Fathoming the evolution of gene regulation through an 'arms race' between transposons and Argonautes

• Project/Area Number: 20221008
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: Keio University
• Principal Investigator: SIOMI Haruhiko 慶應義塾大学, 医学部, 教授 (60202107)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥213,330,000 (Direct Cost: ¥164,100,000、Indirect Cost: ¥49,230,000); Fiscal Year 2012: ¥31,200,000 (Direct Cost: ¥24,000,000、Indirect Cost: ¥7,200,000); Fiscal Year 2011: ¥31,200,000 (Direct Cost: ¥24,000,000、Indirect Cost: ¥7,200,000); Fiscal Year 2010: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000); Fiscal Year 2009: ¥52,000,000 (Direct Cost: ¥40,000,000、Indirect Cost: ¥12,000,000); Fiscal Year 2008: ¥57,330,000 (Direct Cost: ¥44,100,000、Indirect Cost: ¥13,230,000)
• Keywords: ゲノム機能 / RNAサイレンシング / 転移因子 / 小分子RNA / RNAi / ゲノム進化 / エピゲネティクス / 生殖細胞 / 生殖幹細胞 / Argonaute / piRNA / ゲノム / 進化 / Piwi / Yb Body / エピジェネティックス / エピジェネティクス / 転位因子 / RNA サイレンシング / 脆弱X症候群

Research Abstract

To test the hypothesis that the arms race between transposable elements (TEs) and hosts leads to positive selection for cellular defense mechanisms, parts of which are co-opted for evolving new regulatory circuits, we have elucidated RNA silencing pathway

Assessment Rating

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Research Products

• [Journal Article] Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila. (2013)
  • Author(s): Nishida, KM.
  • Journal Title: Molecular Cell Volume: 49 Issue: 4 Pages: 680-691
  • DOI: 10.1016/j.molcel.2012.12.024
  • Peer Reviewed
• [Journal Article] Structure andfunction of Zucchini endoribonuclease in piRNA biogenesis. (2012)
  • Author(s): Nishimasu, H., Ishizu, H., Saito, K., Fukuhara, S., Kamatani, MK., Matsumoto, N., Nishizawa, T., Bonnefond, L., Nakanaga, K., Aoki, J., Ishitani, R., Siomi, H., Siomi, MC., and Nureki, O
  • Journal Title: Nature Volume: 491 Pages: 284-287
  • Peer Reviewed
• [Journal Article] Structure and function of Zucchini endoribonuclease in piRNA biogenesis. (2012)
  • Author(s): Hiroshi Nishimasu, Hirotsugu Ishizu
  • Journal Title: Nature Volume: 491 Issue: 7423 Pages: 284-287
  • DOI: 10.1038/nature11509
  • Peer Reviewed
• [Journal Article] Biology of PlWI-interacting RNAs : new insights into biogenesis and function inside and outside of germlines. (2012)
  • Author(s): Hirotsugu Ishizu
  • Journal Title: Genes and Development Volume: 26 Issue: 21 Pages: 2361-2373
  • DOI: 10.1101/gad.203786.112
  • Peer Reviewed
• [Journal Article] Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC. (2011)
  • Author(s): Sato, K., Nishida, K.M., Shibuya, A., Siomi, M.C., and Siomi, H
  • Journal Title: Genes & Development Volume: 25 Pages: 2361-2371
  • Peer Reviewed
• [Journal Article] Chromatin-associated RNAi components contribute to transcriptional regulation in Drosophila. (2011)
  • Author(s): Cernilogar, F.M., Onorati, M.C., Kothe, G.O., Burroughs, A.M., Parsi, K.M., Breiling, A., lo Sardo, F., Saxena, A., Miyoshi, K., Siomi, H., Siomi, M.C., Carninci, P., Gilmour, D.S., Corona, D.F.V., and Orlando, V.
  • Journal Title: Nature Volume: 480 Pages: 391-395
  • Peer Reviewed
• [Journal Article] Biochemical analyses of endogenous Argonaute complexes immunopurified with anti-Argonaute monoclonal antibodies (2011)
  • Author(s): Miyoshi, K.
  • Journal Title: Methods in Molecular Biology Volume: 725 Pages: 29-43
  • DOI: 10.1007/978-1-61779-046-1_3
  • ISBN: 9781617790454, 9781617790461
  • Peer Reviewed
• [Journal Article] Gatekeepers for Piwi-piRNA complexes to enter the nucleus (2011)
  • Author(s): Hirotsugu Ishizu
  • Journal Title: Current Opinion in Genetics & Development Volume: 21 Issue: 4 Pages: 484-490
  • DOI: 10.1016/j.gde.2011.05.001
  • Peer Reviewed
• [Journal Article] Stress signaling etches heritable marks on chromatin (2011)
  • Author(s): Siomi, H.
  • Journal Title: Cell Volume: 145 Issue: 7 Pages: 1005-1007
  • DOI: 10.1016/j.cell.2011.06.009
  • Peer Reviewed
• [Journal Article] Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila (2011)
  • Author(s): Nagao, A.
  • Journal Title: Front.Gene. Volume: 2 Pages: 55-55
  • DOI: 10.3389/fgene.2011.00055
  • Peer Reviewed
• [Journal Article] Chromatin-associated RNAi components contribute to transcriptional regulation in Drosophila (2011)
  • Author(s): Cernilogar, F.M.
