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Cell Turgor Measurement - Probe Electrospray Ionization (PESI) Mass Spectrometry for Cell Molecular Profiling Techniques

Research Project

Project/Area Number 20228004
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Agricultural environmental engineering
Research InstitutionEhime University

Principal Investigator

NONAMI Hiroshi  愛媛大学, 農学部, 教授 (00211467)

Co-Investigator(Kenkyū-buntansha) HIRAOKA Kenzo  山梨大学, クリーンエネルギー研究センター, 特命教授 (80107218)
Research Collaborator ERRA-BALSELLS Rosa  ブエノスアイレス大学, 基礎自然学部(大学間学術交流協定・学部間学術交流協定締結校), 教授
Project Period (FY) 2008-05-12 – 2013-03-31
Project Status Declined (Fiscal Year 2012)
Budget Amount *help
¥161,590,000 (Direct Cost: ¥124,300,000、Indirect Cost: ¥37,290,000)
Fiscal Year 2012: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2011: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2010: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2009: ¥73,580,000 (Direct Cost: ¥56,600,000、Indirect Cost: ¥16,980,000)
Fiscal Year 2008: ¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
Keywords生物環境調節 / 植物 / 細胞・組織 / 探針エレクトロスプレー / 質量分析 / スピーキング・セル・アブローヂ / 膨圧計測 / ブレヮシャーブローブ / 農業工学 / スピーキング・セル・アプローチ / プレッシャープローブ / プレッシャープロープ
Research Abstract

A cell pressure probe measures cell turgor of plants, and can extract cell solution directly from actively growing plants. The pressure probe technique and the probe electrospray ionization (PESI) mass spectrometry (MS) can be combined together in order to analyze cell molecular components in intact growing crops. When a small amount of engine oil additives was mixed with silicon oil in the pressure probe, ionization of cell metabolites was successfully conducted for MS analyses. By using physiological molecular information, environmental conditionscan be adjusted optimally to grow crops in plant growth factories.

Assessment Rating
Verification Result (Rating)

A

Report

(7 results)
  • 2013 Research Progress Assessment (Verification Result) ( PDF )
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (48 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (37 results) (of which Peer Reviewed: 37 results) Presentation (7 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Rosa Erra-Balsells (2012) Mass spectrometry of rhenium complexes: a comparative study by using LDI-MS, MALDI-MS, PESI-MS and ESI-MS2012

    • Author(s)
      GabrielaPetroselli,MridulKantiMandal,LeeChuinChen,GustavoTRuiz,EzequielWolcan,KenzoHiraoka,HiroshiNonami
    • Journal Title

      Journal of Mass Spectrometry

      Volume: Volume 47, Issue 3 Issue: 3 Pages: 313-321

    • DOI

      10.1002/jms.2965

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Online Electrospray Ionization Mass Spectrometric Monitoring of Protease-Catalyzed Reactions in Real Time2012

    • Author(s)
      Zhan Yu, Lee Chuin Chen, Mridul Kanti Mandal, Hiroshi Nonami, Rosa Erra-Balsells, Kenzo Hiraoka
    • Journal Title

      J.Am.Soc.Mass Spectrom.

      Volume: 23 Issue: 4 Pages: 728-735

    • DOI

      10.1007/s13361-011-0323-5

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of salt stress on physiological response of tomato fruit grown in hydroponic culture system2012

    • Author(s)
      Md.Mokter Hossain, Hiroshi Nonami
    • Journal Title

      Hort.Sci.(Prague)

      Volume: 39 Pages: 26-32

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Protein from Detergent Solutions by Probe Electrospray Ionization Mass Spectrometry (PESI-MS).2011

    • Author(s)
      M. K. Mandal, L. C. Chen, Z. Yu, H. Nonami, R. Erra-Balsells and K. Hiraoka
    • Journal Title

      Journal of Mass Spectrometry

      Volume: 46 Issue: 10 Pages: 967-975

    • DOI

      10.1002/jms.1977

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plant cell measurements with mass spectrometry for speaking cell approach in plant growth factories.2011

    • Author(s)
      Nonami, H., Gholipour, Y., Erra-Balsells, R
    • Journal Title

      IFAC Proceedings

      Volume: Volumes (IFAC-Papers Online) 18 (PART 1) Issue: 1 Pages: 1791-1795

    • DOI

      10.3182/20110828-6-it-1002.00450

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct Tissue and Cell Analysis of Tulip Plants by Combining Pressure Probe and UV-MALDI-MS2011

    • Author(s)
      Nonami, H., Gholipour, Y., Erra-Balsells, R.
    • Journal Title

      Acta Horticulturae

      Volume: 923 Pages: 53-57

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential and Exhaustive Ionization of Analytes with Different Surface Activity by Probe Electrospray Ionization2011

    • Author(s)
      Mridul Kanti Mandal, Lee Chuin Chen, Kenzo Hiraoka
    • Journal Title

      J.Am.Soc.Mass Spectrom.

