|Budget Amount *help
¥199,810,000 (Direct Cost: ¥153,700,000、Indirect Cost: ¥46,110,000)
Fiscal Year 2012: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000)
Fiscal Year 2011: ¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2010: ¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2009: ¥38,870,000 (Direct Cost: ¥29,900,000、Indirect Cost: ¥8,970,000)
Fiscal Year 2008: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Many proteins assemble and form complexes at DNA replication origins. The complexes are subsequently remodeled to initiate DNA replication. This study newly identified the pre-LC and the Sld3-Sld7 protein complexes of budding yeast. These complexes recruit two replication factors, Cdc45 and GINS to origins and remodel replicative helicase, Mcm2-7 to an active form, which unwinds double-stranded DNA to single-stranded DNA to perform DNA replication.