  • Journal Title: Nature Volume: 480 Issue: 7377 Pages: 391-395
  • DOI: 10.1038/nature10492
  • Peer Reviewed
• [Journal Article] Maelstrom coordinates microtubule organization during Drosophilaoogenesis through interaction with components of the MTOC (2011)
  • Author(s): Sato, K.
  • Journal Title: Genes & Development Volume: 25 Issue: 22 Pages: 2361-2373
  • DOI: 10.1101/gad.174110.111
  • Peer Reviewed
• [Journal Article] Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila (2010)
  • Author(s): Saito, K., Ishizu, H., Komai, M., Kotani, H., Kawamura, Y., Nishida, KM., Siomi, H., and Siomi, MC.
  • Journal Title: Genes & Development Volume: 24 Pages: 2493-2498
  • Peer Reviewed
• [Journal Article] Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila. (2010)
  • Author(s): Nagao, A., Mituyama, T., Huang, H., Chen, D., Siomi, MC., Siomi, H
  • Journal Title: RNA Volume: 16 Pages: 2503-2515
  • Peer Reviewed
• [Journal Article] A direct role of Hsp90 in pre-RISC formation in Drosophila (2010)
  • Author(s): Miyoshi, T., Takeuchi, A., Siomi, H. and Siomi, M.C.
  • Journal Title: Nature Structural and Molecular Biology Volume: 17 Pages: 1024-1026
  • Peer Reviewed
• [Journal Article] Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. (2010)
  • Author(s): Saito, K., Ishizu, H., Komai, M., Kotani, H., Kawamura, Y., Nishida, KM., Siomi, H., Siomi, MC.
  • Journal Title: Genes, Development 24 Pages: 2493-2498
• [Journal Article] Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila. (2010)
  • Author(s): Nagao, A., Mituyama, T., Huang, H., Chen, D., Siomi, MC., Siomi, H.
  • Journal Title: RNA 16 Pages: 2503-2515
• [Journal Article] A direct role of Hsp90 in pre-RISC formation in Drosophila (2010)
  • Author(s): Miyoshi, T., Takeuchi, A., Siomi, H., Siomi, M.C.
  • Journal Title: Nature Structural and Molecular Biology 17 Pages: 1024-1026
• [Journal Article] How does the royal family of Tudor rule the Piwi-interacting RNA pathway? (2010)
  • Author(s): Siomi, M.C., Mannen, T., Siomi, H.
  • Journal Title: Genes & Development 24 Pages: 636-646
• [Journal Article] piRNA-mediated silencing in Drosophila germlines. (2010)
  • Author(s): Siomi, MC.
  • Journal Title: Seminars in Cell & Developmental Biology Volume: 21 Pages: 754-759
  • Peer Reviewed
• [Journal Article] How does the royal family of Tudor rule the Piwi-interacting RNA pathway? (2010)
  • Author(s): Siomi, MC.
  • Journal Title: Genes & Development Volume: 24 Pages: 636-646
  • Peer Reviewed
• [Journal Article] Posttranscriptional regulation of miRNA biogenesis in animals. (2010)
  • Author(s): Siomi, H.
  • Journal Title: Molecular Cell Volume: 38 Pages: 323-332
  • Peer Reviewed
• [Journal Article] Biochemical analyses of endogenous Argonaute complexes immunopurified with anti-Argonaute monoclonal antibodies. (2010)
  • Author(s): Miyoshi, K.
  • Journal Title: Methods in Molecular Biology Volume: (in press)
  • Peer Reviewed
• [Journal Article] A direct role of Hsp90 in pre-RISC formation in Drosophila. (2010)
  • Author(s): Miyoshi, T.
  • Journal Title: Nature Structural and Molecular Biology Volume: 17 Pages: 1024-1026
  • Peer Reviewed
• [Journal Article] Many ways to generate microRNA-like small RNAs : noncanonical pathways for microRNA production. (2010)
  • Author(s): Miyoshi, K.
  • Journal Title: Mol.Genet, Genomics Volume: 284 Pages: 95-103
  • Peer Reviewed
• [Journal Article] The key features of RNA silencing. (2010)
  • Author(s): Saito, K.
  • Journal Title: In RNA Technologies and Their Applications Volume: 1-28
  • Peer Reviewed
• [Journal Article] Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. (2010)
  • Author(s): Saito, K.
  • Journal Title: Genes & Development Volume: 24 Pages: 2493-2498
  • Peer Reviewed
• [Journal Article] Natural variation of the amino-terminal glutamine-rich domain in Drosophila Argonaute2 is not associated with developmental defects. (2010)
  • Author(s): Hain, D.
  • Journal Title: PLoS One Volume: 5(12)
  • Peer Reviewed
• [Journal Article] Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila. (2010)
  • Author(s): Keita Miyoshi
  • Journal Title: RNA 16 Pages: 506-515
  • Peer Reviewed
• [Journal Article] piRNA-mediated silencing in Drosophila germlines. (2010)
  • Author(s): Siomi, MC.
  • Journal Title: Seminars in Cell & Developmental Biology (In press)
  • Peer Reviewed
• [Journal Article] How does the royal family of Tudor rule the Piwi-interacting RNA pathway? (2010)
  • Author(s): Siomi, M.C.
