|Budget Amount *help
¥165,230,000 (Direct Cost: ¥127,100,000、Indirect Cost: ¥38,130,000)
Fiscal Year 2012: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2011: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2010: ¥38,740,000 (Direct Cost: ¥29,800,000、Indirect Cost: ¥8,940,000)
Fiscal Year 2009: ¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2008: ¥37,830,000 (Direct Cost: ¥29,100,000、Indirect Cost: ¥8,730,000)
This study aimed at recording optical signal simultaneously with neuronal electrical activity in the deep brain tissue. For that purpose, we developed a photometric patch electrode (PME) recording system by using a patch electrode as a light guide. By filling the neuron in the auditory nuclei of a chick with Oregon green BAPTA-1-AM (OGB-1), intracellular Ca2+signals were recorded simultaneously with electrical signal in response to acoustic stimulus in the Field-L, the avian auditory cortex, and in the inferior colliculus. A slow field current was enhanced after the block of GABA receptor in the Field-L, which enhanced the Ca2+wave that was generated in synchrony with the electrical slow wave. In the inferior colliculus, Ca2+responses were similarly recorded and were enhanced after the block of GABA receptor. Therefore, we succeeded in development of PME recording system to monitor neuronal electrical activity simultaneously with optical signal through the patch electrode in the deep brain tissue.