Analysis of DNA damage response using gene-disrupted DT40 clones.

• Project/Area Number: 20241012
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: TAKEDA Shunichi Kyoto University, 大学院・医学研究科, 教授 (60188191)
• Co-Investigator(Kenkyū-buntansha): MOTEGI Akira 京都大学, 大学院・医学研究科, 助教 (80452332)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥49,920,000 (Direct Cost: ¥38,400,000、Indirect Cost: ¥11,520,000); Fiscal Year 2010: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000); Fiscal Year 2009: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2008: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
• Keywords: DNA組換え / 標的組換え / 遺伝子治療 / DT40 / ニワトリBリンパ細胞 / DNA組み換え / 標的組み換え / DNA修復 / 変異 / 化審法 / 有害化学物質 / ハイスループットスクリーニング

Research Abstract

Homologous recombination (HR) plays a major role in repairing DNA damage caused by chemotherapeutic agents, such as camptothecin and cisplatin. HR is carried out by coordinate actions of more than 50 different proteins. We have analyzed the role of the individual HR proteins by disrupting genes encoding the HR proteins in the chicken DT40 cells. We have revealed the function of the following HR proteins, including CtIP, DNA polymerases θ and ν, Fan1, Palb-2, Sfr1, Slx4, and Sws1.

Research Products

• [Journal Article] Collaborative action of Brca1 and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair. (2010)
  • Author(s): Nakamura K, Kogame T, Oshiumi H, Shinohara A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S, Taniguchi Y.
  • Journal Title: PLoS Genet. 6
• [Journal Article] PTIP promotes DNA double-strand break repair through homologous recombination. (2010)
  • Author(s): Wang X, Takenaka K, Takeda S.
  • Journal Title: Genes Cells 15 Pages: 243-54
• [Journal Article] RAP80 acts independently of BRCA1 in repair of topoisomerase II poison-induced DNA damage. (2010)
  • Author(s): Iijima J, Zeng Z, Takeda S, Taniguchi Y.
  • Journal Title: Cancer Res. 70 Pages: 8467-74
• [Journal Article] DNA polymerases ν and θ are required for efficient Immunoglobulin V gene diversification in chicken. (2010)
  • Author(s): Kohzaki M, Nishihara K, Hirota K, Sonoda E, Yoshimura M, Ekino S, Butler JE, Watanabe M, Halazonetis T, Takeda S.
  • Journal Title: J Cell Biol. 189 Pages: 1117-27
• [Journal Article] Simultaneous disruption of two DNA polymerases, Polη and Polζ, in avian DT40 cells unmasks the role of Polη in cellular response to various DNA lesions. (2010)
  • Author(s): Hirota K, Sonoda E, Kawamoto T, Motegi A, Masutani C, Hanaoka F, Szuts D, Iwai S, Sale JE, Lehmann A, Takeda S.
  • Journal Title: PLoS Genet. 6
  • NAID: 120002661519
• [Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination. (2010)
  • Author(s): Takizawa Y, Qing Y, Takaku M, Ishida T, Morozumi Y, Tsujita T, Kogame T, Hirota K, Takahashi M, Shibata T, Kurumizaka H, Takeda S.
  • Journal Title: Nucleic Acids Res. 38 Pages: 5059-74
• [Journal Article] KIAA1018/FAN1 nuclease protects cells against genomic instability induced by interstrand cross-linking agents. (2010)
  • Author(s): Yoshikiyo K, Kratz K, Hirota K, Nishihara K, Takata M, Kurumizaka H, Horimoto S, Takeda S, Jiricny J.
  • Journal Title: Proc Natl Acad Sci USA. 107 Pages: 21553-7
• [Journal Article] RAP80 acts independently of BRCA1 in repair of topoisomerase II poison-induced DNA damage. (2010)
  • Author(s): Iijima J, Zeng Z, Takeda S, Taniguchi Y.
  • Journal Title: Cancer Res. Volume: 70 Pages: 8467-8474
  • Peer Reviewed
• [Journal Article] DNA polymerases ν and θ are required for efficient Immunoglobulin V gene diversification in chicken. (2010)
  • Author(s): Kohzaki M, Nishihara K, Hirota K, Sonoda E, Yoshimura M, Ekino S, Butler JE, Watanabe M, Halazonetis T, Takeda S.