  • Journal Title: Genes & Development 24 Pages: 636-646
  • Peer Reviewed
• [Journal Article] Is canalization more than a beautiful idea? (2010)
  • Author(s): Sato, K.
  • Journal Title: Genome Biology 11 Pages: 109-112
• [Journal Article] Posttranscriptional regulation of miRNA biogenesis in animals. (2010)
  • Author(s): Haruhiko Siomi
  • Journal Title: Molecular Cell (In press)
  • Peer Reviewed
• [Journal Article] On the road to reading the RNA interference code (2009)
  • Author(s): Siomi, H., and Siomi, MC.
  • Journal Title: Nature Volume: 457 Pages: 396-404
  • Peer Reviewed
• [Journal Article] A regulatory circuit for piwi by traffic jam, a large Maf, in Drosophila gonadal somas (2009)
  • Author(s): Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi H. and Siomi, MC
  • Journal Title: Nature Volume: 461 Pages: 1296-1299
  • Peer Reviewed
• [Journal Article] Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines. (2009)
  • Author(s): Nishida, KM., Okada, TN., Kawamura, T., Mituyama, T., Kawamura, Y., Inagaki, S., Huang, H., Chen, D., Kodama, T., Siomi, H., Siomi, MC.
  • Journal Title: EMBO J. 28 Pages: 3820-3831
• [Journal Article] A regulatory circuit for piwi by traffic jam, a large Maf, in Drosophila gonadal somas (2009)
  • Author(s): Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi H., Siomi, MC.
  • Journal Title: Nature 461 Pages: 1296-1299
• [Journal Article] RISC hitches onto endosome trafficking. (2009)
  • Author(s): Siomi, H., Siomi, MC.
  • Journal Title: Nature Cell Biology 11 Pages: 1049-1051
• [Journal Article] On the road to reading the RNA interference code (2009)
  • Author(s): Siomi, H., Siomi, MC.
  • Journal Title: Nature 457 Pages: 396-404
• [Journal Article] Characterization of miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway. (2009)
  • Author(s): Keita Miyoshi
  • Journal Title: RNA 15 Pages: 1282-1291
  • Peer Reviewed
• [Journal Article] RISC hitches onto endosome trafficking. (2009)
  • Author(s): Haruhiko Siomi
  • Journal Title: Nature Cell Biology 11 Pages: 1049-1051
• [Journal Article] A regulatory circuit for piwi by traffic jam, a large Maf, in Drosophila gonadal somas. (2009)
  • Author(s): Kuniaki Saito
  • Journal Title: Nature 461 Pages: 1296-1299
  • Peer Reviewed
• [Journal Article] A novel microRNA regulatory mechanism of osteoblast differentiation. (2009)
  • Author(s): Hiroyuki Inose
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 20794-20799
  • Peer Reviewed
• [Journal Article] Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines. (2009)
  • Author(s): Nishida, KM
  • Journal Title: EMBO J. 28 Pages: 3820-3831
  • Peer Reviewed
• [Journal Article] On the road to reading the RNA interference code. (2009)
  • Author(s): Siomi, H., et al.
  • Journal Title: Nature 457 Pages: 396-404
  • Peer Reviewed
• [Journal Article] Fragile X carrier screening and FMRl allele distribution in the Japanese population. (2009)
  • Author(s): Otsuka, S., et al.
  • Journal Title: Brain Dev. (in press)
  • Peer Reviewed
• [Journal Article] Overexpression of HMGA2 relates to reduction of the let-7 and its relationship to clinicopathological features in pituitary adenomas. (2009)
  • Author(s): Qian ZR, et al.
  • Journal Title: Mod Pathol. 2 Pages: 431-441
  • Peer Reviewed
• [Journal Article] Drosophilaendogenous small RNAs bind to Argonaute2 in somatic cells (2008)
  • Author(s): Kawamura, Y., Saito, K., Kin, T., Ono, Y., Asai, K., Sunohara, T., Okada, NT., Siomi MC. and Siomi, H.
  • Journal Title: Nature Volume: 453 Pages: 793-797
  • Peer Reviewed
• [Journal Article] Drosophila endogenous small RNAs bind to Argonaute2 in somatic cells. (2008)
  • Author(s): Kawamura, Y., Saito, K., Kin, T., Ono, Y., Asai, K., Sunohara, T., Okada, NT., Siomi MC., Siomi, H.
  • Journal Title: Nature 453 Pages: 793-797
• [Journal Article] Characterization of endogenous human Argonautes and their miRNA partners in RNA silencing. (2008)
  • Author(s): Azuma-Mukai, A., et al.
  • Journal Title: Proc. Natl. Acad. Sci. 105 Pages: 7964-7969
  • Peer Reviewed
• [Journal Article] Drosophila endogenous small RNAs bind to Argonaute2 in somatic cells. (2008)
  • Author(s): Kawamura, Y., et al.
  • Journal Title: Nature 453 Pages: 793-797
  • Peer Reviewed
• [Journal Article] Circadian phenotypes of Drosophila fragile X mutants in alternative genetic backgrounds. (2008)
  • Author(s): Sekine, T., et al.
  • Journal Title: Zoological Science 25 Pages: 561-571
  • Peer Reviewed
• [Journal Article] Interactions between transposable elements and Argonautes have (probably) been shaping the Drosophila genome throughout evolution. (2008)
  • Author(s): Siomi, H., et al.
