Molecular mechanisms of structural plasticity of dendritic spine

• Project/Area Number: 20240032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HAYASHI Yasunori 独立行政法人理化学研究所, 記憶メカニズム研究チーム, チームリーダー (90466037)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥51,220,000 (Direct Cost: ¥39,400,000、Indirect Cost: ¥11,820,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
• Keywords: 細胞・組織 / シグナル伝達 / 神経科学 / 生理学 / 脳・神経 / 記憶学習 / シナプス可塑性 / 樹状突起スパイン / CaMKII / 細胞骨格 / 海馬 / 記憶・学習 / 海馬長期増強現象 / カルシウムカルモジュリン依存性蛋白質リン酸化酵素 / アクチン / コフィリン / 樹上突起棘 / カルシウム / カルモジュリン依存性蛋白質リン酸化酵素(CaMKII) / 海馬スライス / 二光子レーザー顕微鏡 / 長期増強現象 / CaMKll / 細胞情報伝達

Research Abstract

We found autophosphorylation of CaMKII on multiple serine and threonine residues on F-actin binding domain detaches CaMKII from F-actin. We concluded that CaMKII bundles F-actin under basal neuronal activity but when it is activated, it releases F-actin, thereby allowing its modification. This results in structural plasticity. In this way, we speculate that CaMKII act as a gate of structural plasticity.

Research Products

• [Journal Article] A rapid and effective FISH protocol with hybridization-sensitive fluorescent linear oligo probes (2012)
  • Author(s): Wang, D.O., Matsuno, H., Ikeda, S., Nakamura, A., Yanagisawa, H., Hayashi, Y., Okamoto, A
  • Journal Title: RNA Volume: 18 Pages: 166-175
• [Journal Article] Catching the engram: strategies to examine the memory trace. (2012)
  • Author(s): Sakaguchi M
  • Journal Title: Mol Brain. Volume: Sep 21 Issue: 1 Pages: 359-359
  • DOI: 10.1186/1756-6606-5-32
  • Peer Reviewed
• [Journal Article] The Ca2+ and Rho GTPase signaling pathways underlying activity-dependent actin remodeling at dendritic spines. (2012)
  • Author(s): Saneyoshi T
  • Journal Title: Cytoskeleton (Hoboken) Volume: Aug;69(8) Issue: 8 Pages: 545-54
  • DOI: 10.1002/cm.21037
  • Peer Reviewed
• [Journal Article] Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo (2011)
  • Author(s): Mower, A. F., Kwok, S. M., Yu, H., Majewska, A. K., Okamoto, K.-I., Hayashi, Y.*, Sur, M.* (*: co-corresponding authors)
  • Journal Title: Proc. Natl. Acad. Sci Volume: 108 Pages: 21241-21246
• [Journal Article] Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo (2011)
  • Author(s): Mower, A.F., Kwok, S.M., Yu, H., Majewska, A.K., Okamoto, K.-I., Hayashi, Y.*, Sur, M.* (*: co-corresponding authors)
  • Journal Title: Proc.Natl.Acad.Sci. Volume: 108 Issue: 52 Pages: 21241-21246
  • DOI: 10.1073/pnas.1108261109
  • Peer Reviewed
• [Journal Article] Structural plasticity of dendritic spines (2011)
  • Author(s): Bosch, M., Hayashi, Y.
  • Journal Title: Curr.Opin.Neurobiol. Volume: (印刷中) Issue: 3 Pages: 383-388
  • DOI: 10.1016/j.conb.2011.09.002
  • Peer Reviewed
• [Journal Article] Introduction of green fluorescent protein(GFP)into hippocampal neurons through viral infection (2010)
  • Author(s): Malinow R., Hayashi, Y., Maletic-Savatic M., Zaman S.H., Poncer J.-C., Shi S.-H., Esteban J.A., Osten P., Seidenman K
  • Journal Title: Cold Spring Harbor Protoc Volume: doi:10.1101/pdb.prot5406(online publication only)
• [Journal Article] The postsynaptic density proteins Homer and Shank form a polymeric network structure (2009)
  • Author(s): Hayashi, M.K., Tang, C., Verpelli, C., Narayanan, R., Stearns, M.H., Xu, R.-M., Li, H., Sala, C., Hayashi, Y
  • Journal Title: Cell Volume: 137 Pages: 159-171
• [Journal Article] The postsynaptic density proteins Homer and Shank form a polymeric network structure. (2009)
  • Author(s): Hayashi, M.K., Tang, C., Verpelli, C., Narayanan, R., Stearns, M.H., Xu, R.-M., Li, H., Sala, C., Hayashi, Y.
