Molecular mechanism and regulation of assembly and remodeling of proteins

• Project/Area Number: 20227004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: ARAKI Hiroyuki 国立遺伝学研究所, 細胞遺伝研究系, 教授 (20151160)
• Project Period (FY): 2008-05-12 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥199,810,000 (Direct Cost: ¥153,700,000、Indirect Cost: ¥46,110,000); Fiscal Year 2012: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000); Fiscal Year 2011: ¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000); Fiscal Year 2010: ¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000); Fiscal Year 2009: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000); Fiscal Year 2008: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
• Keywords: リモデリング / DNA複製 / 複製開始 / DNA合成 / 細胞周期 / 染色体DNA / DNAヘリカーゼ / 酵母 / 染色体

Research Abstract

Many proteins assemble and form complexes at DNA replication origins. The complexes are subsequently remodeled to initiate DNA replication. This study newly identified the pre-LC and the Sld3-Sld7 protein complexes of budding yeast. These complexes recruit two replication factors, Cdc45 and GINS to origins and remodel replicative helicase, Mcm2-7 to an active form, which unwinds double-stranded DNA to single-stranded DNA to perform DNA replication.

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Research Products

• [Journal Article] Concerted interaction between origin recognition complex (ORC), nucleosomes, and replication origin DNA ensures stable ORC-origin binding (2013)
  • Author(s): Hizume, K., Yagura, M. and Araki, H.
  • Journal Title: Genes Cells Volume: 18 Issue: 9 Pages: 764-779
  • DOI: 10.1111/gtc.12073
  • Peer Reviewed
• [Journal Article] Kinetochores coordinate pericentromeric cohesion and early DNA replication by Cdc7-Dbf4 kinase recruitment (2013)
  • Author(s): Natsume, T., Muller, C. A., Katou, Y., Retkute, R., Gierliński, M., Araki, H., Blow, J.J., Shirahige, K., Nieduszynski, C.A. and Tanaka, T.U.
  • Journal Title: Mol. Cell Volume: 50 Issue: 5 Pages: 661-674
  • DOI: 10.1016/j.molcel.2013.05.011
  • Peer Reviewed
• [Journal Article] Efficient initiation of DNA replication in eukaryotes requires Dpb11/TopBP1-GINS interaction (2013)
  • Author(s): Tanaka, S., Komeda, Y., Umemori, T., Kubota, Y., Takisawa, H. and Araki, H.
  • Journal Title: Mol. Cell. Biol Volume: 33 Issue: 13 Pages: 2614-2622
  • DOI: 10.1128/mcb.00431-13
  • Peer Reviewed
• [Journal Article] Loading and activation of DNA replicative helicases: the key step of initiation of DNA replication. (2013)
  • Author(s): Li, Y. and Araki, H.
  • Journal Title: Genes to Cells Volume: 18 Issue: 4 Pages: 266-277
  • DOI: 10.1111/gtc.12040
  • Peer Reviewed
• [Journal Article] Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing (2011)
  • Author(s): Tanaka, S., Nakano, R., Katou, Y., Shirahige, K. and Araki, H.
  • Journal Title: Curr. Biol Volume: 21 Issue: 24 Pages: 2055-2063
  • DOI: 10.1016/j.cub.2011.11.038
  • Peer Reviewed
• [Journal Article] Initiation of chromosomal DNA replication in eukaryotic cells; contribution of yeast genetics to the elucidation (2011)
  • Author(s): Araki, H
  • Journal Title: Genes & Genetic Systems Volume: 86 Issue: 3 Pages: 141-149
  • DOI: 10.1266/ggs.86.141
  • NAID: 10029678672
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Journal Article] Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance (2011)
  • Author(s): Tanaka, S. and Araki, H
  • Journal Title: PLoS Genet Volume: 7 Issue: 6 Pages: e1002136-e1002136
  • DOI: 10.1371/journal.pgen.1002136
  • Peer Reviewed
• [Journal Article] Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeas (2011)
  • Author(s): Tanaka T.、Umemori T.、Endo S.、Muramatsu S.、Kanemaki M.、Kamimura Y.、Obuse C.、Araki H.