  • Journal Title: J Cell Biol. Volume: 189 Pages: 1117-1127
  • Peer Reviewed
• [Journal Article] Simultaneous disruption of two DNA polymerases, Polη and Polζ, in avian DT40 cells unmasks the role of Polη in cellular response to various DNA lesions. (2010)
  • Author(s): Hirota K, Sonoda E, Kawamoto T, Motegi A, Masutani C, Hanaoka F, Szuts D, Iwai S, Sale JE, Lehmann A, Takeda S.
  • Journal Title: PLoS Genet. Volume: 6
  • NAID: 120002661519
  • Peer Reviewed
• [Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination. Nucleic Acids Res.38:5059-74. (2010)
  • Author(s): Takizawa Y, Qing Y, Takaku M, Ishida T, Morozumi Y, Tsujita T, Kogame T, Hirota K, Takahashi M, Shibata T, Kurumizaka H, Takeda S.
  • Journal Title: Nucleic Acids Res. Volume: 38 Pages: 5059-5074
  • Peer Reviewed
• [Journal Article] A novel approach using DNA-repair-deficient chicken DT40 cell lines for screening and characterizing the genotoxicity of environmental contaminants. Environ Health Perspect. (2010)
  • Author(s): Ji K, Kogame T, Choi K, Wang X, Lee J, Taniguchi Y, Takeda S.
  • Journal Title: Environ Health Perspect. VOL.117 Pages: 1737-1744
  • Peer Reviewed
• [Journal Article] Collaborative action of Brcal and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair. (2010)
  • Author(s): Nakamura K, Kogame T, Oshiumi H, Shinohare A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S, Taniguchi Y.
  • Journal Title: PLoS Genet. VOL.6
  • Peer Reviewed
• [Journal Article] Geneticevidence for single-strand lesions initiating Nbs1-dependent homologous recombination in diversification of IgV in chicken B lymphocytes. (2009)
  • Author(s): Nakahara M, Sonoda E, Nojima K, Sale JE, Takenaka K, Kikuchi K, Taniguchi Y, Nakamura K, Sumitomo Y, Bree RT, Lowndes NF, Takeda S.
  • Journal Title: PLoS Genet. 5
• [Journal Article] The vital link between the ubiquitin-proteasome pathway and DNA repair : Impact on cancer therapy. (2009)
  • Author(s): Motegi A, Murakawa Y, Takeda S.
  • Journal Title: Cancer Lett.(Review) 283 Pages: 1-9
• [Journal Article] Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences. (2009)
  • Author(s): Kikuchi K, Abdel-Aziz HI, Taniguchi Y, Yamazoe M, Takeda S, Hirota K.
  • Journal Title: J Biol Chem. 284 Pages: 26360-7
• [Journal Article] A novel approach using DNA-repair-deficient chicken DT40 cell lines for screening and characterizing the genotoxicity of environmental contaminants. (2009)
  • Author(s): Ji K, Kogame T, Choi K, Wang X, Lee J, Taniguchi Y, Takeda S.
  • Journal Title: Environ Health Perspect. 117 Pages: 1737-44
• [Journal Article] Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences. (2009)
  • Author(s): Kikuchi K, Abdel-Aziz HI, Taniguchi Y, Yamazoe M, Takeda S, Hirota K.
  • Journal Title: J Biol Chem. VOL.284 Pages: 26360-26367
  • Peer Reviewed
• [Journal Article] The vital link between the ubiquitin-proteasome pathway and DNA repair : Impact on cancer therapy. (2009)
  • Author(s): Motegi A, Murakawa Y, Takeda S.
  • Journal Title: Cancer Lett. VOL.283 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells. (2009)
  • Author(s): Kong X, Ball AR, Jr., Sonoda E, Feng J. Takeda S, Fukagawa T, Yen TJ, Yokomori K
  • Journal Title: Mol Cell Biol 20 Pages: 1289-1301
  • Peer Reviewed
• [Journal Article] The Vital Link between the Ubiquitin-Proteasome Pathway and DNA Repair : Impact on Cancer Therapy. (2009)
  • Author(s): Motegi A, Murakawa Y, Takeda S.