  • Journal Title: Curr Opin Genet Dev 18 Pages: 181-187
  • Peer Reviewed
• [Journal Article] How selfish retrotransposons are silenced in Drosophila germline andsomatic cells. (2008)
  • Author(s): Siomi, MC., et al.
  • Journal Title: FEBS Lett. 582 Pages: 2473-2478
  • Peer Reviewed
• [Journal Article] How to define targets for small guide RNAs in RNA silencing : a biochemical approach. (2008)
  • Author(s): Siomi, MC., et al.
  • Journal Title: Methods in Enzymology 449 Pages: 345-355
  • Peer Reviewed
• [Journal Article] Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila.
  • Author(s): Nishida, KM., Miyoshi, K., Ogino, A., Miyoshi, T., Siomi, H. and Siomi, MC.
  • Journal Title: Mol Cell Volume: 49 Pages: 6-6
  • Peer Reviewed
• [Presentation] Small RNA-mediated transposon silencing (2013)
  • Author(s): H. Siomi
  • Organizer: Radiation Effects research Foundation, International Workshop: RERF Radiation Research in the Post-genome Era
  • Place of Presentation: Hiroshima, Japan
  • Invited
• [Presentation] Transposon Silencing mediated by small RNAs in the Drosophila overy (2012)
  • Author(s): H.Siomi
  • Organizer: The 5^<th> International Symposium on Nanomedicine
  • Place of Presentation: 名古屋大学ESホール(招待講演)
  • Year and Date: 2012-03-15
• [Presentation] 転移因子の抑制機構とRNAサイレンジング (2012)
  • Author(s): 塩見春彦
  • Organizer: 第8回宮崎サイエンスキャンプ
  • Place of Presentation: シェラトン・リゾート・フェニックス・シーガイヤ宮崎、宮崎(招待講演)
  • Year and Date: 2012-02-18
• [Presentation] piRNA biogenesis in the Drosophila ovary. (2012)
  • Author(s): H. Siomi
  • Organizer: Cell Symposia: Functional RNAs
  • Place of Presentation: Sitges, Spain
• [Presentation] piRNA biogenesis in the Drosophila ovary (2012)
  • Author(s): H. Siomi
  • Organizer: 63^<rd> Fujihara Seminar, "A new horizon of retroposon research"
  • Place of Presentation: Kyoto
• [Presentation] Somatic piRNA biogenesis. (2012)
  • Author(s): H. Siomi
  • Organizer: The 3^<rd>Shanghai International Conference of Epigenetics in Development and Diseases/The 7^<th> Annual Conference of Asian Epigenome Alliance/Genome Medicine Workshop on Epigenetic (-moic)s in Diseases
  • Place of Presentation: Shanghai, China
• [Presentation] Biogenesis of PIWI-interaction RNAs nd transposon silencing in Drosophila (2012)
  • Author(s): H. Siomi
  • Organizer: The 3rd Shanghai International Conference of Epigenetics in Development and Diseases/The 7th Annual Conference of Asian Epigenome Alliance/Genome Medicine Workshop on Epigenetic (-moic)s in Diseases
  • Place of Presentation: Shanghai, China
  • Invited
• [Presentation] Small RNA-mediated silencing of transposable elements in the Drosophila ovary (2012)
  • Author(s): H. Siomi
  • Organizer: Joint meeting of the 45th annual meeting of the Japanese Society of Developmental Biologists & the 64th annual meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe, Japan
  • Invited
• [Presentation] Maelstrom functions in both transposon silencing and microtubule organization in the Drosophila ovary (2012)
  • Author(s): H. Siomi
  • Organizer: The 22nd CDB meeting, RNA sciences in cell and developmental biology II
  • Place of Presentation: Kobe, Japan
  • Invited
• [Presentation] piRNA-mediated silencing of transposable elements in the Drosophila ovary (2012)
  • Author(s): H. Siomi
  • Organizer: The 63rd Fujihara Seminar, “A new horizon of retroposon research”
  • Place of Presentation: Kyoto, Japan
  • Invited
• [Presentation] Transposon silencing mediated by germline-specific small RNAs (piRNAs) in Drosophila. (2012)
  • Author(s): H. Siomi
  • Organizer: The 84th annual Meeting of the Genetics Society of Japan, Symposium: “Epigenomic regulation of cell fate determination and homeostasis”
  • Place of Presentation: Fukuoka, Japan
  • Invited
• [Presentation] microRNA、最近の話題 (2011)
  • Author(s): 塩見春彦
  • Organizer: Advans研究会2011
  • Place of Presentation: ウェスティン名古屋キャッスルホテル、名古屋(招待講演)
  • Year and Date: 2011-12-17
• [Presentation] RNAによるRNAの制御 (2011)
  • Author(s): 塩見春彦
  • Organizer: 第12回KMU研究推進セミナー
  • Place of Presentation: 金沢医科大学(招待講演)
  • Year and Date: 2011-11-28
• [Presentation] microRNA、最近の話題 (2011)
  • Author(s): 塩見春彦
  • Organizer: 第20回健康医科学産業推進協議会
  • Place of Presentation: 東海大学校友会館、東京(招待講演)
  • Year and Date: 2011-11-25
• [Presentation] 小分子RNAによるトランスポゾンの制御 (2011)
  • Author(s): 塩見春彦
  • Organizer: 第9回RCGMフロンティアシンポジウム「遺伝子発現制御とRNA-基礎と臨床をつなぐ分子」
  • Place of Presentation: 埼玉医科大学(招待講演)
  • Year and Date: 2011-11-03
• [Presentation] 核酸レベルの自己-非自己認識機構 (2011)
  • Author(s): 塩見春彦
  • Organizer: 藤田保健衛生大学医学セミナー
  • Place of Presentation: 藤田保健衛生大学、名古屋(招待講演)