  • Journal Title: The EMBO journal Volume: 30(10) Issue: 10 Pages: 2019-2030
  • DOI: 10.1038/emboj.2011.115
  • Peer Reviewed
• [Journal Article] Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance (2011)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: PLoS Genet. Volume: 7
  • Peer Reviewed
• [Journal Article] Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast. (2011)
  • Author(s): Tanaka, T., Umemori, T., Endo, S., Muramatsu, S., Kanemaki, M., Kamimura, Y., Obuse, C., Araki, H.
• [Journal Article] CDK-dependent complex formation between replication proteins, Dpb11, Sld2, Polε and GINS in budding yeast (2010)
  • Author(s): Muramatsu, S., Hirai, K., Tak, Y-S., Kamimura, Y. and Araki, H
  • Journal Title: Genes & Dev Volume: 24 Issue: 6 Pages: 602-612
  • DOI: 10.1101/gad.1883410
• [Journal Article] Cyclin-dependent kinase-dependent initiation of chromosomal DNA replication (2010)
  • Author(s): Araki, H
  • Journal Title: Curr. Opin. Cell Biol Volume: 22 Issue: 6 Pages: 766-771
  • DOI: 10.1016/j.ceb.2010.07.015
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases (2010)
  • Author(s): Tanaka, S. and Araki, H
  • Journal Title: Chromosoma Volume: 119 Issue: 6 Pages: 565-574
  • DOI: 10.1007/s00412-010-0291-8
  • Peer Reviewed
• [Journal Article] CDK-dependent complex formation between replication proteins (2010)
  • Author(s): Muramatsu, S., Hirai, K., Tak, Y-S., Kamimura, Y., Araki, H.
  • Journal Title: Dpbll, Sld2, Polε and GINS in budding yeast.Genes & Dev. 24 Pages: 602-612
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinase-dependent initiation of chromosomal DNA replication. (2010)
  • Author(s): Araki H.
  • Journal Title: Curt.Opin.Cell Biol. 22 Pages: 766-771
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases. (2010)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: Chromosoma 119 Pages: 565-574
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinase-dependent initiation of chromosomal DNA replication (2010)
  • Author(s): Hiroyuki Araki
  • Journal Title: Curr.Opin.Cell Biol. Volume: 22 Pages: 766-771
  • Peer Reviewed
• [Journal Article] CDK-dependent complex formation between replication proteins, Dpb11, Sld2, Polε and GINS in budding yeast (2010)
  • Author(s): Muramatsu, S., Hirai, K., Tak, Y-S., Kamimura, Y., Araki, H
  • Journal Title: Genes & Dev. Volume: 24 Pages: 602-612
  • Peer Reviewed
• [Journal Article] Regulatory mechanism of the initiation step of DNA replication by CDK in budding yeast (2009)
  • Author(s): Araki, H
  • Journal Title: Biochimica et Biophysica Acta Volume: 1804 Issue: 3 Pages: 520-523
  • DOI: 10.1016/j.bbapap.2009.10.020
• [Journal Article] Regulatory mechanism of the initiation step of DNA replication by CDK in budding yeast. (2009)
  • Author(s): Araki H.
  • Journal Title: Biochimica et Biophysica Acta 1804 Pages: 520-523
  • Peer Reviewed
• [Journal Article] 真核生物染色体DNAの複製開始を担う複製蛋白質複合体の形成 (2009)
  • Author(s): 荒木弘之
  • Journal Title: 蛋白質核酸酵素 54 Pages: 350-355
• [Journal Article] 真核細胞染色体DNA複製開始反応とCDKによる制御 (2008)
  • Author(s): 荒木弘之
  • Journal Title: 細胞工学 27 Pages: 985-991
• [Presentation] Formation of active replicative helicase in budding yeast (2013)
  • Author(s): Araki, H., Yagura, M., Makino, N., Tanaka, Y., Itou, H., Hizume, K. and Tanaka S
  • Organizer: 2013 Cold Spring Harbor Laboratory Meeting on Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2013-09-11
• [Presentation] Establishment of replication forks at origins, Genome Instability (2013)
  • Author(s): Araki, H.
