| Budget Amount *help |
¥168,220,000 (Direct Cost: ¥129,400,000、Indirect Cost: ¥38,820,000)
Fiscal Year 2012: ¥27,040,000 (Direct Cost: ¥20,800,000、Indirect Cost: ¥6,240,000)
Fiscal Year 2011: ¥27,040,000 (Direct Cost: ¥20,800,000、Indirect Cost: ¥6,240,000)
Fiscal Year 2010: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
Fiscal Year 2009: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2008: ¥38,220,000 (Direct Cost: ¥29,400,000、Indirect Cost: ¥8,820,000)
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| Research Abstract |
A cationized gelatin of gene transfection was prepared by chemical introducing spermine to gelatin. Gelatin sponge scaffolds mechanically reinforced by ceramic granules incorporation were combined with growth factors and a complex of ca tionized gelatin and plasmid DNA. Bone marrow derived mesenchymal stem cells (MSC) were cultured for gene transfection in the sponge prepared. Comparing with the conventional gene transfection, a higher efficiency of MSC genetically engineering was observed. Upone combining with a bioreactor of culture medium circulating system, the engineering efficiency was further improved. This culture technology is applicable for small interfering DNA, and demonstrated to be a promising engineering technology of cell bioactivity.
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