672008

      Pages: 503-508

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Sound-Intensity-Dependent Compensation for the Small Interaural Time Difference Cue for Sound Source Localization.2008

    • Author(s)
      Eri Nishino, R. Yamada, H. Kuba, H. Hioki, T. Furuta, T. Kaneko, H. Ohmori
    • Journal Title

      Journal of Neuroscience 28(28)

      Pages: 7153-7164

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] トリ聴覚同時検出器細胞の樹状突起フィルター特性2013

    • Author(s)
      山田 玲
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] HCNチャネルは、嗅細胞の自発発火頻度を制御し、嗅丘への軸索投射に影響を与える2013

    • Author(s)
      中島則行
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 蛍光パッチ電極法による電気記録と蛍光信号の同時計測2013

    • Author(s)
      西野恵里
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] トリ蝸牛神経核大細胞核ニューロンにおけるシナプス入力の統合と活動電位発射メカニズムのin vivo解析2013

    • Author(s)
      平井康治
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 時間差の検出と計算機シミュレーション2013

    • Author(s)
      福井 巌
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 蛍光分光及び電気活動の同時計測を目指した蛍光パッチ電極法の開発2012

    • Author(s)
      西野恵里、大森治紀
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      長野県松本文化会館
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Tonotopic specializations in the timing coding pathway in the avian auditory nuclei; NM and NL.2012

    • Author(s)
      Ohmori H
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Auditory System, Bates College, Lewiston, ME USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Tonotopic specialization in the timing coding pathway in the avian auditory nuclei; NM and NL.2012

    • Author(s)
      Ohmori, H
    • Organizer
      Gordon Research Conference, Auditory system.
    • Place of Presentation
      Bates College, ME, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Two modes of inhibition enhances the contrast of ITD tuning in the chick.2011

    • Author(s)
      Ohmori H
    • Organizer
      7th FAOPS,Special Lecture
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2011-11-14
    • Related Report
      2012 Final Research Report
  • [Presentation] in vivo whole cell記録を用いたマウス下丘offsetニューロンの発火機構解析2010

    • Author(s)
      笠井昌俊、小野宗範、大森治紀
    • Organizer
      第87回日本生理学会
    • Place of Presentation
      盛岡市民文化ホール
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sound intensity dependent compensation for the small ITD cue in the chicken.2009

    • Author(s)
      Ohmori H
    • Organizer
      第36回 国際生理学会
    • Place of Presentation
      京都
    • Year and Date
      2009-07-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Sound intensity dependent compensation for the small ITD cue in the chicken.2009

    • Author(s)
      Ohmori H.
    • Organizer
      IUPS Congress 2009
    • Place of Presentation
      Kyoto
    • Year and Date
      2009-07-29
    • Related Report
      2010 Self-evaluation Report
  • [Book] 標準生理学 第7版2009

    • Author(s)
      大森治紀
    • Publisher
      医学書院
    • Related Report
      2012 Final Research Report
  • [Book] 標準生理学第7版2009

    • Publisher
      医学書院
    • Related Report
      2010 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.nbiol.med.kyoto-u.ac.jp

    • Related Report
      2012 Final Research Report
  • [Remarks] 京都大学大学院医学研究科・医学部

    • URL

      http://www.med.kyoto-u.ac.jp/organization-staff/research/doctoral_course/r-019/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 神経生物学教室

    • URL

      http://www.nbiol.med.kyoto-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • Related Report
      2011 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1603/

    • Related Report
      2010 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/1603/

    • Related Report
      2010 Annual Research Report

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Published: 2008-04-01   Modified: 2019-07-29  

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