  • Organizer: Evolution and Human Diseases
  • Place of Presentation: St. Petersburg, Russia
  • Year and Date: 2013-06-15
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication (2013)
  • Author(s): Araki, H., Makino, N., Yagura, M., Tanaka, Y., Hizume, K. and Tanaka, S.
  • Organizer: Joint Conference of HGM 2013 and 21^<st> International Congress of Genetics
  • Place of Presentation: Singapore
  • Year and Date: 2013-04-13
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in eukaryotic cells (2013)
  • Author(s): Araki H., Makino N., Yagura M., Tanaka Y., Hizume K., and Tanaka S.
  • Organizer: 2013ヒトゲノム会議（HGM）と第21回国際遺伝学会議（ICG)の合同会議
  • Place of Presentation: シンガポール
  • Invited
• [Presentation] Establishment of replication forks at origins (2013)
  • Author(s): Araki, H.
  • Organizer: ゲノム不安定性・進化・ヒトの疾病会議
  • Place of Presentation: サンクトペテルブルグ (ロシア)
  • Invited
• [Presentation] Formation of active replicative helicase in budding yeast (2013)
  • Author(s): Araki H., Yagura M., Makino N., Tanaka Y., Itou H., Hizume K., and Tanaka S.
  • Organizer: 2013 Cold Spring Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY（USA）
  • Invited
• [Presentation] Dpb11/TopBP1-GINS interaction is required for efficient initiation of DNA replication (2013)
  • Author(s): Tanaka, S., Komeda,Y., Umemori,T., Kubota,Y., Takisawa,H., and Araki,H
  • Organizer: 2013 Cold Spring Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY（USA）
• [Presentation] DNA replication licensing on the chromatin fiber―Visualization by atomic force microscopy (2013)
  • Author(s): Hizume,K., Yagura,M.,and Araki,H.
  • Organizer: 2013 Cold Spring Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY（USA）
• [Presentation] In vitro study of the initiation of chromosomal DNA replication in budding yeast (2013)
  • Author(s): Yagura, M. Araki H.
  • Organizer: 2013 Cold Spring Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY（USA）
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in eukaryotic cells (2013)
  • Author(s): Araki H., Makino N., Yagura M., Tanaka Y., Hizume K., and Tanaka S.
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 出芽酵母染色体DNA の複製開始機構 (2012)
  • Author(s): 荒木弘之、牧野仁志穂、矢倉勝、田中尚美、遠藤静子、村松佐知子、日詰光治、田中誠司
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-13
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in budding yeast (2012)
  • Author(s): Araki, H.
  • Organizer: The 8^<th> 3R Symposium
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2012-11-27
• [Presentation] Initiation mechanism of chromosomal DNA replication (2012)
  • Author(s): Araki, H.
  • Organizer: BSCB/BSDB/JSDB-Joint Spring Meeting
  • Place of Presentation: Warwick, UK
  • Year and Date: 2012-04-16
• [Presentation] Initiation mechanism of chromosome replication (2012)
  • Author(s): Araki H.
  • Organizer: BSCB/BSDB/JSDB-Joint Spring Meeting
  • Place of Presentation: Warwick (UK)
  • Invited
• [Presentation] Inter-BRCT region of DPB11 is a conserved GINS interaction domain,which is important for the initiation of DNA replication. (2012)
  • Author(s): Tanaka,S.,Komeda,Y.,Umemori,T.,Kubota Y.,Takisawa, H.,Araki,H
  • Organizer: 2012 FASEB Summer Research Conferences
  • Place of Presentation: Steamboat Springs (USA)
• [Presentation] 出芽酵母Sld3-Sld7複合体によるDNA複製開始機構の解明 (2012)
  • Author(s): 牧野仁志穂、荒木弘之
  • Organizer: 酵母遺伝学フォーラム第45回研究報告会
  • Place of Presentation: 京都府宇治市
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in budding yeast (2012)
  • Author(s): Araki H.