  • Year and Date: 2011-10-14
• [Presentation] 機能のわからないタンパク質に機能を構造から予想する (2011)
  • Author(s): 塩見春彦
  • Organizer: 科学技術振興機構研究開発戦略センター俯瞰ワークショップ「構造生命科学」
  • Place of Presentation: 科学技術振興機構研究開発戦略センター、東京(招待講演)
  • Year and Date: 2011-09-10
• [Presentation] RNAとエピゲノム形成 (2011)
  • Author(s): 塩見春彦
  • Organizer: ヒューマンサイエンス振興財団
  • Place of Presentation: ヒューマンサイエンス振興財団、東京(招待講演)
  • Year and Date: 2011-07-15
• [Presentation] Cytoplasmic Yb bodies : sites for both piRNA biogenesis and piRNA-Piwi complex (piRISC) assembly in Drosophila ovarian somatic cells (2011)
  • Author(s): H.Siomi
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 北海道大学クラーク会館学術交流会館(招待講演)
  • Year and Date: 2011-06-29
• [Presentation] miRNA研究の現状:発現、機能、疾患 (2011)
  • Author(s): 塩見春彦
  • Organizer: 第35回皮膚科免疫セミナー
  • Place of Presentation: 品川
  • Year and Date: 2011-03-05
• [Presentation] 小分子RNAの一生 (2011)
  • Author(s): 塩見春彦
  • Organizer: 岐阜大学大学院連合創薬医療情報研究科
  • Place of Presentation: 岐阜
  • Year and Date: 2011-01-28
• [Presentation] a piRNA gene required for MTOC formation in the Drosophila oogenesis. CGC/CBG meeting Epigenetics and non-coding RNAs (2011)
  • Author(s): H. Siomi.
  • Organizer: Koninklijk Instituut voor de Tropen
  • Place of Presentation: Amsterdam
• [Presentation] Maelstrom, a component of the piRNA pathway, coordinates microubule organization during Drosophila oogenesis through interaction with components of the MTOC (2011)
  • Author(s): H.Siomi
  • Organizer: CGC/CBG meeting Epigenetics and non-coding RNAs
  • Place of Presentation: Koninklijk Instituut voor de Tropen, Mauritskade 63, Amsterdam Nederland(招待講演)
• [Presentation] RNAサイレンシング機構とトランスポゾンの抑制 (2010)
  • Author(s): 塩見春彦
  • Organizer: 北海道大学大学院医学研究科第8回分子生物学セミナー
  • Place of Presentation: 札幌
  • Year and Date: 2010-11-25
• [Presentation] piRNA biogenesis in the Drosophila ovarian somatic cells. (2010)
  • Author(s): H. Siomi
  • Organizer: Memorial Sloan-Kettering Cancer Institute
  • Place of Presentation: New York
  • Year and Date: 2010-11-04
• [Presentation] Yb body : a place where Piwi meets piRNAs. (2010)
  • Author(s): H.Siomi
  • Organizer: Memorial Sloan-Kettering Cancer Institute
  • Place of Presentation: New York
  • Year and Date: 2010-11-04
• [Presentation] piRNA biogenesis in the Drosophila ovary (2010)
  • Author(s): H. Siomi
  • Organizer: The New York Academy of Sciences, " Piwi-interacting RNAs (piRNAs), the guardians of the germ-line stem cell genome - Biogenesis and Function-
  • Place of Presentation: New York
  • Year and Date: 2010-11-03
• [Presentation] Piwi-interacting RNAs (piRNAs), the guardians of the germ-line stem cell genome ?Biogenesis and Function- (2010)
  • Organizer: The New York Academy of Sciences
  • Place of Presentation: New York
  • Year and Date: 2010-11-03
• [Presentation] Yb body : a place where Piwi meets piRNAs. (2010)
  • Author(s): H.Siomi
  • Organizer: The New York Academy of Sciences, Piwi-interacting RNAs (piRNAs) the guardians of the germ-line stem cell genome -Biogenesis and Function-
  • Place of Presentation: New York
  • Year and Date: 2010-11-03
• [Presentation] Primary piRNA biogenesis occurs through cytoplasmic Yb bodies in Drosophila ovarian somatic cells (2010)
  • Author(s): H.Siomi
  • Organizer: International centre for genetic engineering and biotechnology (ICGEB)-European Science Foundation (ESF) Workshop on RNA processing in biology and medicine
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-10-20
• [Presentation] But it can't all just be luck (2010)
  • Author(s): 塩見春彦
  • Organizer: RNAフロンティアミーティング2010
  • Place of Presentation: 裾野
  • Year and Date: 2010-09-27
• [Presentation] miRNA研究の現状 (2010)
  • Author(s): 塩見春彦
  • Organizer: 第51回日本組織細胞化学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-05
• [Presentation] RNA silencing : functions and dysfunctions (2010)
  • Author(s): 塩見春彦
  • Organizer: 第50回日本先天異常学会学術集会
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2010-07-08
• [Presentation] 核酸レベルの「自己・非自己」認識機構とRNAサイレンシング (2010)
  • Author(s): 塩見春彦
  • Organizer: JSTさきがけ講演会
  • Place of Presentation: 東京大学医科学研究所
  • Year and Date: 2010-07-07
• [Presentation] Endogenous siRNAs in Drosophila : biogenesis, function, and its implication in human disease. (2010)
  • Author(s): H.Siomi
  • Organizer: International Symposium on Control of Gene Expression and Cancer, dedicated to the 20^<th> anniversary of the Institute of Gene Biology
  • Place of Presentation: Russian Academy of Sciences, Moscow, Russia
  • Year and Date: 2010-06-22
• [Presentation] How are piRNAs produced in the Drosophila ovary? (2010)
