| Budget Amount *help |
¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2008: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
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| Research Abstract |
This project has challenged to measurement and control of multi-scale spatiotemporal neuronal dynamics, three-figure spatially and ten-figure temporally. The aim of the project is as follows ; long term culture on multi-electrode array, detection of impulses, observation of way of growing neuronal networks, and analysis of obtained data. As a result, we established long term primary neuronal dissociative cell culture on multi-electrode arrays. Moreover, immunocytochemistry of some proteins was also established for main parts of neuron during several month. The main subject of construction of experimental systems to attack basic problems in neuroscience was achieved. Using constructed system, we obtained important issues of the related field. One of the results is a minimum density of surviving neurons that caused synchronized bursts. Further development of the present works is expected to execute the future fruitful studies on spatiotemporal neuronal dynamics.
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