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 兵庫県淡路市
  • Invited
• [Presentation] Efficient initiation of DNA replication requires evolutionarily conserved Dpb11-GINS interaction (2012)
  • Author(s): Tanaka S., Komeda Y., Umemori T., Kubota Y., Takisawa H. , Araki H.
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 兵庫県淡路市
• [Presentation] Concerted interaction between ORC, nucleosomes, and origin DNA ensures origin-specific ORC binding (2012)
  • Author(s): Hizume K., Yagura M., Araki H.
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 兵庫県淡路市
• [Presentation] In vitro study of the initiation of DNA replication in budding yeast (2012)
  • Author(s): Yagura M., Araki H.
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 兵庫県淡路市
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in budding yeast (2012)
  • Author(s): Araki H.
  • Organizer: 第1回 都医学研 国際シンポジウム「染色体サイクルの制御」
  • Place of Presentation: 東京都
  • Invited
• [Presentation] 試験管内反応系を用いた出芽酵母複製開始機構の解析 (2012)
  • Author(s): 矢倉勝, 荒木弘之
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] 出芽酵母Sld3-Sld7複合体によるDNA複製促進の分子機構 (2012)
  • Author(s): 牧野仁志穂, 荒木弘之
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] ORCの複製開始点特異的な結合に寄与する、ORC/nucleosome/複製開始点DNA間の協調的相互作用 (2012)
  • Author(s): 日詰光治, 矢倉勝, 荒木弘之
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] Inter-BRCT region of Dpb11 is a conserved GINS-interaction domain and is important for the initiation of DNA replication. (2012)
  • Author(s): 田中誠司, 米田弥生, 梅森稔子,久保田弓子, 滝澤温彦,荒木弘之
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Invited
• [Presentation] 出芽酵母染色体DNAの複製開始機構 (2012)
  • Author(s): 荒木弘之, 牧野仁志穂, 矢倉勝, 田中尚美, 遠藤静子, 村松 佐知子, 日詰光治, 田中誠司
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Invited
• [Presentation] Reconstitution of DNA replication licensing on the chromatin fiber. (2012)
  • Author(s): Hizume K., Yagura M., Araki H.
  • Organizer: アメリカ細胞生物学会（ASCB)
  • Place of Presentation: San Francisco (USA)
• [Presentation] Origin Association of Sld3, Sld7, and Cdc45 Proteins is a Key Step for Determination of Origin-Firing. Timing (2011)
  • Author(s): Seiji Tanaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-12-13
• [Presentation] Analysis of the interaction between chromatin and the origin recognition complex using atomic force microscopy (2011)
  • Author(s): Kohji Hizume
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] IN VITRO STUDY OF THE INITIATION OF DNA REPLICATION IN BUDDING YEAST (2011)
  • Author(s): Masaru Yagura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Characterization of the Sld3-Sld7 complex, an essential initiation factor for chromosome DNA replication in budding yeast (2011)
  • Author(s): Nishiho Makino
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in the budding yeast Saccharomyces cerevisiae (2011)
  • Author(s): 田中誠司
  • Organizer: 第21回DNA複製・組換え・ゲノム安定性制御ワークショップ
  • Place of Presentation: 福岡県福津
  • Year and Date: 2011-10-25
• [Presentation] 原子間力顕微鏡を用いたOrc/クロマチン相互作用の解析 (2011)
  • Author(s): 日詰光治
  • Organizer: 第21回DNA複製・組換え・ゲノム安定性制御ワークショップ
  • Place of Presentation: 福岡県福津
  • Year and Date: 2011-10-25
• [Presentation] 酵母染色体DNA複製開始におけるpre-LC複合体の役割 (2011)
  • Author(s): 荒木弘之
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-20
• [Presentation] Essential role of DNA polymerase epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Araki, H., Tanaka, Y., Yanagisawa, Y. and Endo, S.