  • Journal Title: Cell 137 Pages: 159-171
  • URL: http://glutamate.brain.riken.jp/dokuwiki/hayashi_cell.pdf
  • Peer Reviewed
• [Journal Article] Functional dynamics of polo-like kinase-1 at the centrosome. (2009)
  • Author(s): Kishi, K., van Vugt, M.A., Okamoto, K.-I., Hayashi, Y., Yaffe, M.B.
  • Journal Title: Mol Cell Biol. 29 Pages: 3134-3150
  • URL: http://glutamate.brain.riken.jp/dokuwiki/kishi_mol_cell_biol.pdf
  • Peer Reviewed
• [Journal Article] The postsynaptic density proteins Homer and Shank form a polymeric network structure (2009)
  • Author(s): Hayashi, M.K., Tang, C., Verpelli, C., Narayanan, R., Stearns, M.H., Xu, R.-M., Li, H., Sala, C., Hayashi, Y.
  • Journal Title: Cell 137 Pages: 159-171
  • Peer Reviewed
• [Journal Article] Functional dynamics of polo-like kinase-1 at the centrosome (2009)
  • Author(s): Kishi, K., van Vugt, M.A., Okamoto, K.-I., Hayashi, Y., Yaffe, M.B.
  • Journal Title: Mol Cell Biol. 29 Pages: 3134-3150
  • Peer Reviewed
• [Journal Article] The roles of CaMKII and F-actin in the structural plasticity of dendritic spinesa potential molecular identity of a synaptic tag? (2009)
  • Author(s): Okamoto, K., Bosch, M., Hayashi, Y.
  • Journal Title: Physiology 24 Pages: 357-366
  • Peer Reviewed
• [Journal Article] Long-term potentiation : two pathways meet at neurogranin (2009)
  • Author(s): Hayashi, Y.
  • Journal Title: EMBO J 28 Pages: 3027-3039
• [Journal Article] The postsynaptic density proteins Homer and Shank from a polymeric network structure (2009)
  • Author(s): Hayashi, M. K.
  • Journal Title: Cell
  • Peer Reviewed
• [Presentation] CaMKII is a gating mechanism of activity-induced structural modification of hippocampal dendritic spines (2013)
  • Author(s): Hayashi Y
  • Organizer: Winter Conference on Brain Science, Breckenridge
  • Place of Presentation: Colorado, USA Jan
  • Year and Date: 2013-05-02
• [Presentation] A virtual navigation system for two-photon calcium imaging in mice (2012)
  • Author(s): Masaaki Sato, Tanvir Islam, Takashi Takekawa, Hiroshi Yamakawa, Masako Kawano, Yoko Yamaguchi, Tomoki Fukai, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Molecular mechanism of hippocampal learning and memory (2012)
  • Author(s): Hayashi Y
  • Organizer: 3rd LIN Symposium, Translating synaptic activity into neuronal plasticity
  • Place of Presentation: Tangermunde, Germany
• [Presentation] CaMKII serves as a gate of actin-mediated structural plasticity of dendritic spine (2012)
  • Author(s): Karam Kim, Gurpreet Lakhanpal, Akio Suzuki, Mariko Hayashi, Radhakrishnan Narayanan, Tomoki Matsuda, Takeharu Nagai, Kenichi Okamoto, Yasunori Hayashi
  • Organizer: Gordon Research Conference Synaptic Transmission
  • Place of Presentation: Waterville Valley, USA
• [Presentation] Molecular mechanism of hippocampal learning and memory (2012)
  • Author(s): Hayashi Y
  • Organizer: The 7th International Conference for Neurons and Brain Disease (Association for the Study of Neurons and Diseases)
  • Place of Presentation: Montreal, Canada
• [Presentation] Molecular mechanism of hippocampal learning and memory (2012)
  • Author(s): Hayashi Y
  • Organizer: 2012 International Brain Research Symposium
  • Place of Presentation: Daegu, Korea
• [Presentation] Molecular mechanism of hippocampal synaptic plasticity (2012)
  • Author(s): Y Hayashi
  • Organizer: 3rd LIN Symposium, Translating synaptic activity into neuronal plasticity
  • Place of Presentation: Tangermunde, Germany
  • Invited
• [Presentation] Structural roles of CaMKII (2011)
  • Author(s): Hayashi, Y.