  • Author(s): H. Siomi
  • Organizer: "Bingzhi Forum"speaker at Institute of Zoology, Chinese Academy of Sciences
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-06-15
• [Presentation] "Bingzhi Forum" speaker at Institute of Zoology (2010)
  • Organizer: Chinese Academy of Sciences
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-06-15
• [Presentation] Characterization of RNA silencing pathways in Drosophila gonad. (2010)
  • Author(s): H Siomi
  • Organizer: "Bingzhi Forum" speaker at Institute of Zoology
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-06-15
• [Presentation] RNAサイレンシングと転移因子の抑制 (2010)
  • Author(s): 塩見春彦
  • Organizer: 第17回自己抗体と自己免疫シンポジウム-microRNAと自己免疫-
  • Place of Presentation: 東京
  • Year and Date: 2010-02-26
• [Presentation] A link between RNA silencing and germ cell development in Drosophila (2010)
  • Author(s): 塩見春彦
  • Organizer: 第2回 NAGOYAグローバルリトリート
  • Place of Presentation: 愛知
  • Year and Date: 2010-02-26
• [Presentation] A link between RNA silencing and germ cell development in Drosophila. (2010)
  • Author(s): 塩見春彦
  • Organizer: The GCOE Keio Univ.& Karolinska Institute joint meeting
  • Place of Presentation: 東京
  • Year and Date: 2010-02-25
• [Presentation] Link between RNA silencing and transposon repression. (2010)
  • Author(s): 塩見春彦
  • Organizer: Plenary Lecture, RNA Symposium 2010(the 3^<rd> RNA Molecular Biology meeting of Taiwan)
  • Place of Presentation: Tainan, Taiwan
  • Year and Date: 2010-02-08
• [Presentation] Biochemical characterizations of piRNA genesis (2010)
  • Author(s): H. Siomi
  • Organizer: Genetics Seminar Program
  • Place of Presentation: Yale University School of Medicine, New Haven
  • Year and Date: 2010-01-12
• [Presentation] Genetics Seminar Program (2010)
  • Organizer: Yale University School of Medicine
  • Place of Presentation: New Haven
  • Year and Date: 2010-01-12
• [Presentation] Biochemical characterization of PIWI proteins in fly gonads. (2010)
  • Author(s): 塩見春彦
  • Organizer: Genetics Seminar Program, Yale University School of Medicine
  • Place of Presentation: New Haven, USA
  • Year and Date: 2010-01-12
• [Presentation] Endo-siRNA production and somatic silencing of transposons (2010)
  • Author(s): H. Siomi
  • Organizer: International symposium on Control of Gene Expression and Cancer, dedicated to the 20th anniversary of the Institute of Gene Biology, Russian Academy of Sciences
  • Place of Presentation: Moscow, Russia
• [Presentation] piRNA-mediated transposon silencing. Plenary Lecture (2010)
  • Author(s): H. Siomi
  • Organizer: RNA Symposium 2010 (the 3rd RNA molecular Biology meeting of Taiwan)
  • Place of Presentation: National Cheng Kung University, Tainan, Taiwan
• [Presentation] International Symposium on Control of Gene Expression and Cancer (2010)
  • Organizer: dedicated to the 20th anniversary of the Institute of Gene Biology, Russian Academy of Sciences
  • Place of Presentation: Moscow, Russia
• [Presentation] Plenary Lecture, RNA Symposium 2010 (2010)
  • Organizer: (the 3rd RNA molecular Biology meeting of Taiwan), National Cheng Kung University
  • Place of Presentation: Tainan, Taiwan
• [Presentation] A link between RNA silencing and Germline Development in Drosophila. (2009)
  • Author(s): 塩見春彦
  • Organizer: The 4^<th> International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: 沖縄
  • Year and Date: 2009-12-02
• [Presentation] 小分子RNA似よる遺伝子発現抑制:miRNAの機能と機能不全を中心に (2009)
  • Author(s): 塩見春彦
  • Organizer: 第16回関東臨床皮膚疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-24
• [Presentation] ショウジョウバエをモデル動物とした脆弱X症候群発症機構の研究 (2009)
  • Author(s): 塩見春彦
  • Organizer: シンポジウム 遺伝性精神遅滞「脆弱X症候群」の現状と進歩
  • Place of Presentation: 東京
  • Year and Date: 2009-11-14
• [Presentation] 小分子RNA似よる遺伝子発現抑制:疾患および幹細胞生物学への関与 (2009)
  • Author(s): 塩見春彦
  • Organizer: 第6回FAT-DMミーティング
  • Place of Presentation: 東京
  • Year and Date: 2009-10-28
• [Presentation] Biogenesis and Functions of piRNA in Germline Cells. (2009)
  • Author(s): 塩見春彦
  • Organizer: RNAi Asia 2009 & RNAi China 2009
  • Place of Presentation: Kunshan, China
  • Year and Date: 2009-10-16
• [Presentation] On the road to reading the RNA-interference code (2009)
  • Author(s): 塩見春彦
  • Organizer: 第294回つくば分子生命科学セミナー
  • Place of Presentation: 筑波
  • Year and Date: 2009-10-07
• [Presentation] 小分子RNAによる幹細胞制御 (2009)
  • Author(s): 塩見春彦
  • Organizer: 第1回日本RNAi研究会
  • Place of Presentation: 広島
  • Year and Date: 2009-08-28
• [Presentation] RNA silencing : functions and dysfunctions. (2009)
  • Author(s): 塩見春彦
  • Organizer: The 6^<th> Meeting of Bone Biology Forum.