  • Organizer: 2011 Cold Spring Harbor Laboratory Meeting on Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2011-09-08
• [Presentation] Essential role of DNA polymerase epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Hiroyuki Araki
  • Organizer: 2011 Cold Sprong Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY (USA)
  • Year and Date: 2011-09-06
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in budding yeast (2011)
  • Author(s): Seiji Tanaka
  • Organizer: 2011 Cold Sprong Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY (USA)
  • Year and Date: 2011-09-06
• [Presentation] IN VITRO STUDY OF THE INITIATION OF DNA REPLICATION IN BUDDING YEAST (2011)
  • Author(s): Masaru Yagura
  • Organizer: 2011 Cold Sprong Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY (USA)
  • Year and Date: 2011-09-06
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Hiroyuki Araki
  • Organizer: 25th International Conferenc on "Yeast Genetics and Molecular Biology"
  • Place of Presentation: Kortowo-Olsztyn (Poland)(招待講演)
  • Year and Date: 2011-07-07
• [Presentation] How replication proteins associate with origins to initiate chromosomal DNA replication (2011)
  • Author(s): Hiroyuki Araki
  • Organizer: 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: Windermere (UK)
  • Year and Date: 2011-04-10
• [Presentation] Yeast initiation proteins, Sld3, Sld7 and Cdc45, specifies the timing of origin-firing (2011)
  • Author(s): Seiji Tanaka
  • Organizer: 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: Windermere (UK)
  • Year and Date: 2011-04-10
• [Presentation] 真核生物の染色体DNA複製開始におけるSld3-Sld7複合体の役割 (2011)
  • Author(s): 牧野仁志穂
  • Organizer: 第12回静岡ライフサイエンスシンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-04
• [Presentation] Essential function of Pol epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Araki, H., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K. and Tanaka, S.
  • Organizer: Keystone Symposia "DNA Replication and Recombination"
  • Place of Presentation: Keystone, CO, USA
  • Year and Date: 2011-03-03
• [Presentation] Essential Function of Pol Epsilon at the Initiation Step of Chromosomal DNA Repication in Budding Yeast (2011)
  • Author(s): Hiroyuki Araki
  • Organizer: Keystone Symposia "DNA Replication and Recombination"
  • Place of Presentation: Colorado, USA
  • Year and Date: 2011-02-26
• [Presentation] Essential function of Pol epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Araki, H., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K., Tanaka, S.
  • Organizer: Keystone Symposia "DNA Replication and Recombination"
  • Place of Presentation: Keystone, CO, USA.
• [Presentation] CDKに依存した染色体DNA複製開始の分子機構 (2010)
  • Author(s): 荒木弘之
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Temporal regulation of activation of replication origins in the budding yeast Saccharomyces cerevisiae (2010)
  • Author(s): Seiji Tanaka
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] How replication proteins associate with origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki, H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K. and Tanaka, S
  • Organizer: The 7th 3R Symposium
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2010-10-30
• [Presentation] How replication proteins associate with origins to initiate chromosomal DNA replication (2010)
  • Author(s): Hiroyuki Araki
  • Organizer: 2010 3R(Replication, Recombination&Repair) Symposium
  • Place of Presentation: 富山
  • Year and Date: 2010-10-26
• [Presentation] Cdc7-Dbf4 kinase specifies early replication origins by regulating the association of low-abundant replication initiation factors in budding yeast (2010)
  • Author(s): Seiji Tanaka
  • Organizer: 2010 3R(Replication, Recombination&Repair) Symposium
  • Place of Presentation: 富山
  • Year and Date: 2010-10-26
• [Presentation] Fanctions of MCM10 in chromosomal DNA replication in budding yeast (2010)
  • Author(s): Masaru Yagura
  • Organizer: 2010 3R(Replication, Recombination&Repair) Symposium
  • Place of Presentation: 富山
  • Year and Date: 2010-10-26
• [Presentation] Protein assembly at the initiation stage of chromosomal DNA replication in budding yeast (2010)
  • Author(s): Kazuyuki Hirai