  • Journal Title: Cancer Lett
  • Peer Reviewed
• [Journal Article] Genetic evidence for single-strand lesions initiating Nbsl-dependent homologous recombination in diversification of Ig V in chicken B lymphocytes. (2009)
  • Author(s): Nakahara M, Sonoda E, Nojima K, Sale JE, Takenaka K, Kikuchi K, Taniguchi Y, Nakamura K, Sumlitomo Y, Bree RT, Lowndes NF, Takeda S.
  • Journal Title: PLoS Genet 5
  • Peer Reviewed
• [Journal Article] An ATM and ATR dependent checkpoint inactivates spindle assembly by targeting CEP63. (2009)
  • Author(s): Smith E, Dejsuphong D, Balestrini A, Hampel M, Lenz C, Vindigni A, Takeda S, Costanzo V.
  • Journal Title: Nat Cell Biol 11 Pages: 278-285
  • Peer Reviewed
• [Journal Article] Genetic Evidence That the Non-homologous End-joining Repair Pathway Is Involved in LINE Retrotransposition. (2009)
  • Author(s): Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N.
  • Journal Title: PLoS Genet
  • Peer Reviewed
• [Journal Article] DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion. (2008)
  • Author(s): Oka H, Sakai W, Sonoda E, Nakamura J, Asagoshi K, Wilson SH, Kobayashi M, Yamamoto K, Heierhorst J, Takeda S, Taniguchi Y.
  • Journal Title: Biochem Biophys Res Commun. 371 Pages: 225-9
• [Journal Article] The 9-1-1 DNA clamp is required for immunoglobulin gene conversion. (2008)
  • Author(s): Saberi A, Nakahara M, Sale JE, Kikuchi K, Arakawa H, Buerstedde J-M, Takeda S, Sonoda E.
  • Journal Title: Mol Cell Biol. 28 Pages: 6113-22
• [Journal Article] Cyclin-dependent kinases and cell-cycle transitions: does one fit all? (2008)
  • Author(s): Hochegger H, Takeda S, Hunt T.
  • Journal Title: Nat Rev Mol Cell Biol.(Review) 9 Pages: 910-6
• [Journal Article] Ku70 prevents genome instability resulting from heterozygosity of the telomei-ase RNA component in a vertebrate tumour line. (2008)
  • Author(s): Faure V, Wenner T, Cooley C, Bourke E, Farr CJ, Takeda S, Morrison CG
  • Journal Title: DNA Repair (Amst) 7 Pages: 713-724
  • Peer Reviewed
• [Journal Article] DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion. (2008)
  • Author(s): Oka H, Sakai W, Sonoda E, Nakamura J, Asagoshi K, Wilson SH, Kobaysashi M, Yamamoto K, Hcierhorst J, Takeda S, Taniguchi Y
  • Journal Title: Biochem Biophys Res Comraun 371 Pages: 225-229
  • Peer Reviewed
• [Journal Article] Single stranded DNA binding protein hSSB1 is critical for genomic stability. (2008)
  • Author(s): Richard D. Bolderson, E. Cubeddu L, Wadsworth RIN, Savage K, Sharma GG, Nicolette ML, Tsvetanov S. Mellwraith MJ, Pandita R, Takeda S, Hay TT, Gautier J, West SC, Paull TT, Pandital TK, White MF, Khanna KK
  • Journal Title: Nature 453 Pages: 677-681
  • Peer Reviewed
• [Journal Article] The 9-1-1 DNA clamp is required for immunoglobulin gene conversion. (2008)
  • Author(s): Saberi A, Nakahara M, Sale JE, Kikuchi K, Arakawa H, Buerstedde J-H, Takeda S, Sonoda E.
  • Journal Title: Mol Cell Biol 28 Pages: 6113-6122
  • Peer Reviewed
• [Journal Article] KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation. (2008)
  • Author(s): Abe T, Ishiai H, Hosnno Y. Yoshimura A, Tada S, Adachi N. Koyama H, Takata H, Takeda S. Enomoto T, Seki M
  • Journal Title: Cell Signal 20 Pages: 1978-1985
  • Peer Reviewed
• [Journal Article] PARP-1 ensures the modulation of replication fork progression by homologous recombination on damaged DNA. (2008)
  • Author(s): Sugimura K, Takebayashi S, Taguchi H, Takeda S, Okuraura K
  • Journal Title: J Cell Biol 183 Pages: 1203-1212
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinases and cell-cycle transitions : does one fit all? (2008)
  • Author(s): Hochegger H, Takeda S, Hunt T.