  • Place of Presentation: 静岡
  • Year and Date: 2009-08-22
• [Presentation] RNA silencing : small RNAs as regulators of gene expression. (2009)
  • Author(s): 塩見春彦
  • Organizer: The Keio-Lund joint meeting 2009
  • Place of Presentation: 東京
  • Year and Date: 2009-08-04
• [Presentation] 生殖細胞形成とRNAサイレンシング (2009)
  • Author(s): 塩見春彦
  • Organizer: 第33回阿蘇シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2009-07-31
• [Presentation] 小分子RNAによる遺伝子発現制御機構 (2009)
  • Author(s): 塩見春彦
  • Organizer: 第27回日本骨代謝学会学術集会、Meet-the-Expert 4
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-25
• [Presentation] ショウジョウバエ生殖幹細胞形成とRNAサイレンシング (2009)
  • Author(s): 塩見春彦
  • Organizer: 東京工業大学大学院生命理工学研究科セミナー
  • Place of Presentation: 横浜
  • Year and Date: 2009-07-23
• [Presentation] 小分子RNAによる遺伝子発現制御とその機能異常による疾患 (2009)
  • Author(s): 塩見春彦
  • Organizer: 癌治療開発&先端医療開発を目指した最前線セミナー、基調講演
  • Place of Presentation: 東京
  • Year and Date: 2009-05-19
• [Presentation] ショウジョウバエ生殖幹細胞形成とRNAサイレンシング (2009)
  • Author(s): 塩見春彦
  • Organizer: 東京大学大学院工学研究科セミナー
  • Place of Presentation: 東京
  • Year and Date: 2009-05-19
• [Presentation] ショウジョウバエ生殖幹細胞形成とRNAサイレンシング (2009)
  • Author(s): 塩見春彦
  • Organizer: 日本分子生物学会 第9回春季シンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-05-12
• [Presentation] RNAサイレンシングと生殖幹細胞形成 (2009)
  • Author(s): 塩見春彦
  • Organizer: 第2回臓器間ネットワークと欠陥生物学から読み解くメタボリックシンドローム研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-09
• [Presentation] 小分子RNAによる遺伝子発現制御機構:その機能と異常 (2009)
  • Author(s): 塩見春彦
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬
  • Year and Date: 2009-04-24
• [Presentation] RNAサイレンシングと生殖幹細胞 (2009)
  • Author(s): 塩見春彦
  • Organizer: 第41回ケムバイオシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] 小分子RNAによるゲノムの品質管理機構 (2009)
  • Author(s): 塩見春彦
  • Organizer: 順天堂大学第25回細胞生物学セミナー
  • Place of Presentation: 東京
  • Year and Date: 2009-01-21
• [Presentation] Links between RNAi-related pathways and retrotransposon silencing in Drosophila. (2008)
  • Author(s): H. Siomi
  • Organizer: National Institute of Biological Sciences
  • Place of Presentation: Beijing, China
  • Year and Date: 2008-12-18
• [Presentation] Links between RNAi-related pathways and retrotransposon silencing. (2008)
  • Author(s): H. Siomi
  • Organizer: International symposium on the Expanding World of Post-transcriptional Regulation on Gene Expression at Tokyo Medical and Dental university
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-13
• [Presentation] RNA silencing : 核酸を標的認識分子として用いた免疫機構 (2008)
  • Author(s): 塩見春彦
  • Organizer: 第38回日本免疫学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] RNAサイレンシング : 小分子RNAが仲介する遺伝子発現制御機構 (2008)
  • Author(s): 塩見春彦
  • Organizer: 第18回アンチセンスシンポジウム
  • Place of Presentation: 岐阜
  • Year and Date: 2008-11-18
• [Presentation] 小分子non-coding RNAによる転移因子の抑制 (2008)
  • Author(s): 塩見春彦
  • Organizer: 千里ライフサイエンスセミナー
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-11
• [Presentation] RNAサイレンシング : 小分子RNAによる遺伝子発現制御機構 (2008)
  • Author(s): 塩見春彦
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-30
• [Presentation] 小分子RNAによる遺伝子発現制御機構 : その機能と異常 (2008)
  • Author(s): 塩見春彦
  • Organizer: 発生工学・疾患モデル研究会第70回定例会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-10
• [Presentation] RNAi分子経路とレトロトランスポゾン抑制経路との関連(*ワークショップオーガナイザー) (2008)
  • Author(s): 塩見春彦
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-04
• [Presentation] 内在性小分子RNAによる転移因子の抑制 (2008)
  • Author(s): 塩見春彦
  • Organizer: 第20回高遠シンポジウム
  • Place of Presentation: 高遠、長野
  • Year and Date: 2008-08-21
• [Presentation] RiboClub 2008 Opening Session Program ‘Tiny RNAs-big players in the RNA world', (2008)
  • Organizer: Sherbrooke, Quebec,
  • Place of Presentation: Canada