      Volume: 22 Issue: 9 Pages: 1493-1500

    • DOI

      10.1007/s13361-011-0162-4

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased Ring-Shaped Chalkiness and Osmotic Adjustment when Growing Rice Grains under Foehn-Induced Dry Wind Condition2011

    • Author(s)
      Wada H., Nonami H., Yabuoshi Y., Maruyama A., Tanaka A., Wakamatsu K., Sumi T., Wakiyama Y., Ohuchida M., Morita S.
    • Journal Title

      Crop Sci.

      Volume: 51 Issue: 4 Pages: 1703-1715

    • DOI

      10.2135/cropsci2010.08.0503

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fruit growth of tomato associated with water uptake and cell expansion2011

    • Author(s)
      Md.Mokter Hossain, Hiroshi Nonami
    • Journal Title

      Journal of Agricultural Technology

      Volume: 7 Pages: 1049-1062

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diamond, Titanium Dioxide, Titanium Silicon Oxide, and Barium Strontium Titanium Oxide Nanoparticles as Matrixes for Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis of Carbohydrates in Plant Tissues2010

    • Author(s)
      Yousef Gholipour, Silvana L. Giudicessi, Hiroshi Nonami, and Rosa Erra-Balsells
    • Journal Title

      Analytical Chemistry

      Volume: 82 Issue: 13 Pages: 5518-5526

    • DOI

      10.1021/ac1003129

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time reaction monitoring by probe electrospray ionization mass spectrometry.2010

    • Author(s)
      Yu, Z., Chen, L.C., Erra-Balsells, R., Nonami, H., Hiraoka, K.
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 24 (11) Issue: 11 Pages: 1507-1513

    • DOI

      10.1002/rcm.4556

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 基礎から学ぶマススペクトロメトリ-/質量分析の源流 第8回 エレクトロスプレーの基礎(Fundamentals of Mass Spectrometry-Fundamentals of Electrospray-)2010

    • Author(s)
      平岡賢三
    • Journal Title

      J. Mass Spectrom. Soc. Jpn.

      Volume: Vol.58 (No.4) Pages: 139-154

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 探針エレクトロスプレーの開発と応用2010

    • Author(s)
      平岡賢三
    • Journal Title

      BUNSEKI KAGAKU

      Volume: Vol.59 (No.2) Pages: 95-105

    • NAID

      10025981050

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ethylenediaminetetraacetic Acid (EDTA) as an Auxiliary Tool in the Electrospray Ionization Mass Spectrometry Analysis of Native and Derivatized b-Cyclodextrins, Maltoses, and Fructans Contaminated with Ca and/or Mg.2010

    • Author(s)
      Silvana L. Giudicessi, M. Kaniz Fatema, Hiroshi Nonami, and Rosa Erra-Balsells
    • Journal Title

      Journal of the American Society for Mass Spectrometry

      Volume: 21 (9) Issue: 9 Pages: 1526-1529

    • DOI

      10.1016/j.jasms.2010.05.002

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lipase-catalyzed synthesis and characterization of a novel linear polyamidoamine oligomer.2010

    • Author(s)
      Leandro N. Monsalve, M. Kaniz Fatema, Hiroshi Nonami, Rosa Erra-Balsells, Alicia Baldessari
    • Journal Title

      Polymer

      Volume: 51 (14) Issue: 14 Pages: 1998-3005

    • DOI

      10.1016/j.polymer.2010.04.071

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diamond, Titanium Dioxide, Titanium Silicon Oxide, and Barium Strontium Titanium Oxide Nanoparticles as Matrixes for Direct Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry Analysis of Carbohydrates in Plant Tissues.2010

    • Author(s)
      Yousef Gholipour, Silvana L. Giudicessi, Hiroshi Nonami, Rosa Erra-Balsells
    • Journal Title

      Analytical Chemistry 82

      Pages: 5518-5526

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Real-time reaction monitoring by probe electrospray ionization mass spectrometry.2010

    • Author(s)
      Yu, Z., Chen, L. C., Erra-Balsells, R., Nonami, H., Hiraoka, K.
    • Journal Title

      Rapid Communications in Mass Spectrometry 24(11)

      Pages: 1507-1513

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Diamond, Titanium Dioxide, Titanium Silicon Oxide, and Barium Strontium Titanium Oxide Nanoparticles as Matrixes for Direct Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis of Carbohydrates in Plant Tissues.2010