  • Journal Title: Nat Rev Mol Cell Biol (Review) 9 Pages: 910-916
  • Peer Reviewed
• [Journal Article] Involvement of SLX4 in interstrand cross-link repair is regulated by the Fanconi anemia pathway.
  • Author(s): Yamamoto KN, Kobayashi S, Tsuda M, Kurumizaka H, Takata M, Kono K, Jiricny J, Takeda S, Hirota K.
  • Journal Title: Proc Natl Acad Sci USA. in press
  • NAID: 120003072120
• [Journal Article] KIAA1018/FAN1 nuclease protects cells against genomic instability induced by interstrand cross-linking agents.
  • Author(s): Yoshikiyo K, Kratz K, Hirota K, Nishihara K, Takata M, Kurumizaka H, Horimoto S, Takeda S, Jiricny J.
  • Journal Title: Proc Natl Acad Sci USA. Volume: (in press)
  • Peer Reviewed
• [Journal Article] The involvement of SLX4 in interstrand cross-link repair is regulated by the Fanconi anemia pathway.
  • Author(s): Yamamoto KN, Kobayashi S, Tsuda M, Kurumizaka H, Takata M, Kono K, Jiricny J, Takeda S, Hirota K.
  • Journal Title: Proc Natl Acad Sci USA. Volume: (in press)
  • NAID: 120003072120
  • Peer Reviewed
• [Journal Article] PTIP promotes DNA double-strand break repair through homologous recombination.
  • Author(s): Wang X, Takenaka K, Takeda S
  • Journal Title: Genes Cells (in press)
  • Peer Reviewed
• [Presentation] SLX4 Links Fanconi Anemia Pathway to Structure Specific Endonucleases, MUS81, SLX1 and XPF in Interstrand Crosslink Repair. (2011)
  • Author(s): Takeda S.
  • Organizer: Keystone Symposia, Genomic Instability and DNA Repair
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2011-01-31
• [Presentation] SLX4 Links Fanconi Anemia Pathway to Structure Specific Endonucleases, MUS81, SLX1 and XPF in Interstrand Crosslink Repair (2011)
  • Author(s): 武田俊一
  • Organizer: Keystone Symposia, Genomic Instability and DNA Repair
  • Place of Presentation: Keystone, Colorado, USA
• [Presentation] Differential Functional interactions between the RNF8 and RAD18 E3 ubiquitin ligases in Translesion DNA Synthesis and DSB Repair. (2010)
  • Author(s): Takeda S.
  • Organizer: EMBO Workshop, The Interface between the Ubiquitin Family and the DNA Damage Response
  • Place of Presentation: Rovinj, Croatia
  • Year and Date: 2010-09-05
• [Presentation] Analysis of Immunoglobulin V Gene Diversification in the Chicken DT40 B Lymphocyte Line Reveals the Role of DNA Polymerases in Homologous Recombination and Translesion DNA Synthesis (2010)
  • Author(s): Takeda S
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: Nagasaki University, Nagasaki(長崎県)
  • Year and Date: 2010-02-19
• [Presentation] Analysis of Immunoglobulin V Gene Diversification in the Chicken DT40 B Lymphocyte Line Reveals the Role of DNA Polymerases in Homologous Recombination and Translesion DNA Synthesis (2010)
  • Author(s): 武田俊一
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010(招待講演)
  • Place of Presentation: Nagasaki University, Nagasaki
• [Presentation] Differential Functional interactions between the RNF8 and RAD18 E3 ubiquitin ligases in Translesion DNA Synthesis and DSB Repair (2010)
  • Author(s): 武田俊一
  • Organizer: EMBO Workshop, The Interface between the Ubiquitin Family and the DNA Damage Response
  • Place of Presentation: Rovinj, Croatia
• [Presentation] Chemical Genetic Method to Detect Environmental Mutagens and to Screen Anti-Malignant Chemical Compounds (2009)
  • Author(s): Takeda S
  • Organizer: The 40th International Symposium of the Princess Takamatsu Cancer Research Fund