  • Organizer: 日本神経化学会
  • Place of Presentation: 金沢(招待講演)
  • Year and Date: 2011-09-26
• [Presentation] A quantitative anyalysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] The regulation of dendritic spine structural plasticity by CaMKII beta (2011)
  • Author(s): Lakhanpal, G.K., Kim, K., Kato-Hayashi, M., Hayashi, Y., Okamoto, K
  • Organizer: 8^<th> IBRO World Congress of Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-15
• [Presentation] Phosphoisotype analysis of AMPA receptor GluR1 (2011)
  • Author(s): Yasunori Hayashi
  • Organizer: Gordon Research Conference Excitatory Synapses & Brain Function
  • Place of Presentation: Easton, MA, USA(招待講演)
  • Year and Date: 2011-06-29
• [Presentation] A quantitative anyalysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y
  • Organizer: SfN 2011
  • Place of Presentation: Washington DC, USA
• [Presentation] The role of CaMKII beta in modulating dendritic spine structural plasticity (2011)
  • Author(s): Lakhanpal, G., Kim, K., Kato-Hayashi, M., Hayashi, Y., Okamoto, K
  • Organizer: SfN 2011
  • Place of Presentation: Washington DC, USA
• [Presentation] CaMKII gates rapid structural plasticity in hippocampal dendritic spines (2011)
  • Author(s): Kim, K., Hayashi, M., Narayanan, R., Suzuki, A., Matsuura, K., Okamoto, K., Hayashi, Y
  • Organizer: SfN 2011
  • Place of Presentation: Washington DC, USA
• [Presentation] Principle and application of fluorescence microscopy in neuroscience (2011)
  • Author(s): Hayashi, Y
  • Organizer: IBRO School
  • Place of Presentation: Beijing, China
• [Presentation] A quantitative analysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] In vivo two-photon imaging of neuronal circuit activity using transgenic mice that express fluorescent calcium sensor proteins in the brain (2011)
  • Author(s): Sato, M., Kawano, M., Islam, T., Yamaguchi, Y., Ohkura, M., Nakai, J., Hayashi, Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Phosphoisotype analysis of AMPA receptor GluR1 (2011)
  • Author(s): Hayashi Y
  • Organizer: Gordon Research Conference Excitatory Synapses & Brain Function
  • Place of Presentation: Easton, MA, USA
• [Presentation] The regulation of dendritic spine structural plasticity by CaMKII beta (2011)
  • Author(s): Hayashi Y
  • Organizer: 8th IBRO World Congress of Neuroscience
  • Place of Presentation: Florence, Italy
• [Presentation] Structural roles of CaMKII (2011)
  • Author(s): Hayashi Y
  • Organizer: 第54回日本神経化学会
  • Place of Presentation: 金沢
• [Presentation] Temporal progression of the structural and molecular remodeling of dendritic spines after LTP induction (2011)
  • Author(s): Hayashi Y
  • Organizer: Neuronal Communication Beyond the Synaptic Cleft - International conference and workshop
  • Place of Presentation: Niigata
• [Presentation] Regulation of structural function of CaMKII by multiple autophosphorylation sites (2010)
  • Author(s): Yasunori Hayashi
  • Organizer: 7^<th> FENS Forum of European Neuroscience
  • Place of Presentation: Amsterdam, オランダ
  • Year and Date: 2010-07-05
• [Presentation] Sequential reorganization of synaptic components after LTP induction (2010)
  • Author(s): Hayashi Y
  • Organizer: Gordon Research Conferences Synaptic Transmission
  • Place of Presentation: Biddeford, ME, USA
• [Presentation] Regulation of structural function of CaMKII by multiple autophosphorylation sites (2010)
  • Author(s): Hayashi Y
  • Organizer: 7^(th) FENS Forum of European Neuroscience
  • Place of Presentation: Amsterdam
• [Presentation] Molecular mechanism of hippocampal synaptic plasticity (2010)
  • Author(s): Hayashi Y
  • Organizer: Bristol 2010 From molecules to neuronal
  • Place of Presentation: Bristol, UK
• [Presentation] Sequential reorganization of synaptic components after LTP induction. (2010)
  • Author(s): Hayashi, Y.
  • Organizer: Gordon Research Conferences Synaptic Transmission
  • Place of Presentation: Biddeford, ME, USA.
• [Presentation] Regulation of structural function of CaMKII by multiple autophosphorylation sites. (2010)
  • Author(s): Hayashi, Y., Karam K., Hayashi M., Matsuura K., Narayanan R., Lakhanpal G., Okamoto K.
  • Organizer: 7th FENS Forum of European Neuroscience
  • Place of Presentation: Amsterdam, Nederland.