    • Author(s)
      Yousef Gholipour, Silvana L.Giudicessi, Hiroshi Nonami, Rosa Erra-Balsells
    • Journal Title

      Analytical Chemistry

      Volume: 82 Pages: 5518-5526

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ethylenediaminetetraacetic Acid (EDTA) as an Auxiliary Tool in the Electrospray Ionization Mass Spectrometry Analysis of Native and Derivatized β-Cyclodextrins, Maltoses, and Fructans Contaminated with Ca and/or Mg.2010

    • Author(s)
      Silvana L.Giudicessi, M.Kaniz Fatema, Hiroshi Nonami, Rosa Erra-Balsells
    • Journal Title

      Journal of the American Society for Mass Spectrometry

      Volume: 21 Pages: 1526-1529

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipase-catalyzed synthesis and characterization of a novel linear polyamidoamine oligomer.2010

    • Author(s)
      Leandro N.Monsalve, M.Kaniz Fatema, Hiroshi Nonami, Rosa Erra-Balsells, Alicia Baldessari
    • Journal Title

      Polymer

      Volume: 51 Pages: 2998-3005

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of oligosaccharides and oligosaccharide alditols obtained by hydrolysis of agaroses and carrageenans, two important types of red seaweed polysaccharides.2010

    • Author(s)
      Fatema, M.K., Nonami, H., Ducatti, D.R.B., Goncalves, A.G., Duarte, M.E.R., Noseda, M.D., Cerezo, A.S., Erra-Balsells, R., Matulewicz, M.C.
    • Journal Title

      Carbohydrate Research

      Volume: 345 Pages: 275-283

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of water flow from the xylem on the growth-induced water potential and the growth-effective turgor associated with enlarging tomato fruit.2010

    • Author(s)
      Md.Mokter HOSSAIN, Hiroshi NONAM
    • Journal Title

      Environ.Control Biol.

      Volume: 48 Pages: 101-116

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Superposition of the transpiration-induced water potential and the growth-induced water potential associated with expanding tomato leaves.2010

    • Author(s)
      Hiroshi NONAMI, Md.Mokter HOSSAIN
    • Journal Title

      Environ.Control Biol.

      Volume: 48 Pages: 117-125

    • NAID

      10026721351

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of biomolecules from solutions with high concentration of salts using probe electrospray and nano-electrospray ionization mass spectrometry.2010

    • Author(s)
      Mridul Kanti Mandal, Lee Chuin Chen, Yutaka Hashimoto, Zhan Yu, Kenzo Hiraoka
    • Journal Title

      Anal.Methods

      Volume: 2 Pages: 1905-1912

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 基礎から学ぶマススペクトロメトリー/質量分析の源流 第8回 エレクトロスプレーの基礎2010

    • Author(s)
      平岡賢三
    • Journal Title

      J.Mass Spectrom.Soc.Jpn.

      Volume: 58 Pages: 139-154

    • NAID

      10026518064

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry.2009

    • Author(s)
      Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., Hiraoka, K
    • Journal Title

      Journal of the American Society for Mass Spectrometry

      Volume: 20 (12) Issue: 12 Pages: 2304-2311

    • DOI

      10.1016/j.jasms.2009.08.023

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of temperature on the stability of compounds used as UV-MALDI-MS matrix: 2,5-dihydroxybenzoic acid, 2,4,6-trihydroxyacetophenone, α-cyano-4-hydroxycinnamic acid, 3,5-dimethoxy-4-hydroxycinnamic acid, nor-harmane and harmane2009

    • Author(s)
      Olga I. Tarzi, Hiroshi Nonami and Rosa Erra-Balsells
    • Journal Title

      J. Mass. Spectrom.

      Volume: 44 Issue: 2 Pages: 260-277

    • DOI

      10.1002/jms.1506

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct Profiling of Phytochemicals in Tulip Tissues and in Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry.2009

    • Author(s)
      Yu, Z., Chen, L. C., Suzuki, H., Ari yada, 0., Erra-Balsells, R., Nonami, H., Hiraoka, K.
    • Journal Title

      Journal of the American Society for Mass Spectrometry 20(12)

      Pages: 2304-2311

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry2009

    • Author(s)
      Yu, Z.
    • Journal Title

      J.Am.Soc.Mass.Spectrom. 20

      Pages: 2304-2311

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of Probe Electrospray Ionization for Biological Sample Measurements2009

    • Author(s)
      Chen, L.C.
    • Journal Title

      Environ.Control Biol. 47

      Pages: 73-86

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of temperature on the stability of compounds used as UV-MALDI-MS matrix : 2, 5-dihydroxybenzoic acid, 2, 4, 6-trihydroxyacetophenone, α-cyano-4-hydroxycinnamicacid, 3, 5-dimethoxy-4-hydroxycinnamic acid, nor-harmane and harmane2009