• [Presentation] Links between RNA silencing and transposon suppression. (2008)
  • Author(s): H. Siomi
  • Organizer: The 65^<th> KSBMB annual meeting
  • Place of Presentation: Seoul, Korea
• [Presentation] Different types of small RNAs and Argonautes are utilized to repress retrotransposons in germline and somatic cells. (2008)
  • Author(s): H. Siomi
  • Organizer: The 41^<st> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima, Japan
• [Presentation] RNAi-related pathways repress retrotransposons in germline and somatic cells in flies. (*Symposium Organizer) (2008)
  • Author(s): H. Siomi
  • Organizer: The 21^<st> Naito Conference, Nuclear Dynamics and RNA [I]
  • Place of Presentation: Yatsugatake, Japan
• [Presentation] RNAi machineries that repress retrotransposons in Drosophila. (2008)
  • Author(s): H. Siomi
  • Organizer: RiboClub 2008
  • Place of Presentation: Sherbrooke, Quebec, Canada
• [Presentation] Links between RNA silencing and retrotransposon suppression. (2008)
  • Author(s): H. Siomi
  • Organizer: 20^<th> FAOBMB Taipei Conference
  • Place of Presentation: Yang-Ming University, Taiwan
• [Presentation] ショウジョウバエAG02に結合する内在性小分子RNAの解析 (2008)
  • Author(s): 塩見春彦
  • Organizer: 第10回RNAミーティング
  • Place of Presentation: 札幌
• [Book] Trinucleotide Repeat Protocols (Methods in Molecular Biology) (2013)
  • Author(s): Miyoshi, K
  • Total Pages: 10
  • Publisher: Springer
• [Book] PIWI proteins and their Slicer activity in piRNA biogenesis and transposon silencing. The Enzymes Vol. 32: Part B: RNA endonucleases in RNAi and microRNA metabolism and their partners (2012)
  • Author(s): Sato, K., and Siomi, H(eds., F. Tamanoi, F. Guo, and G. Chanfreau)
  • Publisher: Elsevier
• [Book] PIWI proteins and their Slicer activity in piRNA biogenesis and transposon silencing. The Enzymes Vol. 32: Part B: RNA endonucleases in RNAi and microRNA metabolism and their partners (2012)
  • Author(s): Sato, K
  • Total Pages: 25
  • Publisher: Elsevier
• [Book] Biochemical analyses of endogenous Argonaute complexes immunopurified with anti-Argonaute monoclonal antibodies. Methods in Molecular Biology 725, Argonaute Proteins: Methods and Protocols (2011)
  • Author(s): Miyoshi, K., Okada, T., Siomi, H. and Siomi, MC.
• [Book] 21世紀の分子生物学 (2011)
  • Author(s): 日本分子生物学会
  • Publisher: 東京化学同人
• [Book] The key features of RNA silencing. pp1-28. In RNA Technologies and Their Applications (2010)
  • Author(s): Saito, K., Miyoshi, K., Siomi, MC., and Siomi, H(eds., V. Erdmann and J. Barciszewski)
  • Publisher: Springer-Verlag Berlin Heidelberg
• [Book] RNA Technologies and Their Applications(eds., V.Erdmann and J.Barciszewski)(The key features of RNA silencing.) (2010)
  • Author(s): Saito, K., Miyoshi, K., Siomi, MC., Siomi, H.
  • Publisher: Springer-Verlag Berlin Heidelberg
• [Book] The Key features of RNA silencing. In RNA Technologies and Their Applications (2010)
  • Author(s): Saito, K.
  • Publisher: Springer.(In press)
• [Book] Methods in Molecular Biology (2010)
  • Author(s): Miyoshi, K.
  • Publisher: HUMANA PRESS(In press)
• [Book] How to define targets for small guide RNAs in RNA silencing: a biochemical approach. Methods in Enzymology (2008)
  • Author(s): Siomi, MC., Nishida, KM., and Siomi, H.
• [Book] In vitroRNA cleavage assay for Argonaute-family proteins. Methods in Molecular Biology (2008)
  • Author(s): Miyoshi, K., Uejima, H., Nagami, T., Siomi, H., and Siomi, MC
• [Remarks]
  • URL: http://www.siomilab.med.keio.ac.jp/
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/dmb/sindex.html
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/dmb/sindex.html
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/dmb/sindex.html
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/dmb/sindex.html