| Project/Area Number |
20241011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Tohoku University |
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Principal Investigator |
YASUI Akira 東北大学, 加齢医学研究所, 教授 (60191110)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Satoshi 東北大学, 加齢医学研究所, 助教 (00375114)
KANNO Shinichirou 東北大学, 加齢医学研究所, 講師 (10400417)
TANAKA Kouzo 東北大学, 加齢医学研究所, 准教授 (00304452)
TAKAO Masashi 東北大学, 加齢医学研究所, 講師 (70216612)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2010: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2009: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2008: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Keywords | ポリADPリボース / DNA修復 / 活性酸素 / 細胞老化 / 癌化 |
|---|
| Research Abstract |
Using laser micro-irradiation of living human cell and identification of protein complexes recruited to DNA damaged site by proteomics, we have analyzed and identified DNA damage response of proteins within human cell. For DNA single-strand breaks we have identified two novel human proteins with DNA end-and exo-nuclease activities, which activate poly(ADP-ribose polymerase 1 (PARP1)by creating gap at a nick in DNA. For DNA double-strand breaks (DSB)we identified an ATP-dependent chromatin remodeling complex belonging to ISWI family, CHRAC, which is required for the loading of KU complex at DSB. Especially, ACF1 becomes bound to KU70 more tightly by treatment of cells with chemical agents creating DSB and initiates non-homologous end joining of DSB. Thus, we showed that chromatin remodeling is essential for the initiation of repair of DNA strand breaks.
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