    • Author(s)
      Olga I. Tarzi
    • Journal Title

      J. Mass. Spectrom. 44

      Pages: 260-277

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of Pressure Probe andUV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract.2008

    • Author(s)
      Yousef Gholipour, Hiroshi Nonami, and Rosa Erra-Balsells
    • Journal Title

      J Am Soc Mass Spectrom

      Volume: 19 Issue: 12 Pages: 1841-1848

    • DOI

      10.1016/j.jasms.2008.08.006

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In situ analysis of plant tissue underivatized carbohydrates and on-probeenzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix2008

    • Author(s)
      Yousef Gholipour, Hiroshi Nonami, Rosa Erra-Balsells
    • Journal Title

      Analytical Biochemistry

      Volume: 383 Issue: 2 Pages: 159-167

    • DOI

      10.1016/j.ab.2008.08.034

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract.2008

    • Author(s)
      Yousef Gholipour, Hiroshi Nonami, Rosa Erra-Balsells
    • Journal Title

      J Am Soc Mass Spectrom 19

      Pages: 1841-1848

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix.2008

    • Author(s)
      Yousef Gholipour, Hiroshi Nonami, Rosa Erra-Balsells
    • Journal Title

      Analytical Biochemistry 383

      Pages: 159-167

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Application of Pressure Probe and UV-MALDI-TOF MS for Direct Analysis of Plant Underivatized Carbohydrates in Subpicoliter Single-Cell Cytoplasm Extract2008

    • Author(s)
      Yousef Gholipour
    • Journal Title

      J. Am. Soc. Mass. Spectrom. 19

      Pages: 1841-1848

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix2008

    • Author(s)
      Yousef Gholipour
    • Journal Title

      Analytical Biochemistry 383

      Pages: 159-167

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細胞水分生理計測と質量分析を組み合わせたスピーキング・セル・アプローチ2011

    • Author(s)
      野並浩
    • Organizer
      日本生物環境工学会2011年札幌大会(オーガナイズドセッション招待講演)講演要旨:OS25:365-370.
    • Place of Presentation
      北海道大学,札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] In situ Monitoring of Pesticide Residues on the Surface of Tomato Fruit with Ultraviolet Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.2011

    • Author(s)
      Hiroshi Nonami, Rosa Erra-Balsells, Yousef Gholipour
    • Organizer
      The CIGR International Symposium WEF 2011
    • Place of Presentation
      Tower Hall Funabashi, Tokyo, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Nanoparticles as Matrices for Direct UV-MALDI-MS Analysis of Neutral Carbohydrates in Plant Tissues2011

    • Author(s)
      Erra-Balsells, R., Gholipour, Y. Giudicess, S.L., Nonami, H
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      Kyoto International Congress Center
    • Related Report
      2011 Final Research Report
  • [Presentation] Direct Tissue and Cell Analysis of Tulip Plants by Combining Pressure Probe and UV-MALDI-MS.2010

    • Author(s)
      Nonami, H., Gholipour, Y., Erra-Balsells, R
    • Organizer
      28th International Horticultural Congress. Lisboa Congress Centre
    • Place of Presentation
      Lisbon, Portugal
    • Related Report
      2011 Final Research Report
  • [Presentation] Speaking Plant Approach Combining Pressure Probe and MALDI Mass Spectrometry.2010

    • Author(s)
      Rosa ERRA-BALSELLS, Hiroshi NONAMI, Yousef GHOLIPOUR
    • Organizer
      The 58th Annual Conference on Mass Spectrometry & 1st Asian and Oceanic Mastrometry Conference
    • Place of Presentation
      Tsukuba, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Nor-harmane and b-carbolines as UV-MALDI matrices : the story of a bridge between photochemistry and UV-MALDI-MS2009

    • Author(s)
      Rosa ERRA-BALSELLS
    • Organizer
      日本質量分析学会
    • Place of Presentation
      つくば
    • Year and Date
      2009-05-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Application of probe electrospray ionization for biological sample measurements2008

    • Author(s)
      平岡賢三
    • Organizer
      日本生物環境工学会
    • Place of Presentation
      松山市 愛媛大学
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report
  • [Book] 質量分析の源流 - 基礎から学ぶマススペクトロメトリー2011

    • Author(s)
      平岡賢三
    • Total Pages
      243
    • Publisher
      株式会社国際文献印刷社
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://web.agr.ehime-u.ac.jp/%7Epbb/newpage5.html

    • Related Report
      2010 Self-evaluation Report
  • [Remarks]

    • URL

      http://web.agr.ehime-u.ac.jp/%7Epbb/newpage5.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2019-07-29  

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