  • Place of Presentation: Hotel Grand Palace(東京都)
  • Year and Date: 2009-11-11
• [Presentation] Systematic Reverse Genetic Study of DNA Damage Response, Using the Chicken DT40 Cells (2009)
  • Author(s): Takeda S
  • Organizer: Annual Meeting of the Korean Society for Molecular and Cellular Biology
  • Place of Presentation: COEX Center, Seoul(韓国)
  • Year and Date: 2009-10-16
• [Presentation] DNAを損傷するタイプの抗がん治療時に機能するDNA修復分子 (2009)
  • Author(s): 武田俊一
  • Organizer: 癌学会学術総会
  • Place of Presentation: パシフィコ横浜, 横浜, (神奈川県)
  • Year and Date: 2009-10-02
• [Presentation] Reverse Genetic Analysis of DNA polymerases in TLS (2009)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Colby-Sawyer College, New London, New Hampshire(アメリカ)
  • Year and Date: 2009-08-11
• [Presentation] Reverse Genetic Study of RAD51 mediators, BRCA1, BRCA2, RAD51 paralogs, Rad52, Sws1, and Sfr1 (2009)
  • Author(s): Takeda S
  • Organizer: FASEB Genetic Recombination and Genome Rerrangements
  • Place of Presentation: Snowmass Village, Colorado(アメリカ)
  • Year and Date: 2009-08-06
• [Presentation] A novel approach using DNA repair-deficient chicken DT40 cell lines for screening and characterizing the genotoxicity of chemical compounds and environmental contaminants (2009)
  • Author(s): Takeda S
  • Organizer: Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars sur Ollon(スイス)
  • Year and Date: 2009-05-16
• [Presentation] Regulation of DNA damage response by ubiquitin ligases (2009)
  • Author(s): Takeda S
  • Organizer: DNA Repair
  • Place of Presentation: クレタ島(ギリシャ)
  • Year and Date: 2009-04-22
• [Presentation] Analysis of Genome Maintenance Using Chemical Genetics (2009)
  • Author(s): 武田俊一
  • Organizer: International Symposium on Tumor Biology in Kanazawa 2009(金沢がん生物学国際シンポジウム2009)(招待講演)
  • Place of Presentation: 金沢,石川
  • Year and Date: 2009-02-19
• [Presentation] Analysis of Genome Maintenance Using Chemical Genetics (2009)
  • Author(s): 武田俊一
  • Organizer: 金沢がん生物学国際シンポジウム2009
  • Place of Presentation: 金沢(石川)
  • Year and Date: 2009-02-19
• [Presentation] A Critical Role for the Ubiquitin-Conjugating Enzymes Ubcl3 and RNF8 in Homologous Recombination (2009)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura (アメリカ)
  • Year and Date: 2009-02-10
• [Presentation] A Critical Role for the Ubiquitin-Conjugating Enzymes Ubc13 and RNF8 in Homologous Recombination (2009)
  • Author(s): 武田俊一
  • Organizer: Gordon Research Conference(Invited)
  • Place of Presentation: Ventura,USA
• [Presentation] Regulation of DNA damage response by ubiquitin ligases (2009)
  • Author(s): 武田俊一
  • Organizer: DNA Repair(Invited)
  • Place of Presentation: Crete, Greece
• [Presentation] A novel approach using DNA repair-deficient chicken DT40 cell lines for screening and characterizing the genotoxicity of chemical compounds and environmental contaminants (2009)
  • Author(s): 武田俊一
  • Organizer: Chromosome Dynamics and Genome Stability(Invited)
  • Place of Presentation: Villars sur Ollon, Switzerland
• [Presentation] Reverse Genetic Study of RAD51 mediators, BRCA1, BRCA2, RAD51 paralogs, RAD52, Sws1, and Sfr1 (2009)
  • Author(s): 武田俊一
  • Organizer: FASEB Genetic Recombination and Genome Rerrangements(Invited)
  • Place of Presentation: Snowmass Village, Colorado, USA
• [Presentation] Reverse Genetic Analysis of DNA polymerases in TLS (2009)
  • Author(s): 武田俊一
  • Organizer: Gordon Research Conference, Colby-Sawyer College(Invited)