  • Organizer: 2010 3R(Replication, Recombination&Repair) Symposium
  • Place of Presentation: 富山
  • Year and Date: 2010-10-26
• [Presentation] 出芽酵母における複製開始メカニズム (2010)
  • Author(s): 荒木弘之
  • Organizer: 第82回日本遺伝学会木原賞受賞講演
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-19
• [Presentation] 真核生物におけるDNA複製制御機構とゲノム安定維持・高次複製制御機構 (2010)
  • Author(s): 田中誠司
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-09
• [Presentation] How replication proteins associate with and dissociate from origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki, H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., and Tanaka, S
  • Organizer: FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: Carefree, AZ, USA
  • Year and Date: 2010-08-12
• [Presentation] How replication proteins associate with and dissociate from origins to initiate chromosomal DNA replication (2010)
  • Author(s): Hiroyuki Araki
  • Organizer: 2010 FASEB Summer Research Conferences
  • Place of Presentation: Carefree, Arizona, USA
  • Year and Date: 2010-08-08
• [Presentation] How replication proteins associate with origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K., Tanaka, S.
  • Organizer: The 7th 3R Symposium
  • Place of Presentation: Toyama, Japan.
• [Presentation] How replication proteins associate with and dissociate from origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., Tanaka, S.
  • Organizer: FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: Carefree, AZ, USA.
• [Presentation] CDK-dependent assembly of replication proteins at the initiation step of chromosomal DNA replication (2009)
  • Author(s): Araki, H., Hirai, K., Li, Y., Tanaka, T., Muramatsu, S., Tanaka, S
  • Organizer: 2009 Cold Spring Harbor Laboratory Meeting on Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2009-09-01
• [Presentation] CDK-dependent assembly of replication proteins at the initiation step of chromosomal DNA replication (2009)
  • Author(s): Araki H., Hirai, K., Li, Y., Tanaka, T., Muramatsu, S., Tanaka, S.
  • Organizer: Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA.
• [Presentation] CDK-dependent assembly of replication protein to initiate chromosomal DNA replication (2008)
  • Author(s): Hiroyuki Araki
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Regulation of the replication protein Sld2 and its role in stable genome maintenance. (2008)
  • Author(s): Seiji Tanaka
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] In vitro reconstitution of pre-CMG complex in budding yeast (2008)
  • Author(s): Yan Li
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 染色体DNAの複製開始期に形成されるタンパク質複合体の生化学的解析 (2008)
  • Author(s): 平井和之
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] CDK-dependent ASSEMBLY of replication proteins to initiate chromosomal DNA replication (2008)
  • Author(s): Hiroyuki Araki
  • Organizer: 第一回GCOE国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-10-25
• [Presentation] In vitro reconstitution of pre-CMG complex in budding yeast (2008)
  • Author(s): Yan Li
  • Organizer: 酵母遺伝学フォーラム第41回研究報告会
  • Place of Presentation: 札幌
  • Year and Date: 2008-09-10
• [Presentation] Regulation of the replication protein Sld2 and its role in stable genome maintenance (2008)
  • Author(s): Seiji Tanaka
  • Organizer: 酵母遺伝学フォーラム第41回研究報告会
  • Place of Presentation: 札幌
  • Year and Date: 2008-09-10
• [Presentation] 染色体DNAの複製開始期に形成されるタンパク質複合体の解析 (2008)
  • Author(s): 平井和之
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-04
• [Presentation] CDK-dependent Assembly of Replication Proteins to Initiate Chromosomal DNA Replication (2008)
  • Author(s): Hiroyuki Araki
  • Organizer: DNA REPLICATION and GENOME INTEGRITY 2008
  • Place of Presentation: CA, USA
  • Year and Date: 2008-07-19
• [Presentation] Molecular mechanism of the initiation step of chromosomal DNA replication in budding yeast (2008)
  • Author(s): Araki, H., Tanaka, T, Hirai, K., Tanaka, Y., Umemori, T., Yanagisawa, Y., Muramatsu, S. and Tanaka, S
  • Organizer: XX International Congress of Genetics
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2008-07-13
• [Presentation] Molecular Mechanism of the Initiation Step of Chromosomal DNA Replication in Budding Yeast (2008)
  • Author(s): Araki H.