• [Presentation] Ultrastructural reorganization after long term potentiation of a single dendritic spine (2009)
  • Author(s): J.Castro, M.Bosch, Y.Hayashi, M.Sur
  • Organizer: Annual meeting of Society for Neuroscience
  • Place of Presentation: Chicago, Illinois, USA
  • Year and Date: 2009-10-18
• [Presentation] Structural and molecular reorganization of a single dendritic spine during long-term potentiation (2009)
  • Author(s): M.Bosch, J.Castro, R.Narayanan, M.Sur, Y.Hayashi
  • Organizer: Annual meeting of Society for Neuroscience
  • Place of Presentation: Chicago, Illinois, USA
  • Year and Date: 2009-10-18
• [Presentation] Eye domain-specific synaptic CaMKII activation during ocular dominance plasticity in vivo (2009)
  • Author(s): Y.Hayashi, A.F.Mower, S.Kwok, H.Yu, A.Majewska, K.Okamoto, M.Sur
  • Organizer: Annual meeting of Society for Neuroscience
  • Place of Presentation: Chicago, Illinois, USA
  • Year and Date: 2009-10-18
• [Presentation] Molecular mechanism of hippocampal synaptic plasticity (2009)
  • Author(s): Hayashi, Y.
  • Organizer: Frontiers in Neurophotonics 2009
  • Place of Presentation: Ville de Quebec, Quebec, Canada
  • Year and Date: 2009-06-08
• [Presentation] Genetically encoded probe for fluorescence lifetime imaging of CaMKII activity (2008)
  • Author(s): 郭小敏, ら
  • Organizer: Neuroscience Meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2008-11-19
• [Presentation] Dynamics of CaMKIIα activation induced by changes in physiological activity in visual cortex (2008)
  • Author(s): Amanda Mower, ら
  • Organizer: Neuroscience Meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2008-11-19
• [Presentation] Structural framework of the postsynaptic density formed by a high-order complex between Homer and Shank (2008)
  • Author(s): 林真理子, ら
  • Organizer: Neuroscience Meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2008-11-18
• [Presentation] Single dendritic spine two-photon glutamate uncaging reveals the diversity of postsynaptic protein dynamics during long-term potentiation (2008)
  • Author(s): M. BOSCH
  • Organizer: Neuroscience Meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2008-11-17
• [Presentation] A virtual navigation system for two-photon calcium imaging in mice
  • Author(s): Masaaki Sato
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋
• [Book] Catching the engram: strategies to examine the memory trace (2012)
  • Author(s): Sakaguchi M, Hayashi Y
  • Publisher: Mol Brain
• [Book] The Ca2+ and Rho GTPase signaling pathways underlying activity-dependent actin remodeling at dendritic spines (2012)
  • Author(s): Saneyoshi T, Hayashi Y
  • Publisher: Cytoskeleton (Hoboken)
• [Book] Roles of neuronal activity-induced gene products in Hebbian and homeostatic synaptic plasticity, tagging, and capture (2012)
  • Author(s): Hayashi Y, Okamoto K, Bosch M, Futai K
  • Publisher: Adv Exp Med Biol
• [Book] Structural plasticity of dendritic spines (2012)
  • Author(s): Bosch M, Hayashi Y
  • Publisher: Curr Opin Neurobiol
• [Book] Introduction of green fluorescent protein (GFP) into hippocampal neurons through viral infection. (2010)
  • Author(s): Malinow R., Hayashi, Y., Maletic-Savatic M., Zaman S.H., Poncer J.-C., Shi S.-H., Esteban J.A., Osten P., Seidenman K.
  • Total Pages: 5406
  • Publisher: In Cold Spring Harbor Protoc.
• [Book] Synaptic plasticity in health and disease (2010)
  • Author(s): Hayashi, Y., Okamoto, K., Bosch, M., Futai K.
  • Publisher: Springer Verlag(In press)
• [Book] Transsynaptic regulation of presynaptic release machinery in central synapses by cell adhesion molecules, in Molecular Mechanisms of Neurotransmitter Release, (edited by Wang) (2008)
  • Author(s): 二井健介, 林康紀
  • Total Pages: 352
  • Publisher: Humana Press
• [Book] Neuronal activity-induced genes and synaptic plasticity, tagging and capture. In Synaptic plasticity in health and disease (ed. Kreutzer and Sala, Springer Verlag, in the press)
  • Author(s): Hayashi, Y., Okamoto, K., Bosch, M., Futai K.
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