  • Place of Presentation: New London, New Hampshire, USA
• [Presentation] DNAを損傷するタイプの抗がん治療時に機能するDNA修復分子 (2009)
  • Author(s): 武田俊一
  • Organizer: 第68回癌学会学術総会,パシフィコ横浜(招待講演)
  • Place of Presentation: 横浜,神奈川
• [Presentation] Systematic Reverse Genetic Study of DNA Damage Response, Using the Chicken DT40 Cells (2009)
  • Author(s): 武田俊一
  • Organizer: Annual Meeting of the Korean Society for Molecular and Cellular Biology(Invited)
  • Place of Presentation: COEX Center, Seoul, Korea
• [Presentation] Chemical Genetic Method to Detect Environmental Mutagens and to Screen Anti-Malignant Chemical Compounds (2009)
  • Author(s): 武田俊一
  • Organizer: The 40th International Symposium of the Princess Takamatsu Cancer Research Fund(Invited)
  • Place of Presentation: Hotel Grand Palace, Tokyo
• [Presentation] ニワトリByンパ細胞を使ったDNA損傷応答機構の遺伝学的解析 (2008)
  • Author(s): 武田俊一
  • Organizer: 放射線防護センターシンポジウム
  • Place of Presentation: 稲毛(千葉)
  • Year and Date: 2008-12-16
• [Presentation] A critical role for the ubiquitin-conjugating enzymes Ubc13 and RNF8 in homologous recombination (2008)
  • Author(s): 武田俊一
  • Organizer: 3R Symposium 2008(招待講演)
  • Place of Presentation: つま恋,静岡
  • Year and Date: 2008-10-30
• [Presentation] A critical role for the ubiquitin-conjugating enzymes Ubcl3 and RNF8 in homologous recombination (2008)
  • Author(s): 武田俊一
  • Organizer: 3R Symposium 2008
  • Place of Presentation: つま恋(静岡)
  • Year and Date: 2008-10-30
• [Presentation] A critical role for the ubiquitin-conjugating enzymes Ubc13 and RNF8 in homologous recombination (2008)
  • Author(s): Takeda S
  • Organizer: Genome Stability in Health and Disease
  • Place of Presentation: Tel Aviv (イスラエル)
  • Year and Date: 2008-09-23
• [Presentation] Collaboration of BRCA1 and CtIP in cellular tolerance to topoisomerase poisons (2008)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Oxford (イギリス)
  • Year and Date: 2008-07-22
• [Presentation] The role of anti-recombination in replication-fork collapse (2008)
  • Author(s): Takeda S
  • Organizer: Molecular and Clinical Mechanisms in Bloom's Syndomeand Related Diosorders
  • Place of Presentation: Chicago (アメリカ)
  • Year and Date: 2008-05-28
• [Presentation] Multiple functions of Mrell/Rad50/Nbs1 complex (2008)
  • Author(s): Takeda S
  • Organizer: 2008 EMBO Recombination Mechamisms
  • Place of Presentation: II Ciocco (イタリア)
  • Year and Date: 2008-05-20
• [Presentation] Multiple functions of Mre11/Rad50/Nbs1 complex (2008)
  • Author(s): 武田俊一
  • Organizer: 2008 EMBO Recombination Mechamisms(Invited)
  • Place of Presentation: Il Ciocco, Italy
• [Presentation] The role of antirecombination in replication-fork collapse (2008)
  • Author(s): 武田俊一
  • Organizer: Molecular and Clinical Mechanisms in Bloom's Syndome and Related Diosorders(Invited)
  • Place of Presentation: Chicago, USA
• [Presentation] Collaboration of BRCA1 and CtIP in cellular tolerance to topoisomerase poisons (2008)
  • Author(s): 武田俊一
  • Organizer: Gordon Research Conference(Invited)
  • Place of Presentation: Oxford, UK
• [Presentation] A critical role for the ubiquitin-conjugating enzymes Ubc13 and RNF8 in homologous recombination (2008)
  • Author(s): 武田俊一
  • Organizer: Genome Stability in Health and Disease(Invited)
  • Place of Presentation: Tel Aviv, Israel
• [Presentation] ニワトリBリンパ細胞を使ったDNA損傷応答機構の遺伝学的解析 (2008)
  • Author(s): 武田俊一
  • Organizer: 放射線防護センターシンポジウム(招待講演)
  • Place of Presentation: 稲毛,千葉