  • Organizer: Molecular Mechanism of the Initiation Step of Chromosomal DNA Replication in Budding Yeast
  • Place of Presentation: Belrin, Germany
  • Year and Date: 2008-07-13
• [Presentation] 染色体DNAの複製機構を探る (2008)
  • Author(s): 荒木弘之
  • Organizer: 第11回真核微生物交流会
  • Place of Presentation: 東広島市
  • Year and Date: 2008-07-04
• [Presentation] CDK-dependent regulation of chromosomal DNA replication (2008)
  • Author(s): Hiroyuki Araki
  • Organizer: FASEB 「Yeast Chromosome Structure, Replication & Segregation」
  • Place of Presentation: AZ, USA
  • Year and Date: 2008-06-25
• [Presentation] Periodic expression of Sld2 is important for DNA replication and genome integrity (2008)
  • Author(s): Seiji Tanaka
  • Organizer: Periodic expression of Sld2 is important for DNA replication and genome integrity
  • Place of Presentation: AZ, USA
  • Year and Date: 2008-06-25
• [Presentation] How CDKs trigger initiation of chromosomal DNA replication in budding yeast. (2008)
  • Author(s): Hiroyuki Araki
  • Organizer: 染色体サイクル「International Symposium on Chromosome Dynamics in Ise, 2008」
  • Place of Presentation: 志摩
  • Year and Date: 2008-05-28
• [Presentation] 真核生物染色体DNAの複製開始機構 (2008)
  • Author(s): 荒木弘之
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-27
• [Presentation] CDK-dependent initiation of chromosomal DNA replication in buddine yeast. (2008)
  • Author(s): Araki, H.
  • Organizer: Cold Spring Harbor Laboratory 「The Cell Cycle」
  • Place of Presentation: NY, USA
  • Year and Date: 2008-05-15
• [Presentation] Molecular mechanism of the initiation step of chromosomal DNA replication in budding yeast (2008)
  • Author(s): Araki H., Tanaka, T, Hirai, K., Tanaka, Y., Ememori, T., Yanagisawa, Y., Muramatsu, S., Tanaka, S.
  • Organizer: XX International Congress of Genetics
  • Place of Presentation: Berlin, Germany.
• [Book] Helicase activation and establishment of replication forks at chromosomal origins of replication. In DNA replication (eds. Bell, S. D., Mechali, M., and DePamphilis M. L.) (2013)
  • Author(s): Tanaka, S. and Araki, H
  • Publisher: Cold Spring Harbor Press, NY
• [Book] DNA replication (2013)
  • Author(s): Tanaka, S. and Araki, H.
  • Total Pages: 561
  • Publisher: Cold Spring Harbor Press
• [Remarks]
  • URL: http://www.nig.ac.jp/section/araki/araki-j.html
• [Remarks] Araki Lab.
  • URL: http://www.nig.ac.jp/labs/MicGen/index.html
• [Remarks] DNA複製：真核生物間で保存された、効率よい複製開始のためのタンパク間相互作用
  • URL: http://www.nig.ac.jp/Research-Highlights/1170/1233.html
• [Remarks] DNA複製のスタート地点の決定における、クロマチンの役割
  • URL: http://www.nig.ac.jp/Research-Highlights/1170/1270.html
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/MicGen/index.html
• [Remarks] ホームページ
  • URL: http://www.nig.ac.jp/section/araki/araki-j.html
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/MicGen/index.html